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Protein

Urokinase-type plasminogen activator

Gene

Plau

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226Charge relay system1
Active sitei277Charge relay system1
Active sitei378Charge relay system1

GO - Molecular functioni

GO - Biological processi

  • fibrinolysis Source: MGI
  • positive regulation of cell migration Source: MGI
  • proteolysis Source: GO_Central
  • regulation of cell adhesion mediated by integrin Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of receptor activity Source: MGI
  • regulation of smooth muscle cell-matrix adhesion Source: MGI
  • regulation of smooth muscle cell migration Source: MGI
  • response to hypoxia Source: MGI
  • smooth muscle cell migration Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Plasminogen activation

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-75205. Dissolution of Fibrin Clot.

Protein family/group databases

MEROPSiS01.231.

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:Plau
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:97611. Plau.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075311.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002832221 – 433Urokinase-type plasminogen activatorAdd BLAST413
ChainiPRO_000002832321 – 178Urokinase-type plasminogen activator long chain ABy similarityAdd BLAST158
ChainiPRO_0000028324157 – 178Urokinase-type plasminogen activator short chain ABy similarityAdd BLAST22
ChainiPRO_0000028325180 – 433Urokinase-type plasminogen activator chain BBy similarityAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 401 Publication
Disulfide bondi34 ↔ 521 Publication
Disulfide bondi54 ↔ 631 Publication
Disulfide bondi71 ↔ 1521 Publication
Disulfide bondi92 ↔ 1341 Publication
Disulfide bondi123 ↔ 1471 Publication
Modified residuei159PhosphoserineBy similarity1
Disulfide bondi169 ↔ 301Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondi211 ↔ 227By similarity
Disulfide bondi219 ↔ 290By similarity
Disulfide bondi315 ↔ 384By similarity
Disulfide bondi347 ↔ 363By similarity
Disulfide bondi374 ↔ 402By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP06869.
PeptideAtlasiP06869.
PRIDEiP06869.

PTM databases

PhosphoSitePlusiP06869.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021822.
CleanExiMM_PLAU.
ExpressionAtlasiP06869. baseline and differential.
GenevisibleiP06869. MM.

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).By similarity

Protein-protein interaction databases

BioGridi202230. 1 interactor.
IntActiP06869. 3 interactors.
MINTiMINT-4139656.
STRINGi10090.ENSMUSP00000022368.

Chemistry databases

BindingDBiP06869.

Structurei

Secondary structure

1433
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 42Combined sources3
Helixi44 – 46Combined sources3
Beta strandi50 – 52Combined sources3
Turni60 – 63Combined sources4
Beta strandi65 – 68Combined sources4
Helixi100 – 102Combined sources3
Beta strandi103 – 106Combined sources4
Turni110 – 116Combined sources7
Beta strandi118 – 120Combined sources3
Beta strandi133 – 138Combined sources6
Beta strandi141 – 147Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LAQX-ray3.20A/B21-154[»]
ProteinModelPortaliP06869.
SMRiP06869.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06869.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 64EGF-likePROSITE-ProRule annotationAdd BLAST37
Domaini71 – 152KringlePROSITE-ProRule annotationAdd BLAST82
Domaini180 – 426Peptidase S1PROSITE-ProRule annotationAdd BLAST247

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 58Binds urokinase plasminogen activator surface receptorBy similarityAdd BLAST24
Regioni153 – 179Connecting peptideAdd BLAST27

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 kringle domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiENOG410IGFI. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119133.
HOGENOMiHOG000237314.
HOVERGENiHBG008633.
InParanoidiP06869.
KOiK01348.
OMAiKMLCAAD.
OrthoDBiEOG091G0AH5.
PhylomeDBiP06869.
TreeFamiTF329901.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVWLASLFL CALVVKNSEG GSVLGAPDES NCGCQNGGVC VSYKYFSRIR
60 70 80 90 100
RCSCPRKFQG EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV
110 120 130 140 150
LQKPYNAHRP DAISLGLGKH NYCRNPDNQK RPWCYVQIGL RQFVQECMVH
160 170 180 190 200
DCSLSKKPSS SVDQQGFQCG QKALRPRFKI VGGEFTEVEN QPWFAAIYQK
210 220 230 240 250
NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY LGQSKESSYN
260 270 280 290 300
PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI
310 320 330 340 350
CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP
360 370 380 390 400
HYYGSEINYK MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR
410 420 430
GCAEKNKPGV YTRVSHFLDW IQSHIGEEKG LAF
Length:433
Mass (Da):48,268
Last modified:January 1, 1988 - v1
Checksum:iA99C35F6250443F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02389 mRNA. Translation: CAA26231.1.
M17922 Genomic DNA. Translation: AAA40539.1.
CCDSiCCDS26858.1.
PIRiA29420. UKMS.
RefSeqiNP_032899.1. NM_008873.3.
XP_017171408.1. XM_017315919.1.
UniGeneiMm.4183.

Genome annotation databases

EnsembliENSMUST00000022368; ENSMUSP00000022368; ENSMUSG00000021822.
GeneIDi18792.
KEGGimmu:18792.
UCSCiuc007skx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02389 mRNA. Translation: CAA26231.1.
M17922 Genomic DNA. Translation: AAA40539.1.
CCDSiCCDS26858.1.
PIRiA29420. UKMS.
RefSeqiNP_032899.1. NM_008873.3.
XP_017171408.1. XM_017315919.1.
UniGeneiMm.4183.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LAQX-ray3.20A/B21-154[»]
ProteinModelPortaliP06869.
SMRiP06869.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202230. 1 interactor.
IntActiP06869. 3 interactors.
MINTiMINT-4139656.
STRINGi10090.ENSMUSP00000022368.

Chemistry databases

BindingDBiP06869.
ChEMBLiCHEMBL1075311.

Protein family/group databases

MEROPSiS01.231.

PTM databases

PhosphoSitePlusiP06869.

Proteomic databases

PaxDbiP06869.
PeptideAtlasiP06869.
PRIDEiP06869.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022368; ENSMUSP00000022368; ENSMUSG00000021822.
GeneIDi18792.
KEGGimmu:18792.
UCSCiuc007skx.2. mouse.

Organism-specific databases

CTDi5328.
MGIiMGI:97611. Plau.

Phylogenomic databases

eggNOGiENOG410IGFI. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119133.
HOGENOMiHOG000237314.
HOVERGENiHBG008633.
InParanoidiP06869.
KOiK01348.
OMAiKMLCAAD.
OrthoDBiEOG091G0AH5.
PhylomeDBiP06869.
TreeFamiTF329901.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-75205. Dissolution of Fibrin Clot.

Miscellaneous databases

EvolutionaryTraceiP06869.
PROiP06869.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021822.
CleanExiMM_PLAU.
ExpressionAtlasiP06869. baseline and differential.
GenevisibleiP06869. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUROK_MOUSE
AccessioniPrimary (citable) accession number: P06869
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 167 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.