P06960 (OTC2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase chain F
EC=2.1.3.3

Alternative name(s):
OTCase-2

Gene names

Name:	argF
Ordered Locus Names:	b0273, JW0266

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subunit structure	Trimer of identical or non-identical chains, which are coded by two duplicated genes (argI and argF) at different loci.
Subcellular location	Cytoplasm HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 334	333	Ornithine carbamoyltransferase chain F HAMAP MF_01109		PRO_0000112919
Regions
	Region	56 – 60	5	Carbamoyl phosphate binding By similarity
	Region	273 – 276	4	Ornithine binding By similarity
Sites
	Binding site	107	1	Carbamoyl phosphate By similarity
	Binding site	134	1	Carbamoyl phosphate By similarity
	Site	31	1	Important for structural integrity By similarity
	Site	147	1	Important for structural integrity By similarity
Experimental info
	Sequence conflict	121	1	A → P in CAA25329. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P06960 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: C1EA07CD2AFC39C4	FASTA	334	36,827
	10 20 30 40 50 60 MSDLYKKHFL KLLDFTPAQF TSLLTLAAQL KADKKNGKEV QKLTGKNIAL IFEKDSTRTR 70 80 90 100 110 120 CSFEVAAFDQ GARVTYLGPS GSQIGHKESI KDTARVLGRM YDGIQYRGHG QEVVETLAQY 130 140 150 160 170 180 AGVPVWNGLT NEFHPTQLLA DLMTMQEHLP GKAFNEMTLV YAGDARNNMG NSMLEAAALT 190 200 210 220 230 240 GLDLRLLAPK ACWPEESLVA ECSALAEKHG GKITLTEDVA AGVKGADFIY TDVWVSMGEA 250 260 270 280 290 300 KEKWAERIAL LRGYQVNAQM MALTDNPNVK FLHCLPAFHD DQTTLGKQMA KEFDLHGGME 310 320 330 VTDEVFESAA SIVFDQAENR MHTIKAVMMA TLGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferases -- complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB." van Vliet F., Cunin R., Jacobs A., Piette J., Gigot D., Lauwereys M., Pierard A., Glansdorff N. Nucleic Acids Res. 12:6277-6289(1984) [PubMed: 6382166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria." Falmagne P., Portetelle D., Stalon V. J. Bacteriol. 161:714-719(1985) [PubMed: 3968036] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-41.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X00759 Genomic DNA. Translation: CAA25329.1. U70214 Genomic DNA. Translation: AAB08694.1. U00096 Genomic DNA. Translation: AAC73376.1. AP009048 Genomic DNA. Translation: BAE76057.1.
PIR	OWECF. A64753.
RefSeq	NP_414807.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P06960.
SMR	P06960. Positions 2-334.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06960. 7 interactions.
2D gel databases
SWISS-2DPAGE	P06960.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002273; EBESCP00000002273; EBESCG00000001860. EBESCT00000016806; EBESCP00000016097; EBESCG00000015865.
GeneID	944844.
GenomeReviews	Gene locus JW0266 in contig AP009048_GR. Gene locus b0273 in contig U00096_GR.
KEGG	ecj:JW0266. eco:b0273.
PATRIC	32115667. VBIEscCol129921_0277.
Organism-specific databases
EchoBASE	EB0065.
EcoGene	EG10067. argF.
Phylogenomic databases
eggNOG	COG0078.
GeneTree	EBGT00050000009122.
HOGENOM	HBG579429.
OMA	GNIICTE.
PhylomeDB	P06960.
ProtClustDB	PRK12562.
Enzyme and pathway databases
BioCyc	EcoCyc:CHAINF-MONOMER. MetaCyc:CHAINF-MONOMER.
Gene expression databases
Genevestigator	P06960.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC2_ECOLI

Accession

Primary (citable) accession number: P06960
Secondary accession number(s): P78308, Q2MCE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 115 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program