6-phosphofructokinase isozyme 2 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P06999 (K6PF2_ECOLI)

Last modified June 16, 2009. Version 84. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    6-phosphofructokinase isozyme 2
      Short name=Phosphofructokinase-2
    EC=2.7.1.11

Gene names

Name:	pfkB
Ordered Locus Names:	b1723, JW5280

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	PFK-2 is sensitive to inhibition by fructose 1,6-diphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Homodimer.
Miscellaneous	Only 10% of the activity present in the wild-type strain is phosphofructokinase-2. This enzyme is not to be confused with 6-phosphofructo-2-kinase which is also called phosphofructokinase 2. E.coli has two 6-phosphofructokinases enzymes.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

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Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 309

309

6-phosphofructokinase isozyme 2

PRO_0000080083

Experimental info

Sequence conflict

26 – 38

GKLRC…VFEPG → ENCAVPHRCSNP Ref.1

Sequence conflict

26 – 38

GKLRC…VFEPG → ENCAVPHRCSNP Ref.5

Sequence conflict

155 – 171

AAQKQ…SSGEA → LRKNKGSAASSTVLGQG in AAA24320. Ref.1

Sequence conflict

245 – 246

PV → AL in AAA24321. Ref.1

Sequence conflict

257 – 258

SM → RL in AAA24321. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P06999-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: A93BEBE0D5801309
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FASTA

309

32,456

        10         20         30         40         50         60 
MVRIYTLTLA PSLDSATITP QIYPEGKLRC TAPVFEPGGG GINVARAIAH LGGSATAIFP 

        70         80         90        100        110        120 
AGGATGEHLV SLLADENVPV ATVEAKDWTR QNLHVHVEAS GEQYRFVMPG AALNEDEFRQ 

       130        140        150        160        170        180 
LEEQVLEIES GAILVISGSL PPGVKLEKLT QLISAAQKQG IRCIVDSSGE ALSAALAIGN 

       190        200        210        220        230        240 
IELVKPNQKE LSALVNRELT QPDDVRKAAQ EIVNSGKAKR VVVSLGPQGA LGVDSENCIQ 

       250        260        270        280        290        300 
VVPPPVKSQS TVGAGDSMVG AMTLKLAENA SLEEMVRFGV AAGSAATLNQ GTRLCSHDDT 


QKIYAYLSR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of gene pfkB encoding the minor phosphofructokinase of Escherichia coli K-12." Daldal F. Gene 28:337-342(1984) [PubMed: 6235149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme." Daldal F. J. Mol. Biol. 168:285-305(1983) [PubMed: 6310120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

K02500 Genomic DNA. Translation: AAA24321.1.
U00096 Genomic DNA. Translation: AAC74793.1.
AP009048 Genomic DNA. Translation: BAA15500.2.
K00128 Genomic DNA. Translation: AAA24320.1.

PIR

KIECFB. C64931.

RefSeq

AP_002342.1.
NP_416237.3.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CQD	X-ray	1.98	A/B	1-309	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:10465N.

2-D gel databases

SWISS-2DPAGE

P06999.

2DBase-Ecoli

P06999.

ECO2DBASE

E036.6. 6TH EDITION.

Genome annotation databases

GeneID

946230.

GenomeReviews

Gene locus JW5280 in contig AP009048_GR.
Gene locus b1723 in contig U00096_GR.

KEGG

ecj:JW5280.
eco:b1723.

Organism-specific databases

EchoBASE

EB0694.

EcoGene

EG10700. pfkB.

CMR

Search...

Phylogenomic databases

HOGENOM

P06999.

OMA

P06999. TRENFSV.

Enzyme and pathway databases

BioCyc

EcoCyc:6PFK-2-MON.
MetaCyc:6PFK-2-MON.

Family and domain databases

InterPro

IPR017583. 1-phosphofructokinase.
IPR011611. Carb/pur_kinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
[Graphical view]

Pfam

PF00294. PfkB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03168. 1-PFK. 1 hit.

PROSITE

PS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

K6PF2_ECOLI

Accession

Primary (citable) accession number: P06999
Secondary accession number(s): P78065, P78260

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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