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Protein

Ferrichrome-iron receptor

Gene

fhuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This receptor binds the ferrichrome-iron ligand. It interacts with the TonB protein, which is responsible for energy coupling of the ferrichrome-promoted iron transport system. Acts as a receptor for bacteriophage T5 as well as T1, phi80 and colicin M. Binding of T5 triggers the opening of a high conductance ion channel. Can also transport the antibiotic albomycin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114Ferrichrome1
Binding sitei133Ferrichrome1
Binding sitei277Ferrichrome1
Binding sitei279Ferrichrome1
Binding sitei346Ferrichrome1
Binding sitei348Ferrichrome1
Binding sitei424Ferrichrome1
Binding sitei735Ferrichrome1

GO - Molecular functioni

  • cofactor binding Source: EcoliWiki
  • iron ion binding Source: EcoliWiki
  • receptor activity Source: InterPro
  • siderophore uptake transmembrane transporter activity Source: GO_Central
  • toxic substance binding Source: EcoliWiki
  • virion binding Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:EG10302-MONOMER.
ECOL316407:JW0146-MONOMER.
MetaCyc:EG10302-MONOMER.

Protein family/group databases

TCDBi1.B.14.1.2. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrichrome-iron receptor
Alternative name(s):
Ferric hydroxamate receptor
Ferric hydroxamate uptake
Gene namesi
Name:fhuA
Synonyms:tonA
Ordered Locus Names:b0150, JW0146
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10302. fhuA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 192PeriplasmicAdd BLAST159
Transmembranei193 – 201Beta stranded9
Topological domaini202 – 206Extracellular5
Transmembranei207 – 215Beta stranded9
Topological domaini216 – 222Periplasmic7
Transmembranei223 – 231Beta stranded9
Topological domaini232 – 245ExtracellularAdd BLAST14
Transmembranei246 – 255Beta stranded10
Topological domaini256 – 259Periplasmic4
Transmembranei260 – 268Beta stranded9
Topological domaini269 – 312ExtracellularAdd BLAST44
Transmembranei313 – 321Beta stranded9
Topological domaini322 – 326Periplasmic5
Transmembranei327 – 335Beta stranded9
Topological domaini336 – 387ExtracellularAdd BLAST52
Transmembranei388 – 396Beta stranded9
Topological domaini397 – 404Periplasmic8
Transmembranei405 – 413Beta stranded9
Topological domaini414 – 464ExtracellularAdd BLAST51
Transmembranei465 – 473Beta stranded9
Topological domaini474 – 477Periplasmic4
Transmembranei478 – 486Beta stranded9
Topological domaini487 – 508ExtracellularAdd BLAST22
Transmembranei509 – 517Beta stranded9
Topological domaini518 – 522Periplasmic5
Transmembranei523 – 531Beta stranded9
Topological domaini532 – 551ExtracellularAdd BLAST20
Transmembranei552 – 560Beta stranded9
Topological domaini561 – 565Periplasmic5
Transmembranei566 – 574Beta stranded9
Topological domaini575 – 601ExtracellularAdd BLAST27
Transmembranei602 – 610Beta stranded9
Topological domaini611 – 613Periplasmic3
Transmembranei614 – 622Beta stranded9
Topological domaini623 – 645ExtracellularAdd BLAST23
Transmembranei646 – 654Beta stranded9
Topological domaini655 – 661Periplasmic7
Transmembranei662 – 670Beta stranded9
Topological domaini671 – 689ExtracellularAdd BLAST19
Transmembranei690 – 698Beta stranded9
Topological domaini699 – 705Periplasmic7
Transmembranei706 – 714Beta stranded9
Topological domaini715 – 737ExtracellularAdd BLAST23
Transmembranei738 – 746Beta stranded9
Topological domaini747Periplasmic1

GO - Cellular componenti

  • cell outer membrane Source: CACAO
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000003474834 – 747Ferrichrome-iron receptorAdd BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi351 ↔ 362
Disulfide bondi725 ↔ 731

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP06971.
PaxDbiP06971.
PRIDEiP06971.

Expressioni

Inductioni

Induced 1.6-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4259745. 124 interactors.
DIPiDIP-9602N.
IntActiP06971. 5 interactors.
STRINGi511145.b0150.

