P06871 (TRY1_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cationic trypsin EC=3.4.21.4
Organism	Canis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifier	9615 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 23	8	Activation peptide		PRO_0000028191
	Chain	24 – 246	223	Cationic trypsin		PRO_0000028192
Regions
	Domain	24 – 244	221	Peptidase S1
Sites
	Active site	63	1	Charge relay system By similarity
	Active site	107	1	Charge relay system By similarity
	Active site	200	1	Charge relay system By similarity
	Metal binding	75	1	Calcium By similarity
	Metal binding	77	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	85	1	Calcium By similarity
	Site	194	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	30 ↔ 160		By similarity
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	132 ↔ 233		By similarity
	Disulfide bond	139 ↔ 206		By similarity
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	196 ↔ 220		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06871 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: E9E5A1DE2391BBBB	FASTA	246	26,170
	10 20 30 40 50 60 MKTFIFLALL GATVAFPIDD DDKIVGGYTC SRNSVPYQVS LNSGYHFCGG SLINSQWVVS 70 80 90 100 110 120 AAHCYKSRIQ VRLGEYNIAV SEGGEQFINA AKIIRHPRYN ANTIDNDIML IKLSSPATLN 130 140 150 160 170 180 SRVSAIALPK SCPAAGTQCL ISGWGNTQSI GQNYPDVLQC LKAPILSDSV CRNAYPGQIS 190 200 210 220 230 240 SNMMCLGYME GGKDSCQGDS GGPVVCNGEL QGVVSWGAGC AQKGKPGVSP KVCKYVSWIQ QTIAAN

References

[1]	"Differential regulation of trypsinogen mRNA translation: full-length mRNA sequences encoding two oppositely charged trypsinogen isoenzymes in the dog pancreas." Pinsky S.D., Laforge K.S., Scheele G. Mol. Cell. Biol. 5:2669-2676(1985) [PubMed: 3841794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11590 mRNA. Translation: AAA30900.1.
PIR	TRDGC. B26273.
3D structure databases
ProteinModelPortal	P06871.
SMR	P06871. Positions 24-246.
ModBase	Search...
Protein-protein interaction databases
STRING	P06871.
Protein family/group databases
MEROPS	S01.151.
Proteomic databases
PRIDE	P06871.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	maNOG08762.
GeneTree	ENSGT00580000081320.
HOVERGEN	HBG013304.
InParanoid	P06871.
OrthoDB	EOG4SJ5FV.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY1_CANFA

Accession

Primary (citable) accession number: P06871

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program