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Protein

Protein SPT2

Gene

SPT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone chaperone that stabilizes pre-existing histone tetramers and regulates replication-independent histone exchange on chromatin (PubMed:26109053). Required for normal chromatin refolding in the coding region of transcribed genes, and for the suppression of spurious transcription (PubMed:26109053). Global regulatory protein that plays positive as well as negative regulatory roles in transcription (PubMed:2072912).2 Publications

GO - Molecular functioni

  • DNA binding Source: SGD
  • DNA secondary structure binding Source: SGD
  • histone binding Source: UniProtKB

GO - Biological processi

  • chromatin organization Source: SGD
  • histone exchange Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of chromatin assembly Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • RNA polyadenylation Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30322-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SPT2
Alternative name(s):
Negative regulator of Ty transcription
Gene namesi
Name:SPT2
Synonyms:SIN12 Publications, SPM2
Ordered Locus Names:YER161C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER161C.
SGDiS000000963. SPT2.

Subcellular locationi

  • Nucleus 2 Publications

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi289 – 2902ME → AA: Strongly reduces histone binding. 1 Publication
Mutagenesisi299 – 3002EE → AA: Strongly reduces histone binding. 1 Publication
Mutagenesisi310 – 3112ED → AA: Strongly reduces histone binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 333332Protein SPT2PRO_0000072163Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei126 – 1261PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP06843.

PTM databases

iPTMnetiP06843.

Interactioni

Subunit structurei

Interacts with tetramers formed by histone H3 and H4 (PubMed:26109053). Interacts with SAP1 and CDC23 (PubMed:8654588, PubMed:8710860). Component of the SAGA complex, a large multiprotein complex that modifies the chromatin, at least composed of SPT2, SPT7, SPT8, SPT20/ADA5, HFI1/ADA1, ADA2, ADA3/NGG1, TRA1 and GCN5 (PubMed:9885573).4 Publications

GO - Molecular functioni

  • histone binding Source: UniProtKB

Protein-protein interaction databases

BioGridi36914. 183 interactions.
DIPiDIP-1657N.
IntActiP06843. 5 interactions.
MINTiMINT-397095.

Structurei

3D structure databases

ProteinModelPortaliP06843.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni259 – 33375Important for interaction with histones1 PublicationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili218 – 24225Sequence analysisAdd
BLAST
Coiled coili292 – 31625Sequence analysisAdd
BLAST

Domaini

The acidic C-terminal domain mediates interaction with histone H3/H4 complexes.1 Publication

Sequence similaritiesi

Belongs to the SPT2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000141754.
InParanoidiP06843.
KOiK15193.
OMAiMEANEME.
OrthoDBiEOG7J70S8.

Family and domain databases

InterProiIPR013256. Chromatin_SPT2.
[Graphical view]
PfamiPF08243. SPT2. 1 hit.
[Graphical view]
SMARTiSM00784. SPT2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFLSKLSQI RKSTTASKAQ VQDPLPKKND EEYSLLPKNY IRDEDPAVKR
60 70 80 90 100
LKELRRQELL KNGALAKKSG VKRKRGTSSG SEKKKIERND DDEGGLGIRF
110 120 130 140 150
KRSIGASHAP LKPVVRKKPE PIKKMSFEEL MKQAENNEKQ PPKVKSSEPV
160 170 180 190 200
TKERPHFNKP GFKSSKRPQK KASPGATLRG VSSGGNSIKS SDSPKPVKLN
210 220 230 240 250
LPTNGFAQPN RRLKEKLESR KQKSRYQDDY DEEDNDMDDF IEDDEDEGYH
260 270 280 290 300
SKSKHSNGPG YDRDEIWAMF NRGKKRSEYD YDELEDDDME ANEMEILEEE
310 320 330
EMARKMARLE DKREEAWLKK HEEEKRRRKK GIR
Length:333
Mass (Da):38,551
Last modified:January 1, 1988 - v1
Checksum:i60C3BED7EEA99535
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11165 Genomic DNA. Translation: AAA35083.1.
U18917 Genomic DNA. Translation: AAB64688.1.
BK006939 Genomic DNA. Translation: DAA07823.1.
PIRiA23438.
RefSeqiNP_011088.1. NM_001179051.1.

Genome annotation databases

EnsemblFungiiYER161C; YER161C; YER161C.
GeneIDi856908.
KEGGisce:YER161C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11165 Genomic DNA. Translation: AAA35083.1.
U18917 Genomic DNA. Translation: AAB64688.1.
BK006939 Genomic DNA. Translation: DAA07823.1.
PIRiA23438.
RefSeqiNP_011088.1. NM_001179051.1.

3D structure databases

ProteinModelPortaliP06843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36914. 183 interactions.
DIPiDIP-1657N.
IntActiP06843. 5 interactions.
MINTiMINT-397095.

PTM databases

iPTMnetiP06843.

Proteomic databases

MaxQBiP06843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER161C; YER161C; YER161C.
GeneIDi856908.
KEGGisce:YER161C.

Organism-specific databases

EuPathDBiFungiDB:YER161C.
SGDiS000000963. SPT2.

Phylogenomic databases

HOGENOMiHOG000141754.
InParanoidiP06843.
KOiK15193.
OMAiMEANEME.
OrthoDBiEOG7J70S8.

Enzyme and pathway databases

BioCyciYEAST:G3O-30322-MONOMER.

Miscellaneous databases

PROiP06843.

Family and domain databases

InterProiIPR013256. Chromatin_SPT2.
[Graphical view]
PfamiPF08243. SPT2. 1 hit.
[Graphical view]
SMARTiSM00784. SPT2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the SPT2 gene, a negative regulator of Ty-controlled yeast gene expression."
    Roeder G.S., Beard C., Smith M., Keranen S.
    Mol. Cell. Biol. 5:1543-1553(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1."
    Kruger W., Herskowitz I.
    Mol. Cell. Biol. 11:4135-4146(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, DNA-BINDING.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Association of yeast SAP1, a novel member of the 'AAA' ATPase family of proteins, with the chromatin protein SIN1."
    Liberzon A., Shpungin S., Bangio H., Yona E., Katcoff D.J.
    FEBS Lett. 388:5-10(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SAP1.
  6. "Association of yeast SIN1 with the tetratrico peptide repeats of CDC23."
    Shpungin S., Liberzon A., Bangio H., Yona E., Katcoff D.J.
    Proc. Natl. Acad. Sci. U.S.A. 93:8274-8277(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC23.
  7. "The ATM-related cofactor Tra1 is a component of the purified SAGA complex."
    Grant P.A., Schieltz D., Pray-Grant M.G., Yates J.R. III, Workman J.L.
    Mol. Cell 2:863-867(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A SAGA COMPLEX WITH SPT7; HFI1; SPT8; GCN5; SPT20; ADA2; ADA3 AND TRA1.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Structure-function studies of histone H3/H4 tetramer maintenance during transcription by chaperone Spt2."
    Chen S., Rufiange A., Huang H., Rajashankar K.R., Nourani A., Patel D.J.
    Genes Dev. 29:1326-1340(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HISTONE H3 AND H4, DOMAIN, SUBCELLULAR LOCATION, MUTAGENESIS OF 289-MET-GLU-290; 299-GLU-GLU-300 AND 310-GLU-ASP-311.

Entry informationi

Entry nameiSPT2_YEAST
AccessioniPrimary (citable) accession number: P06843
Secondary accession number(s): D3DM69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: July 6, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.