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P06855

- KIPN_BPT4

UniProt

P06855 - KIPN_BPT4

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Protein

Polynucleotide kinase

Gene
pseT
Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a 5'-hydroxyl kinase, a 3'-phosphatase and a 2',3'-cyclic phosphodiesterase. Catalyzes the transfer of the terminal phosphate of ATP to the 5'-hydroxyl termini of ribo- and deoxyribonucleotides. In the presence of ADP the enzyme also catalyzes an exchange reaction. In the exchange reaction, an excess ADP causes the enzyme to transfer the 5' terminal phosphate from phosphorylated DNA to ADP. These activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase.

Catalytic activityi

ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP06855.

Names & Taxonomyi

Protein namesi
Recommended name:
Polynucleotide kinase (EC:2.7.1.78)
Short name:
PNK
Alternative name(s):
Polynucleotide 5'-hydroxyl-kinase
Gene namesi
Name:pseT
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087: Genome

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Polynucleotide kinasePRO_0000164949Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1
301
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86
Helixi15 – 2511
Beta strandi29 – 324
Helixi34 – 418
Helixi47 – 493
Helixi54 – 7219
Helixi75 – 773
Beta strandi80 – 834
Helixi90 – 10314
Beta strandi106 – 1116
Helixi116 – 1238
Helixi127 – 1293
Helixi133 – 14715
Beta strandi156 – 1583
Beta strandi160 – 1656
Turni168 – 1703
Helixi181 – 1866
Helixi191 – 20212
Beta strandi206 – 2116
Beta strandi218 – 2203
Helixi223 – 23412
Beta strandi241 – 2455
Helixi254 – 26512
Turni266 – 2694
Beta strandi271 – 2777
Helixi280 – 2889
Beta strandi293 – 2953

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LTQX-ray2.33A1-301[»]
1LY1X-ray2.00A1-181[»]
1RC8X-ray2.75A1-301[»]
1RPZX-ray2.90A1-301[»]
1RRCX-ray2.46A1-301[»]
2IA5X-ray2.90A/B/C/D/E/F/G/H/I/J/K/L1-301[»]
ProteinModelPortaliP06855.
SMRiP06855. Positions 1-300.

Miscellaneous databases

EvolutionaryTraceiP06855.

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF56784. SSF56784. 1 hit.

Sequencei

Sequence statusi: Complete.

P06855-1 [UniParc]FASTAAdd to Basket

« Hide

MKKIILTIGC PGSGKSTWAR EFIAKNPGFY NINRDDYRQS IMAHEERDEY    50
KYTKKKEGIV TGMQFDTAKS ILYGGDSVKG VIISDTNLNP ERRLAWETFA 100
KEYGWKVEHK VFDVPWTELV KRNSKRGTKA VPIDVLRSMY KSMREYLGLP 150
VYNGTPGKPK AVIFDVDGTL AKMNGRGPYD LEKCDTDVIN PMVVELSKMY 200
ALMGYQIVVV SGRESGTKED PTKYYRMTRK WVEDIAGVPL VMQCQREQGD 250
TRKDDVVKEE IFWKHIAPHF DVKLAIDDRT QVVEMWRRIG VECWQVASGD 300
F 301
Length:301
Mass (Da):34,620
Last modified:January 1, 1988 - v1
Checksum:i0EAFB6BA83236D31
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03007 Genomic DNA. Translation: CAA26792.1.
AF158101 Genomic DNA. Translation: AAD42642.1.
PIRiA24642. KIBPP4.
RefSeqiNP_049834.1. NC_000866.4.

Genome annotation databases

GeneIDi1258796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03007 Genomic DNA. Translation: CAA26792.1 .
AF158101 Genomic DNA. Translation: AAD42642.1 .
PIRi A24642. KIBPP4.
RefSeqi NP_049834.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1LTQ X-ray 2.33 A 1-301 [» ]
1LY1 X-ray 2.00 A 1-181 [» ]
1RC8 X-ray 2.75 A 1-301 [» ]
1RPZ X-ray 2.90 A 1-301 [» ]
1RRC X-ray 2.46 A 1-301 [» ]
2IA5 X-ray 2.90 A/B/C/D/E/F/G/H/I/J/K/L 1-301 [» ]
ProteinModelPortali P06855.
SMRi P06855. Positions 1-300.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P06855.
ChEMBLi CHEMBL5292.
DrugBanki DB01093. Dimethyl sulfoxide.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1258796.

Enzyme and pathway databases

SABIO-RK P06855.

Miscellaneous databases

EvolutionaryTracei P06855.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR023214. HAD-like_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF56784. SSF56784. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "T4 polynucleotide kinase; cloning of the gene (pseT) and amplification of its product."
    Midgley C.A., Murray N.E.
    EMBO J. 4:2695-2703(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase."
    Galburt E.A., Pelletier J., Wilson G., Stoddard B.L.
    Structure 10:1249-1260(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.33 ANGSTROMS).

Entry informationi

Entry nameiKIPN_BPT4
AccessioniPrimary (citable) accession number: P06855
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 3, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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