Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Replication-associated protein A

Gene

C1

Organism
Wheat dwarf virus (isolate Sweden) (WDV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in enhancement of V-sense gene expression. Acts a an inhibitor of C-sense gene transcription (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi51Divalent metal cationSequence analysis1
Metal bindingi59Divalent metal cationSequence analysis1
Metal bindingi61Divalent metal cationSequence analysis1
Active sitei106For DNA cleavage activityBy similarity1
Metal bindingi110Divalent metal cationSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Endonuclease, Hydrolase, Nuclease, Repressor
Biological processG1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein A (EC:3.1.21.-)
Short name:
RepA
Gene namesi
ORF Names:C1
OrganismiWheat dwarf virus (isolate Sweden) (WDV)
Taxonomic identifieri268789 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeMastrevirus
Virus hostiAvena sativa (Oat) [TaxID: 4498]
Hordeum vulgare (Barley) [TaxID: 4513]
Lolium multiflorum (Italian ryegrass) (Lolium perenne subsp. multiflorum) [TaxID: 4521]
Secale cereale (Rye) [TaxID: 4550]
Triticum aestivum (Wheat) [TaxID: 4565]
Proteomesi
  • UP000007629 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi196C → G: Loss of interaction with the human retinoblastoma-like protein 2. 1 Publication1
Mutagenesisi198E → K: Loss of interaction with the human retinoblastoma-like protein 2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002222201 – 264Replication-associated protein AAdd BLAST264

Interactioni

Subunit structurei

Homooligomer. Interacts (via LXCXE domain) with host retinoblastoma-related protein 1 (RBR1), and may thereby deregulate the host cell cycle. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription.2 Publications

Structurei

3D structure databases

ProteinModelPortaliP06847.
SMRiP06847.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni173 – 185OligomerizationBy similarityAdd BLAST13

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi17 – 21RCR-15
Motifi59 – 64RCR-26
Motifi106 – 109RCR-34
Motifi194 – 198LXCXE motif, interaction with host RBR1Curated5

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 may be involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.
The LXCXE motif specifically binds to host RBR1.

Sequence similaritiesi

Belongs to the geminiviridae Rep protein family.Curated

Phylogenomic databases

OrthoDBiVOG090000BW.

Family and domain databases

InterProiView protein in InterPro
IPR001301. Gemini_AL1_CLV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
PfamiView protein in Pfam
PF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
PRINTSiPR00227. GEMCOATAL1.
PR00228. GEMCOATCLVL1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RepA (identifier: P06847-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSAPRFR VYSKYLFLTY PECTLEPQYA LDSLRTLLNK YEPLYIAAVR
60 70 80 90 100
ELHEDGSPHL HVLVQNKLRA SITNPNALNL RMDTSPFSIF HPNIQAAKDC
110 120 130 140 150
NQVRDYITKE VDSDVNTAEW GTFVAVSTPG RKDRDADMKQ IIESSSSREE
160 170 180 190 200
FLSMVCNRFP FEWSIRLKDF EYTARHLFPD PVATYTPEFP TESLICHETI
210 220 230 240 250
ESWKNEHLYS VSLESYILCT STPADQAQSD LEWMDDYSRS HRGGISPSTS
260
AGQPEQERLP GQGL
Note: Produced from the unspliced transcript.
Length:264
Mass (Da):30,157
Last modified:January 1, 1988 - v1
Checksum:i8137A357E6503A7E
GO
Isoform Rep (identifier: Q67622-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q67622.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:351
Mass (Da):40,511
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02869 Genomic DNA. Translation: CAA26625.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02869 Genomic DNA. Translation: CAA26625.1.

3D structure databases

ProteinModelPortaliP06847.
SMRiP06847.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090000BW.

Family and domain databases

InterProiView protein in InterPro
IPR001301. Gemini_AL1_CLV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
PfamiView protein in Pfam
PF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
PRINTSiPR00227. GEMCOATAL1.
PR00228. GEMCOATCLVL1.
ProtoNetiSearch...

Entry informationi

Entry nameiREPA_WDVS
AccessioniPrimary (citable) accession number: P06847
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: March 15, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.