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P06843

- SPT2_YEAST

UniProt

P06843 - SPT2_YEAST

Protein

Protein SPT2

Gene

SPT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Chromatin protein. Global regulatory protein that plays positive as well as negative regulatory roles in transcription.

    GO - Molecular functioni

    1. DNA binding Source: SGD
    2. DNA secondary structure binding Source: SGD

    GO - Biological processi

    1. chromatin organization Source: SGD
    2. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    3. RNA polyadenylation Source: SGD
    4. transcription elongation from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30322-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein SPT2
    Alternative name(s):
    Negative regulator of Ty transcription
    Gene namesi
    Name:SPT2
    Synonyms:SIN1, SPM2
    Ordered Locus Names:YER161C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER161c.
    SGDiS000000963. SPT2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 333332Protein SPT2PRO_0000072163Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei126 – 1261Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP06843.
    PaxDbiP06843.
    PeptideAtlasiP06843.

    Expressioni

    Gene expression databases

    GenevestigatoriP06843.

    Interactioni

    Subunit structurei

    Interacts with SAP1 and CDC23. Component of the SAGA complex, a large multiprotein complex that modifies the chromatin, at least composed of SPT2, SPT7, SPT8, SPT20/ADA5, HFI1/ADA1, ADA2, ADA3/NGG1, TRA1 and GCN5.1 Publication

    Protein-protein interaction databases

    BioGridi36914. 185 interactions.
    DIPiDIP-1657N.
    IntActiP06843. 3 interactions.
    MINTiMINT-397095.
    STRINGi4932.YER161C.

    Structurei

    3D structure databases

    ProteinModelPortaliP06843.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili218 – 24225Sequence AnalysisAdd
    BLAST
    Coiled coili292 – 31625Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SPT2 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG81249.
    HOGENOMiHOG000141754.
    KOiK15193.
    OMAiMEANEME.
    OrthoDBiEOG7J70S8.

    Family and domain databases

    InterProiIPR013256. Chromatin_SPT2.
    [Graphical view]
    PfamiPF08243. SPT2. 1 hit.
    [Graphical view]
    SMARTiSM00784. SPT2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06843-1 [UniParc]FASTAAdd to Basket

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    MSFLSKLSQI RKSTTASKAQ VQDPLPKKND EEYSLLPKNY IRDEDPAVKR    50
    LKELRRQELL KNGALAKKSG VKRKRGTSSG SEKKKIERND DDEGGLGIRF 100
    KRSIGASHAP LKPVVRKKPE PIKKMSFEEL MKQAENNEKQ PPKVKSSEPV 150
    TKERPHFNKP GFKSSKRPQK KASPGATLRG VSSGGNSIKS SDSPKPVKLN 200
    LPTNGFAQPN RRLKEKLESR KQKSRYQDDY DEEDNDMDDF IEDDEDEGYH 250
    SKSKHSNGPG YDRDEIWAMF NRGKKRSEYD YDELEDDDME ANEMEILEEE 300
    EMARKMARLE DKREEAWLKK HEEEKRRRKK GIR 333
    Length:333
    Mass (Da):38,551
    Last modified:January 1, 1988 - v1
    Checksum:i60C3BED7EEA99535
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11165 Genomic DNA. Translation: AAA35083.1.
    U18917 Genomic DNA. Translation: AAB64688.1.
    BK006939 Genomic DNA. Translation: DAA07823.1.
    PIRiA23438.
    RefSeqiNP_011088.1. NM_001179051.1.

    Genome annotation databases

    EnsemblFungiiYER161C; YER161C; YER161C.
    GeneIDi856908.
    KEGGisce:YER161C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11165 Genomic DNA. Translation: AAA35083.1 .
    U18917 Genomic DNA. Translation: AAB64688.1 .
    BK006939 Genomic DNA. Translation: DAA07823.1 .
    PIRi A23438.
    RefSeqi NP_011088.1. NM_001179051.1.

    3D structure databases

    ProteinModelPortali P06843.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36914. 185 interactions.
    DIPi DIP-1657N.
    IntActi P06843. 3 interactions.
    MINTi MINT-397095.
    STRINGi 4932.YER161C.

    Proteomic databases

    MaxQBi P06843.
    PaxDbi P06843.
    PeptideAtlasi P06843.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER161C ; YER161C ; YER161C .
    GeneIDi 856908.
    KEGGi sce:YER161C.

    Organism-specific databases

    CYGDi YER161c.
    SGDi S000000963. SPT2.

    Phylogenomic databases

    eggNOGi NOG81249.
    HOGENOMi HOG000141754.
    KOi K15193.
    OMAi MEANEME.
    OrthoDBi EOG7J70S8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30322-MONOMER.

    Miscellaneous databases

    NextBioi 983344.

    Gene expression databases

    Genevestigatori P06843.

    Family and domain databases

    InterProi IPR013256. Chromatin_SPT2.
    [Graphical view ]
    Pfami PF08243. SPT2. 1 hit.
    [Graphical view ]
    SMARTi SM00784. SPT2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the SPT2 gene, a negative regulator of Ty-controlled yeast gene expression."
      Roeder G.S., Beard C., Smith M., Keranen S.
      Mol. Cell. Biol. 5:1543-1553(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1."
      Kruger W., Herskowitz I.
      Mol. Cell. Biol. 11:4135-4146(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The ATM-related cofactor Tra1 is a component of the purified SAGA complex."
      Grant P.A., Schieltz D., Pray-Grant M.G., Yates J.R. III, Workman J.L.
      Mol. Cell 2:863-867(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A SAGA COMPLEX WITH SPT7; HFI1; SPT8; GCN5; SPT20; ADA2; ADA3 AND TRA1.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSPT2_YEAST
    AccessioniPrimary (citable) accession number: P06843
    Secondary accession number(s): D3DM69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1240 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3