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P06832

- NPRE_BACAM

UniProt

P06832 - NPRE_BACAM

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Protein
Bacillolysin
Gene
npr
Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit Reviewed prediction.
Binds 1 zinc ion per subunit Reviewed prediction.

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi283 – 2831Calcium 1; via carbonyl oxygen Reviewed prediction
Metal bindingi360 – 3601Calcium 2 Reviewed prediction
Metal bindingi364 – 3641Zinc; catalytic By similarity
Active sitei365 – 3651 By similarity
Metal bindingi368 – 3681Zinc; catalytic By similarity
Metal bindingi388 – 3881Zinc; catalytic By similarity
Metal bindingi399 – 3991Calcium 2 Reviewed prediction
Metal bindingi399 – 3991Calcium 3 Reviewed prediction
Metal bindingi402 – 4021Calcium 2 Reviewed prediction
Metal bindingi402 – 4021Calcium 3 Reviewed prediction
Metal bindingi404 – 4041Calcium 2; via carbonyl oxygen Reviewed prediction
Metal bindingi407 – 4071Calcium 2 Reviewed prediction
Metal bindingi407 – 4071Calcium 3 Reviewed prediction
Metal bindingi411 – 4111Calcium 4; via carbonyl oxygen Reviewed prediction
Active sitei449 – 4491Proton donor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM04.014.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bacillolysin (EC:3.4.24.28)
    Alternative name(s):
    Neutral protease
    Gene namesi
    Name:npr
    OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
    Taxonomic identifieri1390 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727 Reviewed prediction
    Add
    BLAST
    Propeptidei28 – 221194Activation peptide
    PRO_0000028594Add
    BLAST
    Chaini222 – 521300Bacillolysin
    PRO_0000028595Add
    BLAST

    Keywords - PTMi

    Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliP06832.
    SMRiP06832. Positions 222-521.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M4 family.

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.10.170.10. 1 hit.
    InterProiIPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view]
    PfamiPF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view]
    PRINTSiPR00730. THERMOLYSIN.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06832-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLGKKLSVA VAASFMSLTI SLPGVQAAEN PQLKENLTNF VPKHSLVQSE    50
    LPSVSDKAIK QYLKQNGKVF KGNPSERLKL IDQTTDDLGY KHFRYVPVVN 100
    GVPVKDSQVI IHVDKSNNVY AINGELNNDV SAKTANSKKL SANQALDHAY 150
    KAIGKSPEAV SNGTVANKNK AELKAAATKD GKYRLAYDVT IRYIEPEPAN 200
    WEVTVDAETG KILKKQNKVE HAATTGTGTT LKGKTVSLNI SSESGKYVLR 250
    DLSKPTGTQI ITYDLQNREY NLPGTLVSST TNQFTTSSQR AAVDAHYNLG 300
    KVYDYFYQKF NRNSYDNKGG KIVSSVHYGS RYNNAAWIGD QMIYGDGDGS 350
    FFSPLSGSMD VTAHEMTHGV TQETANLNYE NQPGALNESF SDVFGYFNDT 400
    EDWDIGEDIT VSQPALRSLS NPTKYGQPDN FKNYKNLPNT DAGDYGGVHT 450
    NSGIPNKAAY NTITKIGVNK AEQIYYRALT VYLTPSSTFK DAKAALIQSA 500
    RDLYGSQDAA SVEAAWNAVG L 521
    Length:521
    Mass (Da):56,840
    Last modified:January 1, 1988 - v1
    Checksum:i9609FA0416222751
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02497 Genomic DNA. Translation: AAB05346.1.
    PIRiA25415. HYBSN.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02497 Genomic DNA. Translation: AAB05346.1 .
    PIRi A25415. HYBSN.

    3D structure databases

    ProteinModelPortali P06832.
    SMRi P06832. Positions 222-521.
    ModBasei Search...

    Protein family/group databases

    MEROPSi M04.014.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.10.170.10. 1 hit.
    InterProi IPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view ]
    Pfami PF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00730. THERMOLYSIN.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein."
      Vasantha N., Thompson L.D., Rhodes C., Banner C., Nagle J., Filpula D.
      J. Bacteriol. 159:811-819(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 23844 / P.

    Entry informationi

    Entry nameiNPRE_BACAM
    AccessioniPrimary (citable) accession number: P06832
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: May 14, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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