P06845 (TYRO_STRGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosinase
EC=1.14.18.1

Alternative name(s):
Monophenol monooxygenase

Gene names

Name:	melC2
Synonyms:	mel

Organism

Streptomyces glaucescens

Taxonomic identifier

1907 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.
Catalytic activity	2 L-dopa + O2 = 2 dopaquinone + 2 H2O. L-tyrosine + O2 = dopaquinone + H2O.
Cofactor	Binds 2 copper ions per subunit.
Miscellaneous	The extra- and intra-cellular tyrosinases are identical.
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Biological process	Melanin biosynthesis
Ligand	Copper Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	melanin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW monophenol monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 274	273	Tyrosinase		PRO_0000186738
Sites
	Metal binding	38	1	Copper A
	Metal binding	54	1	Copper A
	Metal binding	63	1	Copper A
	Metal binding	190	1	Copper B
	Metal binding	194	1	Copper B
	Metal binding	216	1	Copper B
Experimental info
	Mutagenesis	38	1	H → Q: Loss of activity.
	Mutagenesis	54	1	H → Q: Loss of activity.
	Mutagenesis	63	1	H → N: Loss of activity.
	Mutagenesis	190	1	H → N: Loss of activity.
	Mutagenesis	191	1	N → Q: Loss of activity.
	Mutagenesis	194	1	H → Q: Loss of activity.
	Mutagenesis	216	1	H → Q: Loss of activity.
	Sequence conflict	216	1	H → N in AAA26834. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P06845 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0FDC67DC2739AA79	FASTA	274	30,874
	10 20 30 40 50 60 MTVRKNQATL TADEKRRFVA AVLELKRSGR YDEFVTTHNA FIIGDTDAGE RTGHRSPSFL 70 80 90 100 110 120 PWHRRYLLEF ERALQSVDAS VALPYWDWSA DRTARASLWA PDFLGGTGRS LDGRVMDGPF 130 140 150 160 170 180 AASAGNWPIN VRVDGRAYLR RSLGTAVREL PTRAEVESVL GMATYDTAPW NSASDGFRNH 190 200 210 220 230 240 LEGWRGVNLH NRVHVWVGGQ MATGMSPNDP VFWLHHAYVD KLWAEWQRRH PGSGYLPAAG 250 260 270 TPDVVDLNDR MKPWNDTSPA DLLDHTAHYT FDTD

References

[1]	"Primary structure of tyrosinase from Streptomyces glaucescens." Huber M., Hintermann G., Lerch K. Biochemistry 24:6038-6044(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: DSM 40716 / ETH 22794 / GLA.0.
[2]	"Identification of two histidines as copper ligands in Streptomyces glaucescens tyrosinase." Huber M., Lerch K. Biochemistry 27:5610-5615(1988) [PubMed] [Europe PMC] [Abstract] Cited for: COPPER-LIGANDS, MUTAGENESIS. Strain: DSM 40716 / ETH 22794 / GLA.0.
[3]	"Albino mutants of Streptomyces glaucescens tyrosinase." Jackman M.P., Hajnal A., Lerch K. Biochem. J. 274:707-713(1991) [PubMed] [Europe PMC] [Abstract] Cited for: COPPER-LIGANDS, MUTAGENESIS. Strain: DSM 40716 / ETH 22794 / GLA.0.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11302 Genomic DNA. Translation: AAA26834.1.
PIR	A24089.
3D structure databases
ProteinModelPortal	P06845.
SMR	P06845. Positions 2-272.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1280.10. 1 hit.
InterPro	IPR002227. Tyrosinase. IPR008922. Unchr_di-copper_centre. [Graphical view]
Pfam	PF00264. Tyrosinase. 1 hit. [Graphical view]
PRINTS	PR00092. TYROSINASE.
SUPFAM	SSF48056. Di-copper_centre. 1 hit.
PROSITE	PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYRO_STRGA

Accession

Primary (citable) accession number: P06845

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program