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P06911

- LCN5_RAT

UniProt

P06911 - LCN5_RAT

Protein

Epididymal-specific lipocalin-5

Gene

Lcn5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Associates with spermatozoa in the epididymal fluid but does not bind tightly to them. Binds both all-trans and 9-cis retinoic acid. May act as a retinoid carrier protein which is required for epididymal function and/or sperm maturation.

    GO - Molecular functioni

    1. transporter activity Source: InterPro

    GO - Biological processi

    1. lipid metabolic process Source: InterPro

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epididymal-specific lipocalin-5
    Alternative name(s):
    Androgen-dependent epididymal 18.5 kDa protein
    Epididymal retinoic acid-binding protein
    Short name:
    E-RABP
    Epididymal secretory protein I
    Short name:
    ESP-I
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Lcn5
    Synonyms:Erabp
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi69320. Lcn5.

    Subcellular locationi

    Secreted
    Note: Synthesized in the proximal part of the epididymis and secreted into the epididymal fluid.

    GO - Cellular componenti

    1. extracellular region Source: RGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19191 PublicationAdd
    BLAST
    Chaini20 – 188169Epididymal-specific lipocalin-5, C formPRO_0000339294Add
    BLAST
    Chaini23 – 188166Epididymal-specific lipocalin-5, B formPRO_0000017869Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi82 ↔ 176

    Post-translational modificationi

    There are two similar, immunologically cross-reacting forms of this protein, designated B and C, which probably result from different processing of the amino end.
    The N-terminus of form C is probably blocked.

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP06911.
    PRIDEiP06911.

    Expressioni

    Tissue specificityi

    Synthesized exclusively in the proximal part (caput epididymidis) of the epididymis. It makes up a substantial part of the total protein in the epididymal luminal fluid and binds to the sperm membrane.

    Gene expression databases

    GenevestigatoriP06911.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000058570.

    Structurei

    Secondary structure

    1
    188
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 334
    Beta strandi35 – 4410
    Beta strandi59 – 668
    Beta strandi69 – 7810
    Beta strandi81 – 9111
    Beta strandi97 – 1026
    Beta strandi105 – 11410
    Beta strandi116 – 12813
    Beta strandi131 – 14111
    Helixi147 – 15913
    Helixi164 – 1663
    Beta strandi167 – 1693
    Helixi175 – 1828

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EPAX-ray2.10A/B23-186[»]
    1EPBX-ray2.20A/B23-186[»]
    ProteinModelPortaliP06911.
    SMRiP06911. Positions 23-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP06911.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the calycin superfamily. Lipocalin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGieuNOG08191.
    HOGENOMiHOG000113294.
    HOVERGENiHBG096015.
    InParanoidiP06911.
    PhylomeDBiP06911.
    TreeFamiTF336103.

    Family and domain databases

    Gene3Di2.40.128.20. 1 hit.
    InterProiIPR012674. Calycin.
    IPR011038. Calycin-like.
    IPR022272. Lipocalin_CS.
    IPR000566. Lipocln_cytosolic_FA-bd_dom.
    IPR002972. PstgldnD_synth.
    [Graphical view]
    PfamiPF00061. Lipocalin. 1 hit.
    [Graphical view]
    PRINTSiPR01254. PGNDSYNTHASE.
    SUPFAMiSSF50814. SSF50814. 1 hit.
    PROSITEiPS00213. LIPOCALIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06911-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENIMPFALL GLCVGLAAGT EGAVVKDFDI SKFLGFWYEI AFASKMGTPG    50
    LAHKEEKMGA MVVELKENLL ALTTTYYSED HCVLEKVTAT EGDGPAKFQV 100
    TRLSGKKEVV VEATDYLTYA IIDITSLVAG AVHRTMKLYS RSLDDNGEAL 150
    YNFRKITSDH GFSETDLYIL KHDLTCVKVL QSAAESRP 188
    Length:188
    Mass (Da):20,670
    Last modified:August 1, 1992 - v2
    Checksum:i8248BBB4C2D95355
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59831 Genomic DNA. Translation: CAA42493.1.
    X59832 mRNA. Translation: CAA42494.1.
    M12790 mRNA. Translation: AAA41127.1.
    PIRiA43801. SQRTAD.
    RefSeqiNP_077050.1. NM_024136.1.
    UniGeneiRn.10362.

    Genome annotation databases

    GeneIDi29552.
    KEGGirno:29552.
    UCSCiRGD:69320. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59831 Genomic DNA. Translation: CAA42493.1 .
    X59832 mRNA. Translation: CAA42494.1 .
    M12790 mRNA. Translation: AAA41127.1 .
    PIRi A43801. SQRTAD.
    RefSeqi NP_077050.1. NM_024136.1.
    UniGenei Rn.10362.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EPA X-ray 2.10 A/B 23-186 [» ]
    1EPB X-ray 2.20 A/B 23-186 [» ]
    ProteinModelPortali P06911.
    SMRi P06911. Positions 23-186.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000058570.

    Proteomic databases

    PaxDbi P06911.
    PRIDEi P06911.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 29552.
    KEGGi rno:29552.
    UCSCi RGD:69320. rat.

    Organism-specific databases

    CTDi 13863.
    RGDi 69320. Lcn5.

    Phylogenomic databases

    eggNOGi euNOG08191.
    HOGENOMi HOG000113294.
    HOVERGENi HBG096015.
    InParanoidi P06911.
    PhylomeDBi P06911.
    TreeFami TF336103.

    Miscellaneous databases

    EvolutionaryTracei P06911.
    NextBioi 609578.

    Gene expression databases

    Genevestigatori P06911.

    Family and domain databases

    Gene3Di 2.40.128.20. 1 hit.
    InterProi IPR012674. Calycin.
    IPR011038. Calycin-like.
    IPR022272. Lipocalin_CS.
    IPR000566. Lipocln_cytosolic_FA-bd_dom.
    IPR002972. PstgldnD_synth.
    [Graphical view ]
    Pfami PF00061. Lipocalin. 1 hit.
    [Graphical view ]
    PRINTSi PR01254. PGNDSYNTHASE.
    SUPFAMi SSF50814. SSF50814. 1 hit.
    PROSITEi PS00213. LIPOCALIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An 18-kDa androgen-regulated protein that modifies galactosyltransferase activity is synthesized by the rat caput epididymidis, but has no structural similarity to rat milk alphalactalbumin."
      Moore A., Hall L., Hamilton D.W.
      Biol. Reprod. 43:497-506(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Epididymis.
    2. "Structure and expression of the rat epididymal secretory protein I gene. An androgen-regulated member of the lipocalin superfamily with a rare splice donor site."
      Girotti M., Jones R., Emery D.C., Chia W., Hall L.
      Biochem. J. 281:203-210(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
    3. "Molecular cloning of the cDNA for two major androgen-dependent secretory proteins of 18.5 kilodaltons synthesized by the rat epididymis."
      Brooks D.E., Means A.R., Wright E.J., Singh S.P., Tiver K.K.
      J. Biol. Chem. 261:4956-4961(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-188, PROTEIN SEQUENCE OF 23-40.
    4. "Purification and crystallization of a retinoic acid-binding protein from rat epididymis. Identity with the major androgen-dependent epididymal proteins."
      Newcomer M.E., Ong D.E.
      J. Biol. Chem. 265:12876-12879(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-47, CHARACTERIZATION.
    5. Lubec G., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 58-66, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    6. "Structure of the epididymal retinoic acid binding protein at 2.1-A resolution."
      Newcomer M.E.
      Structure 1:7-18(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

    Entry informationi

    Entry nameiLCN5_RAT
    AccessioniPrimary (citable) accession number: P06911
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3