Structurei

Secondary structure

1747
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi44 – 46Combined sources3
Beta strandi54 – 56Combined sources3
Helixi57 – 62Combined sources6
Beta strandi65 – 67Combined sources3
Turni68 – 70Combined sources3
Helixi76 – 78Combined sources3
Beta strandi83 – 87Combined sources5
Helixi88 – 94Combined sources7
Helixi99 – 102Combined sources4
Turni103 – 105Combined sources3
Beta strandi106 – 111Combined sources6
Turni113 – 116Combined sources4
Beta strandi124 – 127Combined sources4
Helixi131 – 133Combined sources3
Beta strandi137 – 139Combined sources3
Helixi156 – 158Combined sources3
Beta strandi159 – 167Combined sources9
Helixi170 – 173Combined sources4
Beta strandi180 – 186Combined sources7
Beta strandi194 – 202Combined sources9
Helixi203 – 205Combined sources3
Beta strandi207 – 218Combined sources12
Beta strandi222 – 235Combined sources14
Beta strandi237 – 240Combined sources4
Beta strandi242 – 254Combined sources13
Beta strandi260 – 272Combined sources13
Turni284 – 286Combined sources3
Beta strandi307 – 322Combined sources16
Beta strandi324 – 350Combined sources27
Helixi354 – 356Combined sources3
Helixi360 – 363Combined sources4
Turni367 – 371Combined sources5
Beta strandi372 – 400Combined sources29
Beta strandi403 – 435Combined sources33
Turni436 – 438Combined sources3
Beta strandi445 – 447Combined sources3
Turni450 – 452Combined sources3
Beta strandi453 – 475Combined sources23
Beta strandi478 – 495Combined sources18
Turni496 – 499Combined sources4
Beta strandi500 – 517Combined sources18
Turni520 – 522Combined sources3
Beta strandi523 – 534Combined sources12
Beta strandi543 – 545Combined sources3
Beta strandi549 – 560Combined sources12
Beta strandi563 – 584Combined sources22
Beta strandi586 – 588Combined sources3
Beta strandi590 – 612Combined sources23
Beta strandi615 – 630Combined sources16
Turni632 – 636Combined sources5
Beta strandi644 – 655Combined sources12
Beta strandi656 – 658Combined sources3
Turni659 – 662Combined sources4
Beta strandi663 – 672Combined sources10
Beta strandi675 – 678Combined sources4
Beta strandi683 – 685Combined sources3
Beta strandi688 – 697Combined sources10
Helixi699 – 702Combined sources4
Beta strandi708 – 715Combined sources8
Beta strandi722 – 727Combined sources6
Beta strandi730 – 733Combined sources4
Beta strandi738 – 747Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BY3X-ray2.74A34-747[»]
1BY5X-ray2.60A34-747[»]
1FCPX-ray2.70A52-747[»]
1FI1X-ray2.90A52-747[»]
1QFFX-ray2.70A34-747[»]
1QFGX-ray2.50A34-747[»]
1QJQX-ray2.95A34-747[»]
1QKCX-ray3.10A34-747[»]
2FCPX-ray2.50A34-747[»]
2GRXX-ray3.30A/B34-747[»]
ProteinModelPortaliP06971.
SMRiP06971.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06971.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 149Ferrichrome binding2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 47TonB box8
Motifi730 – 747TonB C-terminal boxAdd BLAST18

