Neuromodulin - Mus musculus (Mouse)

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P06837 (NEUM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Neuromodulin

Alternative name(s):
Axonal membrane protein GAP-43
Calmodulin-binding protein P-57
Growth-associated protein 43

Gene names

Name:	Gap43
Synonyms:	Basp2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Subunit structure	Binds calmodulin with a greater affinity in the absence of Ca²⁺ than in its presence By similarity. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection › growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction › synapse. Cell projection › filopodium membrane; Peripheral membrane protein By similarity. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.
Post-translational modification	Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction By similarity. Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Ref.5 Ref.6 Ref.7 Ref.8
Sequence similarities	Belongs to the neuromodulin family. Contains 1 IQ domain.

Ontologies

Keywords
Biological process	Differentiation Growth regulation Neurogenesis
Cellular component	Cell junction Cell membrane Cell projection Membrane Synapse
Ligand	Calmodulin-binding
Molecular function	Developmental protein
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	axon choice point recognition Inferred from mutant phenotype. Source: MGI cell fate commitment Inferred from mutant phenotype. Source: MGI glial cell differentiation Inferred from mutant phenotype. Source: MGI regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	axon Inferred from direct assay. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW filopodium membrane Inferred from electronic annotation. Source: UniProtKB-SubCell growth cone membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 227

227

Neuromodulin

PRO_0000159597

Regions

Domain

31 – 60

Region

1 – 4

Important for membrane binding

Amino acid modifications

Modified residue

Phosphoserine; by PHK Probable

Modified residue

Phosphothreonine Ref.8

Modified residue

Phosphoserine Ref.5

Modified residue

103

Phosphoserine Ref.8

Modified residue

172

Phosphothreonine Ref.5 Ref.7 Ref.8

Modified residue

192

Phosphoserine; by CK2 By similarity

Modified residue

193

Phosphoserine; by CK2 By similarity

Lipidation

S-palmitoyl cysteine By similarity

Lipidation

S-palmitoyl cysteine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P06837 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 14803B2F8F0649F7
Show »

FASTA

227

23,632

        10         20         30         40         50         60 
MLCCMRRTKQ VEKNDEDQKI EQDGVKPEDK AHKAATKIQA SFRGHITRKK LKGEKKGDAP 

        70         80         90        100        110        120 
AAEAEAKEKD DAPVADGVEK KEGDGSATTD AAPATSPKAE EPSKAGDAPS EEKKGEGDAA 

       130        140        150        160        170        180 
PSEEKAGSAE TESAAKATTD NSPSSKAEDG PAKEEPKQAD VPAAVTDAAA TTPAAEDAAT 

       190        200        210        220 
KAAQPPTETA ESSQAEEEKD AVDEAKPKES ARQDEGKEDP EADQEHA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of murine cDNAs encoding P-57, a neural-specific calmodulin-binding protein." Cimler B.M., Giebelhaus D.H., Wakim B.T., Storm D.R., Moon R.T. J. Biol. Chem. 262:12158-12163(1987) [PubMed: 2442159] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Retina.
[3]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 84-104, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[4]	"N-CAM modulates tumour-cell adhesion to matrix by inducing FGF-receptor signalling." Cavallaro U., Niedermeyer J., Fuxa M., Christofori G. Nat. Cell Biol. 3:650-657(2001) [PubMed: 11433297] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH NCAM1; CDH2; PLCG1; FRS2; SRC; FGFR4 AND CTTN.
[5]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96 AND THR-172, MASS SPECTROMETRY. Tissue: Embryonic brain.
[6]	"Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." Vosseller K., Hansen K.C., Chalkley R.J., Trinidad J.C., Wells L., Hart G.W., Burlingame A.L. Proteomics 5:388-398(2005) [PubMed: 15648052] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[7]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-172, MASS SPECTROMETRY. Tissue: Brain.
[8]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-95; SER-103 AND THR-172, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02809 mRNA. Translation: AAA37377.1. BC028288 mRNA. Translation: AAH28288.1. BC080758 mRNA. Translation: AAH80758.1.
IPI	IPI00128973.
PIR	A29800.
RefSeq	NP_032109.1. NM_008083.2.
UniGene	Mm.1222.
3D structure databases
ProteinModelPortal	P06837.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06837. 4 interactions.
STRING	P06837.
PTM databases
PhosphoSite	P06837.
Proteomic databases
PRIDE	P06837.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000102817; ENSMUSP00000099881; ENSMUSG00000047261.
GeneID	14432.
KEGG	mmu:14432.
UCSC	uc007zfv.1. mouse.
Organism-specific databases
CTD	2596.
MGI	MGI:95639. Gap43.
Phylogenomic databases
eggNOG	roNOG16581.
GeneTree	ENSGT00530000063966.
HOGENOM	HBG282505.
HOVERGEN	HBG006468.
InParanoid	P06837.
OMA	READQEH.
Gene expression databases
ArrayExpress	P06837.
Bgee	P06837.
CleanEx	MM_GAP43.
Genevestigator	P06837.
GermOnline	ENSMUSG00000047261. Mus musculus.
Family and domain databases
InterPro	IPR000048. IQ_motif_EF-hand-BS. IPR001422. Neuromodulin. IPR017454. Neuromodulin_C. IPR018947. Neuromodulin_gap-junction_N. IPR018243. Neuromodulin_palmitoyl/P_site. [Graphical view]
Pfam	PF00612. IQ. 1 hit. PF06614. Neuromodulin. 1 hit. PF10580. Neuromodulin_N. 1 hit. [Graphical view]
PRINTS	PR00215. NEUROMODULIN.
SMART	SM00015. IQ. 1 hit. [Graphical view]
PROSITE	PS50096. IQ. 1 hit. PS00412. NEUROMODULIN_1. 1 hit. PS00413. NEUROMODULIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	286053.
SOURCE	Search...

Entry information

Entry name

NEUM_MOUSE

Accession

Primary (citable) accession number: P06837

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	January 25, 2012
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents