Flagellar motor switch protein FliM - Escherichia coli (strain K12)

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P06974 (FLIM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 121. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Flagellar motor switch protein FliM

Gene names

Name:	fliM
Synonyms:	cheC2, fla AII, fla QII
Ordered Locus Names:	b1945, JW1929

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.
Subunit structure	Interacts with flagellar brake protein YcgR. Ref.6
Subcellular location	Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body.
Sequence similarities	Belongs to the FliM family.

Ontologies

Keywords
Biological process	Chemotaxis Flagellar rotation
Cellular component	Bacterial flagellum Cell inner membrane Cell membrane Membrane
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW ciliary or flagellar motility Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	bacterial-type flagellum basal body Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
fliG	P0ABZ1	3	EBI-560439,EBI-1126524
fliN	P15070	3	EBI-560439,EBI-2011987
ycgR	P76010	3	EBI-560439,EBI-554507

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 334

334

Flagellar motor switch protein FliM

PRO_0000180928

Experimental info

Mutagenesis

153

V → D: Decreases binding to YcgR. Ref.6

Mutagenesis

155

N → E: Decreases binding to YcgR. Ref.6

Mutagenesis

156

R → D: Obviates binding to YcgR. Ref.6

Mutagenesis

160

L → E: Obviates binding to YcgR. Ref.6

Mutagenesis

167

D → R: Decreases binding to YcgR. Ref.6

Mutagenesis

176

E → R: Decreases binding to YcgR. Ref.6

Secondary structure

334

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P06974 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 05DC012B9364BFD6
Show »

FASTA

334

37,849

        10         20         30         40         50         60 
MGDSILSQAE IDALLNGDSE VKDEPTASVS GESDIRPYDP NTQRRVVRER LQALEIINER 

        70         80         90        100        110        120 
FARHFRMGLF NLLRRSPDIT VGAIRIQPYH EFARNLPVPT NLNLIHLKPL RGTGLVVFSP 

       130        140        150        160        170        180 
SLVFIAVDNL FGGDGRFPTK VEGREFTHTE QRVINRMLKL ALEGYSDAWK AINPLEVEYV 

       190        200        210        220        230        240 
RSEMQVKFTN ITTSPNDIVV NTPFHVEIGN LTGEFNICLP FSMIEPLREL LVNPPLENSR 

       250        260        270        280        290        300 
NEDQNWRDNL VRQVQHSQLE LVANFADISL RLSQILKLNP GDVLPIEKPD RIIAHVDGVP 

       310        320        330 
VLTSQYGTLN GQYALRIEHL INPILNSLNE EQPK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the flaA (cheC) locus of Escherichia coli and discovery of a new gene." Kuo S.C., Koshland D.E. Jr. J. Bacteriol. 166:1007-1012(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"DNA sequence analysis, gene product identification, and localization of flagellar motor components of Escherichia coli." Malakooti J., Komeda Y., Matsumura P. J. Bacteriol. 171:2728-2734(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 327-334. Strain: K12.
[6]	"The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a 'backstop brake' mechanism." Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M. Mol. Cell 38:128-139(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH YCRG, MUTAGENESIS OF VAL-153; ASN-155; ARG-156; LEU-160; ASP-167 AND GLU-176. Strain: K12 / RP3098.
[7]	"Flagellar motility in bacteria structure and function of flagellar motor." Terashima H., Kojima S., Homma M. Int. Rev. Cytol. 270:39-85(2008) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M12784 Genomic DNA. Translation: AAA23786.1.
U00096 Genomic DNA. Translation: AAC75012.1.
AP009048 Genomic DNA. Translation: BAA15770.1.
M26294 Unassigned DNA. No translation available.

PIR

XMECF2. B29842.

RefSeq

NP_416455.1. NC_000913.2.
YP_490199.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F4V	X-ray	2.22	D/E/F	1-16	[»]
1U8T	X-ray	1.50	E/F	1-16	[»]
2B1J	X-ray	2.40	C/D	1-16	[»]

ProteinModelPortal

P06974.

SMR

P06974. Positions 48-231.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1100N.

IntAct

P06974. 14 interactions.

MINT

MINT-1301311.

STRING

511145.b1945.

Proteomic databases

PaxDb

P06974.

PRIDE

P06974.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC75012; AAC75012; b1945.
BAA15770; BAA15770; BAA15770.

GeneID

12932576.
946442.

KEGG

ecj:Y75_p1915.
eco:b1945.

PATRIC

32119219. VBIEscCol129921_2024.

Organism-specific databases

EchoBASE

EB0319.

EcoGene

EG10323. fliM.

Phylogenomic databases

eggNOG

COG1868.

HOGENOM

HOG000253580.

K02416.

OMA

QTEQRII.

ProtClustDB

PRK06666.

Enzyme and pathway databases

BioCyc

EcoCyc:FLIM-FLAGELLAR-C-RING-SWITCH.
ECOL316407:JW1929-MONOMER.

Gene expression databases

Genevestigator

P06974.

Family and domain databases

InterPro

IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]

Pfam

PF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view]

PIRSF

PIRSF002888. FliM. 1 hit.

PRINTS

PR00955. FLGMOTORFLIM.

SUPFAM

SSF101801. SpoA. 1 hit.

TIGRFAMs

TIGR01397. fliM_switch. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P06974.

Entry information

Entry name

FLIM_ECOLI

Accession

Primary (citable) accession number: P06974

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents