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Protein

Transcriptional activator Myb

Gene

Myb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi63 – 86H-T-H motifPROSITE-ProRule annotationAdd BLAST24
DNA bindingi115 – 138H-T-H motifPROSITE-ProRule annotationAdd BLAST24
DNA bindingi166 – 189H-T-H motifPROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • DNA binding Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • WD40-repeat domain binding Source: MGI

GO - Biological processi

  • B cell differentiation Source: MGI
  • calcium ion transport Source: MGI
  • cellular response to interleukin-6 Source: MGI
  • chromatin remodeling Source: MGI
  • embryonic digestive tract development Source: MGI
  • G1/S transition of mitotic cell cycle Source: MGI
  • homeostasis of number of cells Source: MGI
  • in utero embryonic development Source: MGI
  • myeloid cell differentiation Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of histone H3-K4 methylation Source: MGI
  • positive regulation of histone H3-K9 methylation Source: MGI
  • positive regulation of T-helper cell differentiation Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of gene expression Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • spleen development Source: MGI
  • stem cell division Source: MGI
  • thymus development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator Myb
Alternative name(s):
Proto-oncogene c-Myb
Gene namesi
Name:Myb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97249. Myb.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001970491 – 636Transcriptional activator MybAdd BLAST636

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei467N6-acetyllysineBy similarity1
Modified residuei476N6-acetyllysineBy similarity1
Modified residuei528PhosphoserineBy similarity1
Modified residuei530PhosphothreonineBy similarity1

Post-translational modificationi

Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.By similarity
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP06876.
PaxDbiP06876.
PRIDEiP06876.

PTM databases

iPTMnetiP06876.
PhosphoSitePlusiP06876.

Expressioni

Gene expression databases

BgeeiENSMUSG00000019982.
CleanExiMM_MYB.
ExpressionAtlasiP06876. baseline and differential.
GenevisibleiP06876. MM.

Interactioni

Subunit structurei

Binds to HIPK1 (By similarity). Interacts with HIPK2, MAF, MYBBP1A and NLK.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Hipk2Q9QZR52EBI-366934,EBI-366905

GO - Molecular functioni

  • WD40-repeat domain binding Source: MGI

Protein-protein interaction databases

BioGridi201631. 12 interactors.
DIPiDIP-31713N.
IntActiP06876. 2 interactors.
STRINGi10090.ENSMUSP00000020158.

Structurei

Secondary structure

1636
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi45 – 58Combined sources14
Helixi63 – 68Combined sources6
Helixi75 – 86Combined sources12
Beta strandi92 – 94Combined sources3
Helixi97 – 110Combined sources14
Helixi115 – 119Combined sources5
Helixi127 – 136Combined sources10
Beta strandi140 – 142Combined sources3
Beta strandi144 – 146Combined sources3
Helixi149 – 162Combined sources14
Helixi163 – 165Combined sources3
Helixi166 – 170Combined sources5
Beta strandi173 – 175Combined sources3
Helixi178 – 188Combined sources11
Helixi189 – 191Combined sources3
Helixi292 – 311Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GUUX-ray1.60A38-89[»]
1GV2X-ray1.68A89-193[»]
1GV5X-ray1.58A90-141[»]
1GVDX-ray1.45A90-141[»]
1H88X-ray2.80C37-193[»]
1H89X-ray2.45C37-193[»]
1IDYNMR-A141-193[»]
1IDZNMR-A141-193[»]
1MBENMR-A38-89[»]
1MBFNMR-A38-89[»]
1MBGNMR-A90-141[»]
1MBHNMR-A90-141[»]
1MBJNMR-A142-193[»]
1MBKNMR-A142-193[»]
1MSENMR-C90-193[»]
1MSFNMR-C90-193[»]
1SB0NMR-B291-315[»]
2AGHNMR-A291-315[»]
ProteinModelPortaliP06876.
SMRiP06876.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06876.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 86HTH myb-type 1PROSITE-ProRule annotationAdd BLAST52
Domaini87 – 142HTH myb-type 2PROSITE-ProRule annotationAdd BLAST56
Domaini143 – 193HTH myb-type 3PROSITE-ProRule annotationAdd BLAST51

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 193Interaction with HIPK2 and NLK1 PublicationAdd BLAST104
Regioni275 – 327Transcriptional activation domainBy similarityAdd BLAST53
Regioni328 – 460Negative regulatory domainAdd BLAST133
Regioni375 – 396Leucine-zipperAdd BLAST22

Domaini

Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
C-terminal truncated mutants display increased transactivation.

Sequence similaritiesi

Contains 3 HTH myb-type DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0048. Eukaryota.
COG5147. LUCA.
GeneTreeiENSGT00390000001038.
HOVERGENiHBG007964.
InParanoidiP06876.
KOiK09420.
TreeFamiTF326257.

Family and domain databases

Gene3Di1.10.10.60. 3 hits.
InterProiIPR015395. C-myb_C.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR012642. Tscrpt_reg_Wos2-domain.
[Graphical view]
PfamiPF09316. Cmyb_C. 1 hit.
PF07988. LMSTEN. 1 hit.
PF00249. Myb_DNA-binding. 3 hits.
[Graphical view]
SMARTiSM00717. SANT. 3 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51294. HTH_MYB. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRPRHSIY SSDEDDEDIE MCDHDYDGLL PKSGKRHLGK TRWTREEDEK
60 70 80 90 100
LKKLVEQNGT DDWKVIANYL PNRTDVQCQH RWQKVLNPEL IKGPWTKEED
110 120 130 140 150
QRVIELVQKY GPKRWSVIAK HLKGRIGKQC RERWHNHLNP EVKKTSWTEE
160 170 180 190 200
EDRIIYQAHK RLGNRWAEIA KLLPGRTDNA IKNHWNSTMR RKVEQEGYLQ
210 220 230 240 250
EPSKASQTPV ATSFQKNNHL MGFGHASPPS QLSPSGQSSV NSEYPYYHIA
260 270 280 290 300
EAQNISSHVP YPVALHVNIV NVPQPAAAAI QRHYNDEDPE KEKRIKELEL
310 320 330 340 350
LLMSTENELK GQQALPTQNH TCSYPGWHST SIVDQTRPHG DSAPVSCLGE
360 370 380 390 400
HHATPSLPAD PGSLPEESAS PARCMIVHQG TILDNVKNLL EFAETLQFID
410 420 430 440 450
SFLNTSSNHE SSGLDAPTLP STPLIGHKLT PCRDQTVKTQ KENSIFRTPA
460 470 480 490 500
IKRSILESSP RTPTPFKHAL AAQEIKYGPL KMLPQTPSHA VEDLQDVIKQ
510 520 530 540 550
ESDESGIVAE FQESGPPLLK KIKQEVESPT EKSGNFFCSN HWAENSLSTQ
560 570 580 590 600
LFSQASPVAD APNILTSSVL MTPVSEDEDN VLKAFTVPKN RPLVGPLQPC
610 620 630
SGAWEPASCG KTEDQMTASG PARKYVNAFS ARTLVM
Length:636
Mass (Da):71,422
Last modified:July 27, 2011 - v2
Checksum:i0C6308E584726D01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105E → K in CAA26552 (PubMed:2998780).Curated1
Sequence conflicti201E → K in CAA26552 (PubMed:2998780).Curated1
Sequence conflicti267V → A in AAB59713 (PubMed:3010282).Curated1
Sequence conflicti402F → V in AAA39786 (PubMed:3014527).Curated1
Sequence conflicti411S → N in CAA26552 (PubMed:2998780).Curated1
Sequence conflicti500Q → R in CAA26552 (PubMed:2998780).Curated1
Sequence conflicti500Q → R in AAH11513 (PubMed:15489334).Curated1
Sequence conflicti525E → A in CAA26552 (PubMed:2998780).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21169 Genomic DNA. Translation: AAA39782.1.
M12848 mRNA. Translation: AAB59713.1.
X02774 mRNA. Translation: CAA26552.1.
M20210 Genomic DNA. Translation: AAA39783.1.
AC153556 Genomic DNA. No translation available.
BC011513 mRNA. Translation: AAH11513.1.
X04099
, X04100, X04101, X04102, X04103, X04104 Genomic DNA. Translation: CAA27724.1.
X16389 Genomic DNA. Translation: CAA34425.1.
X16390 Genomic DNA. Translation: CAA34426.1.
M13989 Genomic DNA. Translation: AAA39787.1.
K03547 Genomic DNA. Translation: AAA39786.1.
CCDSiCCDS35861.1.
PIRiA25285. TVMSMB.
RefSeqiNP_001185843.1. NM_001198914.1.
NP_034978.3. NM_010848.3.
UniGeneiMm.473872.
Mm.52109.

Genome annotation databases

EnsembliENSMUST00000020158; ENSMUSP00000020158; ENSMUSG00000019982.
GeneIDi17863.
KEGGimmu:17863.
UCSCiuc007eog.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21169 Genomic DNA. Translation: AAA39782.1.
M12848 mRNA. Translation: AAB59713.1.
X02774 mRNA. Translation: CAA26552.1.
M20210 Genomic DNA. Translation: AAA39783.1.
AC153556 Genomic DNA. No translation available.
BC011513 mRNA. Translation: AAH11513.1.
X04099
, X04100, X04101, X04102, X04103, X04104 Genomic DNA. Translation: CAA27724.1.
X16389 Genomic DNA. Translation: CAA34425.1.
X16390 Genomic DNA. Translation: CAA34426.1.
M13989 Genomic DNA. Translation: AAA39787.1.
K03547 Genomic DNA. Translation: AAA39786.1.
CCDSiCCDS35861.1.
PIRiA25285. TVMSMB.
RefSeqiNP_001185843.1. NM_001198914.1.
NP_034978.3. NM_010848.3.
UniGeneiMm.473872.
Mm.52109.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GUUX-ray1.60A38-89[»]
1GV2X-ray1.68A89-193[»]
1GV5X-ray1.58A90-141[»]
1GVDX-ray1.45A90-141[»]
1H88X-ray2.80C37-193[»]
1H89X-ray2.45C37-193[»]
1IDYNMR-A141-193[»]
1IDZNMR-A141-193[»]
1MBENMR-A38-89[»]
1MBFNMR-A38-89[»]
1MBGNMR-A90-141[»]
1MBHNMR-A90-141[»]
1MBJNMR-A142-193[»]
1MBKNMR-A142-193[»]
1MSENMR-C90-193[»]
1MSFNMR-C90-193[»]
1SB0NMR-B291-315[»]
2AGHNMR-A291-315[»]
ProteinModelPortaliP06876.
SMRiP06876.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201631. 12 interactors.
DIPiDIP-31713N.
IntActiP06876. 2 interactors.
STRINGi10090.ENSMUSP00000020158.

PTM databases

iPTMnetiP06876.
PhosphoSitePlusiP06876.

Proteomic databases

EPDiP06876.
PaxDbiP06876.
PRIDEiP06876.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020158; ENSMUSP00000020158; ENSMUSG00000019982.
GeneIDi17863.
KEGGimmu:17863.
UCSCiuc007eog.1. mouse.

Organism-specific databases

CTDi4602.
MGIiMGI:97249. Myb.

Phylogenomic databases

eggNOGiKOG0048. Eukaryota.
COG5147. LUCA.
GeneTreeiENSGT00390000001038.
HOVERGENiHBG007964.
InParanoidiP06876.
KOiK09420.
TreeFamiTF326257.

Miscellaneous databases

EvolutionaryTraceiP06876.
PROiP06876.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019982.
CleanExiMM_MYB.
ExpressionAtlasiP06876. baseline and differential.
GenevisibleiP06876. MM.

Family and domain databases

Gene3Di1.10.10.60. 3 hits.
InterProiIPR015395. C-myb_C.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR012642. Tscrpt_reg_Wos2-domain.
[Graphical view]
PfamiPF09316. Cmyb_C. 1 hit.
PF07988. LMSTEN. 1 hit.
PF00249. Myb_DNA-binding. 3 hits.
[Graphical view]
SMARTiSM00717. SANT. 3 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51294. HTH_MYB. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYB_MOUSE
AccessioniPrimary (citable) accession number: P06876
Secondary accession number(s): E9QMG8, Q61929
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 171 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.