P06838 (RAD10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair protein RAD10

Gene names

Name:	RAD10
Ordered Locus Names:	YML095C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Along with RAD1 forms an endonuclease that specifically degrades single-stranded DNA. Ref.6
Subunit structure	Component of the nucleotide excision repair factor 1 (NEF1) complex consisting of RAD1, RAD10 and RAD14. Interacts with SAW1. Ref.6 Ref.8
Subcellular location	Nucleus.
Sequence similarities	Belongs to the ERCC1/RAD10/SWI10 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	double-strand break repair via single-strand annealing, removal of nonhomologous ends Inferred from mutant phenotype. Source: SGD meiotic mismatch repair Inferred from mutant phenotype. Source: SGD nucleotide-excision repair, DNA incision, 5'-to lesion Inferred from direct assay. Source: SGD
Cellular component	nucleus Inferred from direct assay. Source: SGD
Molecular function	DNA strand annealing activity Inferred from direct assay. Source: SGD damaged DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RAD1	P06777	8	EBI-14637,EBI-14752

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	DNA repair protein RAD10		PRO_0000097149
Regions
	DNA binding	133 – 153	21	Potential
	Motif	17 – 23	7	Nuclear localization signal Potential

Sequences

	Sequence		Length	Mass (Da)
	P06838 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 26891AD9CA1E3E2B	FASTA	210	24,311
	10 20 30 40 50 60 MNNTDPTSFE SILAGVAKLR KEKSGADTTG SQSLEIDASK LQQQEPQTSR RINSNQVINA 70 80 90 100 110 120 FNQQKPEEWT DSKATDDYNR KRPFRSTRPG KTVLVNTTQK ENPLLNHLKS TNWRYVSSTG 130 140 150 160 170 180 INMIYYDYLV RGRSVLFLTL TYHKLYVDYI SRRMQPLSRN ENNILIFIVD DNNSEDTLND 190 200 210 ITKLCMFNGF TLLLAFNFEQ AAKYIEYLNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli." Weiss W.A., Friedberg E.C. EMBO J. 4:1575-1582(1985) [PubMed: 3896774] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erratum Weiss W.A., Friedberg E.C. EMBO J. 4:3907-3907(1985) Cited for: SEQUENCE REVISION.
[3]	"Nucleotide sequence of the RAD10 gene of Saccharomyces cerevisiae." Reynolds P., Prakash L., Dumais D., Perozzi G., Prakash S. EMBO J. 4:3549-3552(1985) [PubMed: 3912171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single-stranded-DNA endonuclease." Tomkinson A.E., Bardwell A.J., Bardwell L., Tappe N.J., Friedberg E.C. Nature 362:860-862(1993) [PubMed: 8479526] [Abstract] Cited for: FUNCTION, INTERACTION WITH RAD1.
[7]	"Nucleotide excision repair in yeast is mediated by sequential assembly of repair factors and not by a pre-assembled repairosome." Guzder S.N., Sung P., Prakash L., Prakash S. J. Biol. Chem. 271:8903-8910(1996) [PubMed: 8621533] [Abstract] Cited for: IDENTIFICATION IN THE NEF1 COMPLEX.
[8]	"Examining protein protein interactions using endogenously tagged yeast arrays: the cross-and-capture system." Suter B., Fetchko M.J., Imhof R., Graham C.I., Stoffel-Studer I., Zbinden C., Raghavan M., Lopez L., Beneti L., Hort J., Fillingham J., Greenblatt J.F., Giaever G., Nislow C., Stagljar I. Genome Res. 17:1774-1782(2007) [PubMed: 17989249] [Abstract] Cited for: INTERACTION WITH SAW1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02591 Genomic DNA. Translation: CAA26433.1. X05225 Genomic DNA. Translation: CAA28856.1. Z46660 Genomic DNA. Translation: CAA86642.1. BK006946 Genomic DNA. Translation: DAA09804.1.
PIR	A24576.
RefSeq	NP_013614.1. NM_001182454.1.
3D structure databases
ProteinModelPortal	P06838.
SMR	P06838. Positions 91-209.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1545N.
IntAct	P06838. 17 interactions.
MINT	MINT-391436.
STRING	P06838.
Proteomic databases
PeptideAtlas	P06838.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML095C; YML095C; YML095C.
GeneID	854878.
KEGG	sce:YML095C.
NMPDR	fig|4932.3.peg.4651.
Organism-specific databases
CYGD	YML095c.
SGD	S000004560. RAD10.
Phylogenomic databases
eggNOG	fuNOG09709.
GeneTree	EFGT00050000006444.
HOGENOM	HBG203506.
OMA	ENPLLNH.
OrthoDB	EOG4DBXQ6.
Gene expression databases
ArrayExpress	P06838.
Genevestigator	P06838.
GermOnline	YML095C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004579. DNA_repair_Rad10. IPR011335. Restrct_endonuc_II-like. [Graphical view]
KO	K10849.
Pfam	PF03834. Rad10. 1 hit. [Graphical view]
ProDom	PD013585. DNA_repair_Rad10. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF52980. Restrict_endonuc_II-like_core. 1 hit.
TIGRFAMs	TIGR00597. Rad10. 1 hit.
ProtoNet	Search...
Other
NextBio	977822.

Entry information

Entry name

RAD10_YEAST

Accession

Primary (citable) accession number: P06838
Secondary accession number(s): D6W0J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	December 14, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program