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Protein

Recombinase cre

Gene

cre

Organism
Enterobacteria phage P1 (Bacteriophage P1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei173 – 1731
Active sitei289 – 2891
Active sitei292 – 2921
Active sitei324 – 3241O-(3'-phospho-DNA)-tyrosine intermediate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA integration, DNA recombination

Names & Taxonomyi

Protein namesi
Recommended name:
Recombinase cre
Gene namesi
Name:cre
OrganismiEnterobacteria phage P1 (Bacteriophage P1)
Taxonomic identifieri10678 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaePunalikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]
Proteomesi
  • UP000008091 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Recombinase crePRO_0000197529Add
BLAST

Interactioni

Subunit structurei

Homotetramer when bound to DNA.

Structurei

Secondary structure

1
343
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni16 – 183Combined sources
Helixi21 – 3212Combined sources
Helixi34 – 363Combined sources
Helixi39 – 5921Combined sources
Turni63 – 664Combined sources
Helixi68 – 8013Combined sources
Helixi85 – 10218Combined sources
Helixi107 – 1093Combined sources
Helixi111 – 12616Combined sources
Helixi139 – 14911Combined sources
Helixi155 – 17016Combined sources
Helixi174 – 1796Combined sources
Helixi182 – 1843Combined sources
Beta strandi185 – 1895Combined sources
Beta strandi192 – 1976Combined sources
Beta strandi202 – 2054Combined sources
Beta strandi209 – 2135Combined sources
Helixi215 – 22814Combined sources
Helixi230 – 2323Combined sources
Beta strandi233 – 2353Combined sources
Beta strandi236 – 2394Combined sources
Beta strandi241 – 2433Combined sources
Turni244 – 2463Combined sources
Beta strandi247 – 2493Combined sources
Beta strandi252 – 2543Combined sources
Helixi258 – 27316Combined sources
Beta strandi278 – 2803Combined sources
Beta strandi282 – 2854Combined sources
Helixi290 – 30112Combined sources
Helixi306 – 3138Combined sources
Beta strandi316 – 3205Combined sources
Helixi321 – 3244Combined sources
Turni325 – 3273Combined sources
Helixi329 – 3313Combined sources
Helixi334 – 3396Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CRXX-ray2.40A/B20-341[»]
1DRGX-ray2.55A21-343[»]
1F44X-ray2.05A20-343[»]
1KBUX-ray2.20A/B1-343[»]
1MA7X-ray2.30A/B1-343[»]
1NZBX-ray3.10A/B/E/F1-343[»]
1OUQX-ray3.20A/B/E/F1-343[»]
1PVPX-ray2.35A/B2-343[»]
1PVQX-ray2.75A/B2-343[»]
1PVRX-ray2.65A/B2-343[»]
1Q3UX-ray2.90A/B/E/F1-343[»]
1Q3VX-ray2.91A/B/E/F1-343[»]
1XNSX-ray2.80A/B20-343[»]
1XO0X-ray2.00A/B20-343[»]
2CRXX-ray2.50A/B1-343[»]
2HOFX-ray2.40A/B1-343[»]
2HOIX-ray2.60A/B/G/H1-343[»]
3C28X-ray2.60A/B20-341[»]
3C29X-ray2.20A/B/G/H20-341[»]
3CRXX-ray2.50A/B1-343[»]
3MGVX-ray2.29A/B/C/D1-343[»]
4CRXX-ray2.20A/B20-341[»]
5CRXX-ray2.70A/B1-343[»]
ProteinModelPortaliP06956.
SMRiP06956. Positions 10-341.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06956.

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR010998. Integrase_Lambda-type_N.
IPR023109. Integrase_recombinase_N.
[Graphical view]
PfamiPF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF47823. SSF47823. 1 hit.
SSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

P06956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLLTVHQN LPALPVDATS DEVRKNLMDM FRDRQAFSEH TWKMLLSVCR
60 70 80 90 100
SWAAWCKLNN RKWFPAEPED VRDYLLYLQA RGLAVKTIQQ HLGQLNMLHR
110 120 130 140 150
RSGLPRPSDS NAVSLVMRRI RKENVDAGER AKQALAFERT DFDQVRSLME
160 170 180 190 200
NSDRCQDIRN LAFLGIAYNT LLRIAEIARI RVKDISRTDG GRMLIHIGRT
210 220 230 240 250
KTLVSTAGVE KALSLGVTKL VERWISVSGV ADDPNNYLFC RVRKNGVAAP
260 270 280 290 300
SATSQLSTRA LEGIFEATHR LIYGAKDDSG QRYLAWSGHS ARVGAARDMA
310 320 330 340
RAGVSIPEIM QAGGWTNVNI VMNYIRNLDS ETGAMVRLLE DGD
Length:343
Mass (Da):38,540
Last modified:January 1, 1988 - v1
Checksum:iA05AC4300003FF34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03453 Genomic DNA. Translation: CAA27178.1.
AF234172 Genomic DNA. Translation: AAQ13978.1.
PIRiB24836. RGBPP1.
RefSeqiYP_006472.1. NC_005856.1.

Genome annotation databases

GeneIDi2777477.
KEGGivg:2777477.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03453 Genomic DNA. Translation: CAA27178.1.
AF234172 Genomic DNA. Translation: AAQ13978.1.
PIRiB24836. RGBPP1.
RefSeqiYP_006472.1. NC_005856.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CRXX-ray2.40A/B20-341[»]
1DRGX-ray2.55A21-343[»]
1F44X-ray2.05A20-343[»]
1KBUX-ray2.20A/B1-343[»]
1MA7X-ray2.30A/B1-343[»]
1NZBX-ray3.10A/B/E/F1-343[»]
1OUQX-ray3.20A/B/E/F1-343[»]
1PVPX-ray2.35A/B2-343[»]
1PVQX-ray2.75A/B2-343[»]
1PVRX-ray2.65A/B2-343[»]
1Q3UX-ray2.90A/B/E/F1-343[»]
1Q3VX-ray2.91A/B/E/F1-343[»]
1XNSX-ray2.80A/B20-343[»]
1XO0X-ray2.00A/B20-343[»]
2CRXX-ray2.50A/B1-343[»]
2HOFX-ray2.40A/B1-343[»]
2HOIX-ray2.60A/B/G/H1-343[»]
3C28X-ray2.60A/B20-341[»]
3C29X-ray2.20A/B/G/H20-341[»]
3CRXX-ray2.50A/B1-343[»]
3MGVX-ray2.29A/B/C/D1-343[»]
4CRXX-ray2.20A/B20-341[»]
5CRXX-ray2.70A/B1-343[»]
ProteinModelPortaliP06956.
SMRiP06956. Positions 10-341.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2777477.
KEGGivg:2777477.

Miscellaneous databases

EvolutionaryTraceiP06956.

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR010998. Integrase_Lambda-type_N.
IPR023109. Integrase_recombinase_N.
[Graphical view]
PfamiPF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF47823. SSF47823. 1 hit.
SSF56349. SSF56349. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Bacteriophage P1 cre gene and its regulatory region. Evidence for multiple promoters and for regulation by DNA methylation."
    Sternberg N., Sauer B., Hoess R., Abremski K.
    J. Mol. Biol. 187:197-212(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse."
    Guo F., Gopaul D.N., van Duyne G.D.
    Nature 389:40-46(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
  4. "Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination."
    Gopaul D.N., Guo F., van Duyne G.D.
    EMBO J. 17:4175-4187(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiRECR_BPP1
AccessioniPrimary (citable) accession number: P06956
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 14, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.