Reviewed,
UniProtKB/Swiss-Prot P06999 (K6PF2_ECOLI)
Last modified
June 16, 2009.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 6-phosphofructokinase isozyme 2 Short name=Phosphofructokinase-2 EC=2.7.1.11 | ||||
| Gene names |
| ||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 309 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. |
| Enzyme regulation | PFK-2 is sensitive to inhibition by fructose 1,6-diphosphate. |
| Pathway | |
| Subunit structure | Homodimer. |
| Miscellaneous | Only 10% of the activity present in the wild-type strain is phosphofructokinase-2. This enzyme is not to be confused with 6-phosphofructo-2-kinase which is also called phosphofructokinase 2. E.coli has two 6-phosphofructokinases enzymes. |
| Sequence similarities | Belongs to the carbohydrate kinase pfkB family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Transferase |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 309 | 309 | 6-phosphofructokinase isozyme 2 | PRO_0000080083 | |||||
Experimental info | |||||||||
| Sequence conflict | 26 – 38 | 13 | GKLRC…VFEPG → ENCAVPHRCSNP Ref.1 | ||||||
| Sequence conflict | 26 – 38 | 13 | GKLRC…VFEPG → ENCAVPHRCSNP Ref.5 | ||||||
| Sequence conflict | 155 – 171 | 17 | AAQKQ…SSGEA → LRKNKGSAASSTVLGQG in AAA24320. Ref.1 | ||||||
| Sequence conflict | 245 – 246 | 2 | PV → AL in AAA24321. Ref.1 | ||||||
| Sequence conflict | 257 – 258 | 2 | SM → RL in AAA24321. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence of gene pfkB encoding the minor phosphofructokinase of Escherichia coli K-12." Daldal F. Gene 28:337-342(1984) [PubMed: 6235149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T.DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme." Daldal F. J. Mol. Biol. 168:285-305(1983) [PubMed: 6310120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| K02500 Genomic DNA. Translation: AAA24321.1. U00096 Genomic DNA. Translation: AAC74793.1. AP009048 Genomic DNA. Translation: BAA15500.2. K00128 Genomic DNA. Translation: AAA24320.1. | |||||||||||||
| PIR | KIECFB. C64931. | ||||||||||||
| RefSeq | AP_002342.1. NP_416237.3. | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP:10465N. | ||||||||||||
2-D gel databases | |||||||||||||
| SWISS-2DPAGE | P06999. | ||||||||||||
| 2DBase-Ecoli | P06999. | ||||||||||||
| ECO2DBASE | E036.6. 6TH EDITION. | ||||||||||||
Genome annotation databases | |||||||||||||
| GeneID | 946230. | ||||||||||||
| GenomeReviews | Gene locus JW5280 in contig AP009048_GR. Gene locus b1723 in contig U00096_GR. | ||||||||||||
| KEGG | ecj:JW5280. eco:b1723. | ||||||||||||
Organism-specific databases | |||||||||||||
| EchoBASE | EB0694. | ||||||||||||
| EcoGene | EG10700. pfkB. | ||||||||||||
| CMR | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | P06999. | ||||||||||||
| OMA | P06999. TRENFSV. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BioCyc | EcoCyc:6PFK-2-MON. MetaCyc:6PFK-2-MON. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR017583. 1-phosphofructokinase. IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. [Graphical view] | ||||||||||||
| Pfam | PF00294. PfkB. 1 hit. [Graphical view] | ||||||||||||
| TIGRFAMs | TIGR03168. 1-PFK. 1 hit. | ||||||||||||
| PROSITE | PS00583. PFKB_KINASES_1. 1 hit. PS00584. PFKB_KINASES_2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Entry information
| Entry name | K6PF2_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P06999 Secondary accession number(s): P78065, P78260 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


