Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inactive pancreatic lipase-related protein 1

Gene

PNLIPRP1

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as inhibitor of dietary triglyceride digestion. Lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters (in vitro).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei171Nucleophile1
Active sitei194Charge relay system1
Metal bindingi205Calcium; via carbonyl oxygen1
Metal bindingi208Calcium; via carbonyl oxygen1
Metal bindingi210Calcium1
Metal bindingi213Calcium1
Active sitei281Charge relay system1

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • carboxylic ester hydrolase activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.26. 1153.

Protein family/group databases

ESTHERicanfa-1plip. Pancreatic_lipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive pancreatic lipase-related protein 1
Short name:
PL-RP1
Gene namesi
Name:PNLIPRP1
Synonyms:PLRP1
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 28

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi196V → A: Removes steric hindrance and restores lipase activity; when associated with P-198. 1 Publication1
Mutagenesisi198A → P: Removes steric hindrance and restores lipase activity; when associated with A-196. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000001778918 – 467Inactive pancreatic lipase-related protein 1Add BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi21 ↔ 27PROSITE-ProRule annotation1 Publication
Disulfide bondi109 ↔ 120PROSITE-ProRule annotation1 Publication
Glycosylationi157N-linked (GlcNAc...)1 Publication1
Disulfide bondi255 ↔ 279PROSITE-ProRule annotation1 Publication
Disulfide bondi303 ↔ 314PROSITE-ProRule annotation1 Publication
Disulfide bondi317 ↔ 322PROSITE-ProRule annotation1 Publication
Disulfide bondi451 ↔ 467PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP06857.

Expressioni

Tissue specificityi

Detected in pancreas (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000017453.

Structurei

Secondary structure

1467
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 22Combined sources4
Turni23 – 25Combined sources3
Beta strandi26 – 29Combined sources4
Turni32 – 34Combined sources3
Beta strandi35 – 40Combined sources6
Helixi49 – 52Combined sources4
Beta strandi55 – 59Combined sources5
Beta strandi61 – 66Combined sources6
Beta strandi68 – 70Combined sources3
Helixi76 – 79Combined sources4
Beta strandi87 – 93Combined sources7
Helixi104 – 112Combined sources9
Turni113 – 115Combined sources3
Beta strandi118 – 124Combined sources7
Helixi126 – 129Combined sources4
Helixi133 – 158Combined sources26
Helixi162 – 164Combined sources3
Beta strandi165 – 170Combined sources6
Helixi173 – 182Combined sources10
Beta strandi189 – 194Combined sources6
Turni198 – 202Combined sources5
Turni205 – 207Combined sources3
Helixi211 – 213Combined sources3
Beta strandi214 – 220Combined sources7
Helixi227 – 230Combined sources4
Beta strandi240 – 246Combined sources7
Turni247 – 250Combined sources4
Helixi266 – 270Combined sources5
Helixi279 – 293Combined sources15
Turni295 – 298Combined sources4
Helixi306 – 310Combined sources5
Beta strandi324 – 326Combined sources3
Helixi327 – 331Combined sources5
Beta strandi342 – 345Combined sources4
Beta strandi349 – 351Combined sources3
Beta strandi356 – 367Combined sources12
Beta strandi369 – 379Combined sources11
Beta strandi387 – 394Combined sources8
Beta strandi399 – 408Combined sources10
Beta strandi411 – 422Combined sources12
Beta strandi432 – 441Combined sources10
Beta strandi448 – 451Combined sources4
Beta strandi462 – 466Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RP1X-ray2.10A18-467[»]
ProteinModelPortaliP06857.
SMRiP06857.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06857.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini356 – 467PLATPROSITE-ProRule annotationAdd BLAST112

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHRX. Eukaryota.
ENOG410Y92X. LUCA.
GeneTreeiENSGT00760000119069.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiP06857.
KOiK14074.
OMAiKAKEVCY.
OrthoDBiEOG091G0DJ5.
TreeFamiTF324997.

Family and domain databases

CDDicd00707. Pancreat_lipase_like. 1 hit.
Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR013818. Lipase/vitellogenin.
IPR016272. Lipase_LIPH.
IPR033906. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSIWTIALF LLGAAKAKEV CYEQIGCFSD AEPWAGTAIR PLKVLPWSPE
60 70 80 90 100
RIGTRFLLYT NKNPNNFQTL LPSDPSTIEA SNFQTDKKTR FIIHGFIDKG
110 120 130 140 150
EENWLLDMCK NMFKVEEVNC ICVDWKKGSQ TSYTQAANNV RVVGAQVAQM
160 170 180 190 200
LSMLSANYSY SPSQVQLIGH SLGAHVAGEA GSRTPGLGRI TGLDPVEASF
210 220 230 240 250
QGTPEEVRLD PTDADFVDVI HTDAAPLIPF LGFGTSQQMG HLDFFPNGGE
260 270 280 290 300
EMPGCKKNAL SQIVDLDGIW EGTRDFVACN HLRSYKYYSE SILNPDGFAS
310 320 330 340 350
YPCASYRAFE SNKCFPCPDQ GCPQMGHYAD KFAVKTSDET QKYFLNTGDS
360 370 380 390 400
SNFARWRYGV SITLSGKRAT GQAKVALFGS KGNTHQFNIF KGILKPGSTH
410 420 430 440 450
SNEFDAKLDV GTIEKVKFLW NNNVVNPTFP KVGAAKITVQ KGEEKTVHSF
460
CSESTVREDV LLTLTPC
Length:467
Mass (Da):51,482
Last modified:November 1, 1990 - v2
Checksum:i5B186845404E5B5C
GO

Sequence cautioni

The sequence AAA30840 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92I → T in AAA30885 (PubMed:3562437).Curated1
Sequence conflicti98D → N in AAA30885 (PubMed:3562437).Curated1
Sequence conflicti265D → N in AAA30885 (PubMed:3562437).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35302 mRNA. Translation: AAA30885.1.
M28151
, M28141, M28142, M28143, M28145, M28148, M28147, M28146, M28144, M28149, M28150 Genomic DNA. Translation: AAA30840.1. Sequence problems.
PIRiB24392. LIDG.
RefSeqiNP_001003319.2. NM_001003319.2.
UniGeneiCfa.3881.

Genome annotation databases

EnsembliENSCAFT00000018834; ENSCAFP00000017453; ENSCAFG00000011852.
GeneIDi404010.
KEGGicfa:404010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35302 mRNA. Translation: AAA30885.1.
M28151
, M28141, M28142, M28143, M28145, M28148, M28147, M28146, M28144, M28149, M28150 Genomic DNA. Translation: AAA30840.1. Sequence problems.
PIRiB24392. LIDG.
RefSeqiNP_001003319.2. NM_001003319.2.
UniGeneiCfa.3881.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RP1X-ray2.10A18-467[»]
ProteinModelPortaliP06857.
SMRiP06857.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000017453.

Protein family/group databases

ESTHERicanfa-1plip. Pancreatic_lipase.

Proteomic databases

PaxDbiP06857.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000018834; ENSCAFP00000017453; ENSCAFG00000011852.
GeneIDi404010.
KEGGicfa:404010.

Organism-specific databases

CTDi5407.

Phylogenomic databases

eggNOGiENOG410IHRX. Eukaryota.
ENOG410Y92X. LUCA.
GeneTreeiENSGT00760000119069.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiP06857.
KOiK14074.
OMAiKAKEVCY.
OrthoDBiEOG091G0DJ5.
TreeFamiTF324997.

Enzyme and pathway databases

BRENDAi3.1.1.26. 1153.

Miscellaneous databases

EvolutionaryTraceiP06857.

Family and domain databases

CDDicd00707. Pancreat_lipase_like. 1 hit.
Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR013818. Lipase/vitellogenin.
IPR016272. Lipase_LIPH.
IPR033906. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIPR1_CANLF
AccessioniPrimary (citable) accession number: P06857
Secondary accession number(s): Q28287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1990
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally (PubMed:3562437 and PubMed:2502543) thought to be the pancreatic lipase, but has been shown to lack lipase activity.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.