Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calpain-1 catalytic subunit

Gene

CAPN1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.By similarity

Catalytic activityi

Broad endopeptidase specificity.By similarity

Cofactori

Ca2+By similarity1 PublicationNote: Binds 4 Ca2+ ions.By similarity1 Publication

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi186 – 19711 PublicationAdd BLAST12
Calcium bindingi216 – 22721 PublicationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.52. 1749.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunitCurated (EC:3.4.22.52By similarity)
Alternative name(s):
Calcium-activated neutral proteinase 1By similarity
Short name:
CANP 1By similarity
Calpain mu-typeBy similarity
Calpain-1 large subunitBy similarity
Micromolar-calpainBy similarity
Short name:
muCANPBy similarity
Gene namesi
Name:CAPN1By similarity
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000207699‹1 – 302Calpain-1 catalytic subunitAdd BLAST›302

Post-translational modificationi

The N-terminus is blocked.1 Publication
Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PRIDEiP06815.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit CAPNS1.By similarity

Structurei

3D structure databases

ProteinModelPortaliP06815.
SMRiP06815.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini173 – 206EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini203 – 238EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini268 – 302EF-hand 3PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni‹1 – 114Domain IIIAdd BLAST›114
Regioni115 – 130LinkerAdd BLAST16
Regioni131 – 301Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012645.
InParanoidiP06815.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
InterProiView protein in InterPro
IPR033883. C2_III.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR036213. Calpain_III_sf.
IPR029643. CAPN1.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
PANTHERiPTHR10183:SF284. PTHR10183:SF284. 1 hit.
PfamiView protein in Pfam
PF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
SMARTiView protein in SMART
SM00720. calpain_III. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.

Sequencei

Sequence statusi: Fragment.

P06815-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
RESGCSFVLA LMQKHRRRER RFGRDMETIG FAVYEVPREL VGQPALHLKR
60 70 80 90 100
DFFLANASRA RSEQFINLRE VSTRFRLPPG EYVVVPSTFE PNKEGDFVLR
110 120 130 140 150
FFSEKRAGTQ ELDDQIQANL PDEQVLSAEE IDENFKALFR QLAGEDLEIS
160 170 180 190 200
VRELQTILNR ITSKHKDLRT KGFSMESCRS MVNLMDRDGN GKLGLVEFNI
210 220 230 240 250
LWNRIRNYLA IFRKFDLDKS GSMSAYEMRM AIESAGFKLN KKLYELIITR
260 270 280 290 300
YSEPDLAVDF DNFVCCLVRL ETMFRFFKTL DTDLDGVVTF DLFKWLQLTM

FA
Length:302
Mass (Da):35,275
Last modified:January 1, 1988 - v1
Checksum:i178BFEF4216C6EAB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13363 mRNA. Translation: AAA31456.1.
PIRiA24815.
UniGeneiOcu.1955.

Similar proteinsi

Entry informationi

Entry nameiCAN1_RABIT
AccessioniPrimary (citable) accession number: P06815
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 25, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families