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Protein

Evolved beta-galactosidase subunit alpha

Gene

ebgA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The wild-type enzyme is an ineffective lactase. Two classes of point mutations dramatically improve activity of the enzyme.

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei449Proton donor1 Publication1
Active sitei512NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG10252-MONOMER
SABIO-RKiP06864

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Evolved beta-galactosidase subunit alpha (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:ebgA
Ordered Locus Names:b3076, JW5511
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10252 ebgA

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

DrugBankiDB00581 Lactulose

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000576511 – 1030Evolved beta-galactosidase subunit alphaAdd BLAST1030

Proteomic databases

PaxDbiP06864
PRIDEiP06864

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta subunits.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
intAP320533EBI-558098,EBI-552967

Protein-protein interaction databases

BioGridi4263419, 6 interactors
DIPiDIP-2893N
IntActiP06864, 7 interactors
STRINGi316385.ECDH10B_3251

Structurei

3D structure databases

ProteinModelPortaliP06864
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
HOGENOMiHOG000252444
InParanoidiP06864
KOiK12111
OMAiWCDHGIL
PhylomeDBiP06864

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
2.70.98.10, 1 hit
InterProiView protein in InterPro
IPR004199 B-gal_small/dom_5
IPR036156 Beta-gal/glucu_dom_sf
IPR011013 Gal_mutarotase_sf_dom
IPR008979 Galactose-bd-like_sf
IPR014718 GH-type_carb-bd
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR032312 LacZ_4
PfamiView protein in Pfam
PF02929 Bgal_small_N, 1 hit
PF16353 DUF4981, 1 hit
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SMARTiView protein in SMART
SM01038 Bgal_small_N, 1 hit
SUPFAMiSSF49303 SSF49303, 2 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit

Sequencei

Sequence statusi: Complete.

P06864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRWENIQLT HENRLAPRAY FFSYDSVAQA RTFARETSSL FLPLSGQWNF
60 70 80 90 100
HFFDHPLQVP EAFTSELMAD WGHITVPAMW QMEGHGKLQY TDEGFPFPID
110 120 130 140 150
VPFVPSDNPT GAYQRIFTLS DGWQGKQTLI KFDGVETYFE VYVNGQYVGF
160 170 180 190 200
SKGSRLTAEF DISAMVKTGD NLLCVRVMQW ADSTYVEDQD MWWSAGIFRD
210 220 230 240 250
VYLVGKHLTH INDFTVRTDF DEAYCDATLS CEVVLENLAA SPVVTTLEYT
260 270 280 290 300
LFDGERVVHS SAIDHLAIEK LTSASFAFTV EQPQQWSAES PYLYHLVMTL
310 320 330 340 350
KDANGNVLEV VPQRVGFRDI KVRDGLFWIN NRYVMLHGVN RHDNDHRKGR
360 370 380 390 400
AVGMDRVEKD LQLMKQHNIN SVRTAHYPND PRFYELCDIY GLFVMAETDV
410 420 430 440 450
ESHGFANVGD ISRITDDPQW EKVYVERIVR HIHAQKNHPS IIIWSLGNES
460 470 480 490 500
GYGCNIRAMY HAAKALDDTR LVHYEEDRDA EVVDIISTMY TRVPLMNEFG
510 520 530 540 550
EYPHPKPRII CEYAHAMGNG PGGLTEYQNV FYKHDCIQGH YVWEWCDHGI
560 570 580 590 600
QAQDDHGNVW YKFGGDYGDY PNNYNFCLDG LIYSDQTPGP GLKEYKQVIA
610 620 630 640 650
PVKIHARDLT RGELKVENKL WFTTLDDYTL HAEVRAEGET LATQQIKLRD
660 670 680 690 700
VAPNSEAPLQ ITLPQLDARE AFLNITVTKD SRTRYSEAGH PIATYQFPLK
710 720 730 740 750
ENTAQPVPFA PNNARPLTLE DDRLSCTVRG YNFAITFSKM SGKPTSWQVN
760 770 780 790 800
GESLLTREPK INFFKPMIDN HKQEYEGLWQ PNHLQIMQEH LRDFAVEQSD
810 820 830 840 850
GEVLIISRTV IAPPVFDFGM RCTYIWRIAA DGQVNVALSG ERYGDYPHII
860 870 880 890 900
PCIGFTMGIN GEYDQVAYYG RGPGENYADS QQANIIDIWR STVDAMFENY
910 920 930 940 950
PFPQNNGNRQ HVRWTALTNR HGNGLLVVPQ RPINFSAWHY TQENIHAAQH
960 970 980 990 1000
CNELQRSDDI TLNLDHQLLG LGSNSWGSEV LDSWRVWFRD FSYGFTLLPV
1010 1020 1030
SGGEATAQSL ASYEFGAGFF STNLHSENKQ
Length:1,030
Mass (Da):117,879
Last modified:October 11, 2004 - v4
Checksum:iFEC4D7A558C6EE94
GO

Sequence cautioni

The sequence AAA57877 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti275S → T (PubMed:2515108).Curated1
Sequence conflicti275S → T (PubMed:3939707).Curated1
Sequence conflicti275S → T in AAA57877 (PubMed:9278503).Curated1
Sequence conflicti465A → R (PubMed:2515108).Curated1
Sequence conflicti465A → R (PubMed:3939707).Curated1
Sequence conflicti640T → S (PubMed:2515108).Curated1
Sequence conflicti640T → S (PubMed:3939707).Curated1
Sequence conflicti649R → P (PubMed:2515108).Curated1
Sequence conflicti649R → P (PubMed:3939707).Curated1
Sequence conflicti767M → MM (PubMed:2515108).Curated1
Sequence conflicti767M → MM (PubMed:3939707).Curated1
Sequence conflicti891 – 892ST → QA (PubMed:2515108).Curated2
Sequence conflicti891 – 892ST → QA (PubMed:3939707).Curated2
Sequence conflicti891 – 892ST → QA in AAA57877 (PubMed:9278503).Curated2
Sequence conflicti1026S → T (PubMed:2515108).Curated1
Sequence conflicti1026S → T (PubMed:3939707).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti92D → N Improve activity. 1
Natural varianti93E → K Improve activity. 1
Natural varianti976W → C Improve activity. 1
Natural varianti978S → G Improve activity. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64441 Genomic DNA Translation: AAA61971.1
X52031 Genomic DNA Translation: CAA36274.1
X03228 Genomic DNA Translation: CAA26977.1 Sequence problems.
U18997 Genomic DNA Translation: AAA57877.1 Different initiation.
U00096 Genomic DNA Translation: AAT48164.1
AP009048 Genomic DNA Translation: BAE77126.1
PIRiA65096 GBECE
RefSeqiWP_001082856.1, NZ_LN832404.1
YP_026199.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48164; AAT48164; b3076
BAE77126; BAE77126; BAE77126
GeneIDi947583
KEGGiecj:JW5511
eco:b3076
PATRICifig|511145.12.peg.3170

Similar proteinsi

Entry informationi

Entry nameiBGA2_ECOLI
AccessioniPrimary (citable) accession number: P06864
Secondary accession number(s): P76660, Q2M9D0, Q6BF50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 161 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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