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Protein

Flagellar motor switch protein FliM

Gene

fliM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.

GO - Molecular functioni

  1. motor activity Source: InterPro

GO - Biological processi

  1. bacterial-type flagellum-dependent cell motility Source: InterPro
  2. chemotaxis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCyciEcoCyc:FLIM-FLAGELLAR-C-RING-SWITCH.
ECOL316407:JW1929-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar motor switch protein FliM
Gene namesi
Name:fliM
Synonyms:cheC2, fla AII, fla QII
Ordered Locus Names:b1945, JW1929
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10323. fliM.

Subcellular locationi

GO - Cellular componenti

  1. bacterial-type flagellum basal body Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Bacterial flagellum, Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi153 – 1531V → D: Decreases binding to YcgR. 1 Publication
Mutagenesisi155 – 1551N → E: Decreases binding to YcgR. 1 Publication
Mutagenesisi156 – 1561R → D: Obviates binding to YcgR. 1 Publication
Mutagenesisi160 – 1601L → E: Obviates binding to YcgR. 1 Publication
Mutagenesisi167 – 1671D → R: Decreases binding to YcgR. 1 Publication
Mutagenesisi176 – 1761E → R: Decreases binding to YcgR. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Flagellar motor switch protein FliMPRO_0000180928Add
BLAST

Proteomic databases

PaxDbiP06974.
PRIDEiP06974.

Expressioni

Gene expression databases

GenevestigatoriP06974.

Interactioni

Subunit structurei

Interacts with flagellar brake protein YcgR.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
fliGP0ABZ116EBI-560439,EBI-1126524
fliNP150707EBI-560439,EBI-2011987
ycgRP760103EBI-560439,EBI-554507

Protein-protein interaction databases

DIPiDIP-1100N.
IntActiP06974. 18 interactions.
MINTiMINT-1301311.
STRINGi511145.b1945.

Structurei

Secondary structure

1
334
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 158Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F4VX-ray2.22D/E/F1-16[»]
1U8TX-ray1.50E/F1-16[»]
2B1JX-ray2.40C/D1-16[»]
ProteinModelPortaliP06974.
SMRiP06974. Positions 48-229.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06974.

Family & Domainsi

Sequence similaritiesi

Belongs to the FliM family.Curated

Phylogenomic databases

eggNOGiCOG1868.
HOGENOMiHOG000253580.
InParanoidiP06974.
KOiK02416.
OMAiNPQFTQI.
OrthoDBiEOG68M4DH.
PhylomeDBiP06974.

Family and domain databases

InterProiIPR028976. CheC-like_dom.
IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]
PfamiPF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view]
PIRSFiPIRSF002888. FliM. 1 hit.
PRINTSiPR00955. FLGMOTORFLIM.
SUPFAMiSSF101801. SSF101801. 1 hit.
SSF103039. SSF103039. 1 hit.
TIGRFAMsiTIGR01397. fliM_switch. 1 hit.

Sequencei

Sequence statusi: Complete.

P06974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSILSQAE IDALLNGDSE VKDEPTASVS GESDIRPYDP NTQRRVVRER
60 70 80 90 100
LQALEIINER FARHFRMGLF NLLRRSPDIT VGAIRIQPYH EFARNLPVPT
110 120 130 140 150
NLNLIHLKPL RGTGLVVFSP SLVFIAVDNL FGGDGRFPTK VEGREFTHTE
160 170 180 190 200
QRVINRMLKL ALEGYSDAWK AINPLEVEYV RSEMQVKFTN ITTSPNDIVV
210 220 230 240 250
NTPFHVEIGN LTGEFNICLP FSMIEPLREL LVNPPLENSR NEDQNWRDNL
260 270 280 290 300
VRQVQHSQLE LVANFADISL RLSQILKLNP GDVLPIEKPD RIIAHVDGVP
310 320 330
VLTSQYGTLN GQYALRIEHL INPILNSLNE EQPK
Length:334
Mass (Da):37,849
Last modified:April 1, 1988 - v1
Checksum:i05DC012B9364BFD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12784 Genomic DNA. Translation: AAA23786.1.
U00096 Genomic DNA. Translation: AAC75012.1.
AP009048 Genomic DNA. Translation: BAA15770.1.
M26294 Unassigned DNA. No translation available.
PIRiB29842. XMECF2.
RefSeqiNP_416455.1. NC_000913.3.
YP_490199.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75012; AAC75012; b1945.
BAA15770; BAA15770; BAA15770.
GeneIDi12932576.
946442.
KEGGiecj:Y75_p1915.
eco:b1945.
PATRICi32119219. VBIEscCol129921_2024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12784 Genomic DNA. Translation: AAA23786.1.
U00096 Genomic DNA. Translation: AAC75012.1.
AP009048 Genomic DNA. Translation: BAA15770.1.
M26294 Unassigned DNA. No translation available.
PIRiB29842. XMECF2.
RefSeqiNP_416455.1. NC_000913.3.
YP_490199.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F4VX-ray2.22D/E/F1-16[»]
1U8TX-ray1.50E/F1-16[»]
2B1JX-ray2.40C/D1-16[»]
ProteinModelPortaliP06974.
SMRiP06974. Positions 48-229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-1100N.
IntActiP06974. 18 interactions.
MINTiMINT-1301311.
STRINGi511145.b1945.

Proteomic databases

PaxDbiP06974.
PRIDEiP06974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75012; AAC75012; b1945.
BAA15770; BAA15770; BAA15770.
GeneIDi12932576.
946442.
KEGGiecj:Y75_p1915.
eco:b1945.
PATRICi32119219. VBIEscCol129921_2024.

Organism-specific databases

EchoBASEiEB0319.
EcoGeneiEG10323. fliM.

Phylogenomic databases

eggNOGiCOG1868.
HOGENOMiHOG000253580.
InParanoidiP06974.
KOiK02416.
OMAiNPQFTQI.
OrthoDBiEOG68M4DH.
PhylomeDBiP06974.

Enzyme and pathway databases

BioCyciEcoCyc:FLIM-FLAGELLAR-C-RING-SWITCH.
ECOL316407:JW1929-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06974.
PROiP06974.

Gene expression databases

GenevestigatoriP06974.

Family and domain databases

InterProiIPR028976. CheC-like_dom.
IPR001689. Flag_FliM.
IPR001543. SpoA.
[Graphical view]
PfamiPF02154. FliM. 1 hit.
PF01052. SpoA. 1 hit.
[Graphical view]
PIRSFiPIRSF002888. FliM. 1 hit.
PRINTSiPR00955. FLGMOTORFLIM.
SUPFAMiSSF101801. SSF101801. 1 hit.
SSF103039. SSF103039. 1 hit.
TIGRFAMsiTIGR01397. fliM_switch. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the flaA (cheC) locus of Escherichia coli and discovery of a new gene."
    Kuo S.C., Koshland D.E. Jr.
    J. Bacteriol. 166:1007-1012(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "DNA sequence analysis, gene product identification, and localization of flagellar motor components of Escherichia coli."
    Malakooti J., Komeda Y., Matsumura P.
    J. Bacteriol. 171:2728-2734(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 327-334.
    Strain: K12.
  6. "The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a 'backstop brake' mechanism."
    Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M.
    Mol. Cell 38:128-139(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YCRG, MUTAGENESIS OF VAL-153; ASN-155; ARG-156; LEU-160; ASP-167 AND GLU-176.
    Strain: K12 / RP3098.
  7. "Flagellar motility in bacteria structure and function of flagellar motor."
    Terashima H., Kojima S., Homma M.
    Int. Rev. Cytol. 270:39-85(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiFLIM_ECOLI
AccessioniPrimary (citable) accession number: P06974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: January 7, 2015
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.