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Protein

Iron(3+)-hydroxamate import system permease protein FhuB

Gene

fhuB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex FhuCDB involved in iron3+-hydroxamate import. Responsible for the translocation of the substrate across the membrane.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processIon transport, Iron transport, Transport
LigandIron

Enzyme and pathway databases

BioCyciEcoCyc:FHUB-MONOMER.
MetaCyc:FHUB-MONOMER.

Protein family/group databases

TCDBi3.A.1.14.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron(3+)-hydroxamate import system permease protein FhuBCurated
Alternative name(s):
Ferric hydroxamate uptake protein BCurated
Ferrichrome transport system permease protein FhuBCurated
Ferrichrome uptake protein FhuBCurated
Iron(III)-hydroxamate import system permease protein FhuBCurated
Gene namesi
Name:fhuB
Ordered Locus Names:b0153, JW0149
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10303. fhuB.

Subcellular locationi

P06972:

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei5 – 25HelicalSequence analysisAdd BLAST21
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
Transmembranei277 – 297HelicalSequence analysisAdd BLAST21
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Transmembranei348 – 368HelicalSequence analysisAdd BLAST21
Transmembranei391 – 411HelicalSequence analysisAdd BLAST21
Transmembranei424 – 444HelicalSequence analysisAdd BLAST21
Transmembranei447 – 467HelicalSequence analysisAdd BLAST21
Transmembranei479 – 499HelicalSequence analysisAdd BLAST21
Transmembranei528 – 548HelicalSequence analysisAdd BLAST21
Transmembranei567 – 587HelicalSequence analysisAdd BLAST21
Transmembranei607 – 627HelicalSequence analysisAdd BLAST21
Transmembranei635 – 655HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000600281 – 660Iron(3+)-hydroxamate import system permease protein FhuBAdd BLAST660

Proteomic databases

PaxDbiP06972.
PRIDEiP06972.

Expressioni

Inductioni

Induced 2.1-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (FhuC), two transmembrane proteins (FhuB) and a solute-binding protein (FhuD) (Probable). FhuB interacts with FhuC and FhuD (PubMed:1551849, PubMed:8522527, PubMed:9426146).Curated3 Publications

Protein-protein interaction databases

BioGridi4259735. 143 interactors.
DIPiDIP-9603N.
IntActiP06972. 1 interactor.
MINTiMINT-1304500.
STRINGi316385.ECDH10B_0133.

Structurei

3D structure databases

ProteinModelPortaliP06972.
SMRiP06972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

FhuD interacts with a periplasmic and a transmembrane/cytoplasmic region of FhuB. The transmembrane region may be part of a pore through which a portion of FhuD inserts into the cytoplasmic membrane during transport.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105S6R. Bacteria.
COG0609. LUCA.
HOGENOMiHOG000260186.
InParanoidiP06972.
KOiK02015.

Family and domain databases

Gene3Di1.10.3470.10. 2 hits.
InterProiView protein in InterPro
IPR035568. ABC_transport_FecCD/TroCD-like.
IPR000522. ABC_transptr_FecCD.
PANTHERiPTHR30472. PTHR30472. 3 hits.
PfamiView protein in Pfam
PF01032. FecCD. 2 hits.
SUPFAMiSSF81345. SSF81345. 2 hits.

Sequencei

Sequence statusi: Complete.

P06972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRIALFPA LLLALLVIVA TALTWMNFSQ ALPRSQWAQA AWSPDIDVIE
60 70 80 90 100
QMIFHYSLLP RLAISLLVGA GLGLVGVLFQ QVLRNPLAEP TTLGVATGAQ
110 120 130 140 150
LGITVTTLWA IPGAMASQFA AQAGACVVGL IVFGVAWGKR LSPVTLILAG
160 170 180 190 200
LVVSLYCGAI NQLLVIFHHD QLQSMFLWST GTLTQTDWGG VERLWPQLLG
210 220 230 240 250
GVMLTLLLLR PLTLMGLDDG VARNLGLALS LARLAALSLA IVISALLVNA
260 270 280 290 300
VGIIGFIGLF APLLAKMLGA RRLLPRLMLA SLIGALILWL SDQIILWLTR
310 320 330 340 350
VWMEVSTGSV TALIGAPLLL WLLPRLRSIS APDMKVNDRV AAERQHVLAF
360 370 380 390 400
ALAGGVLLLM AVVVALSFGR DAHGWTWASG ALLEDLMPWR WPRIMAALFA
410 420 430 440 450
GVMLAVAGCI IQRLTGNPMA SPEVLGISSG AAFGVVLMLF LVPGNAFGWL
460 470 480 490 500
LPAGSLGAAV TLLIIMIAAG RGGFSPHRML LAGMALSTAF TMLLMMLQAS
510 520 530 540 550
GDPRMAQVLT WISGSTYNAT DAQVWRTGIV MVILLAITPL CRRWLTILPL
560 570 580 590 600
GGDTARAVGM ALTPTRIALL LLAACLTATA TMTIGPLSFV GLMAPHIARM
610 620 630 640 650
MGFRRTMPHI VISALVGGLL LVFADWCGRM VLFPFQIPAG LLSTFIGAPY
660
FIYLLRKQSR
Length:660
Mass (Da):70,423
Last modified:November 1, 1997 - v2
Checksum:i427313E7B421B0BC
GO

Sequence cautioni

P06972: The sequence CAA27853 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11L → V in CAA29256 (PubMed:2823072).Curated1
Sequence conflicti311T → I in CAA27852 (PubMed:3020380).Curated1
Sequence conflicti311T → I in CAA27853 (PubMed:3020380).Curated1
Sequence conflicti364Missing in CAA27852 (PubMed:3020380).Curated1
Sequence conflicti364Missing in CAA27853 (PubMed:3020380).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04319 Genomic DNA. Translation: CAA27852.1.
X04319 Genomic DNA. Translation: CAA27853.1. Different initiation.
U70214 Genomic DNA. Translation: AAB08583.1.
U00096 Genomic DNA. Translation: AAC73264.1.
AP009048 Genomic DNA. Translation: BAB96729.2.
X05810 Genomic DNA. Translation: CAA29256.1.
PIRiA64739.
RefSeqiNP_414695.1. NC_000913.3.
WP_000044072.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73264; AAC73264; b0153.
BAB96729; BAB96729; BAB96729.
GeneIDi946890.
KEGGiecj:JW0149.
eco:b0153.
PATRICifig|1411691.4.peg.2127.

Similar proteinsi

Entry informationi

Entry nameiFHUB_ECOLI
AccessioniPrimary (citable) accession number: P06972
Secondary accession number(s): P77372
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: October 25, 2017
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families