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Protein

Protein SPT3

Gene

SPT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SPT3 is required for recruitment of TATA-binding protein (TBP) to SAGA-dependent promoters. During SAGA-mediated transcriptional inhibition, SPT3 and SPT8 prevent binding of TBP to the TATA box. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. SPT factors 3, 7 and 8 are required for the initiation of Ty transcription from the delta promoter. SPT3 regulates Ty1 as well as the mating factor genes.5 Publications

Miscellaneous

Present with 1710 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • chromatin organization Source: SGD
  • histone acetylation Source: SGD
  • invasive growth in response to glucose limitation Source: SGD
  • pseudohyphal growth Source: SGD
  • regulation of transcription by RNA polymerase II Source: GO_Central
  • transcription by RNA polymerase II Source: SGD

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29940-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SPT3
Alternative name(s):
Positive regulator of Ty transcription
Gene namesi
Name:SPT3
Ordered Locus Names:YDR392W
ORF Names:D9509.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR392W
SGDiS000002800 SPT3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721661 – 337Protein SPT3Add BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei270PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06844
PaxDbiP06844
PRIDEiP06844

PTM databases

iPTMnetiP06844

Interactioni

Subunit structurei

Component of the 1.8 MDa SAGA complex, which consists of at least of TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA. SPT3 interacts directly with TBP (TATA-binding protein). Component of the SALSA complex, which consists of at least TRA1, SPT7 (C-terminal truncated form), TAF5, ADA3, SPT20, TAF12, TAF6, HFI1, GCN5, ADA2 and SPT3. Component of the SLIK complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SPT20, RTG2, TAF12, TAF6, HFI1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10 and TAF9.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32454, 482 interactors
DIPiDIP-2204N
IntActiP06844, 31 interactors
MINTiP06844
STRINGi4932.YDR392W

Structurei

3D structure databases

ProteinModelPortaliP06844
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SPT3 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010738
HOGENOMiHOG000205281
InParanoidiP06844
KOiK11313
OMAiLQFMFNE
OrthoDBiEOG092C3CUK

Family and domain databases

CDDicd07978 TAF13, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR003195 TFIID_TAF13
PANTHERiPTHR11380 PTHR11380, 1 hit
PfamiView protein in Pfam
PF02269 TFIID-18kDa, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit

Sequencei

Sequence statusi: Complete.

P06844-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDKHKYRVE IQQMMFVSGE INDPPVETTS LIEDIVRGQV IEILLQSNKT
60 70 80 90 100
AHLRGSRSIL PEDVIFLIRH DKAKVNRLRT YLSWKDLRKN AKDQDASAGV
110 120 130 140 150
ASGTGNPGAG GEDDLKKAGG GEKDEKDGGN MMKVKKSQIK LPWELQFMFN
160 170 180 190 200
EHPLENNDDN DDMDEDEREA NIVTLKRLKM ADDRTRNMTK EEYVHWSDCR
210 220 230 240 250
QASFTFRKNK RFKDWSGISQ LTEGKPHDDV IDILGFLTFE IVCSLTETAL
260 270 280 290 300
KIKQREQVLQ TQKDKSQQSS QDNTNFEFAS STLHRKKRLF DGPENVINPL
310 320 330
KPRHIEEAWR VLQTIDMRHR ALTNFKGGRL SSKPIIM
Length:337
Mass (Da):38,800
Last modified:January 1, 1988 - v1
Checksum:iE0F60B61AB155C17
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti76N → K in strain: CLIB 556 and CLIB 630. 1 Publication1
Natural varianti93D → N in strain: CLIB 556 and CLIB 630. 1 Publication1
Natural varianti120G → S in strain: CLIB 413 haplotype Ha2. 1 Publication1
Natural varianti134V → I in strain: R13 haplotype Ha2. 1 Publication1
Natural varianti318R → K in strain: YIIc12 haplotype Ha2 and YIIc17. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04383 Genomic DNA Translation: CAA27970.1
AJ585583 Genomic DNA Translation: CAE52103.1
AJ585584 Genomic DNA Translation: CAE52104.1
AJ585585 Genomic DNA Translation: CAE52105.1
AJ585586 Genomic DNA Translation: CAE52106.1
AJ585587 Genomic DNA Translation: CAE52107.1
AJ585588 Genomic DNA Translation: CAE52108.1
AJ585589 Genomic DNA Translation: CAE52109.1
AJ585590 Genomic DNA Translation: CAE52110.1
AJ585591 Genomic DNA Translation: CAE52111.1
AJ585592 Genomic DNA Translation: CAE52112.1
AJ585593 Genomic DNA Translation: CAE52113.1
AJ585594 Genomic DNA Translation: CAE52114.1
AJ585595 Genomic DNA Translation: CAE52115.1
AJ585596 Genomic DNA Translation: CAE52116.1
AJ585597 Genomic DNA Translation: CAE52117.1
AJ585598 Genomic DNA Translation: CAE52118.1
AJ585599 Genomic DNA Translation: CAE52119.1
U32274 Genomic DNA Translation: AAB64834.1
BK006938 Genomic DNA Translation: DAA12236.1
PIRiA24330
RefSeqiNP_010680.1, NM_001180700.1

Genome annotation databases

EnsemblFungiiYDR392W; YDR392W; YDR392W
GeneIDi852001
KEGGisce:YDR392W

Similar proteinsi

Entry informationi

Entry nameiSPT3_YEAST
AccessioniPrimary (citable) accession number: P06844
Secondary accession number(s): D6VT26
, Q70DE7, Q70DE9, Q70DF1, Q70DF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: March 28, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health