P06846 (EBGR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator ebgR

Alternative name(s):
Ebg operon repressor

Gene names

Name:	ebgR
Ordered Locus Names:	b3075, JW3046

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Repressor for beta galactosidase alpha and beta subunits (ebgA and ebgC). Binds lactose as an inducer.
Sequence similarities	Contains 1 HTH lacI-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	HTH-type transcriptional regulator ebgR		PRO_0000107943
Regions
	Domain	1 – 57	57	HTH lacI-type
	DNA binding	4 – 23	20	H-T-H motif By similarity
Natural variations
	Natural variant	190	1	D → E Broaden the specificity of inducer recognition.
	Natural variant	195	1	A → T Broaden the specificity of inducer recognition.
	Natural variant	196	1	F → C Broaden the specificity of inducer recognition.

Sequences

	Sequence		Length	Mass (Da)
	P06846 [UniParc]. Last modified March 1, 1992. Version 2. Checksum: 2A8688B46A00EE03	FASTA	327	36,210
	10 20 30 40 50 60 MATLKDIAIE AGVSLATVSR VLNDDPTLNV KEETKHRILE IAEKLEYKTS SARKLQTGAV 70 80 90 100 110 120 NQHHILAIYS YQQELEINDP YYLAIRHGIE TQCEKLGIEL TNCYEHSGLP DIKNVTGILI 130 140 150 160 170 180 VGKPTPALRA AASALTDNIC FIDFHEPGSG YDAVDIDLAR ISKEIIDFYI NQGVNRIGFI 190 200 210 220 230 240 GGEDEPGKAD IREVAFAEYG RLKQVVREED IWRGGFSSSS GYELAKQMLA REDYPKALFV 250 260 270 280 290 300 ASDSIAIGVL RAIHERGLNI PQDISLISVN DIPTARFTFP PLSTVRIHSE MMGSQGVNLV 310 320 YEKARDGRAL PLLVFVPSKL KLRGTTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of artificially evolved ebg enzyme and ebg repressor genes." Hall B.G., Betts P.W., Wootton J.C. Genetics 123:635-648(1989) [PubMed: 2515108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of the ebgR gene of Escherichia coli: evidence that the EBG and LAC operons are descended from a common ancestor." Stokes H.W., Hall B.G. Mol. Biol. Evol. 2:478-483(1985) [PubMed: 3939708] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64441 Genomic DNA. Translation: AAA61970.1. X52031 Genomic DNA. Translation: CAA36273.1. U18997 Genomic DNA. Translation: AAA57876.1. U00096 Genomic DNA. Translation: AAC76110.1. AP009048 Genomic DNA. Translation: BAE77125.1.
PIR	RPECEG. A25752.
RefSeq	NP_417546.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P06846.
SMR	P06846. Positions 2-327.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9488N.
IntAct	P06846. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004926; EBESCP00000004926; EBESCG00000004019. EBESCT00000015159; EBESCP00000014450; EBESCG00000014219.
GeneID	947586.
GenomeReviews	Gene locus JW3046 in contig AP009048_GR. Gene locus b3075 in contig U00096_GR.
KEGG	ecj:JW3046. eco:b3075.
PATRIC	32121566. VBIEscCol129921_3169.
Organism-specific databases
EchoBASE	EB0250.
EcoGene	EG10254. ebgR.
Phylogenomic databases
eggNOG	COG1609.
GeneTree	EBGT00050000009241.
HOGENOM	HBG753640.
OMA	LAQEDYP.
PhylomeDB	P06846.
ProtClustDB	PRK10339.
Enzyme and pathway databases
BioCyc	EcoCyc:PD03254.
Gene expression databases
Genevestigator	P06846.
Family and domain databases
InterPro	IPR010982. Lambda_DNA-bd. IPR001761. Peripla_BP_Lac1. IPR000843. Tscrpt_reg_HTH_LacI. [Graphical view]
Gene3D	G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit.
KO	K12113.
Pfam	PF00356. LacI. 1 hit. PF00532. Peripla_BP_1. 1 hit. [Graphical view]
PRINTS	PR00036. HTHLACI.
SMART	SM00354. HTH_LACI. 1 hit. [Graphical view]
SUPFAM	SSF47413. Lambda_like_DNA. 1 hit.
PROSITE	PS00356. HTH_LACI_1. 1 hit. PS50932. HTH_LACI_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBGR_ECOLI

Accession

Primary (citable) accession number: P06846
Secondary accession number(s): Q2M9D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	March 1, 1992
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program