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Protein

Histidine biosynthesis bifunctional protein HisIE

Gene

hisI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate.
1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoribosyl-AMP cyclohydrolase activity Source: EcoCyc
  • phosphoribosyl-ATP diphosphatase activity Source: EcoCyc

GO - Biological processi

  • histidine biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Histidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:HISTCYCLOPRATPPHOS
MetaCyc:HISTCYCLOPRATPPHOS
UniPathwayiUPA00031; UER00007
UPA00031; UER00008

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine biosynthesis bifunctional protein HisIE
Including the following 2 domains:
Phosphoribosyl-AMP cyclohydrolase (EC:3.5.4.19)
Short name:
PRA-CH
Phosphoribosyl-ATP pyrophosphatase (EC:3.6.1.31)
Short name:
PRA-PH
Gene namesi
Name:hisI
Synonyms:hisIE
Ordered Locus Names:b2026, JW2008
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10451 hisI

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001364111 – 203Histidine biosynthesis bifunctional protein HisIEAdd BLAST203

Proteomic databases

PaxDbiP06989
PRIDEiP06989

Interactioni

Protein-protein interaction databases

BioGridi4260421, 19 interactors
DIPiDIP-9907N
IntActiP06989, 15 interactors
STRINGi316385.ECDH10B_2174

Structurei

3D structure databases

ProteinModelPortaliP06989
SMRiP06989
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 114Phosphoribosyl-AMP cyclohydrolaseAdd BLAST114
Regioni115 – 203Phosphoribosyl-ATP pyrophosphohydrolaseAdd BLAST89

Sequence similaritiesi

In the N-terminal section; belongs to the PRA-CH family.Curated
In the C-terminal section; belongs to the PRA-PH family.Curated

Phylogenomic databases

eggNOGiENOG4105K8F Bacteria
COG0139 LUCA
COG0140 LUCA
HOGENOMiHOG000277503
InParanoidiP06989
KOiK11755
OMAiVMACKDD
PhylomeDBiP06989

Family and domain databases

CDDicd11534 NTP-PPase_HisIE_like, 1 hit
Gene3Di3.10.20.400, 1 hit
HAMAPiMF_01020 HisE, 1 hit
MF_01019 HisIE, 1 hit
InterProiView protein in InterPro
IPR023019 His_synth_HisIE
IPR008179 HisE
IPR021130 PRib-ATP_PPHydrolase-like
IPR002496 PRib_AMP_CycHydrolase_dom
IPR038019 PRib_AMP_CycHydrolase_sf
PfamiView protein in Pfam
PF01502 PRA-CH, 1 hit
PF01503 PRA-PH, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002610 PRA_CycHdrlase, 1 hit
SUPFAMiSSF141734 SSF141734, 1 hit
TIGRFAMsiTIGR03188 histidine_hisI, 1 hit

Sequencei

Sequence statusi: Complete.

P06989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTEQQRREL DWEKTDGLMP VIVQHAVSGE VLMLGYMNPE ALDKTLESGK
60 70 80 90 100
VTFFSRTKQR LWTKGETSGN FLNVVSIAPD CDNDTLLVLA NPIGPTCHKG
110 120 130 140 150
TSSCFGDTAH QWLFLYQLEQ LLAERKSADP ETSYTAKLYA SGTKRIAQKV
160 170 180 190 200
GEEGVETALA ATVHDRFELT NEASDLMYHL LVLLQDQGLD LTTVIENLRK

RHQ
Length:203
Mass (Da):22,756
Last modified:February 1, 1995 - v3
Checksum:i34019009D82E0122
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68S → P in CAA31818 (PubMed:3062174).Curated1
Sequence conflicti193T → P (PubMed:3062174).Curated1
Sequence conflicti193T → P (PubMed:3018428).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti5Q → L in strain: F719. 1
Natural varianti46L → I in strain: F719. 1
Natural varianti164H → N in strain: F719. 1
Natural varianti192 – 193TT → GE in strain: F719. 2
Natural varianti196E → D in strain: F719. 1
Natural varianti199 – 200RK → KN in strain: F719. 2
Natural varianti203Missing in strain: F719. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13462 Genomic DNA Translation: CAA31818.1
X03974 Genomic DNA Translation: CAA27613.1
U02072 Unassigned DNA Translation: AAA19744.1
D43637 Genomic DNA Translation: BAA07753.1
U00096 Genomic DNA Translation: AAC75087.1
AP009048 Genomic DNA Translation: BAA15858.1
PIRiJS0135 YNECHI
RefSeqiNP_416530.1, NC_000913.3
WP_000954911.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75087; AAC75087; b2026
BAA15858; BAA15858; BAA15858
GeneIDi946515
KEGGiecj:JW2008
eco:b2026
PATRICifig|1411691.4.peg.226

Similar proteinsi

Entry informationi

Entry nameiHIS2_ECOLI
AccessioniPrimary (citable) accession number: P06989
Secondary accession number(s): P78079, Q47596
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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