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Reviewed, UniProtKB/Swiss-Prot P06874 (THER_BACST)

Last modified June 16, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Thermolysin
    EC=3.4.24.27
Alternative name(s):
    Thermostable neutral proteinase
Gene names
Name: nprT
OrganismBacillus stearothermophilus (Geobacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

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Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Extracellular zinc metalloprotease.

Catalytic activity

Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.

Cofactor

Binds 4 calcium ions per subunit.

Binds 1 zinc ion per subunit.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M4 family.

biophysicochemical properties

Temperature dependence:

Thermostable.

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Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMZymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525
Propeptide26 – 229204Activation peptide Ref.1
PRO_0000028590
Chain230 – 548319Thermolysin
PRO_0000028591

Sites

Active site3751
Active site4631Proton donor
Metal binding2891Calcium 1 By similarity
Metal binding2911Calcium 1 By similarity
Metal binding2931Calcium 1; via carbonyl oxygen By similarity
Metal binding3701Calcium 2 By similarity
Metal binding3741Zinc; catalytic
Metal binding3781Zinc; catalytic
Metal binding3981Zinc; catalytic
Metal binding4091Calcium 2 By similarity
Metal binding4091Calcium 3 By similarity
Metal binding4151Calcium 3; via carbonyl oxygen By similarity
Metal binding4171Calcium 2 By similarity
Metal binding4171Calcium 3 By similarity
Metal binding4191Calcium 2; via carbonyl oxygen By similarity
Metal binding4221Calcium 2 By similarity
Metal binding4221Calcium 3 By similarity
Metal binding4251Calcium 4; via carbonyl oxygen By similarity
Metal binding4261Calcium 4 By similarity
Metal binding4291Calcium 4; via carbonyl oxygen By similarity
Metal binding4321Calcium 4 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P06874-1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 5B4D2C1D7910539D

FASTA54859,580
        10         20         30         40         50         60 
MNKRAMLGAI GLAFGLLAAP IGASAKGESI VWNEQWKTPS FVSGSLLNGG EQALEELVYQ 

        70         80         90        100        110        120 
YVDRENGTFR LGGRARDRLA LIGKQTDELG HTVMRFEQRH HGIPVYGTML AAHVKDGELI 

       130        140        150        160        170        180 
ALSGSLIPNL DGQPRLKKAK TVTVQQAEAI AEQDVTETVT KERPTTENGE RTRLVIYPTD 

       190        200        210        220        230        240 
GTARLAYEVN VRFLTPVPGN WVYIIDATDG AILNKFNQID SRQPGGGQPV AGASTVGVGR 

       250        260        270        280        290        300 
GVLGDQKYIN TTYSSYYGYY YLQDNTRGSG IFTYDGRNRT VLPGSLWTDG DNQFTASYDA 

       310        320        330        340        350        360 
AAVDAHYYAG VVYDYYKNVH GRLSYDGSNA AIRSTVHYGR GYNNAFWNGS QMVYGDGDGQ 

       370        380        390        400        410        420 
TFLPFSGGID VVGHELTHAV TDYTAGLVYQ NESGAINEAM SDIFGTLVEF YANRNPDWEI 

       430        440        450        460        470        480 
GEDIYTPGVA GDALRSMSDP AKYGDPDHYS KRYTGTQDNG GVHTNSGIIN KAAYLLSQGG 

       490        500        510        520        530        540 
VHYGVSVNGI GRDKMGKIFY RALVYYLTPT SNFSQLRAAC VQAAADLYGS TSQEVNSVKQ 


AFNAVGVY

« Hide

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References

[1]"Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus."
Takagi M., Imanaka T., Aiba S.
J. Bacteriol. 163:824-831(1985) [PubMed: 2993245] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 230-243.

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Cross-references

Sequence databases

M11446 Genomic DNA. Translation: AAA22621.1.
PIRHYBSS. A24924.

3D structure databases

HSSPHSSP built from PDB template 1KJO based on UniProtKB P00800.
ModBaseSearch...

Protein family/group databases

MEROPSM04.001.

Enzyme and pathway databases

BRENDA3.4.24.27. 266715.

Family and domain databases

InterProIPR005075. Pept_M4_propep_PepSY.
IPR006025. Pept_M_Zn_BS.
IPR013856. Peptidase_M4.
IPR001570. Peptidase_M4_C.
IPR011096. Propep_M4_M36.
[Graphical view]
Gene3DG3DSA:3.10.170.10. Peptidase_M4. 1 hit.
G3DSA:1.10.390.10. Peptidase_M4/M36. 1 hit.
PfamPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSPR00730. THERMOLYSIN.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameTHER_BACST
AccessionPrimary (citable) accession number: P06874
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents