Reviewed, UniProtKB/Swiss-Prot P06972 (FHUB_ECOLI)

Names and origin

Protein names

Recommended name:
    Iron(3+)-hydroxamate import system permease protein fhuB
Alternative name(s):
    Iron(III)-hydroxamate import system permease protein fhuB
    Ferrichrome transport system permease protein fhuB
    Ferrichrome uptake protein fhuB
    Ferric hydroxamate uptake protein B

Gene names

Name:	fhuB
Ordered Locus Names:	b0153, JW0149

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of the ABC transporter complex fhuCDB involved in iron3+-hydroxamate import. Responsible for the translocation of the substrate across the membrane. Ref.7
Subunit structure	The complex is composed of an ATP-binding protein (fhuC), two transmembrane proteins (fhuB) and a solute-binding protein (fhuD).
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Ref.8
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	Iron
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	iron ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Iron(3+)-hydroxamate import system permease protein fhuB		PRO_0000060028
Regions
	Transmembrane	5 – 25	21	Potential
	Transmembrane	63 – 83	21	Potential
	Transmembrane	93 – 113	21	Potential
	Transmembrane	118 – 138	21	Potential
	Transmembrane	147 – 167	21	Potential
	Transmembrane	240 – 260	21	Potential
	Transmembrane	277 – 297	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	348 – 368	21	Potential
	Transmembrane	391 – 411	21	Potential
	Transmembrane	424 – 444	21	Potential
	Transmembrane	447 – 467	21	Potential
	Transmembrane	479 – 499	21	Potential
	Transmembrane	528 – 548	21	Potential
	Transmembrane	567 – 587	21	Potential
	Transmembrane	607 – 627	21	Potential
	Transmembrane	635 – 655	21	Potential
Experimental info
	Sequence conflict	11	1	L → V in CAA29256. Ref.6
	Sequence conflict	311	1	T → I Ref.1
	Sequence conflict	311	1	T → I Ref.2
	Sequence conflict	364	1	Missing Ref.1
	Sequence conflict	364	1	Missing Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P06972-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 427313E7B421B0BC	FASTA	660	70,423
	10 20 30 40 50 60 MSKRIALFPA LLLALLVIVA TALTWMNFSQ ALPRSQWAQA AWSPDIDVIE QMIFHYSLLP 70 80 90 100 110 120 RLAISLLVGA GLGLVGVLFQ QVLRNPLAEP TTLGVATGAQ LGITVTTLWA IPGAMASQFA 130 140 150 160 170 180 AQAGACVVGL IVFGVAWGKR LSPVTLILAG LVVSLYCGAI NQLLVIFHHD QLQSMFLWST 190 200 210 220 230 240 GTLTQTDWGG VERLWPQLLG GVMLTLLLLR PLTLMGLDDG VARNLGLALS LARLAALSLA 250 260 270 280 290 300 IVISALLVNA VGIIGFIGLF APLLAKMLGA RRLLPRLMLA SLIGALILWL SDQIILWLTR 310 320 330 340 350 360 VWMEVSTGSV TALIGAPLLL WLLPRLRSIS APDMKVNDRV AAERQHVLAF ALAGGVLLLM 370 380 390 400 410 420 AVVVALSFGR DAHGWTWASG ALLEDLMPWR WPRIMAALFA GVMLAVAGCI IQRLTGNPMA 430 440 450 460 470 480 SPEVLGISSG AAFGVVLMLF LVPGNAFGWL LPAGSLGAAV TLLIIMIAAG RGGFSPHRML 490 500 510 520 530 540 LAGMALSTAF TMLLMMLQAS GDPRMAQVLT WISGSTYNAT DAQVWRTGIV MVILLAITPL 550 560 570 580 590 600 CRRWLTILPL GGDTARAVGM ALTPTRIALL LLAACLTATA TMTIGPLSFV GLMAPHIARM 610 620 630 640 650 660 MGFRRTMPHI VISALVGGLL LVFADWCGRM VLFPFQIPAG LLSTFIGAPY FIYLLRKQSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Iron hydroxamate transport of Escherichia coli: nucleotide sequence of the fhuB gene and identification of the protein." Koester W., Braun V. Mol. Gen. Genet. 204:435-442(1986) [PubMed: 3020380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 311 AND 364. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Nucleotide sequence of the fhuC and fhuD genes involved in iron (III) hydroxamate transport: domains in FhuC homologous to ATP-binding proteins." Burkhardt R., Braun V. Mol. Gen. Genet. 209:49-55(1987) [PubMed: 2823072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
[7]	"Iron(III) hydroxamate transport in Escherichia coli K-12: FhuB-mediated membrane association of the FhuC protein and negative complementation of fhuC mutants." Schultz-Hauser G., Koester W., Schwarz H., Braun V. J. Bacteriol. 174:2305-2311(1992) [PubMed: 1551849] [Abstract] Cited for: FUNCTION IN IRON(3+)-HYDROXAMATE TRANSPORT, INTERACTION WITH FHUC. Strain: K12.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04319 Genomic DNA. Translation: CAA27852.1. X04319 Genomic DNA. Translation: CAA27853.1. Different initiation. U70214 Genomic DNA. Translation: AAB08583.1. U00096 Genomic DNA. Translation: AAC73264.1. AP009048 Genomic DNA. Translation: BAB96729.2. X05810 Genomic DNA. Translation: CAA29256.1.
PIR	A64739.
RefSeq	AP_000814.1. NP_414695.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9603N.
Protein family/group databases
TCDB	3.A.1.14.3. ATP-binding cassette (ABC) superfamily.
Genome annotation databases
GeneID	946890.
GenomeReviews	Gene locus JW0149 in contig AP009048_GR. Gene locus b0153 in contig U00096_GR.
KEGG	ecj:JW0149. eco:b0153.
Organism-specific databases
EchoBASE	EB0299.
EcoGene	EG10303. fhuB.
CMR	Search...
Phylogenomic databases
HOGENOM	P06972.
OMA	P06972. LFVAACW.
Enzyme and pathway databases
BioCyc	EcoCyc:FHUB-MON.
Family and domain databases
InterPro	IPR000522. FecD. [Graphical view]
Pfam	PF01032. FecCD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FHUB_ECOLI

Accession

Primary (citable) accession number: P06972
Secondary accession number(s): P77372

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)