Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P06855

- KIPN_BPT4

UniProt

P06855 - KIPN_BPT4

Protein

Polynucleotide kinase

Gene

pseT

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Acts as a 5'-hydroxyl kinase, a 3'-phosphatase and a 2',3'-cyclic phosphodiesterase. Catalyzes the transfer of the terminal phosphate of ATP to the 5'-hydroxyl termini of ribo- and deoxyribonucleotides. In the presence of ADP the enzyme also catalyzes an exchange reaction. In the exchange reaction, an excess ADP causes the enzyme to transfer the 5' terminal phosphate from phosphorylated DNA to ADP. These activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase.

    Catalytic activityi

    ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. viral process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKP06855.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polynucleotide kinase (EC:2.7.1.78)
    Short name:
    PNK
    Alternative name(s):
    Polynucleotide 5'-hydroxyl-kinase
    Gene namesi
    Name:pseT
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 301301Polynucleotide kinasePRO_0000164949Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    Secondary structure

    1
    301
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86
    Helixi15 – 2511
    Beta strandi29 – 324
    Helixi34 – 418
    Helixi47 – 493
    Helixi54 – 7219
    Helixi75 – 773
    Beta strandi80 – 834
    Helixi90 – 10314
    Beta strandi106 – 1116
    Helixi116 – 1238
    Helixi127 – 1293
    Helixi133 – 14715
    Beta strandi156 – 1583
    Beta strandi160 – 1656
    Turni168 – 1703
    Helixi181 – 1866
    Helixi191 – 20212
    Beta strandi206 – 2116
    Beta strandi218 – 2203
    Helixi223 – 23412
    Beta strandi241 – 2455
    Helixi254 – 26512
    Turni266 – 2694
    Beta strandi271 – 2777
    Helixi280 – 2889
    Beta strandi293 – 2953

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LTQX-ray2.33A1-301[»]
    1LY1X-ray2.00A1-181[»]
    1RC8X-ray2.75A1-301[»]
    1RPZX-ray2.90A1-301[»]
    1RRCX-ray2.46A1-301[»]
    2IA5X-ray2.90A/B/C/D/E/F/G/H/I/J/K/L1-301[»]
    ProteinModelPortaliP06855.
    SMRiP06855. Positions 1-300.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP06855.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR023214. HAD-like_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF56784. SSF56784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P06855-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKIILTIGC PGSGKSTWAR EFIAKNPGFY NINRDDYRQS IMAHEERDEY    50
    KYTKKKEGIV TGMQFDTAKS ILYGGDSVKG VIISDTNLNP ERRLAWETFA 100
    KEYGWKVEHK VFDVPWTELV KRNSKRGTKA VPIDVLRSMY KSMREYLGLP 150
    VYNGTPGKPK AVIFDVDGTL AKMNGRGPYD LEKCDTDVIN PMVVELSKMY 200
    ALMGYQIVVV SGRESGTKED PTKYYRMTRK WVEDIAGVPL VMQCQREQGD 250
    TRKDDVVKEE IFWKHIAPHF DVKLAIDDRT QVVEMWRRIG VECWQVASGD 300
    F 301
    Length:301
    Mass (Da):34,620
    Last modified:January 1, 1988 - v1
    Checksum:i0EAFB6BA83236D31
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03007 Genomic DNA. Translation: CAA26792.1.
    AF158101 Genomic DNA. Translation: AAD42642.1.
    PIRiA24642. KIBPP4.
    RefSeqiNP_049834.1. NC_000866.4.

    Genome annotation databases

    GeneIDi1258796.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03007 Genomic DNA. Translation: CAA26792.1 .
    AF158101 Genomic DNA. Translation: AAD42642.1 .
    PIRi A24642. KIBPP4.
    RefSeqi NP_049834.1. NC_000866.4.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LTQ X-ray 2.33 A 1-301 [» ]
    1LY1 X-ray 2.00 A 1-181 [» ]
    1RC8 X-ray 2.75 A 1-301 [» ]
    1RPZ X-ray 2.90 A 1-301 [» ]
    1RRC X-ray 2.46 A 1-301 [» ]
    2IA5 X-ray 2.90 A/B/C/D/E/F/G/H/I/J/K/L 1-301 [» ]
    ProteinModelPortali P06855.
    SMRi P06855. Positions 1-300.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P06855.
    ChEMBLi CHEMBL5292.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258796.

    Enzyme and pathway databases

    SABIO-RK P06855.

    Miscellaneous databases

    EvolutionaryTracei P06855.

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR023214. HAD-like_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF56784. SSF56784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "T4 polynucleotide kinase; cloning of the gene (pseT) and amplification of its product."
      Midgley C.A., Murray N.E.
      EMBO J. 4:2695-2703(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase."
      Galburt E.A., Pelletier J., Wilson G., Stoddard B.L.
      Structure 10:1249-1260(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.33 ANGSTROMS).

    Entry informationi

    Entry nameiKIPN_BPT4
    AccessioniPrimary (citable) accession number: P06855
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3