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Protein

Ferrichrome-iron receptor

Gene

fhuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This receptor binds the ferrichrome-iron ligand. It interacts with the TonB protein, which is responsible for energy coupling of the ferrichrome-promoted iron transport system. Acts as a receptor for bacteriophage T5 as well as T1, phi80 and colicin M. Binding of T5 triggers the opening of a high conductance ion channel. Can also transport the antibiotic albomycin.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei114 – 1141Ferrichrome
Binding sitei133 – 1331Ferrichrome
Binding sitei277 – 2771Ferrichrome
Binding sitei279 – 2791Ferrichrome
Binding sitei346 – 3461Ferrichrome
Binding sitei348 – 3481Ferrichrome
Binding sitei424 – 4241Ferrichrome
Binding sitei735 – 7351Ferrichrome

GO - Molecular functioni

  • cofactor binding Source: EcoliWiki
  • iron ion binding Source: EcoliWiki
  • receptor activity Source: InterPro
  • siderophore uptake transmembrane transporter activity Source: GO_Central
  • toxic substance binding Source: EcoliWiki
  • virion binding Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:EG10302-MONOMER.
ECOL316407:JW0146-MONOMER.
MetaCyc:EG10302-MONOMER.

Protein family/group databases

TCDBi1.B.14.1.2. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrichrome-iron receptor
Alternative name(s):
Ferric hydroxamate receptor
Ferric hydroxamate uptake
Gene namesi
Name:fhuA
Synonyms:tonA
Ordered Locus Names:b0150, JW0146
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10302. fhuA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 192159PeriplasmicAdd
BLAST
Transmembranei193 – 2019Beta stranded
Topological domaini202 – 2065Extracellular
Transmembranei207 – 2159Beta stranded
Topological domaini216 – 2227Periplasmic
Transmembranei223 – 2319Beta stranded
Topological domaini232 – 24514ExtracellularAdd
BLAST
Transmembranei246 – 25510Beta stranded
Topological domaini256 – 2594Periplasmic
Transmembranei260 – 2689Beta stranded
Topological domaini269 – 31244ExtracellularAdd
BLAST
Transmembranei313 – 3219Beta stranded
Topological domaini322 – 3265Periplasmic
Transmembranei327 – 3359Beta stranded
Topological domaini336 – 38752ExtracellularAdd
BLAST
Transmembranei388 – 3969Beta stranded
Topological domaini397 – 4048Periplasmic
Transmembranei405 – 4139Beta stranded
Topological domaini414 – 46451ExtracellularAdd
BLAST
Transmembranei465 – 4739Beta stranded
Topological domaini474 – 4774Periplasmic
Transmembranei478 – 4869Beta stranded
Topological domaini487 – 50822ExtracellularAdd
BLAST
Transmembranei509 – 5179Beta stranded
Topological domaini518 – 5225Periplasmic
Transmembranei523 – 5319Beta stranded
Topological domaini532 – 55120ExtracellularAdd
BLAST
Transmembranei552 – 5609Beta stranded
Topological domaini561 – 5655Periplasmic
Transmembranei566 – 5749Beta stranded
Topological domaini575 – 60127ExtracellularAdd
BLAST
Transmembranei602 – 6109Beta stranded
Topological domaini611 – 6133Periplasmic
Transmembranei614 – 6229Beta stranded
Topological domaini623 – 64523ExtracellularAdd
BLAST
Transmembranei646 – 6549Beta stranded
Topological domaini655 – 6617Periplasmic
Transmembranei662 – 6709Beta stranded
Topological domaini671 – 68919ExtracellularAdd
BLAST
Transmembranei690 – 6989Beta stranded
Topological domaini699 – 7057Periplasmic
Transmembranei706 – 7149Beta stranded
Topological domaini715 – 73723ExtracellularAdd
BLAST
Transmembranei738 – 7469Beta stranded
Topological domaini747 – 7471Periplasmic

GO - Cellular componenti

  • cell outer membrane Source: CACAO
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 33331 PublicationAdd
BLAST
Chaini34 – 747714Ferrichrome-iron receptorPRO_0000034748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi351 ↔ 362
Disulfide bondi725 ↔ 731

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP06971.
PaxDbiP06971.
PRIDEiP06971.

