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P06814

- CAN2_RABIT

UniProt

P06814 - CAN2_RABIT

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Protein
Calpain-2 catalytic subunit
Gene
CAPN2
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 7 calcium ions By similarity.

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei8 – 81 By similarity
Metal bindingi14 – 141Calcium 4 By similarity
Metal bindingi21 – 211Calcium 4 By similarity
Metal bindingi45 – 451Calcium 4; via carbonyl oxygen By similarity
Metal bindingi264 – 2641Calcium 5; via carbonyl oxygen By similarity
Metal bindingi267 – 2671Calcium 5 By similarity
Metal bindingi269 – 2691Calcium 5; via carbonyl oxygen By similarity
Metal bindingi274 – 2741Calcium 5 By similarity
Metal bindingi307 – 3071Calcium 6 By similarity
Metal bindingi309 – 3091Calcium 6 By similarity
Metal bindingi311 – 3111Calcium 6; via carbonyl oxygen By similarity
Metal bindingi313 – 3131Calcium 6; via carbonyl oxygen By similarity
Metal bindingi318 – 3181Calcium 6 By similarity
Metal bindingi337 – 3371Calcium 7 By similarity
Metal bindingi339 – 3391Calcium 7 By similarity
Metal bindingi341 – 3411Calcium 7; via carbonyl oxygen By similarity
Metal bindingi343 – 3431Calcium 7; via carbonyl oxygen By similarity
Metal bindingi348 – 3481Calcium 7 By similarity
Metal bindingi380 – 3801Calcium 1 By similarity
Metal bindingi383 – 3831Calcium 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi307 – 3181211 Publication
Add
BLAST
Calcium bindingi337 – 3481221 Publication
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cellular response to amino acid stimulus Source: UniProtKB
  2. proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 1749.

Protein family/group databases

MEROPSiC02.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Cytoplasm. Cell membrane
Note: Translocates to the plasma membrane upon Ca2+ binding.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. dendrite Source: UniProtKB
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 422›422Calpain-2 catalytic subunit
PRO_0000207704Add
BLAST

Proteomic databases

PRIDEiP06814.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000017627.

Structurei

3D structure databases

ProteinModelPortaliP06814.
SMRiP06814. Positions 1-422.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini‹1 – 66›66Calpain catalytic
Add
BLAST
Domaini294 – 32734EF-hand 1
Add
BLAST
Domaini324 – 35936EF-hand 2
Add
BLAST
Domaini389 – 42234EF-hand 3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 236170Domain III
Add
BLAST
Regioni237 – 25115Linker
Add
BLAST
Regioni252 – 422171Domain IV
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.
Contains 3 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P06814-1 [UniParc]FASTAAdd to Basket

« Hide

QKLIRIRNPW GEVEWTGRWN DNCPNWNTVD PEVRERLAER HEDGEFWMSF    50
SDFLRHYSRL EICNLTPDTL TSDTYKKWKL TKMDGNWRRG STAGGCRNYP 100
NTFWMNPQYV IKLEEEDEDQ EDGESGCTFL VGLIQKHRRR QRKMGEDMHT 150
IGFGIYEVPE ELRGQTNIHL GKNFFLTTRA RERSDTFINL REVLNRFKLP 200
PGEYILVPST FEPNKNGDFC VRVFSEKKAD YQAVDDEIEA DLEEADVSED 250
DIDDGFRRLF AQLAGEDAEI SAFELQNILR RVLAKRQDIK TDGLSIETCK 300
IMVDMLDSDG TGKLGLKEFY VLWTKIQKYQ KIYREIDVDR SGTMNSYEMR 350
KALEEAGFKL PCQLHEVIVA RFADDQLIID FDNFVRCLVR LETLFKIFKQ 400
LDPDNTGMIQ LDLISWLCFS VL 422
Length:422
Mass (Da):49,494
Last modified:January 1, 1988 - v1
Checksum:iAE4FA3C48A333C41
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13797 mRNA. Translation: AAA31455.1.
PIRiB24815.
UniGeneiOcu.1954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13797 mRNA. Translation: AAA31455.1 .
PIRi B24815.
UniGenei Ocu.1954.

3D structure databases

ProteinModelPortali P06814.
SMRi P06814. Positions 1-422.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000017627.

Protein family/group databases

MEROPSi C02.972.

Proteomic databases

PRIDEi P06814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi NOG327523.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.

Enzyme and pathway databases

BRENDAi 3.4.22.53. 1749.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and sequence analyses of cDNA clones for the large subunits of two isozymes of rabbit calcium-dependent protease."
    Emori Y., Kawasaki H., Sugihara H., Imajoh S., Kawashima S., Suzuki K.
    J. Biol. Chem. 261:9465-9471(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "E-F hand structure-domain of calcium-activated neutral protease (CANP) can bind Ca2+ ions."
    Minami Y., Emori Y., Kawasaki H., Suzuki K.
    J. Biochem. 101:889-895(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: CALCIUM-BINDING DATA.

Entry informationi

Entry nameiCAN2_RABIT
AccessioniPrimary (citable) accession number: P06814
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 3, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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