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105E0K. Bacteria.
COG1629. LUCA.
HOGENOMiHOG000260252.
InParanoidiP06971.
KOiK02014.
OMAiTVRENSY.
PhylomeDBiP06971.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSKTAQPK HSLRKIAVVV ATAVSGMSVY AQAAVEPKED TITVTAAPAP
60 70 80 90 100
QESAWGPAAT IAARQSATGT KTDTPIQKVP QSISVVTAEE MALHQPKSVK
110 120 130 140 150
EALSYTPGVS VGTRGASNTY DHLIIRGFAA EGQSQNNYLN GLKLQGNFYN
160 170 180 190 200
DAVIDPYMLE RAEIMRGPVS VLYGKSSPGG LLNMVSKRPT TEPLKEVQFK
210 220 230 240 250
AGTDSLFQTG FDFSDSLDDD GVYSYRLTGL ARSANAQQKG SEEQRYAIAP
260 270 280 290 300
AFTWRPDDKT NFTFLSYFQN EPETGYYGWL PKEGTVEPLP NGKRLPTDFN
310 320 330 340 350
EGAKNNTYSR NEKMVGYSFD HEFNDTFTVR QNLRFAENKT SQNSVYGYGV
360 370 380 390 400
CSDPANAYSK QCAALAPADK GHYLARKYVV DDEKLQNFSV DTQLQSKFAT
410 420 430 440 450
GDIDHTLLTG VDFMRMRNDI NAWFGYDDSV PLLNLYNPVN TDFDFNAKDP
460 470 480 490 500
ANSGPYRILN KQKQTGVYVQ DQAQWDKVLV TLGGRYDWAD QESLNRVAGT
510 520 530 540 550
TDKRDDKQFT WRGGVNYLFD NGVTPYFSYS ESFEPSSQVG KDGNIFAPSK
560 570 580 590 600
GKQYEVGVKY VPEDRPIVVT GAVYNLTKTN NLMADPEGSF FSVEGGEIRA
610 620 630 640 650
RGVEIEAKAA LSASVNVVGS YTYTDAEYTT DTTYKGNTPA QVPKHMASLW
660 670 680 690 700
ADYTFFDGPL SGLTLGTGGR YTGSSYGDPA NSFKVGSYTV VDALVRYDLA
710 720 730 740
RVGMAGSNVA LHVNNLFDRE YVASCFNTYG CFWGAERQVV ATATFRF
Length:747
Mass (Da):82,182
Last modified:November 1, 1997 - v2
Checksum:i1C2B251D1990E444
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti737R → P in CAA29253 (PubMed:2823072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12486 Genomic DNA. Translation: AAB61768.1.
U00096 Genomic DNA. Translation: AAC73261.1.
AP009048 Genomic DNA. Translation: BAB96726.2.
U70214 Genomic DNA. Translation: AAB08580.1.
X05810 Genomic DNA. Translation: CAA29253.1.
PIRiF64738. QRECFE.
RefSeqiNP_414692.1. NC_000913.3.
WP_000124438.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73261; AAC73261; b0150.
BAB96726; BAB96726; BAB96726.
GeneIDi944856.
KEGGiecj:JW0146.
eco:b0150.
PATRICi32115409. VBIEscCol129921_0156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12486 Genomic DNA. Translation: AAB61768.1.
U00096 Genomic DNA. Translation: AAC73261.1.
AP009048 Genomic DNA. Translation: BAB96726.2.
U70214 Genomic DNA. Translation: AAB08580.1.
X05810 Genomic DNA. Translation: CAA29253.1.
PIRiF64738. QRECFE.
RefSeqiNP_414692.1. NC_000913.3.
WP_000124438.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BY3X-ray2.74A34-747[»]
1BY5X-ray2.60A34-747[»]
1FCPX-ray2.70A52-747[»]
1FI1X-ray2.90A52-747[»]
1QFFX-ray2.70A34-747[»]
1QFGX-ray2.50A34-747[»]
1QJQX-ray2.95A34-747[»]
1QKCX-ray3.10A34-747[»]
2FCPX-ray2.50A34-747[»]
2GRXX-ray3.30A/B34-747[»]
ProteinModelPortaliP06971.
SMRiP06971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259745. 124 interactors.
DIPiDIP-9602N.
IntActiP06971. 5 interactors.
STRINGi511145.b0150.

Protein family/group databases

TCDBi1.B.14.1.2. the outer membrane receptor (omr) family.

Proteomic databases

EPDiP06971.
PaxDbiP06971.
PRIDEiP06971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73261; AAC73261; b0150.
BAB96726; BAB96726; BAB96726.
GeneIDi944856.
KEGGiecj:JW0146.
eco:b0150.
PATRICi32115409. VBIEscCol129921_0156.

Organism-specific databases

EchoBASEiEB0298.
EcoGeneiEG10302. fhuA.

Phylogenomic databases

eggNOGiENOG4105E0K. Bacteria.
COG1629. LUCA.
HOGENOMiHOG000260252.
InParanoidiP06971.
KOiK02014.
OMAiTVRENSY.
PhylomeDBiP06971.

Enzyme and pathway databases

BioCyciEcoCyc:EG10302-MONOMER.
ECOL316407:JW0146-MONOMER.
MetaCyc:EG10302-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06971.
PROiP06971.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFHUA_ECOLI
AccessioniPrimary (citable) accession number: P06971
Secondary accession number(s): P71280, P75665
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 174 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.