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P06836 (NEUM_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuromodulin
Alternative name(s):
Axonal membrane protein GAP-43
Calmodulin-binding protein P-57
Growth-associated protein 43
Gene names
Name:GAP43
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction By similarity.

Subunit structure

Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN By similarity. Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projectiongrowth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junctionsynapse. Cell projectionfilopodium membrane; Peripheral membrane protein By similarity. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Post-translational modification

Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2 By similarity. Ref.7

Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Ref.4 Ref.5 Ref.8

Sequence similarities

Belongs to the neuromodulin family.

Contains 1 IQ domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 242242Neuromodulin
PRO_0000159595

Regions

Domain31 – 6030IQ
Region1 – 44Important for membrane binding

Amino acid modifications

Modified residue411Phosphoserine; by PHK and PKC Ref.4 Ref.8
Modified residue2061Phosphoserine; by CK2 Ref.5
Modified residue2071Phosphoserine; by CK2 Ref.5
Lipidation31S-palmitoyl cysteine Ref.7
Lipidation41S-palmitoyl cysteine Ref.7

Experimental info

Mutagenesis31C → G: Lack of palmitoylation; when associated with G-4. Ref.7
Mutagenesis31C → L: Decreased palmitoylation level. Ref.7
Mutagenesis41C → G: Lack of palmitoylation; when associated with G-3. Ref.7
Mutagenesis41C → L: Decreased palmitoylation level. Ref.7
Sequence conflict221Q → R in AAI18453. Ref.3
Sequence conflict111 – 1133Missing AA sequence Ref.1
Sequence conflict1341P → L in AAO60065. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P06836 [UniParc].

Last modified May 1, 2007. Version 3.
Checksum: FD1F9A014FA91796

FASTA24225,066
        10         20         30         40         50         60 
MLCCMRRTKQ VEKNDEDQKI EQDGIKPEDK AHKAATKIQA SFRGHITRKK LKGEKKGDAP 

        70         80         90        100        110        120 
AAEAEANEKD EAAVAEGTEK KEGEGSTPAE AAPGAGPKPE EKTGKAGETP SEEKKGEGAP 

       130        140        150        160        170        180 
DAATEQAAPQ APAPSEEKAG SAETESATKA STDNSPSSKA EDAPAKEEPK QADVPAAVTA 

       190        200        210        220        230        240 
AAATAPAAED AAAMATAQPP TETAESSQAE EKIEAVDETK PKDSARQDEG KGEEREADQE 


HA 

« Hide

References

« Hide 'large scale' references
[1]"Amino acid sequence of P-57, a neurospecific calmodulin-binding protein."
Wakim B.T., Alexander K.A., Masure H.R., Cimler B.M., Storm D.R., Walsh K.A.
Biochemistry 26:7466-7470(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[2]"Bovine GAP43 cDNA."
Haegeman A.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal cerebellum.
[4]"Identification of the protein kinase C phosphorylation site in neuromodulin."
Apel E.D., Byford M.F., Au D., Walsh K.A., Storm D.R.
Biochemistry 29:2330-2335(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-41 BY PKC.
[5]"Phosphorylation of neuromodulin (GAP-43) by casein kinase II. Identification of phosphorylation sites and regulation by calmodulin."
Apel E.D., Litchfield D.W., Cllark R.H., Krebs E.G., Storm D.R.
J. Biol. Chem. 266:10544-10551(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-206 AND SER-207 BY CKII.
[6]"Characterization of the calmodulin binding domain of neuromodulin. Functional significance of serine 41 and phenylalanine 42."
Chapman E.R., Au D., Alexander K.A., Nicolson T.A., Storm D.R.
J. Biol. Chem. 266:207-213(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: CALMODULIN-BINDING DOMAIN.
[7]"Analysis of the palmitoylation and membrane targeting domain of neuromodulin (GAP-43) by site-specific mutagenesis."
Liu Y., Fisher D.A., Storm D.R.
Biochemistry 32:10714-10719(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION AT CYS-3 AND CYS-4, MUTAGENESIS OF CYS-3 AND CYS-4.
[8]"Phosphorylase kinase phosphorylates the calmodulin-binding regulatory regions of neuronal tissue-specific proteins B-50 (GAP-43) and neurogranin."
Paudel H.K., Zwiers H., Wang J.H.
J. Biol. Chem. 268:6207-6213(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-41 BY PHK.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY195838 mRNA. Translation: AAO60065.1.
BC118452 mRNA. Translation: AAI18453.1.
PIRA27225.
RefSeqNP_976234.2. NM_203358.2.
UniGeneBt.5457.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000008460; ENSBTAP00000008460; ENSBTAG00000006451.
GeneID281777.
KEGGbta:281777.

Organism-specific databases

CTD2596.

Phylogenomic databases

eggNOGNOG120480.
GeneTreeENSGT00730000111265.
HOGENOMHOG000013014.
HOVERGENHBG006468.
InParanoidP06836.
OMAVEKKGEG.
OrthoDBEOG744TBB.
TreeFamTF333213.

Family and domain databases

InterProIPR000048. IQ_motif_EF-hand-BS.
IPR001422. Neuromodulin.
IPR017454. Neuromodulin_C.
IPR018947. Neuromodulin_gap-junction_N.
IPR018243. Neuromodulin_palmitoyl/P_site.
[Graphical view]
PfamPF00612. IQ. 1 hit.
PF06614. Neuromodulin. 1 hit.
PF10580. Neuromodulin_N. 1 hit.
[Graphical view]
PRINTSPR00215. NEUROMODULIN.
SMARTSM00015. IQ. 1 hit.
[Graphical view]
PROSITEPS50096. IQ. 1 hit.
PS00412. NEUROMODULIN_1. 1 hit.
PS00413. NEUROMODULIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805690.

Entry information

Entry nameNEUM_BOVIN
AccessionPrimary (citable) accession number: P06836
Secondary accession number(s): Q17QB5, Q6XZP9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: May 1, 2007
Last modified: February 19, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families