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Protein

Lysophospholipase L2

Gene

pldB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

GO - Molecular functioni

  • lysophospholipase activity Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:EG10739-MONOMER.
ECOL316407:JW5584-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase L2 (EC:3.1.1.5)
Alternative name(s):
Lecithinase B
Gene namesi
Name:pldB
Ordered Locus Names:b3825, JW5584
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10739. pldB.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: EcoliWiki
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Lysophospholipase L2PRO_0000058456Add
BLAST

Proteomic databases

PaxDbiP07000.
PRIDEiP07000.

Expressioni

Gene expression databases

GenevestigatoriP07000.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
mqsAQ468642EBI-9134416,EBI-1120353

Protein-protein interaction databases

IntActiP07000. 11 interactions.
STRINGi511145.b3825.

Structurei

3D structure databases

ProteinModelPortaliP07000.
SMRiP07000. Positions 56-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG2267.
HOGENOMiHOG000285163.
InParanoidiP07000.
KOiK01048.
OMAiYHWVREG.
OrthoDBiEOG6CCH34.
PhylomeDBiP07000.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P07000-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQQQKDWET RENAFAAFTM GPLTDFWRQR DEAEFTGVDD IPVRFVRFRA
60 70 80 90 100
QHHDRVVVIC PGRIESYVKY AELAYDLFHL GFDVLIIDHR GQGRSGRLLA
110 120 130 140 150
DPHLGHVNRF NDYVDDLAAF WQQEVQPGPW RKRYILAHSM GGAISTLFLQ
160 170 180 190 200
RHPGVCDAIA LTAPMFGIVI RMPSFMARQI LNWAEAHPRF RDGYAIGTGR
210 220 230 240 250
WRALPFAINV LTHSRQRYRR NLRFYADDPT IRVGGPTYHW VRESILAGEQ
260 270 280 290 300
VLAGAGDDAT PTLLLQAEEE RVVDNRMHDR FCELRTAAGH PVEGGRPLVI
310 320 330 340
KGAYHEILFE KDAMRSVALH AIVDFFNRHN SPSGNRSTEV
Length:340
Mass (Da):38,978
Last modified:October 11, 2004 - v4
Checksum:iA032DB05FD203C05
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641P → A in AAA67621 (PubMed:1379743).Curated
Sequence conflicti315 – 3151R → A in AAA67621 (PubMed:1379743).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03155 Genomic DNA. Translation: CAA26932.1.
M87049 Genomic DNA. Translation: AAA67621.1.
U00096 Genomic DNA. Translation: AAT48224.1.
AP009048 Genomic DNA. Translation: BAE77476.1.
PIRiB65187. PSECL2.
RefSeqiYP_026266.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48224; AAT48224; b3825.
BAE77476; BAE77476; BAE77476.
GeneIDi948314.
KEGGiecj:Y75_p3353.
eco:b3825.
PATRICi32123151. VBIEscCol129921_3941.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03155 Genomic DNA. Translation: CAA26932.1.
M87049 Genomic DNA. Translation: AAA67621.1.
U00096 Genomic DNA. Translation: AAT48224.1.
AP009048 Genomic DNA. Translation: BAE77476.1.
PIRiB65187. PSECL2.
RefSeqiYP_026266.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP07000.
SMRiP07000. Positions 56-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP07000. 11 interactions.
STRINGi511145.b3825.

Proteomic databases

PaxDbiP07000.
PRIDEiP07000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48224; AAT48224; b3825.
BAE77476; BAE77476; BAE77476.
GeneIDi948314.
KEGGiecj:Y75_p3353.
eco:b3825.
PATRICi32123151. VBIEscCol129921_3941.

Organism-specific databases

EchoBASEiEB0732.
EcoGeneiEG10739. pldB.

Phylogenomic databases

eggNOGiCOG2267.
HOGENOMiHOG000285163.
InParanoidiP07000.
KOiK01048.
OMAiYHWVREG.
OrthoDBiEOG6CCH34.
PhylomeDBiP07000.

Enzyme and pathway databases

BioCyciEcoCyc:EG10739-MONOMER.
ECOL316407:JW5584-MONOMER.

Miscellaneous databases

PROiP07000.

Gene expression databases

GenevestigatoriP07000.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the pldB gene and characteristics of deduced amino acid sequence of lysophospholipase L2 in Escherichia coli."
    Kobayashi T., Kudo I., Karasawa K., Mizushima H., Inoue K., Nojima S.
    J. Biochem. 98:1017-1025(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / KL16-99.
  2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 164.
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: SEQUENCE REVISION TO 315.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPLDB_ECOLI
AccessioniPrimary (citable) accession number: P07000
Secondary accession number(s): P78127, Q2M8D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 11, 2004
Last modified: May 27, 2015
This is version 124 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In addition this protein catalyzes a transacylation reaction to produce acyl phosphatidylglycerol by transfer of the acidyl residue of 2-acyl lysophospholipid to phosphatidylglycerol.
Lysophospholipases from various sources vary widely in molecular weight, substrate specificity, and subcellular localization.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.