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P06837 (NEUM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuromodulin
Alternative name(s):
Axonal membrane protein GAP-43
Calmodulin-binding protein P-57
Growth-associated protein 43
Gene names
Name:Gap43
Synonyms:Basp2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

Subunit structure

Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence By similarity. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Ref.4

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projectiongrowth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junctionsynapse. Cell projectionfilopodium membrane; Peripheral membrane protein By similarity. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Post-translational modification

Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2 By similarity.

Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.

Sequence similarities

Belongs to the neuromodulin family.

Contains 1 IQ domain.

Ontologies

Keywords
   Biological processDifferentiation
Growth regulation
Neurogenesis
   Cellular componentCell junction
Cell membrane
Cell projection
Membrane
Synapse
   LigandCalmodulin-binding
   Molecular functionDevelopmental protein
   PTMLipoprotein
Palmitate
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processaxon choice point recognition

Inferred from mutant phenotype PubMed 7859286. Source: MGI

axon guidance

Inferred from mutant phenotype PubMed 10662636. Source: MGI

cell fate commitment

Inferred from mutant phenotype PubMed 15234344. Source: MGI

glial cell differentiation

Inferred from mutant phenotype PubMed 15234344. Source: MGI

regulation of filopodium assembly

Inferred from electronic annotation. Source: Ensembl

regulation of growth

Inferred from electronic annotation. Source: UniProtKB-KW

tissue regeneration

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentaxon

Inferred from direct assay PubMed 10482234PubMed 10850491PubMed 15071120. Source: MGI

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cell periphery

Inferred from direct assay PubMed 21752992. Source: MGI

cytoplasm

Inferred from electronic annotation. Source: Ensembl

filopodium membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

growth cone membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from direct assay PubMed 16892058. Source: MGI

synapse

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Neuromodulin
PRO_0000159597

Regions

Domain31 – 6030IQ
Region1 – 44Important for membrane binding

Amino acid modifications

Modified residue411Phosphoserine; by PHK Probable
Modified residue1721Phosphothreonine By similarity
Modified residue1921Phosphoserine; by CK2 By similarity
Modified residue1931Phosphoserine; by CK2 By similarity
Lipidation31S-palmitoyl cysteine By similarity
Lipidation41S-palmitoyl cysteine By similarity

Secondary structure

... 227
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06837 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 14803B2F8F0649F7

FASTA22723,632
        10         20         30         40         50         60 
MLCCMRRTKQ VEKNDEDQKI EQDGVKPEDK AHKAATKIQA SFRGHITRKK LKGEKKGDAP 

        70         80         90        100        110        120 
AAEAEAKEKD DAPVADGVEK KEGDGSATTD AAPATSPKAE EPSKAGDAPS EEKKGEGDAA 

       130        140        150        160        170        180 
PSEEKAGSAE TESAAKATTD NSPSSKAEDG PAKEEPKQAD VPAAVTDAAA TTPAAEDAAT 

       190        200        210        220 
KAAQPPTETA ESSQAEEEKD AVDEAKPKES ARQDEGKEDP EADQEHA 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of murine cDNAs encoding P-57, a neural-specific calmodulin-binding protein."
Cimler B.M., Giebelhaus D.H., Wakim B.T., Storm D.R., Moon R.T.
J. Biol. Chem. 262:12158-12163(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Retina.
[3]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 84-104, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[4]"N-CAM modulates tumour-cell adhesion to matrix by inducing FGF-receptor signalling."
Cavallaro U., Niedermeyer J., Fuxa M., Christofori G.
Nat. Cell Biol. 3:650-657(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH NCAM1; CDH2; PLCG1; FRS2; SRC; FGFR4 AND CTTN.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[6]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02809 mRNA. Translation: AAA37377.1.
BC028288 mRNA. Translation: AAH28288.1.
BC080758 mRNA. Translation: AAH80758.1.
CCDSCCDS28177.1.
PIRA29800.
RefSeqNP_032109.1. NM_008083.2.
UniGeneMm.1222.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4E53X-ray2.69A/B34-57[»]
ProteinModelPortalP06837.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199828. 1 interaction.
IntActP06837. 5 interactions.
MINTMINT-4103374.
STRING10090.ENSMUSP00000099881.

PTM databases

PhosphoSiteP06837.

Proteomic databases

MaxQBP06837.
PaxDbP06837.
PRIDEP06837.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102817; ENSMUSP00000099881; ENSMUSG00000047261.
GeneID14432.
KEGGmmu:14432.
UCSCuc007zfv.1. mouse.

Organism-specific databases

CTD2596.
MGIMGI:95639. Gap43.

Phylogenomic databases

eggNOGNOG120480.
GeneTreeENSGT00730000111265.
HOGENOMHOG000013014.
HOVERGENHBG006468.
InParanoidP06837.
OMAVEKKGEG.
OrthoDBEOG744TBB.
PhylomeDBP06837.
TreeFamTF333213.

Gene expression databases

BgeeP06837.
CleanExMM_GAP43.
GenevestigatorP06837.

Family and domain databases

InterProIPR000048. IQ_motif_EF-hand-BS.
IPR001422. Neuromodulin.
IPR017454. Neuromodulin_C.
IPR018947. Neuromodulin_gap-junction_N.
IPR018243. Neuromodulin_palmitoyl/P_site.
[Graphical view]
PfamPF00612. IQ. 1 hit.
PF06614. Neuromodulin. 1 hit.
PF10580. Neuromodulin_N. 1 hit.
[Graphical view]
PRINTSPR00215. NEUROMODULIN.
SMARTSM00015. IQ. 1 hit.
[Graphical view]
PROSITEPS50096. IQ. 1 hit.
PS00412. NEUROMODULIN_1. 1 hit.
PS00413. NEUROMODULIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio286053.
PROP06837.
SOURCESearch...

Entry information

Entry nameNEUM_MOUSE
AccessionPrimary (citable) accession number: P06837
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot