Outer membrane protein C precursor - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P06996 (OMPC_ECOLI)

Last modified January 19, 2010. Version 103. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Outer membrane protein C
Alternative name(s):
    Porin ompC
    Outer membrane protein 1B

Gene names

Name:	ompC
Synonyms:	meoA, par
Ordered Locus Names:	b2215, JW2203

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Forms passive diffusion pores which allow small molecular weight hydrophilic materials across the outer membrane.
Subunit structure	Homotrimer.
Subcellular location	Cell outer membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the Gram-negative porin family.

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Ontologies

Keywords
Biological process	Ion transport Phage recognition Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal Transmembrane
Molecular function	Porin
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	detection of virus Inferred from electronic annotation. Source: UniProtKB-KW entry of virus into host cell Inferred from electronic annotation. Source: UniProtKB-KW ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW pore complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

Ref.6 Ref.8 Ref.9

Chain

22 – 367

346

Outer membrane protein C

PRO_0000025230

Secondary structure

367

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P06996-1 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 6A49370CC8A1A225
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FASTA

367

40,368

        10         20         30         40         50         60 
MKVKVLSLLV PALLVAGAAN AAEVYNKDGN KLDLYGKVDG LHYFSDNKDV DGDQTYMRLG 

        70         80         90        100        110        120 
FKGETQVTDQ LTGYGQWEYQ IQGNSAENEN NSWTRVAFAG LKFQDVGSFD YGRNYGVVYD 

       130        140        150        160        170        180 
VTSWTDVLPE FGGDTYGSDN FMQQRGNGFA TYRNTDFFGL VDGLNFAVQY QGKNGNPSGE 

       190        200        210        220        230        240 
GFTSGVTNNG RDALRQNGDG VGGSITYDYE GFGIGGAISS SKRTDAQNTA AYIGNGDRAE 

       250        260        270        280        290        300 
TYTGGLKYDA NNIYLAAQYT QTYNATRVGS LGWANKAQNF EAVAQYQFDF GLRPSLAYLQ 

       310        320        330        340        350        360 
SKGKNLGRGY DDEDILKYVD VGATYYFNKN MSTYVDYKIN LLDDNQFTRD AGINTDNIVA 


LGLVYQF

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A comparative study on the genes for three porins of the Escherichia coli outer membrane. DNA sequence of the osmoregulated ompC gene." Mizuno T., Chou M.-Y., Inouye M. J. Biol. Chem. 258:6932-6940(1983) [PubMed: 6304064] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 218-367. Strain: K12 / BHB2600.
[6]	"DNA sequence of the promoter region of the ompC gene and the amino acid sequence of the signal peptide of pro-OmpC protein of Escherichia coli." Mizuno T., Chou M.-Y., Inouye M. FEBS Lett. 151:159-164(1983) [PubMed: 6297988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22, PROTEIN SEQUENCE OF 22-40.
[7]	"Construction of a series of ompF-ompC chimeric genes by in vivo homologous recombination in Escherichia coli and characterization of the translational products." Nogami T., Mizuno T., Mizushima S. J. Bacteriol. 164:797-801(1985) [PubMed: 2997131] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-57.
[8]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-30. Strain: K12 / EMG2.
[9]	"Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis." Molloy M.P., Herbert B.R., Walsh B.J., Tyler M.I., Traini M., Sanchez J.-C., Hochstrasser D.F., Williams K.L., Gooley A.A. Electrophoresis 19:837-844(1998) [PubMed: 9629924] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-26. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K00541 Genomic DNA. Translation: AAA24243.1.
U00096 Genomic DNA. Translation: AAC75275.1.
AP009048 Genomic DNA. Translation: BAA15998.1.
U00008 Genomic DNA. Translation: AAA16412.1.

PIR

MMECPC. A20867.

RefSeq

AP_002811.1.
NP_416719.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J1N	X-ray	2.00	A/B/C	22-367	[»]
2J4U	X-ray	2.99	P/Q/R/U/V/W	22-367	[»]
2ZLE	electron microscopy	28.00	D	22-367	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10397N.

STRING

P06996.

Protein family/group databases

TCDB

1.B.1.1.3. general bacterial porin (GBP) family.

2-D gel databases

2DBase-Ecoli

P06996.

ECO2DBASE

A035.5. 6TH EDITION.

Proteomic databases

PRIDE

P06996.

Genome annotation databases

GeneID

946716.

GenomeReviews

Gene locus JW2203 in contig AP009048_GR.
Gene locus b2215 in contig U00096_GR.

KEGG

ecj:JW2203.
eco:b2215.

Organism-specific databases

EchoBASE

EB0664.

EcoGene

EG10670. ompC.

CMR

Search...

Phylogenomic databases

eggNOG

COG3203.

HOGENOM

HBG673855.

OMA

NAPESEN.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10670-MONOMER.
ECOL168927:B2215-MONOMER.

Gene expression databases

Genevestigator

P06996.

Family and domain databases

InterPro

IPR001897. Porin_bac.
IPR013793. Porin_general_Gram-ve_CS.
IPR001702. Porin_Gram-ve.
[Graphical view]

Gene3D

G3DSA:2.40.160.10. Porin_Gram-ve. 1 hit.

Pfam

PF00267. Porin_1. 1 hit.
[Graphical view]

PRINTS

PR00183. ECOLIPORIN.
PR00182. ECOLNEIPORIN.

PROSITE

PS00576. GRAM_NEG_PORIN. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

OMPC_ECOLI

Accession

Primary (citable) accession number: P06996

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	January 19, 2010
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families