6-phosphofructokinase isozyme 2 - Escherichia coli (strain K12)

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P06999 (K6PF2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
6-phosphofructokinase isozyme 2
Short name=Phosphofructokinase-2
EC=2.7.1.11

Gene names

Name:	pfkB
Ordered Locus Names:	b1723, JW5280

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	PFK-2 is sensitive to inhibition by fructose 1,6-diphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Homodimer.
Miscellaneous	Only 10% of the activity present in the wild-type strain is phosphofructokinase-2. This enzyme is not to be confused with 6-phosphofructo-2-kinase which is also called phosphofructokinase 2. E.coli has two 6-phosphofructokinases enzymes.
Sequence similarities	Belongs to the carbohydrate kinase PfkB family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from mutant phenotype PubMed 125264. Source: EcoCyc response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Molecular_function	6-phosphofructokinase activity Inferred from direct assay PubMed 149128. Source: EcoCyc ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from direct assay PubMed 16866375. Source: EcoCyc tagatose-6-phosphate kinase activity Inferred from direct assay PubMed 149128. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 309

309

6-phosphofructokinase isozyme 2

PRO_0000080083

Experimental info

Sequence conflict

26 – 38

GKLRC…VFEPG → ENCAVPHRCSNP Ref.1

Sequence conflict

26 – 38

GKLRC…VFEPG → ENCAVPHRCSNP Ref.5

Sequence conflict

155 – 171

AAQKQ…SSGEA → LRKNKGSAASSTVLGQG in AAA24320. Ref.1

Sequence conflict

245 – 246

PV → AL in AAA24321. Ref.1

Sequence conflict

257 – 258

SM → RL in AAA24321. Ref.1

Secondary structure

309

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P06999 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: A93BEBE0D5801309
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FASTA

309

32,456

        10         20         30         40         50         60 
MVRIYTLTLA PSLDSATITP QIYPEGKLRC TAPVFEPGGG GINVARAIAH LGGSATAIFP 

        70         80         90        100        110        120 
AGGATGEHLV SLLADENVPV ATVEAKDWTR QNLHVHVEAS GEQYRFVMPG AALNEDEFRQ 

       130        140        150        160        170        180 
LEEQVLEIES GAILVISGSL PPGVKLEKLT QLISAAQKQG IRCIVDSSGE ALSAALAIGN 

       190        200        210        220        230        240 
IELVKPNQKE LSALVNRELT QPDDVRKAAQ EIVNSGKAKR VVVSLGPQGA LGVDSENCIQ 

       250        260        270        280        290        300 
VVPPPVKSQS TVGAGDSMVG AMTLKLAENA SLEEMVRFGV AAGSAATLNQ GTRLCSHDDT 


QKIYAYLSR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of gene pfkB encoding the minor phosphofructokinase of Escherichia coli K-12." Daldal F. Gene 28:337-342(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme." Daldal F. J. Mol. Biol. 168:285-305(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
[6]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K02500 Genomic DNA. Translation: AAA24321.1.
U00096 Genomic DNA. Translation: AAC74793.1.
AP009048 Genomic DNA. Translation: BAA15500.2.
K00128 Genomic DNA. Translation: AAA24320.1.

PIR

KIECFB. C64931.

RefSeq

NP_416237.3. NC_000913.2.
YP_489984.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CQD	X-ray	1.98	A/B	1-309	[»]
3N1C	X-ray	2.00	A/B/C/D	1-309	[»]
3UMO	X-ray	1.70	A/B	1-309	[»]
3UMP	X-ray	1.85	A/B	1-309	[»]
3UQD	X-ray	2.14	A/B/C/D	1-309	[»]
3UQE	X-ray	2.20	A/B	1-309	[»]

ProteinModelPortal

P06999.

SMR

P06999. Positions 1-309.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10465N.

STRING

511145.b1723.

2D gel databases

SWISS-2DPAGE

P06999.

Proteomic databases

PaxDb

P06999.

PRIDE

P06999.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74793; AAC74793; b1723.
BAA15500; BAA15500; BAA15500.

GeneID

12934082.
946230.

KEGG

ecj:Y75_p1698.
eco:b1723.

PATRIC

32118753. VBIEscCol129921_1794.

Organism-specific databases

EchoBASE

EB0694.

EcoGene

EG10700. pfkB.

Phylogenomic databases

eggNOG

COG1105.

HOGENOM

HOG000265281.

K16370.

OMA

PMKSQST.

ProtClustDB

PRK10294.

Enzyme and pathway databases

BioCyc

EcoCyc:6PFK-2-MONOMER.
ECOL316407:JW5280-MONOMER.
MetaCyc:6PFK-2-MONOMER.

SABIO-RK

P06999.

UniPathway

UPA00109; UER00182.

Gene expression databases

Genevestigator

P06999.

Family and domain databases

InterPro

IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR017583. Tagatose/fructose_Pkinase.
[Graphical view]

Pfam

PF00294. PfkB. 1 hit.
[Graphical view]

PIRSF

PIRSF000535. 1PFK/6PFK/LacC. 1 hit.

TIGRFAMs

TIGR03168. 1-PFK. 1 hit.

PROSITE

PS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P06999.

Entry information

Entry name

K6PF2_ECOLI

Accession

Primary (citable) accession number: P06999
Secondary accession number(s): P78065, P78260

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents