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P06974

- FLIM_ECOLI

UniProt

P06974 - FLIM_ECOLI

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Protein

Flagellar motor switch protein FliM

Gene
fliM, cheC2, fla AII, fla QII, b1945, JW1929
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.

GO - Molecular functioni

  1. motor activity Source: InterPro
  2. protein binding Source: IntAct

GO - Biological processi

  1. bacterial-type flagellum-dependent cell motility Source: InterPro
  2. chemotaxis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCyciEcoCyc:FLIM-FLAGELLAR-C-RING-SWITCH.
ECOL316407:JW1929-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar motor switch protein FliM
Gene namesi
Name:fliM
Synonyms:cheC2, fla AII, fla QII
Ordered Locus Names:b1945, JW1929
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10323. fliM.

Subcellular locationi

GO - Cellular componenti

  1. bacterial-type flagellum basal body Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Bacterial flagellum, Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi153 – 1531V → D: Decreases binding to YcgR. 1 Publication
Mutagenesisi155 – 1551N → E: Decreases binding to YcgR. 1 Publication
Mutagenesisi156 – 1561R → D: Obviates binding to YcgR. 1 Publication
Mutagenesisi160 – 1601L → E: Obviates binding to YcgR. 1 Publication
Mutagenesisi167 – 1671D → R: Decreases binding to YcgR. 1 Publication
Mutagenesisi176 – 1761E → R: Decreases binding to YcgR. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Flagellar motor switch protein FliMPRO_0000180928Add
BLAST

Proteomic databases

PaxDbiP06974.
PRIDEiP06974.

Expressioni

Gene expression databases

GenevestigatoriP06974.

Interactioni

Subunit structurei

Interacts with flagellar brake protein YcgR.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
fliGP0ABZ116EBI-560439,EBI-1126524
fliNP150707EBI-560439,EBI-2011987
ycgRP760103EBI-560439,EBI-554507

Protein-protein interaction databases

DIPiDIP-1100N.
IntActiP06974. 18 interactions.
MINTiMINT-1301311.
STRINGi511145.b1945.

Structurei

Secondary structure

1
334
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 158

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F4VX-ray2.22D/E/F1-16[»]
1U8TX-ray1.50E/F1-16[»]
2B1JX-ray2.40C/D1-16[»]
ProteinModelPortaliP06974.
SMRiP06974. Positions 48-229.

Miscellaneous databases

EvolutionaryTraceiP06974.

Family & Domainsi

Sequence similaritiesi

Belongs to the FliM family.

Phylogenomic databases

eggNOGiCOG1868.
HOGENOMiHOG000253580.
KOiK02416.
OMAiLFNMMRR.
OrthoDBiEOG68M4DH.
PhylomeDBiP06974.

Family and domain databases

InterProiIPR028976. CheC-like_dom.
IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]
PfamiPF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view]
PIRSFiPIRSF002888. FliM. 1 hit.
PRINTSiPR00955. FLGMOTORFLIM.
SUPFAMiSSF101801. SSF101801. 1 hit.
SSF103039. SSF103039. 1 hit.
TIGRFAMsiTIGR01397. fliM_switch. 1 hit.

Sequencei

Sequence statusi: Complete.

