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Protein

Regulatory protein E2

Gene

E2

Organism
Human papillomavirus type 5
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processDNA replication, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein E2UniRule annotation
Gene namesi
Name:E2UniRule annotation
OrganismiHuman papillomavirus type 5
Taxonomic identifieri333923 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeBetapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009252 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001331821 – 514Regulatory protein E2Add BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki437Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation

Post-translational modificationi

Phosphorylated.UniRule annotation
Sumoylation plays a regulatory role in E2 transcriptional activity.UniRule annotation

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Binds DNA as homodimer. Interacts with protein E1; this interaction greatly increases E1 DNA-binding activity. Interacts with protein L1; this interaction enhances E2-dependent replication and transcription activation. Interacts with protein L2; this interaction inhibits E2 transcriptional activity but not DNA replication function E2. Interacts with protein E7; this interaction inhibits E7 oncogenic activity. Interacts with host TAF1; this interaction modulates E2-dependent transcriptional regulation. Interacts with host BRD4; this interaction mediates E2 transcriptional activation function. Additionally, the interaction with host BRD4 on mitotic chromosomes mediates tethering of the viral genome. Interacts with host TOPBP1; this interaction is required for optimal viral DNA replication.UniRule annotation

Protein-protein interaction databases

IntActiP06921, 87 interactors
MINTiP06921

Structurei

3D structure databases

ProteinModelPortaliP06921
SMRiP06921
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 201Transactivation domainUniRule annotationAdd BLAST201
Regioni430 – 514DNA-binding domainUniRule annotationAdd BLAST85

Sequence similaritiesi

Belongs to the papillomaviridae E2 protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900010D

Family and domain databases

Gene3Di3.30.70.330, 1 hit
HAMAPiMF_04001 PPV_E2, 1 hit
InterProiView protein in InterPro
IPR035975 E2/EBNA1_C_sf
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR000427 Papillomavirus_E2_C
IPR001866 Papillomavirus_E2_N
IPR033668 Reg_prot_E2
IPR036050 Regulatory_protein_E2_N
PfamiView protein in Pfam
PF00511 PPV_E2_C, 1 hit
PF00508 PPV_E2_N, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000672 E2_early_recog_prot_C, 1 hit
SUPFAMiSSF51332 SSF51332, 1 hit
SSF54957 SSF54957, 1 hit

Sequencei

Sequence statusi: Complete.

P06921-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENLSERFNA LQDQLMNIYE AAEQTLQAQI KHWQTLRKEP VLLYYAREKG
60 70 80 90 100
VTRLGYQPVP VKAVSETKAK EAIAMVLQLE SLQTSDFAHE PWTLVDTSIE
110 120 130 140 150
TFRSAPEGHF KKGPLPVEVI YDNDPDNANL YTMWTYVYYM DADDKWHKAR
160 170 180 190 200
SGVNHIGIYY LQGTFKNYYV LFADDAKRYG TTGEWEVKVN KETVFAPVTS
210 220 230 240 250
STPPGSPGGQ ADTNTTPATP TTSTTAVDST SRQLTTSKQP QQTETRGRRY
260 270 280 290 300
GRRPSSKSRR SQTQQRRSRS RHRSRSRSRS RSKSQTHTTR STTRSRSTSL
310 320 330 340 350
TKTRALTSRS RSRGRSPTTC RRGGGRSPRR RSRSPSTSSS CTTQRSQRAR
360 370 380 390 400
AESSTTRGAR GSRGSRGGSR GGRGRRRGRS SSSSSPAHKR SRGGSAKLRG
410 420 430 440 450
VSPGEVGGSL RSVSSKHTGR LGRLLEEARD PPVIIVKGAA NTLKNVRNRA
460 470 480 490 500
KIKYMGLFRS FSTTWSWVAG DGTERLGRPR MLISFSSYTQ RRDFDEAVRY
510
PKGVDKAYGN LDSL
Length:514
Mass (Da):57,532
Last modified:February 1, 1996 - v2
Checksum:i0100722143BF7256
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17463 Genomic DNA Translation: AAA46986.1
PIRiD26277 W2WL5
RefSeqiNP_041368.1, NC_001531.1

Genome annotation databases

GeneIDi1489050
KEGGivg:1489050

Similar proteinsi

Entry informationi

Entry nameiVE2_HPV05
AccessioniPrimary (citable) accession number: P06921
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: February 1, 1996
Last modified: February 28, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health