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P06974 (FLIM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Flagellar motor switch protein FliM
Gene names
Name:fliM
Synonyms:cheC2, fla AII, fla QII
Ordered Locus Names:b1945, JW1929
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.

Subunit structure

Interacts with flagellar brake protein YcgR. Ref.6

Subcellular location

Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body.

Sequence similarities

Belongs to the FliM family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Flagellar motor switch protein FliM
PRO_0000180928

Experimental info

Mutagenesis1531V → D: Decreases binding to YcgR. Ref.6
Mutagenesis1551N → E: Decreases binding to YcgR. Ref.6
Mutagenesis1561R → D: Obviates binding to YcgR. Ref.6
Mutagenesis1601L → E: Obviates binding to YcgR. Ref.6
Mutagenesis1671D → R: Decreases binding to YcgR. Ref.6
Mutagenesis1761E → R: Decreases binding to YcgR. Ref.6

Secondary structure

... 334
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06974 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 05DC012B9364BFD6

FASTA33437,849
        10         20         30         40         50         60 
MGDSILSQAE IDALLNGDSE VKDEPTASVS GESDIRPYDP NTQRRVVRER LQALEIINER 

        70         80         90        100        110        120 
FARHFRMGLF NLLRRSPDIT VGAIRIQPYH EFARNLPVPT NLNLIHLKPL RGTGLVVFSP 

       130        140        150        160        170        180 
SLVFIAVDNL FGGDGRFPTK VEGREFTHTE QRVINRMLKL ALEGYSDAWK AINPLEVEYV 

       190        200        210        220        230        240 
RSEMQVKFTN ITTSPNDIVV NTPFHVEIGN LTGEFNICLP FSMIEPLREL LVNPPLENSR 

       250        260        270        280        290        300 
NEDQNWRDNL VRQVQHSQLE LVANFADISL RLSQILKLNP GDVLPIEKPD RIIAHVDGVP 

       310        320        330 
VLTSQYGTLN GQYALRIEHL INPILNSLNE EQPK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the flaA (cheC) locus of Escherichia coli and discovery of a new gene."
Kuo S.C., Koshland D.E. Jr.
J. Bacteriol. 166:1007-1012(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"DNA sequence analysis, gene product identification, and localization of flagellar motor components of Escherichia coli."
Malakooti J., Komeda Y., Matsumura P.
J. Bacteriol. 171:2728-2734(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 327-334.
Strain: K12.
[6]"The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a 'backstop brake' mechanism."
Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M.
Mol. Cell 38:128-139(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YCRG, MUTAGENESIS OF VAL-153; ASN-155; ARG-156; LEU-160; ASP-167 AND GLU-176.
Strain: K12 / RP3098.
[7]"Flagellar motility in bacteria structure and function of flagellar motor."
Terashima H., Kojima S., Homma M.
Int. Rev. Cytol. 270:39-85(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M12784 Genomic DNA. Translation: AAA23786.1.
U00096 Genomic DNA. Translation: AAC75012.1.
AP009048 Genomic DNA. Translation: BAA15770.1.
M26294 Unassigned DNA. No translation available.
PIRXMECF2. B29842.
RefSeqNP_416455.1. NC_000913.3.
YP_490199.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1F4VX-ray2.22D/E/F1-16[»]
1U8TX-ray1.50E/F1-16[»]
2B1JX-ray2.40C/D1-16[»]
ProteinModelPortalP06974.
SMRP06974. Positions 48-229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-1100N.
IntActP06974. 18 interactions.
MINTMINT-1301311.
STRING511145.b1945.

Proteomic databases

PaxDbP06974.
PRIDEP06974.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75012; AAC75012; b1945.
BAA15770; BAA15770; BAA15770.
GeneID12932576.
946442.
KEGGecj:Y75_p1915.
eco:b1945.
PATRIC32119219. VBIEscCol129921_2024.

Organism-specific databases

EchoBASEEB0319.
EcoGeneEG10323. fliM.

Phylogenomic databases

eggNOGCOG1868.
HOGENOMHOG000253580.
KOK02416.
OMALFNMMRR.
OrthoDBEOG68M4DH.
PhylomeDBP06974.

Enzyme and pathway databases

BioCycEcoCyc:FLIM-FLAGELLAR-C-RING-SWITCH.
ECOL316407:JW1929-MONOMER.

Gene expression databases

GenevestigatorP06974.

Family and domain databases

InterProIPR028976. CheC-like_dom.
IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]
PfamPF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view]
PIRSFPIRSF002888. FliM. 1 hit.
PRINTSPR00955. FLGMOTORFLIM.
SUPFAMSSF101801. SSF101801. 1 hit.
SSF103039. SSF103039. 1 hit.
TIGRFAMsTIGR01397. fliM_switch. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP06974.
PROP06974.

Entry information

Entry nameFLIM_ECOLI
AccessionPrimary (citable) accession number: P06974
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene