Reviewed, UniProtKB/Swiss-Prot P06869 (UROK_MOUSE)

Names and origin

Protein names

Recommended name:
    Urokinase-type plasminogen activator
      Short name=U-plasminogen activator
      Short name=uPA
    EC=3.4.21.73
Cleaved into the following 3 chains:
    1- Recommended name:
            Urokinase-type plasminogen activator long chain A
    2- Recommended name:
            Urokinase-type plasminogen activator short chain A
    3- Recommended name:
            Urokinase-type plasminogen activator chain B

Gene names

Name:

Plau

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
Catalytic activity	Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Subunit structure	Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 EGF-like domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Plasminogen activation
Cellular component	Secreted
Domain	EGF-like domain Kringle Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro regulation of cell proliferation Inferred from genetic interaction. Source: MGI response to hypoxia Inferred from mutant phenotype. Source: MGI smooth muscle cell migration Inferred from mutant phenotype. Source: MGI
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 433	413	Urokinase-type plasminogen activator		PRO_0000028322
	Chain	21 – 178	158	Urokinase-type plasminogen activator long chain A By similarity		PRO_0000028323
	Chain	157 – 178	22	Urokinase-type plasminogen activator short chain A By similarity		PRO_0000028324
	Chain	180 – 433	254	Urokinase-type plasminogen activator chain B By similarity		PRO_0000028325
Regions
	Domain	28 – 64	37	EGF-like
	Domain	71 – 152	82	Kringle
	Domain	180 – 426	247	Peptidase S1
	Region	35 – 58	24	Binds urokinase plasminogen activator surface receptor By similarity
	Region	153 – 179	27	Connecting peptide
Sites
	Active site	226	1	Charge relay system
	Active site	277	1	Charge relay system
	Active site	378	1	Charge relay system
Amino acid modifications
	Disulfide bond	32 ↔ 40		By similarity
	Disulfide bond	34 ↔ 52		By similarity
	Disulfide bond	54 ↔ 63		By similarity
	Disulfide bond	71 ↔ 152		By similarity
	Disulfide bond	92 ↔ 134		By similarity
	Disulfide bond	123 ↔ 147		By similarity
	Disulfide bond	169 ↔ 301		Interchain (between A and B chains) By similarity
	Disulfide bond	211 ↔ 227		By similarity
	Disulfide bond	219 ↔ 290		By similarity
	Disulfide bond	315 ↔ 384		By similarity
	Disulfide bond	347 ↔ 363		By similarity
	Disulfide bond	374 ↔ 402		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06869-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: A99C35F6250443F9	FASTA	433	48,268
	10 20 30 40 50 60 MKVWLASLFL CALVVKNSEG GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG 70 80 90 100 110 120 EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH 130 140 150 160 170 180 NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI 190 200 210 220 230 240 VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY 250 260 270 280 290 300 LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI 310 320 330 340 350 360 CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK 370 380 390 400 410 420 MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW 430 IQSHIGEEKG LAF

References

[1]	"Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase-type plasminogen activator." Belin D., Vassalli J.-D., Combepine C., Godeau F., Nagamine Y., Reich E., Kocher H.P., Duvoisin R.M. Eur. J. Biochem. 148:225-232(1985) [PubMed: 2985383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The murine urokinase-type plasminogen activator gene." Degen S.J.F., Heckel J.L., Reich E., Degen J.L. Biochemistry 26:8270-8279(1987) [PubMed: 2831940] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X02389 mRNA. Translation: CAA26231.1. M17922 Genomic DNA. Translation: AAA40539.1.
IPI	IPI00129102.
PIR	UKMS. A29420.
RefSeq	NP_032899.1.
UniGene	Mm.4183
3D structure databases
HSSP	HSSP built from PDB template 1KDU based on UniProtKB P00749.
SMR	P06869. Positions 30-153, 180-428.
ModBase	Search...
Protein family/group databases
MEROPS	S01.231.
PTM databases
PhosphoSite	P06869.
Genome annotation databases
Ensembl	ENSMUSG00000021822. Mus musculus. [Contig view]
GeneID	18792.
KEGG	mmu:18792.
Organism-specific databases
MGI	MGI:97611. Plau.
Phylogenomic databases
HOGENOM	P06869.
HOVERGEN	P06869.
OMA	P06869. QPWFAAI.
Enzyme and pathway databases
BRENDA	3.4.21.73. 244.
Gene expression databases
ArrayExpress	P06869.
Bgee	P06869.
CleanEx	MM_PLAU.
GermOnline	ENSMUSG00000021822. Mus musculus.
Family and domain databases
InterPro	IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR013111. EGF_extracell. IPR000001. Kringle. IPR018056. Kringle_CS. IPR018059. Kringle_sub. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Gene3D	G3DSA:2.40.20.10. Kringle. 1 hit.
Pfam	PF07974. EGF_2. 1 hit. PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN. PR00018. KRINGLE.
ProDom	PD000395. Kringle. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00181. EGF. 1 hit. SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. False negative. PS50026. EGF_3. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	295072.
SOURCE	Search...

Entry information

Entry name

UROK_MOUSE

Accession

Primary (citable) accession number: P06869

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)