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P06837

- NEUM_MOUSE

UniProt

P06837 - NEUM_MOUSE

Protein

Neuromodulin

Gene

Gap43

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

    GO - Biological processi

    1. axon choice point recognition Source: MGI
    2. axon guidance Source: MGI
    3. cell fate commitment Source: MGI
    4. glial cell differentiation Source: MGI
    5. regulation of filopodium assembly Source: Ensembl
    6. regulation of growth Source: UniProtKB-KW
    7. tissue regeneration Source: Ensembl

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Growth regulation, Neurogenesis

    Keywords - Ligandi

    Calmodulin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuromodulin
    Alternative name(s):
    Axonal membrane protein GAP-43
    Calmodulin-binding protein P-57
    Growth-associated protein 43
    Gene namesi
    Name:Gap43
    Synonyms:Basp2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:95639. Gap43.

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: MGI
    2. cell junction Source: UniProtKB-KW
    3. cell periphery Source: MGI
    4. cytoplasm Source: Ensembl
    5. filopodium membrane Source: UniProtKB-SubCell
    6. growth cone membrane Source: UniProtKB-SubCell
    7. plasma membrane Source: MGI
    8. synapse Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227NeuromodulinPRO_0000159597Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi3 – 31S-palmitoyl cysteineBy similarity
    Lipidationi4 – 41S-palmitoyl cysteineBy similarity
    Modified residuei41 – 411Phosphoserine; by PHKCurated
    Modified residuei172 – 1721PhosphothreonineBy similarity
    Modified residuei192 – 1921Phosphoserine; by CK2By similarity
    Modified residuei193 – 1931Phosphoserine; by CK2By similarity

    Post-translational modificationi

    Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2 By similarity.By similarity
    Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP06837.
    PaxDbiP06837.
    PRIDEiP06837.

    PTM databases

    PhosphoSiteiP06837.

    Expressioni

    Gene expression databases

    BgeeiP06837.
    CleanExiMM_GAP43.
    GenevestigatoriP06837.

    Interactioni

    Subunit structurei

    Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence By similarity. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi199828. 1 interaction.
    IntActiP06837. 5 interactions.
    MINTiMINT-4103374.
    STRINGi10090.ENSMUSP00000099881.

    Structurei

    Secondary structure

    1
    227
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi35 – 5117

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4E53X-ray2.69A/B34-57[»]
    ProteinModelPortaliP06837.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 6030IQPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 44Important for membrane binding

    Sequence similaritiesi

    Belongs to the neuromodulin family.Curated
    Contains 1 IQ domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG120480.
    GeneTreeiENSGT00730000111265.
    HOGENOMiHOG000013014.
    HOVERGENiHBG006468.
    InParanoidiP06837.
    OMAiVEKKGEG.
    OrthoDBiEOG744TBB.
    PhylomeDBiP06837.
    TreeFamiTF333213.

    Family and domain databases

    InterProiIPR000048. IQ_motif_EF-hand-BS.
    IPR001422. Neuromodulin.
    IPR017454. Neuromodulin_C.
    IPR018947. Neuromodulin_gap-junction_N.
    IPR018243. Neuromodulin_palmitoyl/P_site.
    [Graphical view]
    PfamiPF00612. IQ. 1 hit.
    PF06614. Neuromodulin. 1 hit.
    PF10580. Neuromodulin_N. 1 hit.
    [Graphical view]
    PRINTSiPR00215. NEUROMODULIN.
    SMARTiSM00015. IQ. 1 hit.
    [Graphical view]
    PROSITEiPS50096. IQ. 1 hit.
    PS00412. NEUROMODULIN_1. 1 hit.
    PS00413. NEUROMODULIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P06837-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLCCMRRTKQ VEKNDEDQKI EQDGVKPEDK AHKAATKIQA SFRGHITRKK    50
    LKGEKKGDAP AAEAEAKEKD DAPVADGVEK KEGDGSATTD AAPATSPKAE 100
    EPSKAGDAPS EEKKGEGDAA PSEEKAGSAE TESAAKATTD NSPSSKAEDG 150
    PAKEEPKQAD VPAAVTDAAA TTPAAEDAAT KAAQPPTETA ESSQAEEEKD 200
    AVDEAKPKES ARQDEGKEDP EADQEHA 227
    Length:227
    Mass (Da):23,632
    Last modified:January 1, 1988 - v1
    Checksum:i14803B2F8F0649F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02809 mRNA. Translation: AAA37377.1.
    BC028288 mRNA. Translation: AAH28288.1.
    BC080758 mRNA. Translation: AAH80758.1.
    CCDSiCCDS28177.1.
    PIRiA29800.
    RefSeqiNP_032109.1. NM_008083.2.
    UniGeneiMm.1222.

