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P06999 (K6PF2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphofructokinase isozyme 2

Short name=Phosphofructokinase-2
EC=2.7.1.11
Gene names
Name:pfkB
Ordered Locus Names:b1723, JW5280
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Enzyme regulation

PFK-2 is sensitive to inhibition by fructose 1,6-diphosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Subunit structure

Homodimer.

Miscellaneous

Only 10% of the activity present in the wild-type strain is phosphofructokinase-2.

This enzyme is not to be confused with 6-phosphofructo-2-kinase which is also called phosphofructokinase 2.

E.coli has two 6-phosphofructokinases enzymes.

Sequence similarities

Belongs to the carbohydrate kinase PfkB family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-6966085,EBI-6966085

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3093096-phosphofructokinase isozyme 2
PRO_0000080083

Experimental info

Sequence conflict26 – 3813GKLRC…VFEPG → ENCAVPHRCSNP Ref.1
Sequence conflict26 – 3813GKLRC…VFEPG → ENCAVPHRCSNP Ref.5
Sequence conflict155 – 17117AAQKQ…SSGEA → LRKNKGSAASSTVLGQG in AAA24320. Ref.1
Sequence conflict245 – 2462PV → AL in AAA24321. Ref.1
Sequence conflict257 – 2582SM → RL in AAA24321. Ref.1

Secondary structure

...................................................... 309
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06999 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: A93BEBE0D5801309

FASTA30932,456
        10         20         30         40         50         60 
MVRIYTLTLA PSLDSATITP QIYPEGKLRC TAPVFEPGGG GINVARAIAH LGGSATAIFP 

        70         80         90        100        110        120 
AGGATGEHLV SLLADENVPV ATVEAKDWTR QNLHVHVEAS GEQYRFVMPG AALNEDEFRQ 

       130        140        150        160        170        180 
LEEQVLEIES GAILVISGSL PPGVKLEKLT QLISAAQKQG IRCIVDSSGE ALSAALAIGN 

       190        200        210        220        230        240 
IELVKPNQKE LSALVNRELT QPDDVRKAAQ EIVNSGKAKR VVVSLGPQGA LGVDSENCIQ 

       250        260        270        280        290        300 
VVPPPVKSQS TVGAGDSMVG AMTLKLAENA SLEEMVRFGV AAGSAATLNQ GTRLCSHDDT 


QKIYAYLSR 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of gene pfkB encoding the minor phosphofructokinase of Escherichia coli K-12."
Daldal F.
Gene 28:337-342(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme."
Daldal F.
J. Mol. Biol. 168:285-305(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
[6]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02500 Genomic DNA. Translation: AAA24321.1.
U00096 Genomic DNA. Translation: AAC74793.1.
AP009048 Genomic DNA. Translation: BAA15500.2.
K00128 Genomic DNA. Translation: AAA24320.1.
PIRKIECFB. C64931.
RefSeqNP_416237.3. NC_000913.3.
YP_489984.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CQDX-ray1.98A/B1-309[»]
3N1CX-ray2.00A/B/C/D1-309[»]
3UMOX-ray1.70A/B1-309[»]
3UMPX-ray1.85A/B1-309[»]
3UQDX-ray2.14A/B/C/D1-309[»]
3UQEX-ray2.20A/B1-309[»]
ProteinModelPortalP06999.
SMRP06999. Positions 1-309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10465N.
MINTMINT-8178151.
STRING511145.b1723.

2D gel databases

SWISS-2DPAGEP06999.

Proteomic databases

PaxDbP06999.
PRIDEP06999.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74793; AAC74793; b1723.
BAA15500; BAA15500; BAA15500.
GeneID12934082.
946230.
KEGGecj:Y75_p1698.
eco:b1723.
PATRIC32118753. VBIEscCol129921_1794.

Organism-specific databases

EchoBASEEB0694.
EcoGeneEG10700. pfkB.

Phylogenomic databases

eggNOGCOG1105.
HOGENOMHOG000265281.
KOK16370.
OMANTMEAET.
OrthoDBEOG61KBKF.
PhylomeDBP06999.
ProtClustDBPRK10294.

Enzyme and pathway databases

BioCycEcoCyc:6PFK-2-MONOMER.
ECOL316407:JW5280-MONOMER.
MetaCyc:6PFK-2-MONOMER.
SABIO-RKP06999.
UniPathwayUPA00109; UER00182.

Gene expression databases

GenevestigatorP06999.

Family and domain databases

InterProIPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR017583. Tagatose/fructose_Pkinase.
[Graphical view]
PfamPF00294. PfkB. 1 hit.
[Graphical view]
PIRSFPIRSF000535. 1PFK/6PFK/LacC. 1 hit.
TIGRFAMsTIGR03168. 1-PFK. 1 hit.
PROSITEPS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP06999.
PROP06999.

Entry information

Entry nameK6PF2_ECOLI
AccessionPrimary (citable) accession number: P06999
Secondary accession number(s): P78065, P78260
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene