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Protein

Bacillolysin

Gene

npr

Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+CuratedNote: Binds 4 Ca2+ ions per subunit.Curated
  • Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi283 – 2831Calcium 1; via carbonyl oxygenSequence Analysis
Metal bindingi360 – 3601Calcium 2Sequence Analysis
Metal bindingi364 – 3641Zinc; catalyticPROSITE-ProRule annotation
Active sitei365 – 3651PROSITE-ProRule annotation
Metal bindingi368 – 3681Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi388 – 3881Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi399 – 3991Calcium 2Sequence Analysis
Metal bindingi399 – 3991Calcium 3Sequence Analysis
Metal bindingi402 – 4021Calcium 2Sequence Analysis
Metal bindingi402 – 4021Calcium 3Sequence Analysis
Metal bindingi404 – 4041Calcium 2; via carbonyl oxygenSequence Analysis
Metal bindingi407 – 4071Calcium 2Sequence Analysis
Metal bindingi407 – 4071Calcium 3Sequence Analysis
Metal bindingi411 – 4111Calcium 4; via carbonyl oxygenSequence Analysis
Active sitei449 – 4491Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:npr
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 221194Activation peptidePRO_0000028594Add
BLAST
Chaini222 – 521300BacillolysinPRO_0000028595Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliP06832.
SMRiP06832. Positions 222-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLGKKLSVA VAASFMSLTI SLPGVQAAEN PQLKENLTNF VPKHSLVQSE
60 70 80 90 100
LPSVSDKAIK QYLKQNGKVF KGNPSERLKL IDQTTDDLGY KHFRYVPVVN
110 120 130 140 150
GVPVKDSQVI IHVDKSNNVY AINGELNNDV SAKTANSKKL SANQALDHAY
160 170 180 190 200
KAIGKSPEAV SNGTVANKNK AELKAAATKD GKYRLAYDVT IRYIEPEPAN
210 220 230 240 250
WEVTVDAETG KILKKQNKVE HAATTGTGTT LKGKTVSLNI SSESGKYVLR
260 270 280 290 300
DLSKPTGTQI ITYDLQNREY NLPGTLVSST TNQFTTSSQR AAVDAHYNLG
310 320 330 340 350
KVYDYFYQKF NRNSYDNKGG KIVSSVHYGS RYNNAAWIGD QMIYGDGDGS
360 370 380 390 400
FFSPLSGSMD VTAHEMTHGV TQETANLNYE NQPGALNESF SDVFGYFNDT
410 420 430 440 450
EDWDIGEDIT VSQPALRSLS NPTKYGQPDN FKNYKNLPNT DAGDYGGVHT
460 470 480 490 500
NSGIPNKAAY NTITKIGVNK AEQIYYRALT VYLTPSSTFK DAKAALIQSA
510 520
RDLYGSQDAA SVEAAWNAVG L
Length:521
Mass (Da):56,840
Last modified:December 31, 1987 - v1
Checksum:i9609FA0416222751
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02497 Genomic DNA. Translation: AAB05346.1.
PIRiA25415. HYBSN.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02497 Genomic DNA. Translation: AAB05346.1.
PIRiA25415. HYBSN.

3D structure databases

ProteinModelPortaliP06832.
SMRiP06832. Positions 222-521.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein."
    Vasantha N., Thompson L.D., Rhodes C., Banner C., Nagle J., Filpula D.
    J. Bacteriol. 159:811-819(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 23844 / P.

Entry informationi

Entry nameiNPRE_BACAM
AccessioniPrimary (citable) accession number: P06832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 31, 1987
Last sequence update: December 31, 1987
Last modified: January 6, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.