Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P07000 (PLDB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysophospholipase L2

EC=3.1.1.5
Alternative name(s):
Lecithinase B
Gene names
Name:pldB
Ordered Locus Names:b3825, JW5584
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Subcellular location

Cell inner membrane; Peripheral membrane protein.

Miscellaneous

In addition this protein catalyzes a transacylation reaction to produce acyl phosphatidylglycerol by transfer of the acidyl residue of 2-acyl lysophospholipid to phosphatidylglycerol.

Lysophospholipases from various sources vary widely in molecular weight, substrate specificity, and subcellular localization.

Ontologies

Keywords
   Biological processLipid biosynthesis
Lipid metabolism
   Cellular componentCell inner membrane
Cell membrane
Membrane
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipid metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmembrane

Inferred from direct assay PubMed 3908447. Source: EcoliWiki

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlysophospholipase activity

Inferred from direct assay PubMed 3908447. Source: EcoliWiki

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

mqsAQ468642EBI-9134416,EBI-1120353

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Lysophospholipase L2
PRO_0000058456

Experimental info

Sequence conflict1641P → A in AAA67621. Ref.2
Sequence conflict3151R → A in AAA67621. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P07000 [UniParc].

Last modified October 11, 2004. Version 4.
Checksum: A032DB05FD203C05

FASTA34038,978
        10         20         30         40         50         60 
MFQQQKDWET RENAFAAFTM GPLTDFWRQR DEAEFTGVDD IPVRFVRFRA QHHDRVVVIC 

        70         80         90        100        110        120 
PGRIESYVKY AELAYDLFHL GFDVLIIDHR GQGRSGRLLA DPHLGHVNRF NDYVDDLAAF 

       130        140        150        160        170        180 
WQQEVQPGPW RKRYILAHSM GGAISTLFLQ RHPGVCDAIA LTAPMFGIVI RMPSFMARQI 

       190        200        210        220        230        240 
LNWAEAHPRF RDGYAIGTGR WRALPFAINV LTHSRQRYRR NLRFYADDPT IRVGGPTYHW 

       250        260        270        280        290        300 
VRESILAGEQ VLAGAGDDAT PTLLLQAEEE RVVDNRMHDR FCELRTAAGH PVEGGRPLVI 

       310        320        330        340 
KGAYHEILFE KDAMRSVALH AIVDFFNRHN SPSGNRSTEV 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the pldB gene and characteristics of deduced amino acid sequence of lysophospholipase L2 in Escherichia coli."
Kobayashi T., Kudo I., Karasawa K., Mizushima H., Inoue K., Nojima S.
J. Biochem. 98:1017-1025(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / KL16-99.
[2]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 164.
Strain: K12 / MG1655 / ATCC 47076.
[4]"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005."
Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.
Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 315.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03155 Genomic DNA. Translation: CAA26932.1.
M87049 Genomic DNA. Translation: AAA67621.1.
U00096 Genomic DNA. Translation: AAT48224.1.
AP009048 Genomic DNA. Translation: BAE77476.1.
PIRPSECL2. B65187.
RefSeqYP_026266.1. NC_000913.3.
YP_491617.1. NC_007779.1.

3D structure databases

ProteinModelPortalP07000.
SMRP07000. Positions 56-307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP07000. 11 interactions.
STRING511145.b3825.

Proteomic databases

PaxDbP07000.
PRIDEP07000.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT48224; AAT48224; b3825.
BAE77476; BAE77476; BAE77476.
GeneID12933622.
948314.
KEGGecj:Y75_p3353.
eco:b3825.
PATRIC32123151. VBIEscCol129921_3941.

Organism-specific databases

EchoBASEEB0732.
EcoGeneEG10739. pldB.

Phylogenomic databases

eggNOGCOG2267.
HOGENOMHOG000285163.
KOK01048.
OMAYHWVREG.
OrthoDBEOG6CCH34.
PhylomeDBP07000.
ProtClustDBPRK10749.

Enzyme and pathway databases

BioCycEcoCyc:EG10739-MONOMER.
ECOL316407:JW5584-MONOMER.

Gene expression databases

GenevestigatorP07000.

Family and domain databases

InterProIPR000073. AB_hydrolase_1.
[Graphical view]
PfamPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP07000.

Entry information

Entry namePLDB_ECOLI
AccessionPrimary (citable) accession number: P07000
Secondary accession number(s): P78127, Q2M8D0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene