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P07000

- PLDB_ECOLI

UniProt

P07000 - PLDB_ECOLI

Protein

Lysophospholipase L2

Gene

pldB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 4 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    GO - Molecular functioni

    1. lysophospholipase activity Source: EcoliWiki
    2. protein binding Source: IntAct

    GO - Biological processi

    1. lipid metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10739-MONOMER.
    ECOL316407:JW5584-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysophospholipase L2 (EC:3.1.1.5)
    Alternative name(s):
    Lecithinase B
    Gene namesi
    Name:pldB
    Ordered Locus Names:b3825, JW5584
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10739. pldB.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: EcoliWiki
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 340340Lysophospholipase L2PRO_0000058456Add
    BLAST

    Proteomic databases

    PaxDbiP07000.
    PRIDEiP07000.

    Expressioni

    Gene expression databases

    GenevestigatoriP07000.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    mqsAQ468642EBI-9134416,EBI-1120353

    Protein-protein interaction databases

    IntActiP07000. 11 interactions.
    STRINGi511145.b3825.

    Structurei

    3D structure databases

    ProteinModelPortaliP07000.
    SMRiP07000. Positions 56-307.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG2267.
    HOGENOMiHOG000285163.
    KOiK01048.
    OMAiYHWVREG.
    OrthoDBiEOG6CCH34.
    PhylomeDBiP07000.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P07000-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFQQQKDWET RENAFAAFTM GPLTDFWRQR DEAEFTGVDD IPVRFVRFRA    50
    QHHDRVVVIC PGRIESYVKY AELAYDLFHL GFDVLIIDHR GQGRSGRLLA 100
    DPHLGHVNRF NDYVDDLAAF WQQEVQPGPW RKRYILAHSM GGAISTLFLQ 150
    RHPGVCDAIA LTAPMFGIVI RMPSFMARQI LNWAEAHPRF RDGYAIGTGR 200
    WRALPFAINV LTHSRQRYRR NLRFYADDPT IRVGGPTYHW VRESILAGEQ 250
    VLAGAGDDAT PTLLLQAEEE RVVDNRMHDR FCELRTAAGH PVEGGRPLVI 300
    KGAYHEILFE KDAMRSVALH AIVDFFNRHN SPSGNRSTEV 340
    Length:340
    Mass (Da):38,978
    Last modified:October 11, 2004 - v4
    Checksum:iA032DB05FD203C05
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti164 – 1641P → A in AAA67621. (PubMed:1379743)Curated
    Sequence conflicti315 – 3151R → A in AAA67621. (PubMed:1379743)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03155 Genomic DNA. Translation: CAA26932.1.
    M87049 Genomic DNA. Translation: AAA67621.1.
    U00096 Genomic DNA. Translation: AAT48224.1.
    AP009048 Genomic DNA. Translation: BAE77476.1.
    PIRiB65187. PSECL2.
    RefSeqiYP_026266.1. NC_000913.3.
    YP_491617.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAT48224; AAT48224; b3825.
    BAE77476; BAE77476; BAE77476.
    GeneIDi12933622.
    948314.
    KEGGiecj:Y75_p3353.
    eco:b3825.
    PATRICi32123151. VBIEscCol129921_3941.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03155 Genomic DNA. Translation: CAA26932.1 .
    M87049 Genomic DNA. Translation: AAA67621.1 .
    U00096 Genomic DNA. Translation: AAT48224.1 .
    AP009048 Genomic DNA. Translation: BAE77476.1 .
    PIRi B65187. PSECL2.
    RefSeqi YP_026266.1. NC_000913.3.
    YP_491617.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P07000.
    SMRi P07000. Positions 56-307.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P07000. 11 interactions.
    STRINGi 511145.b3825.

    Proteomic databases

    PaxDbi P07000.
    PRIDEi P07000.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT48224 ; AAT48224 ; b3825 .
    BAE77476 ; BAE77476 ; BAE77476 .
    GeneIDi 12933622.
    948314.
    KEGGi ecj:Y75_p3353.
    eco:b3825.
    PATRICi 32123151. VBIEscCol129921_3941.

    Organism-specific databases

    EchoBASEi EB0732.
    EcoGenei EG10739. pldB.

    Phylogenomic databases

    eggNOGi COG2267.
    HOGENOMi HOG000285163.
    KOi K01048.
    OMAi YHWVREG.
    OrthoDBi EOG6CCH34.
    PhylomeDBi P07000.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10739-MONOMER.
    ECOL316407:JW5584-MONOMER.

    Miscellaneous databases

    PROi P07000.

    Gene expression databases

    Genevestigatori P07000.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the pldB gene and characteristics of deduced amino acid sequence of lysophospholipase L2 in Escherichia coli."
      Kobayashi T., Kudo I., Karasawa K., Mizushima H., Inoue K., Nojima S.
      J. Biochem. 98:1017-1025(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / KL16-99.
    2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
      Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
      Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 164.
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: SEQUENCE REVISION TO 315.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiPLDB_ECOLI
    AccessioniPrimary (citable) accession number: P07000
    Secondary accession number(s): P78127, Q2M8D0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 121 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In addition this protein catalyzes a transacylation reaction to produce acyl phosphatidylglycerol by transfer of the acidyl residue of 2-acyl lysophospholipid to phosphatidylglycerol.
    Lysophospholipases from various sources vary widely in molecular weight, substrate specificity, and subcellular localization.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene

    External Data

    Dasty 3