P06974-1 [UniParc]FASTAAdd to Basket

« Hide

MGDSILSQAE IDALLNGDSE VKDEPTASVS GESDIRPYDP NTQRRVVRER    50
LQALEIINER FARHFRMGLF NLLRRSPDIT VGAIRIQPYH EFARNLPVPT 100
NLNLIHLKPL RGTGLVVFSP SLVFIAVDNL FGGDGRFPTK VEGREFTHTE 150
QRVINRMLKL ALEGYSDAWK AINPLEVEYV RSEMQVKFTN ITTSPNDIVV 200
NTPFHVEIGN LTGEFNICLP FSMIEPLREL LVNPPLENSR NEDQNWRDNL 250
VRQVQHSQLE LVANFADISL RLSQILKLNP GDVLPIEKPD RIIAHVDGVP 300
VLTSQYGTLN GQYALRIEHL INPILNSLNE EQPK 334
Length:334
Mass (Da):37,849
Last modified:April 1, 1988 - v1
Checksum:i05DC012B9364BFD6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12784 Genomic DNA. Translation: AAA23786.1.
U00096 Genomic DNA. Translation: AAC75012.1.
AP009048 Genomic DNA. Translation: BAA15770.1.
M26294 Unassigned DNA. No translation available.
PIRiB29842. XMECF2.
RefSeqiNP_416455.1. NC_000913.3.
YP_490199.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75012; AAC75012; b1945.
BAA15770; BAA15770; BAA15770.
GeneIDi12932576.
946442.
KEGGiecj:Y75_p1915.
eco:b1945.
PATRICi32119219. VBIEscCol129921_2024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12784 Genomic DNA. Translation: AAA23786.1 .
U00096 Genomic DNA. Translation: AAC75012.1 .
AP009048 Genomic DNA. Translation: BAA15770.1 .
M26294 Unassigned DNA. No translation available.
PIRi B29842. XMECF2.
RefSeqi NP_416455.1. NC_000913.3.
YP_490199.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F4V X-ray 2.22 D/E/F 1-16 [» ]
1U8T X-ray 1.50 E/F 1-16 [» ]
2B1J X-ray 2.40 C/D 1-16 [» ]
ProteinModelPortali P06974.
SMRi P06974. Positions 48-229.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-1100N.
IntActi P06974. 18 interactions.
MINTi MINT-1301311.
STRINGi 511145.b1945.

Proteomic databases

PaxDbi P06974.
PRIDEi P06974.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75012 ; AAC75012 ; b1945 .
BAA15770 ; BAA15770 ; BAA15770 .
GeneIDi 12932576.
946442.
KEGGi ecj:Y75_p1915.
eco:b1945.
PATRICi 32119219. VBIEscCol129921_2024.

Organism-specific databases

EchoBASEi EB0319.
EcoGenei EG10323. fliM.

Phylogenomic databases

eggNOGi COG1868.
HOGENOMi HOG000253580.
KOi K02416.
OMAi LFNMMRR.
OrthoDBi EOG68M4DH.
PhylomeDBi P06974.

Enzyme and pathway databases

BioCyci EcoCyc:FLIM-FLAGELLAR-C-RING-SWITCH.
ECOL316407:JW1929-MONOMER.

Miscellaneous databases

EvolutionaryTracei P06974.
PROi P06974.

Gene expression databases

Genevestigatori P06974.

Family and domain databases

InterProi IPR028976. CheC-like_dom.
IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view ]
Pfami PF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view ]
PIRSFi PIRSF002888. FliM. 1 hit.
PRINTSi PR00955. FLGMOTORFLIM.
SUPFAMi SSF101801. SSF101801. 1 hit.
SSF103039. SSF103039. 1 hit.
TIGRFAMsi TIGR01397. fliM_switch. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the flaA (cheC) locus of Escherichia coli and discovery of a new gene."
    Kuo S.C., Koshland D.E. Jr.
    J. Bacteriol. 166:1007-1012(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "DNA sequence analysis, gene product identification, and localization of flagellar motor components of Escherichia coli."
    Malakooti J., Komeda Y., Matsumura P.
    J. Bacteriol. 171:2728-2734(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 327-334.
    Strain: K12.
  6. "The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a 'backstop brake' mechanism."
    Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M.
    Mol. Cell 38:128-139(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YCRG, MUTAGENESIS OF VAL-153; ASN-155; ARG-156; LEU-160; ASP-167 AND GLU-176.
    Strain: K12 / RP3098.
  7. "Flagellar motility in bacteria structure and function of flagellar motor."
    Terashima H., Kojima S., Homma M.
    Int. Rev. Cytol. 270:39-85(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiFLIM_ECOLI
AccessioniPrimary (citable) accession number: P06974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: September 3, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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