P06956 (RECR_BPP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Recombinase cre

Gene names

Name:

cre

Organism

Enterobacteria phage P1 (Bacteriophage P1)

Taxonomic identifier

10678 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › P1-like viruses

Virus host

Enterobacteriaceae [TaxID: 543]

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
Subunit structure	Homotetramer when bound to DNA.
Sequence similarities	Belongs to the 'phage' integrase family.

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 343

343

Recombinase cre

PRO_0000197529

Sites

Active site

173

1

Active site

289

1

Active site

292

1

Active site

324

1

O-(3'-phospho-DNA)-tyrosine intermediate

Secondary structure

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343

Helix Strand Turn

Details...

Helix

21 – 32

12

Helix

34 – 36

3

Helix

39 – 59

21

Helix

68 – 80

13

Helix

85 – 101

17

Helix

107 – 109

3

Helix

111 – 127

17

Helix

139 – 150

12

Helix

155 – 170

16

Helix

174 – 179

6

Helix

182 – 184

3

Beta strand

185 – 187

3

Helix

189 – 191

3

Beta strand

193 – 197

5

Beta strand

202 – 205

4

Beta strand

209 – 212

4

Helix

215 – 226

12

Helix

230 – 232

3

Beta strand

236 – 239

4

Helix

258 – 273

16

Helix

290 – 301

12

Helix

306 – 313

8

Beta strand

316 – 318

3

Helix

321 – 324

4

Helix

329 – 331

3

Helix

334 – 339

6

Sequences

	Sequence		Length	Mass (Da)
	P06956 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: A05AC4300003FF34	FASTA	343	38,540
	10 20 30 40 50 60 MSNLLTVHQN LPALPVDATS DEVRKNLMDM FRDRQAFSEH TWKMLLSVCR SWAAWCKLNN 70 80 90 100 110 120 RKWFPAEPED VRDYLLYLQA RGLAVKTIQQ HLGQLNMLHR RSGLPRPSDS NAVSLVMRRI 130 140 150 160 170 180 RKENVDAGER AKQALAFERT DFDQVRSLME NSDRCQDIRN LAFLGIAYNT LLRIAEIARI 190 200 210 220 230 240 RVKDISRTDG GRMLIHIGRT KTLVSTAGVE KALSLGVTKL VERWISVSGV ADDPNNYLFC 250 260 270 280 290 300 RVRKNGVAAP SATSQLSTRA LEGIFEATHR LIYGAKDDSG QRYLAWSGHS ARVGAARDMA 310 320 330 340 RAGVSIPEIM QAGGWTNVNI VMNYIRNLDS ETGAMVRLLE DGD

References

[1]	"Bacteriophage P1 cre gene and its regulatory region. Evidence for multiple promoters and for regulation by DNA methylation." Sternberg N., Sauer B., Hoess R., Abremski K. J. Mol. Biol. 187:197-212(1986) [PubMed: 3486297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome of bacteriophage P1." Lobocka M.B., Rose D.J., Plunkett G. III, Rusin M., Samojedny A., Lehnherr H., Yarmolinsky M.B., Blattner F.R. J. Bacteriol. 186:7032-7068(2004) [PubMed: 15489417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse." Guo F., Gopaul D.N., van Duyne G.D. Nature 389:40-46(1997) [PubMed: 9288963] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
[4]	"Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination." Gopaul D.N., Guo F., van Duyne G.D. EMBO J. 17:4175-4187(1998) [PubMed: 9670032] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03453 Genomic DNA. Translation: CAA27178.1.
AF234172 Genomic DNA. Translation: AAQ13978.1.

PIR

RGBPP1. B24836.

RefSeq

YP_006472.1. NC_005856.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CRX	X-ray	2.40	A/B	20-341	[»]
1DRG	X-ray	2.55	A	21-343	[»]
1F44	X-ray	2.05	A	20-343	[»]
1KBU	X-ray	2.20	A/B	2-343	[»]
1MA7	X-ray	2.30	A/B	2-343	[»]
1NZB	X-ray	3.10	A/B/E/F	1-343	[»]
1OUQ	X-ray	3.20	A/B/E/F	1-343	[»]
1PVP	X-ray	2.35	A/B	2-343	[»]
1PVQ	X-ray	2.75	A/B	2-343	[»]
1PVR	X-ray	2.65	A/B	2-343	[»]
1Q3U	X-ray	2.90	A/B/E/F	1-343	[»]
1Q3V	X-ray	2.91	A/B/E/F	1-343	[»]
1XNS	X-ray	2.80	A/B	20-343	[»]
1XO0	X-ray	2.00	A/B	20-343	[»]
2CRX	X-ray	2.50	A/B	1-343	[»]
2HOF	X-ray	2.40	A/B	1-343	[»]
2HOI	X-ray	2.60	A/B/G/H	1-343	[»]
3C28	X-ray	2.60	A/B	20-341	[»]
3C29	X-ray	2.20	A/B/G/H	20-341	[»]
3CRX	X-ray	2.50	A/B	1-343	[»]
3MGV	X-ray	2.29	A/B/C/D	1-343	[»]
4CRX	X-ray	2.20	A/B	20-341	[»]
5CRX	X-ray	2.70	A/B	1-343	[»]

ProteinModelPortal

P06956.

SMR

P06956. Positions 10-341.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2777477.

GenomeReviews

Gene locus cre in contig AF234172_GR.

Family and domain databases

InterPro

IPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat-core.
IPR002104. Integrase_catalytic.
IPR010998. Integrase_Lambda-type_N.
IPR023109. Integrase_recombinase_N.
[Graphical view]

Gene3D

G3DSA:1.10.150.130. Integrase_recombinase_N. 1 hit.
G3DSA:1.10.443.10. Phage_intgr_like. 1 hit.

Pfam

PF00589. Phage_integrase. 1 hit.
[Graphical view]

SUPFAM

SSF56349. DNA_brk_join_enz. 1 hit.
SSF47823. L_intgrse_like_N. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RECR_BPP1

Accession

Primary (citable) accession number: P06956

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	September 21, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program