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Protein

Bacillolysin

Gene

npr

Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+CuratedNote: Binds 4 Ca2+ ions per subunit.Curated
  • Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi283Calcium 1; via carbonyl oxygenSequence analysis1
Metal bindingi360Calcium 2Sequence analysis1
Metal bindingi364Zinc; catalyticPROSITE-ProRule annotation1
Active sitei365PROSITE-ProRule annotation1
Metal bindingi368Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi388Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi399Calcium 2Sequence analysis1
Metal bindingi399Calcium 3Sequence analysis1
Metal bindingi402Calcium 2Sequence analysis1
Metal bindingi402Calcium 3Sequence analysis1
Metal bindingi404Calcium 2; via carbonyl oxygenSequence analysis1
Metal bindingi407Calcium 2Sequence analysis1
Metal bindingi407Calcium 3Sequence analysis1
Metal bindingi411Calcium 4; via carbonyl oxygenSequence analysis1
Active sitei449Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:npr
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000002859428 – 221Activation peptideAdd BLAST194
ChainiPRO_0000028595222 – 521BacillolysinAdd BLAST300

Keywords - PTMi

Zymogen

Interactioni

Protein-protein interaction databases

STRINGi326423.RBAM_014550.

Structurei

3D structure databases

ProteinModelPortaliP06832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105D4Y. Bacteria.
COG3227. LUCA.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06832-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLGKKLSVA VAASFMSLTI SLPGVQAAEN PQLKENLTNF VPKHSLVQSE
60 70 80 90 100
LPSVSDKAIK QYLKQNGKVF KGNPSERLKL IDQTTDDLGY KHFRYVPVVN
110 120 130 140 150
GVPVKDSQVI IHVDKSNNVY AINGELNNDV SAKTANSKKL SANQALDHAY
160 170 180 190 200
KAIGKSPEAV SNGTVANKNK AELKAAATKD GKYRLAYDVT IRYIEPEPAN
210 220 230 240 250
WEVTVDAETG KILKKQNKVE HAATTGTGTT LKGKTVSLNI SSESGKYVLR
260 270 280 290 300
DLSKPTGTQI ITYDLQNREY NLPGTLVSST TNQFTTSSQR AAVDAHYNLG
310 320 330 340 350
KVYDYFYQKF NRNSYDNKGG KIVSSVHYGS RYNNAAWIGD QMIYGDGDGS
360 370 380 390 400
FFSPLSGSMD VTAHEMTHGV TQETANLNYE NQPGALNESF SDVFGYFNDT
410 420 430 440 450
EDWDIGEDIT VSQPALRSLS NPTKYGQPDN FKNYKNLPNT DAGDYGGVHT
460 470 480 490 500
NSGIPNKAAY NTITKIGVNK AEQIYYRALT VYLTPSSTFK DAKAALIQSA
510 520
RDLYGSQDAA SVEAAWNAVG L
Length:521
Mass (Da):56,840
Last modified:January 1, 1988 - v1
Checksum:i9609FA0416222751
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02497 Genomic DNA. Translation: AAB05346.1.
PIRiA25415. HYBSN.
RefSeqiWP_014470445.1. NZ_LYUG01000019.1.

Genome annotation databases

GeneIDi9781016.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02497 Genomic DNA. Translation: AAB05346.1.
PIRiA25415. HYBSN.
RefSeqiWP_014470445.1. NZ_LYUG01000019.1.

3D structure databases

ProteinModelPortaliP06832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326423.RBAM_014550.

Protein family/group databases

MEROPSiM04.014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9781016.

Phylogenomic databases

eggNOGiENOG4105D4Y. Bacteria.
COG3227. LUCA.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPRE_BACAM
AccessioniPrimary (citable) accession number: P06832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.