Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P06869 (UROK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Urokinase-type plasminogen activator

Short name=U-plasminogen activator
Short name=uPA
EC=3.4.21.73
Gene names
Name:Plau
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length433 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activity

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulation

Inhibited by SERPINA5 By similarity.

Subunit structure

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 EGF-like domain.

Contains 1 kringle domain.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 433413Urokinase-type plasminogen activator
PRO_0000028322
Chain21 – 178158Urokinase-type plasminogen activator long chain A By similarity
PRO_0000028323
Chain157 – 17822Urokinase-type plasminogen activator short chain A By similarity
PRO_0000028324
Chain180 – 433254Urokinase-type plasminogen activator chain B By similarity
PRO_0000028325

Regions

Domain28 – 6437EGF-like
Domain71 – 15282Kringle
Domain180 – 426247Peptidase S1
Region35 – 5824Binds urokinase plasminogen activator surface receptor By similarity
Region153 – 17927Connecting peptide

Sites

Active site2261Charge relay system
Active site2771Charge relay system
Active site3781Charge relay system

Amino acid modifications

Modified residue1591Phosphoserine By similarity
Disulfide bond32 ↔ 40 Ref.3
Disulfide bond34 ↔ 52 Ref.3
Disulfide bond54 ↔ 63 Ref.3
Disulfide bond71 ↔ 152 Ref.3
Disulfide bond92 ↔ 134 Ref.3
Disulfide bond123 ↔ 147 Ref.3
Disulfide bond169 ↔ 301Interchain (between A and B chains) By similarity
Disulfide bond211 ↔ 227 By similarity
Disulfide bond219 ↔ 290 By similarity
Disulfide bond315 ↔ 384 By similarity
Disulfide bond347 ↔ 363 By similarity
Disulfide bond374 ↔ 402 By similarity

Secondary structure

...................... 433
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06869 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: A99C35F6250443F9

FASTA43348,268
        10         20         30         40         50         60 
MKVWLASLFL CALVVKNSEG GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG 

        70         80         90        100        110        120 
EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH 

       130        140        150        160        170        180 
NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI 

       190        200        210        220        230        240 
VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY 

       250        260        270        280        290        300 
LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI 

       310        320        330        340        350        360 
CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK 

       370        380        390        400        410        420 
MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW 

       430 
IQSHIGEEKG LAF 

« Hide

References

[1]"Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase-type plasminogen activator."
Belin D., Vassalli J.-D., Combepine C., Godeau F., Nagamine Y., Reich E., Kocher H.P., Duvoisin R.M.
Eur. J. Biochem. 148:225-232(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The murine urokinase-type plasminogen activator gene."
Degen S.J.F., Heckel J.L., Reich E., Degen J.L.
Biochemistry 26:8270-8279(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: implication for preclinical cancer therapy."
Lin L., Gardsvoll H., Huai Q., Huang M., Ploug M.
J. Biol. Chem. 285:10982-10992(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 21-154 IN COMPLEX WITH PLAUR, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02389 mRNA. Translation: CAA26231.1.
M17922 Genomic DNA. Translation: AAA40539.1.
PIRUKMS. A29420.
RefSeqNP_032899.1. NM_008873.3.
UniGeneMm.4183.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3LAQX-ray3.20A/B21-154[»]
ProteinModelPortalP06869.
SMRP06869. Positions 28-152, 180-424.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202230. 1 interaction.
IntActP06869. 3 interactions.
MINTMINT-4139656.
STRING10090.ENSMUSP00000022368.

Chemistry

ChEMBLCHEMBL1075311.

Protein family/group databases

MEROPSS01.231.

PTM databases

PhosphoSiteP06869.

Proteomic databases

PaxDbP06869.
PRIDEP06869.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022368; ENSMUSP00000022368; ENSMUSG00000021822.
GeneID18792.
KEGGmmu:18792.
UCSCuc007skx.2. mouse.

Organism-specific databases

CTD5328.
MGIMGI:97611. Plau.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000237314.
HOVERGENHBG008633.
InParanoidP06869.
KOK01348.
OMAKMLCAAD.
OrthoDBEOG75B84T.
PhylomeDBP06869.
TreeFamTF329901.

Gene expression databases

ArrayExpressP06869.
BgeeP06869.
CleanExMM_PLAU.
GenevestigatorP06869.

Family and domain databases

Gene3D2.40.20.10. 1 hit.
InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00181. EGF. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP06869.
NextBio295072.
PROP06869.
SOURCESearch...

Entry information

Entry nameUROK_MOUSE
AccessionPrimary (citable) accession number: P06869
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: April 16, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot