P06843 (SPT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SPT2

Alternative name(s):
Negative regulator of Ty transcription

Gene names

Name:	SPT2
Synonyms:	SIN1, SPM2
Ordered Locus Names:	YER161C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chromatin protein. Global regulatory protein that plays positive as well as negative regulatory roles in transcription.
Subunit structure	Interacts with SAP1 and CDC23. Component of the SAGA complex, a large multiprotein complex that modifies the chromatin, at least composed of SPT2, SPT7, SPT8, SPT20/ADA5, HFI1/ADA1, ADA2, ADA3/NGG1, TRA1 and GCN5. Ref.5
Subcellular location	Nucleus.
Miscellaneous	Present with 1240 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SPT2 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA polyadenylation Inferred from mutant phenotype PubMed 16788068. Source: SGD chromatin organization Inferred from mutant phenotype PubMed 16449659PubMed 19057669. Source: SGD negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 17526727. Source: SGD transcription elongation from RNA polymerase II promoter Inferred from genetic interaction PubMed 16449659. Source: SGD
Cellular_component	nucleus Inferred from direct assay Ref.2. Source: SGD
Molecular_function	DNA secondary structure binding Inferred from direct assay PubMed 15563464. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Protein SPT2		PRO_0000072163
Regions
	Coiled coil	218 – 242	25	Potential
	Coiled coil	292 – 316	25	Potential
Amino acid modifications
	Modified residue	40	1	Phosphotyrosine Ref.8
	Modified residue	126	1	Phosphoserine Ref.8
	Modified residue	173	1	Phosphoserine Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P06843 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 60C3BED7EEA99535	FASTA	333	38,551
	10 20 30 40 50 60 MSFLSKLSQI RKSTTASKAQ VQDPLPKKND EEYSLLPKNY IRDEDPAVKR LKELRRQELL 70 80 90 100 110 120 KNGALAKKSG VKRKRGTSSG SEKKKIERND DDEGGLGIRF KRSIGASHAP LKPVVRKKPE 130 140 150 160 170 180 PIKKMSFEEL MKQAENNEKQ PPKVKSSEPV TKERPHFNKP GFKSSKRPQK KASPGATLRG 190 200 210 220 230 240 VSSGGNSIKS SDSPKPVKLN LPTNGFAQPN RRLKEKLESR KQKSRYQDDY DEEDNDMDDF 250 260 270 280 290 300 IEDDEDEGYH SKSKHSNGPG YDRDEIWAMF NRGKKRSEYD YDELEDDDME ANEMEILEEE 310 320 330 EMARKMARLE DKREEAWLKK HEEEKRRRKK GIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the SPT2 gene, a negative regulator of Ty-controlled yeast gene expression." Roeder G.S., Beard C., Smith M., Keranen S. Mol. Cell. Biol. 5:1543-1553(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1." Kruger W., Herskowitz I. Mol. Cell. Biol. 11:4135-4146(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The ATM-related cofactor Tra1 is a component of the purified SAGA complex." Grant P.A., Schieltz D., Pray-Grant M.G., Yates J.R. III, Workman J.L. Mol. Cell 2:863-867(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A SAGA COMPLEX WITH SPT7; HFI1; SPT8; GCN5; SPT20; ADA2; ADA3 AND TRA1.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-40; SER-126 AND SER-173, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11165 Genomic DNA. Translation: AAA35083.1. U18917 Genomic DNA. Translation: AAB64688.1. BK006939 Genomic DNA. Translation: DAA07823.1.
PIR	A23438.
RefSeq	NP_011088.1. NM_001179051.1.
3D structure databases
ProteinModelPortal	P06843.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1657N.
IntAct	P06843. 25 interactions.
MINT	MINT-397095.
STRING	4932.YER161C.
Proteomic databases
PaxDb	P06843.
PeptideAtlas	P06843.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER161C; YER161C; YER161C.
GeneID	856908.
KEGG	sce:YER161C.
Organism-specific databases
CYGD	YER161c.
SGD	S000000963. SPT2.
Phylogenomic databases
eggNOG	NOG81249.
HOGENOM	HOG000141754.
KO	K15193.
OMA	MEANEME.
OrthoDB	EOG4936TN.
Gene expression databases
Genevestigator	P06843.
GermOnline	YER161C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013256. Chromatin_SPT2. [Graphical view]
Pfam	PF08243. SPT2. 1 hit. [Graphical view]
SMART	SM00784. SPT2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983344.

Entry information

Entry name

SPT2_YEAST

Accession

Primary (citable) accession number: P06843
Secondary accession number(s): D3DM69

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program