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Reviewed, UniProtKB/Swiss-Prot P06832 (NPRE_BACAM)

Last modified June 16, 2009. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Bacillolysin
    EC=3.4.24.28
Alternative name(s):
    Neutral protease
Gene names
Name: npr
OrganismBacillus amyloliquefaciens
Taxonomic identifier1390 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Extracellular zinc metalloprotease.

Catalytic activity

Similar, but not identical, to that of thermolysin.

Cofactor

Binds 4 calcium ions per subunit Potential.

Binds 1 zinc ion per subunit Potential.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M4 family.

biophysicochemical properties

Temperature dependence:

Thermolabile.

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Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMZymogen
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Propeptide28 – 221194Activation peptide
PRO_0000028594
Chain222 – 521300Bacillolysin
PRO_0000028595

Sites

Active site3651 By similarity
Active site4491Proton donor By similarity
Metal binding2831Calcium 1; via carbonyl oxygen Potential
Metal binding3601Calcium 2 Potential
Metal binding3641Zinc; catalytic By similarity
Metal binding3681Zinc; catalytic By similarity
Metal binding3881Zinc; catalytic By similarity
Metal binding3991Calcium 2 Potential
Metal binding3991Calcium 3 Potential
Metal binding4021Calcium 2 Potential
Metal binding4021Calcium 3 Potential
Metal binding4041Calcium 2; via carbonyl oxygen Potential
Metal binding4071Calcium 2 Potential
Metal binding4071Calcium 3 Potential
Metal binding4111Calcium 4; via carbonyl oxygen Potential

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Sequences

Sequence LengthMass (Da)Tools
P06832-1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 9609FA0416222751

FASTA52156,840
        10         20         30         40         50         60 
MGLGKKLSVA VAASFMSLTI SLPGVQAAEN PQLKENLTNF VPKHSLVQSE LPSVSDKAIK 

        70         80         90        100        110        120 
QYLKQNGKVF KGNPSERLKL IDQTTDDLGY KHFRYVPVVN GVPVKDSQVI IHVDKSNNVY 

       130        140        150        160        170        180 
AINGELNNDV SAKTANSKKL SANQALDHAY KAIGKSPEAV SNGTVANKNK AELKAAATKD 

       190        200        210        220        230        240 
GKYRLAYDVT IRYIEPEPAN WEVTVDAETG KILKKQNKVE HAATTGTGTT LKGKTVSLNI 

       250        260        270        280        290        300 
SSESGKYVLR DLSKPTGTQI ITYDLQNREY NLPGTLVSST TNQFTTSSQR AAVDAHYNLG 

       310        320        330        340        350        360 
KVYDYFYQKF NRNSYDNKGG KIVSSVHYGS RYNNAAWIGD QMIYGDGDGS FFSPLSGSMD 

       370        380        390        400        410        420 
VTAHEMTHGV TQETANLNYE NQPGALNESF SDVFGYFNDT EDWDIGEDIT VSQPALRSLS 

       430        440        450        460        470        480 
NPTKYGQPDN FKNYKNLPNT DAGDYGGVHT NSGIPNKAAY NTITKIGVNK AEQIYYRALT 

       490        500        510        520 
VYLTPSSTFK DAKAALIQSA RDLYGSQDAA SVEAAWNAVG L

« Hide

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References

[1]"Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein."
Vasantha N., Thompson L.D., Rhodes C., Banner C., Nagle J., Filpula D.
J. Bacteriol. 159:811-819(1984) [PubMed: 6090391] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23844 / P.

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Cross-references

Sequence databases

K02497 Genomic DNA. Translation: AAB05346.1.
PIRHYBSN. A25415.

3D structure databases

HSSPHSSP built from PDB template 1BQB based on UniProtKB P81177.
ModBaseSearch...

Protein family/group databases

MEROPSM04.014.

Enzyme and pathway databases

BRENDA3.4.24.28. 1130.

Family and domain databases

InterProIPR005075. Pept_M4_propep_PepSY.
IPR006025. Pept_M_Zn_BS.
IPR013856. Peptidase_M4.
IPR001570. Peptidase_M4_C.
IPR011096. Propep_M4_M36.
[Graphical view]
Gene3DG3DSA:3.10.170.10. Peptidase_M4. 1 hit.
G3DSA:1.10.390.10. Peptidase_M4/M36. 1 hit.
PfamPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSPR00730. THERMOLYSIN.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNPRE_BACAM
AccessionPrimary (citable) accession number: P06832
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents