Reviewed, UniProtKB/Swiss-Prot P06850 (CRF_HUMAN)

Names and origin

Protein names

Recommended name:
    Corticoliberin
Alternative name(s):
    Corticotropin-releasing hormone
    Corticotropin-releasing factor
      Short name=CRF

Gene names

Name:

CRH

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This hormone from hypothalamus regulates the release of corticotropin from pituitary gland.
Subcellular location	Secreted.
Sequence similarities	Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from Experiment. Source: Reactome female pregnancy Traceable author statement. Source: ProtInc negative regulation of circadian sleep/wake cycle, REM sleep Non-traceable author statement. Source: UniProtKB parturition Traceable author statement. Source: ProtInc positive regulation of circadian sleep/wake cycle, wakefulness Non-traceable author statement. Source: UniProtKB positive regulation of cortisol secretion Inferred from direct assay. Source: UniProtKB synaptic transmission Traceable author statement. Source: ProtInc
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Traceable author statement. Source: ProtInc
Molecular function	neuropeptide hormone activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Probable

Propeptide

25 – 153

129

PRO_0000006212

Peptide

154 – 194

41

Corticoliberin

PRO_0000006213

Amino acid modifications

Modified residue

194

1

Isoleucine amide

Secondary structure

1

.

.

.

196

Helix Strand Turn

Details...

Helix

180 – 193

14

Sequences

	Sequence		Length	Mass (Da)
	P06850-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 0CCDFF05BE364E92	FASTA	196	21,422
	10 20 30 40 50 60 MRLPLLVSAG VLLVALLPCP PCRALLSRGP VPGARQAPQH PQPLDFFQPP PQSEQPQQPQ 70 80 90 100 110 120 ARPVLLRMGE EYFLRLGNLN KSPAAPLSPA SSLLAGGSGS RPSPEQATAN FFRVLLQQLL 130 140 150 160 170 180 LPRRSLDSPA ALAERGARNA LGGHQEAPER ERRSEEPPIS LDLTFHLLRE VLEMARAEQL 190 AQQAHSNRKL MEIIGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and sequence analysis of the human corticotropin-releasing factor precursor gene." Shibahara S., Morimoto Y., Furutani Y., Notake M., Takahashi H., Shimizu S., Horikawa S., Numa S. EMBO J. 2:775-779(1983) [PubMed: 6605851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Preprocorticotropin releasing hormone: cDNA sequence and in vitro processing." Robinson B.G., D'Angio L.A. Jr., Pasieka K.B., Majzoub J.A. Mol. Cell. Endocrinol. 61:175-180(1989) [PubMed: 2783917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed: 16303743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	"Isolation and characterization of a corticotropin-releasing hormone-like peptide from human placenta." Sasaki A., Tempst P., Liotta A.S., Margioris A.N., Hood L.E., Kent S.B., Sato S., Shinkawa O., Yoshinaga K., Krieger D.T. J. Clin. Endocrinol. Metab. 67:768-773(1988) [PubMed: 3262120] [Abstract] Cited for: PROTEIN SEQUENCE OF 154-186.
[8]	"Solution structure of human corticotropin releasing factor by 1H NMR and distance geometry with restrained molecular dynamics." Romier C., Bernassau J.-M., Cambillau C., Darbon H. Protein Eng. 6:149-156(1993) [PubMed: 8386360] [Abstract] Cited for: STRUCTURE BY NMR OF 154-194.
+	Additional computationally mapped references.

Web resources

Wikipedia

Corticotropin-releasing hormone entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00571 Genomic DNA. Translation: CAA23834.1.
BT007453 mRNA. Translation: AAP36121.1.
AK075431 mRNA. Translation: BAG52136.1.
CH471068 Genomic DNA. Translation: EAW86897.1.
BC002599 mRNA. Translation: AAH02599.1.
BC011031 mRNA. Translation: AAH11031.1.

IPI

IPI00027839.

PIR

A30327.

RefSeq

NP_000747.1.

UniGene

Hs.75294

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EHT	X-ray	3.40	B	180-194	[»]
3EHU	X-ray	1.96	C/D	175-194	[»]

SMR

P06850. Positions 154-194.

ModBase

Search...

Protein-protein interaction databases

STRING

P06850.

Proteomic databases

PRIDE

P06850.

Genome annotation databases

Ensembl

ENST00000276571; ENSP00000276571; ENSG00000147571; Homo sapiens. [Genome view]

GeneID

1392.

KEGG

hsa:1392.

UCSC

uc003xvy.1. human.

Organism-specific databases

CTD

1392.

GeneCards

GC08M067251.

H-InvDB

HIX0007552.

HGNC

HGNC:2355. CRH.

MIM

122560. gene+phenotype.

PharmGKB

PA119.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21212.

HOGENOM

HBG715743.

HOVERGEN

P06850.

InParanoid

P06850.

OMA

AANFFRV.

OrthoDB

EOG9GB9RN.

PhylomeDB

P06850.

Enzyme and pathway databases

Reactome

REACT_14797. Signaling by GPCR.

Gene expression databases

ArrayExpress

P06850.

Bgee

P06850.

CleanEx

HS_CRH.

Genevestigator

P06850.

GermOnline

ENSG00000147571. Homo sapiens.

Family and domain databases

InterPro

IPR018447. Corticotropin-releasing_fac_C.
IPR018446. Corticotropin-releasing_fac_CS.
IPR000187. Corticotropin-releasing_factor.
IPR003620. Urocortin_CRF.
[Graphical view]

PANTHER

PTHR15035. Urocortin_CRF. 1 hit.

Pfam

PF00473. CRF. 1 hit.
[Graphical view]

PRINTS

PR01612. CRFFAMILY.

SMART

SM00039. CRF. 1 hit.
[Graphical view]

PROSITE

PS00511. CRF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB01285. Corticotropin.

NextBio

5695.

SOURCE

Search...

Entry information

Entry name

CRF_HUMAN

Accession

Primary (citable) accession number: P06850
Secondary accession number(s): B3KQS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	January 19, 2010
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.