    Genome annotation databases

    EnsembliENSMUST00000102817; ENSMUSP00000099881; ENSMUSG00000047261.
    GeneIDi14432.
    KEGGimmu:14432.
    UCSCiuc007zfv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02809 mRNA. Translation: AAA37377.1 .
    BC028288 mRNA. Translation: AAH28288.1 .
    BC080758 mRNA. Translation: AAH80758.1 .
    CCDSi CCDS28177.1.
    PIRi A29800.
    RefSeqi NP_032109.1. NM_008083.2.
    UniGenei Mm.1222.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4E53 X-ray 2.69 A/B 34-57 [» ]
    ProteinModelPortali P06837.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199828. 1 interaction.
    IntActi P06837. 5 interactions.
    MINTi MINT-4103374.
    STRINGi 10090.ENSMUSP00000099881.

    PTM databases

    PhosphoSitei P06837.

    Proteomic databases

    MaxQBi P06837.
    PaxDbi P06837.
    PRIDEi P06837.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102817 ; ENSMUSP00000099881 ; ENSMUSG00000047261 .
    GeneIDi 14432.
    KEGGi mmu:14432.
    UCSCi uc007zfv.1. mouse.

    Organism-specific databases

    CTDi 2596.
    MGIi MGI:95639. Gap43.

    Phylogenomic databases

    eggNOGi NOG120480.
    GeneTreei ENSGT00730000111265.
    HOGENOMi HOG000013014.
    HOVERGENi HBG006468.
    InParanoidi P06837.
    OMAi VEKKGEG.
    OrthoDBi EOG744TBB.
    PhylomeDBi P06837.
    TreeFami TF333213.

    Miscellaneous databases

    NextBioi 286053.
    PROi P06837.
    SOURCEi Search...

    Gene expression databases

    Bgeei P06837.
    CleanExi MM_GAP43.
    Genevestigatori P06837.

    Family and domain databases

    InterProi IPR000048. IQ_motif_EF-hand-BS.
    IPR001422. Neuromodulin.
    IPR017454. Neuromodulin_C.
    IPR018947. Neuromodulin_gap-junction_N.
    IPR018243. Neuromodulin_palmitoyl/P_site.
    [Graphical view ]
    Pfami PF00612. IQ. 1 hit.
    PF06614. Neuromodulin. 1 hit.
    PF10580. Neuromodulin_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00215. NEUROMODULIN.
    SMARTi SM00015. IQ. 1 hit.
    [Graphical view ]
    PROSITEi PS50096. IQ. 1 hit.
    PS00412. NEUROMODULIN_1. 1 hit.
    PS00413. NEUROMODULIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of murine cDNAs encoding P-57, a neural-specific calmodulin-binding protein."
      Cimler B.M., Giebelhaus D.H., Wakim B.T., Storm D.R., Moon R.T.
      J. Biol. Chem. 262:12158-12163(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Retina.
    3. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 84-104, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    4. "N-CAM modulates tumour-cell adhesion to matrix by inducing FGF-receptor signalling."
      Cavallaro U., Niedermeyer J., Fuxa M., Christofori G.
      Nat. Cell Biol. 3:650-657(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH NCAM1; CDH2; PLCG1; FRS2; SRC; FGFR4 AND CTTN.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiNEUM_MOUSE
    AccessioniPrimary (citable) accession number: P06837
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3