P06939 (VPX_HV2RO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Vpx

Alternative name(s):
Viral protein X
X ORF protein

Gene names

Name:

vpx

Organism

Human immunodeficiency virus type 2 subtype A (isolate ROD) (HIV-2) [Complete proteome]

Taxonomic identifier

11720 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Primate lentivirus group

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. May be necessary for virus replication. Ref.2
Subunit structure	Interacts with the P6 region of unprocessed GAG. Ref.3
Subcellular location	Virion. Host nucleus. Note: Nuclear just after virion uncoating, or if expressed in the absence of unprocessed GAG.

Ontologies

Keywords
Cellular component	Host nucleus Virion
Disease	AIDS
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 112	112	Protein Vpx		PRO_0000085397
Regions
	Motif	65 – 72	8	Nuclear localization signal
Experimental info
	Mutagenesis	63 – 65	3	SES → AEA: No effect on non-dividing cells infection. Ref.4
	Mutagenesis	66 – 71	6	YTKYRY → ATKARA: Partial loss of non-dividing cells infection. Ref.4
	Mutagenesis	66 – 71	6	YTKYRY → ATKYRA: No effect on non-dividing cells infection. Ref.4
	Mutagenesis	66 – 71	6	YTKYRY → FTKFRF: No effect on non-dividing cells infection. Ref.4
	Mutagenesis	66 – 69	4	YTKY → ATKA: Partial loss of non-dividing cells infection.
	Mutagenesis	66	1	Y → A: No effect on non-dividing cells infection.
	Mutagenesis	68 – 70	3	KYR → AYA: Complete loss of non-dividing cells infection.
	Mutagenesis	69 – 71	3	YRY → ARA: Partial loss of non-dividing cells infection.
	Mutagenesis	69	1	Y → A: Partial loss of non-dividing cells infection.
	Mutagenesis	71	1	Y → A: No effect on non-dividing cells infection.

Sequences

	Sequence		Length	Mass (Da)
	P06939 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 0A677EB6BDB5F665	FASTA	112	12,815
	10 20 30 40 50 60 MTDPRETVPP GNSGEETIGE AFAWLNRTVE AINREAVNHL PRELIFQVWQ RSWRYWHDEQ 70 80 90 100 110 GMSESYTKYR YLCIIQKAVY MHVRKGCTCL GRGHGPGGWR PGPPPPPPPG LV

References

[1]	"Genome organization and transactivation of the human immunodeficiency virus type 2." Guyader M., Emerman M., Sonigo P., Clavel F., Montagnier L., Alizon M. Nature 326:662-669(1987) [PubMed: 3031510] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of HIV-2 Vpx by modeling and insertional mutagenesis." Mahnke L.A., Belshan M., Ratner L. Virology 348:165-174(2006) [PubMed: 16457868] [Abstract] Cited for: FUNCTION.
[3]	"HIV type 2 Vpx interaction with Gag and incorporation into virus-like particles." Jin L., Zhou Y., Ratner L. AIDS Res. Hum. Retroviruses 17:105-111(2001) [PubMed: 11177390] [Abstract] Cited for: INTERACTION WITH GAG.
[4]	"Conserved amino acids of the human immunodeficiency virus type 2 Vpx nuclear localization signal are critical for nuclear targeting of the viral preintegration complex in non-dividing cells." Belshan M., Mahnke L.A., Ratner L. Virology 346:118-126(2006) [PubMed: 16325220] [Abstract] Cited for: MUTAGENESIS OF 63-SER--SER-65 AND 66-TYR--TYR-71.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15390 Genomic DNA. Translation: AAB00766.1. X05291 Genomic RNA. No translation available.
PIR	ASLJX2. I26262.
3D structure databases
ProteinModelPortal	P06939.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000012. RetroV_VpR/X. [Graphical view]
Pfam	PF00522. VPR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VPX_HV2RO

Accession

Primary (citable) accession number: P06939

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	May 3, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program