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Protein

Neuromodulin

Gene

Gap43

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

GO - Molecular functioni

GO - Biological processi

  • axon choice point recognition Source: MGI
  • axon guidance Source: MGI
  • cell fate commitment Source: MGI
  • glial cell differentiation Source: MGI
  • regulation of filopodium assembly Source: MGI
  • regulation of growth Source: UniProtKB-KW
  • tissue regeneration Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Growth regulation, Neurogenesis

Keywords - Ligandi

Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuromodulin
Alternative name(s):
Axonal membrane protein GAP-43
Calmodulin-binding protein P-57
Growth-associated protein 43
Gene namesi
Name:Gap43
Synonyms:Basp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:95639. Gap43.

Subcellular locationi

GO - Cellular componenti

  • axon Source: MGI
  • cell junction Source: UniProtKB-KW
  • cell periphery Source: MGI
  • cytoplasm Source: MGI
  • filopodium membrane Source: MGI
  • growth cone membrane Source: UniProtKB-SubCell
  • neuronal postsynaptic density Source: MGI
  • plasma membrane Source: MGI
  • postsynaptic density Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227NeuromodulinPRO_0000159597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi3 – 31S-palmitoyl cysteineBy similarity
Lipidationi4 – 41S-palmitoyl cysteineBy similarity
Modified residuei41 – 411Phosphoserine; by PHKCurated
Modified residuei172 – 1721PhosphothreonineBy similarity
Modified residuei192 – 1921Phosphoserine; by CK2By similarity
Modified residuei193 – 1931Phosphoserine; by CK2By similarity

Post-translational modificationi

Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2 (By similarity).By similarity
Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP06837.
PaxDbiP06837.
PRIDEiP06837.

PTM databases

PhosphoSiteiP06837.

Expressioni

Gene expression databases

BgeeiP06837.
CleanExiMM_GAP43.
GenevisibleiP06837. MM.

Interactioni

Subunit structurei

Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence (By similarity). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN.By similarity1 Publication

Protein-protein interaction databases

BioGridi199828. 1 interaction.
IntActiP06837. 5 interactions.
MINTiMINT-4103374.
STRINGi10090.ENSMUSP00000099881.

Structurei

Secondary structure

1
227
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 5117Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4E53X-ray2.69A/B34-57[»]
ProteinModelPortaliP06837.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 6030IQPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 44Important for membrane binding

Sequence similaritiesi

Belongs to the neuromodulin family.Curated
Contains 1 IQ domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG120480.
GeneTreeiENSGT00730000111265.
HOGENOMiHOG000013014.
HOVERGENiHBG006468.
InParanoidiP06837.
OMAiPEADQEH.
OrthoDBiEOG744TBB.
PhylomeDBiP06837.
TreeFamiTF333213.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001422. Neuromodulin.
IPR017454. Neuromodulin_C.
IPR018947. Neuromodulin_gap-junction_N.
IPR018243. Neuromodulin_palmitoyl/P_site.
[Graphical view]
PANTHERiPTHR10699:SF15. PTHR10699:SF15. 1 hit.
PfamiPF00612. IQ. 1 hit.
PF06614. Neuromodulin. 1 hit.
PF10580. Neuromodulin_N. 1 hit.
[Graphical view]
PRINTSiPR00215. NEUROMODULIN.
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
PS00412. NEUROMODULIN_1. 1 hit.
PS00413. NEUROMODULIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06837-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCCMRRTKQ VEKNDEDQKI EQDGVKPEDK AHKAATKIQA SFRGHITRKK
60 70 80 90 100
LKGEKKGDAP AAEAEAKEKD DAPVADGVEK KEGDGSATTD AAPATSPKAE
110 120 130 140 150
EPSKAGDAPS EEKKGEGDAA PSEEKAGSAE TESAAKATTD NSPSSKAEDG
160 170 180 190 200
PAKEEPKQAD VPAAVTDAAA TTPAAEDAAT KAAQPPTETA ESSQAEEEKD
210 220
AVDEAKPKES ARQDEGKEDP EADQEHA
Length:227
Mass (Da):23,632
Last modified:January 1, 1988 - v1
Checksum:i14803B2F8F0649F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02809 mRNA. Translation: AAA37377.1.
BC028288 mRNA. Translation: AAH28288.1.
BC080758 mRNA. Translation: AAH80758.1.
CCDSiCCDS28177.1.
PIRiA29800.
RefSeqiNP_032109.1. NM_008083.2.
UniGeneiMm.1222.

Genome annotation databases

EnsembliENSMUST00000102817; ENSMUSP00000099881; ENSMUSG00000047261.
GeneIDi14432.
KEGGimmu:14432.
UCSCiuc007zfv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02809 mRNA. Translation: AAA37377.1.
BC028288 mRNA. Translation: AAH28288.1.
BC080758 mRNA. Translation: AAH80758.1.
CCDSiCCDS28177.1.
PIRiA29800.
RefSeqiNP_032109.1. NM_008083.2.
UniGeneiMm.1222.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4E53X-ray2.69A/B34-57[»]
ProteinModelPortaliP06837.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199828. 1 interaction.
IntActiP06837. 5 interactions.
MINTiMINT-4103374.
STRINGi10090.ENSMUSP00000099881.

PTM databases

PhosphoSiteiP06837.

Proteomic databases

MaxQBiP06837.
PaxDbiP06837.
PRIDEiP06837.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102817; ENSMUSP00000099881; ENSMUSG00000047261.
GeneIDi14432.
KEGGimmu:14432.
UCSCiuc007zfv.1. mouse.

Organism-specific databases

CTDi2596.
MGIiMGI:95639. Gap43.

Phylogenomic databases

eggNOGiNOG120480.
GeneTreeiENSGT00730000111265.
HOGENOMiHOG000013014.
HOVERGENiHBG006468.
InParanoidiP06837.
OMAiPEADQEH.
OrthoDBiEOG744TBB.
PhylomeDBiP06837.
TreeFamiTF333213.

Miscellaneous databases

ChiTaRSiGap43. mouse.
NextBioi286053.
PROiP06837.
SOURCEiSearch...

Gene expression databases

BgeeiP06837.
CleanExiMM_GAP43.
GenevisibleiP06837. MM.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001422. Neuromodulin.
IPR017454. Neuromodulin_C.
IPR018947. Neuromodulin_gap-junction_N.
IPR018243. Neuromodulin_palmitoyl/P_site.
[Graphical view]
PANTHERiPTHR10699:SF15. PTHR10699:SF15. 1 hit.
PfamiPF00612. IQ. 1 hit.
PF06614. Neuromodulin. 1 hit.
PF10580. Neuromodulin_N. 1 hit.
[Graphical view]
PRINTSiPR00215. NEUROMODULIN.
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
PS00412. NEUROMODULIN_1. 1 hit.
PS00413. NEUROMODULIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of murine cDNAs encoding P-57, a neural-specific calmodulin-binding protein."
    Cimler B.M., Giebelhaus D.H., Wakim B.T., Storm D.R., Moon R.T.
    J. Biol. Chem. 262:12158-12163(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  3. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 84-104, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  4. "N-CAM modulates tumour-cell adhesion to matrix by inducing FGF-receptor signalling."
    Cavallaro U., Niedermeyer J., Fuxa M., Christofori G.
    Nat. Cell Biol. 3:650-657(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH NCAM1; CDH2; PLCG1; FRS2; SRC; FGFR4 AND CTTN.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiNEUM_MOUSE
AccessioniPrimary (citable) accession number: P06837
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: July 22, 2015
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.