Expressioni

Inductioni

Induced 1.6-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4259745. 124 interactions.
DIPiDIP-9602N.
IntActiP06971. 5 interactions.
STRINGi511145.b0150.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi44 – 463Combined sources
Beta strandi54 – 563Combined sources
Helixi57 – 626Combined sources
Beta strandi65 – 673Combined sources
Turni68 – 703Combined sources
Helixi76 – 783Combined sources
Beta strandi83 – 875Combined sources
Helixi88 – 947Combined sources
Helixi99 – 1024Combined sources
Turni103 – 1053Combined sources
Beta strandi106 – 1116Combined sources
Turni113 – 1164Combined sources
Beta strandi124 – 1274Combined sources
Helixi131 – 1333Combined sources
Beta strandi137 – 1393Combined sources
Helixi156 – 1583Combined sources
Beta strandi159 – 1679Combined sources
Helixi170 – 1734Combined sources
Beta strandi180 – 1867Combined sources
Beta strandi194 – 2029Combined sources
Helixi203 – 2053Combined sources
Beta strandi207 – 21812Combined sources
Beta strandi222 – 23514Combined sources
Beta strandi237 – 2404Combined sources
Beta strandi242 – 25413Combined sources
Beta strandi260 – 27213Combined sources
Turni284 – 2863Combined sources
Beta strandi307 – 32216Combined sources
Beta strandi324 – 35027Combined sources
Helixi354 – 3563Combined sources
Helixi360 – 3634Combined sources
Turni367 – 3715Combined sources
Beta strandi372 – 40029Combined sources
Beta strandi403 – 43533Combined sources
Turni436 – 4383Combined sources
Beta strandi445 – 4473Combined sources
Turni450 – 4523Combined sources
Beta strandi453 – 47523Combined sources
Beta strandi478 – 49518Combined sources
Turni496 – 4994Combined sources
Beta strandi500 – 51718Combined sources
Turni520 – 5223Combined sources
Beta strandi523 – 53412Combined sources
Beta strandi543 – 5453Combined sources
Beta strandi549 – 56012Combined sources
Beta strandi563 – 58422Combined sources
Beta strandi586 – 5883Combined sources
Beta strandi590 – 61223Combined sources
Beta strandi615 – 63016Combined sources
Turni632 – 6365Combined sources
Beta strandi644 – 65512Combined sources
Beta strandi656 – 6583Combined sources
Turni659 – 6624Combined sources
Beta strandi663 – 67210Combined sources
Beta strandi675 – 6784Combined sources
Beta strandi683 – 6853Combined sources
Beta strandi688 – 69710Combined sources
Helixi699 – 7024Combined sources
Beta strandi708 – 7158Combined sources
Beta strandi722 – 7276Combined sources
Beta strandi730 – 7334Combined sources
Beta strandi738 – 74710Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BY3X-ray2.74A34-747[»]
1BY5X-ray2.60A34-747[»]
1FCPX-ray2.70A52-747[»]
1FI1X-ray2.90A52-747[»]
1QFFX-ray2.70A34-747[»]
1QFGX-ray2.50A34-747[»]
1QJQX-ray2.95A34-747[»]
1QKCX-ray3.10A34-747[»]
2FCPX-ray2.50A34-747[»]
2GRXX-ray3.30A/B34-747[»]
ProteinModelPortaliP06971.
SMRiP06971. Positions 53-747.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06971.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1492Ferrichrome binding

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 478TonB box
Motifi730 – 74718TonB C-terminal boxAdd
BLAST

