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P06832

- NPRE_BACAM

UniProt

P06832 - NPRE_BACAM

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Protein

Bacillolysin

Gene
npr
Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit Reviewed prediction.
Binds 1 zinc ion per subunit Reviewed prediction.

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi283 – 2831Calcium 1; via carbonyl oxygen Reviewed prediction
Metal bindingi360 – 3601Calcium 2 Reviewed prediction
Metal bindingi364 – 3641Zinc; catalytic By similarity
Active sitei365 – 3651 By similarity
Metal bindingi368 – 3681Zinc; catalytic By similarity
Metal bindingi388 – 3881Zinc; catalytic By similarity
Metal bindingi399 – 3991Calcium 2 Reviewed prediction
Metal bindingi399 – 3991Calcium 3 Reviewed prediction
Metal bindingi402 – 4021Calcium 2 Reviewed prediction
Metal bindingi402 – 4021Calcium 3 Reviewed prediction
Metal bindingi404 – 4041Calcium 2; via carbonyl oxygen Reviewed prediction
Metal bindingi407 – 4071Calcium 2 Reviewed prediction
Metal bindingi407 – 4071Calcium 3 Reviewed prediction
Metal bindingi411 – 4111Calcium 4; via carbonyl oxygen Reviewed prediction
Active sitei449 – 4491Proton donor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:npr
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Propeptidei28 – 221194Activation peptidePRO_0000028594Add
BLAST
Chaini222 – 521300BacillolysinPRO_0000028595Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliP06832.
SMRiP06832. Positions 222-521.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06832-1 [UniParc]FASTAAdd to Basket

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MGLGKKLSVA VAASFMSLTI SLPGVQAAEN PQLKENLTNF VPKHSLVQSE    50
LPSVSDKAIK QYLKQNGKVF KGNPSERLKL IDQTTDDLGY KHFRYVPVVN 100
GVPVKDSQVI IHVDKSNNVY AINGELNNDV SAKTANSKKL SANQALDHAY 150
KAIGKSPEAV SNGTVANKNK AELKAAATKD GKYRLAYDVT IRYIEPEPAN 200
WEVTVDAETG KILKKQNKVE HAATTGTGTT LKGKTVSLNI SSESGKYVLR 250
DLSKPTGTQI ITYDLQNREY NLPGTLVSST TNQFTTSSQR AAVDAHYNLG 300
KVYDYFYQKF NRNSYDNKGG KIVSSVHYGS RYNNAAWIGD QMIYGDGDGS 350
FFSPLSGSMD VTAHEMTHGV TQETANLNYE NQPGALNESF SDVFGYFNDT 400
EDWDIGEDIT VSQPALRSLS NPTKYGQPDN FKNYKNLPNT DAGDYGGVHT 450
NSGIPNKAAY NTITKIGVNK AEQIYYRALT VYLTPSSTFK DAKAALIQSA 500
RDLYGSQDAA SVEAAWNAVG L 521
Length:521
Mass (Da):56,840
Last modified:January 1, 1988 - v1
Checksum:i9609FA0416222751
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02497 Genomic DNA. Translation: AAB05346.1.
PIRiA25415. HYBSN.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02497 Genomic DNA. Translation: AAB05346.1 .
PIRi A25415. HYBSN.

3D structure databases

ProteinModelPortali P06832.
SMRi P06832. Positions 222-521.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M04.014.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein."
    Vasantha N., Thompson L.D., Rhodes C., Banner C., Nagle J., Filpula D.
    J. Bacteriol. 159:811-819(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 23844 / P.

Entry informationi

Entry nameiNPRE_BACAM
AccessioniPrimary (citable) accession number: P06832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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