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Reviewed, UniProtKB/Swiss-Prot P06855 (KIPN_BPT4)

Last modified June 16, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Polynucleotide kinase
      Short name=PNK
    EC=2.7.1.78
Alternative name(s):
    Polynucleotide 5'-hydroxyl-kinase
Gene names
Name: pseT
OrganismEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifier10665 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeT4-like viruses
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts as a 5'-hydroxyl kinase, a 3'-phosphatase and a 2',3'-cyclic phosphodiesterase. Catalyzes the transfer of the terminal phosphate of ATP to the 5'-hydroxyl termini of ribo- and deoxyribonucleotides. In the presence of ADP the enzyme also catalyzes an exchange reaction. In the exchange reaction, an excess ADP causes the enzyme to transfer the 5' terminal phosphate from phosphorylated DNA to ADP. These activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase.

Catalytic activity

ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.

Subunit structure

Homotetramer.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical term3D-structure
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301Polynucleotide kinase
PRO_0000164949

Secondary structure

................................................ 301
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06855-1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 0EAFB6BA83236D31

FASTA30134,620
        10         20         30         40         50         60 
MKKIILTIGC PGSGKSTWAR EFIAKNPGFY NINRDDYRQS IMAHEERDEY KYTKKKEGIV 

        70         80         90        100        110        120 
TGMQFDTAKS ILYGGDSVKG VIISDTNLNP ERRLAWETFA KEYGWKVEHK VFDVPWTELV 

       130        140        150        160        170        180 
KRNSKRGTKA VPIDVLRSMY KSMREYLGLP VYNGTPGKPK AVIFDVDGTL AKMNGRGPYD 

       190        200        210        220        230        240 
LEKCDTDVIN PMVVELSKMY ALMGYQIVVV SGRESGTKED PTKYYRMTRK WVEDIAGVPL 

       250        260        270        280        290        300 
VMQCQREQGD TRKDDVVKEE IFWKHIAPHF DVKLAIDDRT QVVEMWRRIG VECWQVASGD 


F 

« Hide

References

« Hide 'large scale' references
[1]"T4 polynucleotide kinase; cloning of the gene (pseT) and amplification of its product."
Midgley C.A., Murray N.E.
EMBO J. 4:2695-2703(1985) [PubMed: 2996886] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Bacteriophage T4 genome."
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.
Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed: 12626685] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase."
Galburt E.A., Pelletier J., Wilson G., Stoddard B.L.
Structure 10:1249-1260(2002) [PubMed: 12220496] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.33 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

X03007 Genomic DNA. Translation: CAA26792.1.
AF158101 Genomic DNA. Translation: AAD42642.1.
PIRKIBPP4. A24642.
RefSeqNP_049834.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1LTQX-ray2.33A1-301[»]
1LY1X-ray2.00A1-181[»]
1RC8X-ray2.75A1-301[»]
1RPZX-ray2.90A1-301[»]
1RRCX-ray2.46A1-301[»]
2IA5X-ray2.90A/B/C/D/E/F/G/H/I/J/K/L1-301[»]
ModBaseSearch...

Genome annotation databases

GeneID1258796.
GenomeReviewsGene locus T4p224 in contig AF158101_GR.

Enzyme and pathway databases

BRENDA2.7.1.78. 1108.

Family and domain databases

ProtoNetSearch...

Other Resources

DrugBankDB01093. Dimethyl sulfoxide.

Entry information

Entry nameKIPN_BPT4
AccessionPrimary (citable) accession number: P06855
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents