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P06869

- UROK_MOUSE

UniProt

P06869 - UROK_MOUSE

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Protein

Urokinase-type plasminogen activator

Gene

Plau

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei226 – 2261Charge relay system
Active sitei277 – 2771Charge relay system
Active sitei378 – 3781Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. fibrinolysis Source: MGI
  2. regulation of cell adhesion mediated by integrin Source: Ensembl
  3. regulation of cell proliferation Source: MGI
  4. regulation of receptor activity Source: Ensembl
  5. regulation of smooth muscle cell-matrix adhesion Source: Ensembl
  6. regulation of smooth muscle cell migration Source: Ensembl
  7. response to hypoxia Source: MGI
  8. smooth muscle cell migration Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Plasminogen activation

Protein family/group databases

MEROPSiS01.231.

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:Plau
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:97611. Plau.

Subcellular locationi

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. extracellular space Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 433413Urokinase-type plasminogen activatorPRO_0000028322Add
BLAST
Chaini21 – 178158Urokinase-type plasminogen activator long chain ABy similarityPRO_0000028323Add
BLAST
Chaini157 – 17822Urokinase-type plasminogen activator short chain ABy similarityPRO_0000028324Add
BLAST
Chaini180 – 433254Urokinase-type plasminogen activator chain BBy similarityPRO_0000028325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 401 Publication
Disulfide bondi34 ↔ 521 Publication
Disulfide bondi54 ↔ 631 Publication
Disulfide bondi71 ↔ 1521 Publication
Disulfide bondi92 ↔ 1341 Publication
Disulfide bondi123 ↔ 1471 Publication
Modified residuei159 – 1591PhosphoserineBy similarity
Disulfide bondi169 ↔ 301Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondi211 ↔ 227By similarity
Disulfide bondi219 ↔ 290By similarity
Disulfide bondi315 ↔ 384By similarity
Disulfide bondi347 ↔ 363By similarity
Disulfide bondi374 ↔ 402By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

MaxQBiP06869.
PaxDbiP06869.
PRIDEiP06869.

PTM databases

PhosphoSiteiP06869.

Expressioni

Gene expression databases

BgeeiP06869.
CleanExiMM_PLAU.
ExpressionAtlasiP06869. baseline and differential.
GenevestigatoriP06869.

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).By similarity

Protein-protein interaction databases

BioGridi202230. 1 interaction.
IntActiP06869. 3 interactions.
MINTiMINT-4139656.
STRINGi10090.ENSMUSP00000022368.

Structurei

Secondary structure

1
433
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi40 – 423
Helixi44 – 463
Beta strandi50 – 523
Turni60 – 634
Beta strandi65 – 684
Helixi100 – 1023
Beta strandi103 – 1064
Turni110 – 1167
Beta strandi118 – 1203
Beta strandi133 – 1386
Beta strandi141 – 1477

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LAQX-ray3.20A/B21-154[»]
ProteinModelPortaliP06869.
SMRiP06869. Positions 28-152, 180-424.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06869.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 6437EGF-likePROSITE-ProRule annotationAdd
BLAST
Domaini71 – 15282KringlePROSITE-ProRule annotationAdd
BLAST
Domaini180 – 426247Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 5824Binds urokinase plasminogen activator surface receptorBy similarityAdd
BLAST
Regioni153 – 17927Connecting peptideAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 kringle domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000237314.
HOVERGENiHBG008633.
InParanoidiP06869.
KOiK01348.
OMAiKMLCAAD.
OrthoDBiEOG75B84T.
PhylomeDBiP06869.
TreeFamiTF329901.

Family and domain databases

Gene3Di2.40.20.10. 1 hit.
InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00181. EGF. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06869 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVWLASLFL CALVVKNSEG GSVLGAPDES NCGCQNGGVC VSYKYFSRIR
60 70 80 90 100
RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV
110 120 130 140 150
LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH
160 170 180 190 200
DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK
210 220 230 240 250
NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN
260 270 280 290 300
PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI
310 320 330 340 350
CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP
360 370 380 390 400
HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR
410 420 430
GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF
Length:433
Mass (Da):48,268
Last modified:January 1, 1988 - v1
Checksum:iA99C35F6250443F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02389 mRNA. Translation: CAA26231.1.
M17922 Genomic DNA. Translation: AAA40539.1.
CCDSiCCDS26858.1.
PIRiA29420. UKMS.
RefSeqiNP_032899.1. NM_008873.3.
UniGeneiMm.4183.

Genome annotation databases

EnsembliENSMUST00000022368; ENSMUSP00000022368; ENSMUSG00000021822.
GeneIDi18792.
KEGGimmu:18792.
UCSCiuc007skx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02389 mRNA. Translation: CAA26231.1 .
M17922 Genomic DNA. Translation: AAA40539.1 .
CCDSi CCDS26858.1.
PIRi A29420. UKMS.
RefSeqi NP_032899.1. NM_008873.3.
UniGenei Mm.4183.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3LAQ X-ray 3.20 A/B 21-154 [» ]
ProteinModelPortali P06869.
SMRi P06869. Positions 28-152, 180-424.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202230. 1 interaction.
IntActi P06869. 3 interactions.
MINTi MINT-4139656.
STRINGi 10090.ENSMUSP00000022368.

Chemistry

ChEMBLi CHEMBL1075311.

Protein family/group databases

MEROPSi S01.231.

PTM databases

PhosphoSitei P06869.

Proteomic databases

MaxQBi P06869.
PaxDbi P06869.
PRIDEi P06869.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022368 ; ENSMUSP00000022368 ; ENSMUSG00000021822 .
GeneIDi 18792.
KEGGi mmu:18792.
UCSCi uc007skx.2. mouse.

Organism-specific databases

CTDi 5328.
MGIi MGI:97611. Plau.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000237314.
HOVERGENi HBG008633.
InParanoidi P06869.
KOi K01348.
OMAi KMLCAAD.
OrthoDBi EOG75B84T.
PhylomeDBi P06869.
TreeFami TF329901.

Miscellaneous databases

EvolutionaryTracei P06869.
NextBioi 295072.
PROi P06869.
SOURCEi Search...

Gene expression databases

Bgeei P06869.
CleanExi MM_PLAU.
ExpressionAtlasi P06869. baseline and differential.
Genevestigatori P06869.

Family and domain databases

Gene3Di 2.40.20.10. 1 hit.
InterProi IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00181. EGF. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEi PS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase-type plasminogen activator."
    Belin D., Vassalli J.-D., Combepine C., Godeau F., Nagamine Y., Reich E., Kocher H.P., Duvoisin R.M.
    Eur. J. Biochem. 148:225-232(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The murine urokinase-type plasminogen activator gene."
    Degen S.J.F., Heckel J.L., Reich E., Degen J.L.
    Biochemistry 26:8270-8279(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: implication for preclinical cancer therapy."
    Lin L., Gardsvoll H., Huai Q., Huang M., Ploug M.
    J. Biol. Chem. 285:10982-10992(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 21-154 IN COMPLEX WITH PLAUR, DISULFIDE BONDS.

Entry informationi

Entry nameiUROK_MOUSE
AccessioniPrimary (citable) accession number: P06869
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 29, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3