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105E0K. Bacteria.
COG1629. LUCA.
HOGENOMiHOG000260252.
InParanoidiP06971.
KOiK02014.
OMAiTVRENSY.
PhylomeDBiP06971.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSKTAQPK HSLRKIAVVV ATAVSGMSVY AQAAVEPKED TITVTAAPAP
60 70 80 90 100
QESAWGPAAT IAARQSATGT KTDTPIQKVP QSISVVTAEE MALHQPKSVK
110 120 130 140 150
EALSYTPGVS VGTRGASNTY DHLIIRGFAA EGQSQNNYLN GLKLQGNFYN
160 170 180 190 200
DAVIDPYMLE RAEIMRGPVS VLYGKSSPGG LLNMVSKRPT TEPLKEVQFK
210 220 230 240 250
AGTDSLFQTG FDFSDSLDDD GVYSYRLTGL ARSANAQQKG SEEQRYAIAP
260 270 280 290 300
AFTWRPDDKT NFTFLSYFQN EPETGYYGWL PKEGTVEPLP NGKRLPTDFN
310 320 330 340 350
EGAKNNTYSR NEKMVGYSFD HEFNDTFTVR QNLRFAENKT SQNSVYGYGV
360 370 380 390 400
CSDPANAYSK QCAALAPADK GHYLARKYVV DDEKLQNFSV DTQLQSKFAT
410 420 430 440 450
GDIDHTLLTG VDFMRMRNDI NAWFGYDDSV PLLNLYNPVN TDFDFNAKDP
460 470 480 490 500
ANSGPYRILN KQKQTGVYVQ DQAQWDKVLV TLGGRYDWAD QESLNRVAGT
510 520 530 540 550
TDKRDDKQFT WRGGVNYLFD NGVTPYFSYS ESFEPSSQVG KDGNIFAPSK
560 570 580 590 600
GKQYEVGVKY VPEDRPIVVT GAVYNLTKTN NLMADPEGSF FSVEGGEIRA
610 620 630 640 650
RGVEIEAKAA LSASVNVVGS YTYTDAEYTT DTTYKGNTPA QVPKHMASLW
660 670 680 690 700
ADYTFFDGPL SGLTLGTGGR YTGSSYGDPA NSFKVGSYTV VDALVRYDLA
710 720 730 740
RVGMAGSNVA LHVNNLFDRE YVASCFNTYG CFWGAERQVV ATATFRF
Length:747
Mass (Da):82,182
Last modified:November 1, 1997 - v2
Checksum:i1C2B251D1990E444
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti737 – 7371R → P in CAA29253 (PubMed:2823072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12486 Genomic DNA. Translation: AAB61768.1.
U00096 Genomic DNA. Translation: AAC73261.1.
AP009048 Genomic DNA. Translation: BAB96726.2.
U70214 Genomic DNA. Translation: AAB08580.1.
X05810 Genomic DNA. Translation: CAA29253.1.
PIRiF64738. QRECFE.
RefSeqiNP_414692.1. NC_000913.3.
WP_000124438.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73261; AAC73261; b0150.
BAB96726; BAB96726; BAB96726.
GeneIDi944856.
KEGGiecj:JW0146.
eco:b0150.
PATRICi32115409. VBIEscCol129921_0156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12486 Genomic DNA. Translation: AAB61768.1.
U00096 Genomic DNA. Translation: AAC73261.1.
AP009048 Genomic DNA. Translation: BAB96726.2.
U70214 Genomic DNA. Translation: AAB08580.1.
X05810 Genomic DNA. Translation: CAA29253.1.
PIRiF64738. QRECFE.
RefSeqiNP_414692.1. NC_000913.3.
WP_000124438.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BY3X-ray2.74A34-747[»]
1BY5X-ray2.60A34-747[»]
1FCPX-ray2.70A52-747[»]
1FI1X-ray2.90A52-747[»]
1QFFX-ray2.70A34-747[»]
1QFGX-ray2.50A34-747[»]
1QJQX-ray2.95A34-747[»]
1QKCX-ray3.10A34-747[»]
2FCPX-ray2.50A34-747[»]
2GRXX-ray3.30A/B34-747[»]
ProteinModelPortaliP06971.
SMRiP06971. Positions 53-747.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259745. 124 interactions.
DIPiDIP-9602N.
IntActiP06971. 5 interactions.
STRINGi511145.b0150.

Protein family/group databases

TCDBi1.B.14.1.2. the outer membrane receptor (omr) family.

Proteomic databases

EPDiP06971.
PaxDbiP06971.
PRIDEiP06971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73261; AAC73261; b0150.
BAB96726; BAB96726; BAB96726.
GeneIDi944856.
KEGGiecj:JW0146.
eco:b0150.
PATRICi32115409. VBIEscCol129921_0156.

Organism-specific databases

EchoBASEiEB0298.
EcoGeneiEG10302. fhuA.

Phylogenomic databases

eggNOGiENOG4105E0K. Bacteria.
COG1629. LUCA.
HOGENOMiHOG000260252.
InParanoidiP06971.
KOiK02014.
OMAiTVRENSY.
PhylomeDBiP06971.

Enzyme and pathway databases

BioCyciEcoCyc:EG10302-MONOMER.
ECOL316407:JW0146-MONOMER.
MetaCyc:EG10302-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP06971.
PROiP06971.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFHUA_ECOLI
AccessioniPrimary (citable) accession number: P06971
Secondary accession number(s): P71280, P75665
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 172 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.