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P06843 (SPT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein SPT2
Alternative name(s):
Negative regulator of Ty transcription
Gene names
Name:SPT2
Synonyms:SIN1, SPM2
Ordered Locus Names:YER161C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chromatin protein. Global regulatory protein that plays positive as well as negative regulatory roles in transcription.

Subunit structure

Interacts with SAP1 and CDC23. Component of the SAGA complex, a large multiprotein complex that modifies the chromatin, at least composed of SPT2, SPT7, SPT8, SPT20/ADA5, HFI1/ADA1, ADA2, ADA3/NGG1, TRA1 and GCN5. Ref.5

Subcellular location

Nucleus.

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SPT2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 333332Protein SPT2
PRO_0000072163

Regions

Coiled coil218 – 24225 Potential
Coiled coil292 – 31625 Potential

Amino acid modifications

Modified residue21N-acetylserine Ref.8
Modified residue1261Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
P06843 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 60C3BED7EEA99535

FASTA33338,551
        10         20         30         40         50         60 
MSFLSKLSQI RKSTTASKAQ VQDPLPKKND EEYSLLPKNY IRDEDPAVKR LKELRRQELL 

        70         80         90        100        110        120 
KNGALAKKSG VKRKRGTSSG SEKKKIERND DDEGGLGIRF KRSIGASHAP LKPVVRKKPE 

       130        140        150        160        170        180 
PIKKMSFEEL MKQAENNEKQ PPKVKSSEPV TKERPHFNKP GFKSSKRPQK KASPGATLRG 

       190        200        210        220        230        240 
VSSGGNSIKS SDSPKPVKLN LPTNGFAQPN RRLKEKLESR KQKSRYQDDY DEEDNDMDDF 

       250        260        270        280        290        300 
IEDDEDEGYH SKSKHSNGPG YDRDEIWAMF NRGKKRSEYD YDELEDDDME ANEMEILEEE 

       310        320        330 
EMARKMARLE DKREEAWLKK HEEEKRRRKK GIR 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the SPT2 gene, a negative regulator of Ty-controlled yeast gene expression."
Roeder G.S., Beard C., Smith M., Keranen S.
Mol. Cell. Biol. 5:1543-1553(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1."
Kruger W., Herskowitz I.
Mol. Cell. Biol. 11:4135-4146(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The ATM-related cofactor Tra1 is a component of the purified SAGA complex."
Grant P.A., Schieltz D., Pray-Grant M.G., Yates J.R. III, Workman J.L.
Mol. Cell 2:863-867(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A SAGA COMPLEX WITH SPT7; HFI1; SPT8; GCN5; SPT20; ADA2; ADA3 AND TRA1.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11165 Genomic DNA. Translation: AAA35083.1.
U18917 Genomic DNA. Translation: AAB64688.1.
BK006939 Genomic DNA. Translation: DAA07823.1.
PIRA23438.
RefSeqNP_011088.1. NM_001179051.1.

3D structure databases

ProteinModelPortalP06843.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36914. 184 interactions.
DIPDIP-1657N.
IntActP06843. 3 interactions.
MINTMINT-397095.
STRING4932.YER161C.

Proteomic databases

MaxQBP06843.
PaxDbP06843.
PeptideAtlasP06843.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER161C; YER161C; YER161C.
GeneID856908.
KEGGsce:YER161C.

Organism-specific databases

CYGDYER161c.
SGDS000000963. SPT2.

Phylogenomic databases

eggNOGNOG81249.
HOGENOMHOG000141754.
KOK15193.
OMAMEANEME.
OrthoDBEOG7J70S8.

Enzyme and pathway databases

BioCycYEAST:G3O-30322-MONOMER.

Gene expression databases

GenevestigatorP06843.

Family and domain databases

InterProIPR013256. Chromatin_SPT2.
[Graphical view]
PfamPF08243. SPT2. 1 hit.
[Graphical view]
SMARTSM00784. SPT2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983344.

Entry information

Entry nameSPT2_YEAST
AccessionPrimary (citable) accession number: P06843
Secondary accession number(s): D3DM69
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: May 14, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families