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Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+By similarityNote: Binds 7 Ca2+ ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei8PROSITE-ProRule annotation1
Metal bindingi14Calcium 4By similarity1
Metal bindingi21Calcium 4By similarity1
Metal bindingi45Calcium 4; via carbonyl oxygenBy similarity1
Metal bindingi264Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi267Calcium 5By similarity1
Metal bindingi269Calcium 5; via carbonyl oxygenBy similarity1
Metal bindingi274Calcium 5By similarity1
Metal bindingi307Calcium 6By similarity1
Metal bindingi309Calcium 6By similarity1
Metal bindingi311Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi313Calcium 6; via carbonyl oxygenBy similarity1
Metal bindingi318Calcium 6By similarity1
Metal bindingi337Calcium 7By similarity1
Metal bindingi339Calcium 7By similarity1
Metal bindingi341Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi343Calcium 7; via carbonyl oxygenBy similarity1
Metal bindingi348Calcium 7By similarity1
Metal bindingi380Calcium 1By similarity1
Metal bindingi383Calcium 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi307 – 31811 PublicationAdd BLAST12
Calcium bindingi337 – 34821 PublicationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 1749.

Protein family/group databases

MEROPSiC02.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000207704‹1 – 422Calpain-2 catalytic subunitAdd BLAST›422

Proteomic databases

PRIDEiP06814.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Interacts with CPEB3; this leads to cleavage of CPEB3.By similarity

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000017627.

Structurei

3D structure databases

ProteinModelPortaliP06814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini‹1 – 66Calpain catalyticPROSITE-ProRule annotationAdd BLAST›66
Domaini294 – 327EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini324 – 359EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini389 – 422EF-hand 3PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 236Domain IIIAdd BLAST170
Regioni237 – 251LinkerAdd BLAST15
Regioni252 – 422Domain IVAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP06814.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P06814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QKLIRIRNPW GEVEWTGRWN DNCPNWNTVD PEVRERLAER HEDGEFWMSF
60 70 80 90 100
SDFLRHYSRL EICNLTPDTL TSDTYKKWKL TKMDGNWRRG STAGGCRNYP
110 120 130 140 150
NTFWMNPQYV IKLEEEDEDQ EDGESGCTFL VGLIQKHRRR QRKMGEDMHT
160 170 180 190 200
IGFGIYEVPE ELRGQTNIHL GKNFFLTTRA RERSDTFINL REVLNRFKLP
210 220 230 240 250
PGEYILVPST FEPNKNGDFC VRVFSEKKAD YQAVDDEIEA DLEEADVSED
260 270 280 290 300
DIDDGFRRLF AQLAGEDAEI SAFELQNILR RVLAKRQDIK TDGLSIETCK
310 320 330 340 350
IMVDMLDSDG TGKLGLKEFY VLWTKIQKYQ KIYREIDVDR SGTMNSYEMR
360 370 380 390 400
KALEEAGFKL PCQLHEVIVA RFADDQLIID FDNFVRCLVR LETLFKIFKQ
410 420
LDPDNTGMIQ LDLISWLCFS VL
Length:422
Mass (Da):49,494
Last modified:January 1, 1988 - v1
Checksum:iAE4FA3C48A333C41
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13797 mRNA. Translation: AAA31455.1.
PIRiB24815.
UniGeneiOcu.1954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13797 mRNA. Translation: AAA31455.1.
PIRiB24815.
UniGeneiOcu.1954.

3D structure databases

ProteinModelPortaliP06814.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000017627.

Protein family/group databases

MEROPSiC02.972.

Proteomic databases

PRIDEiP06814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP06814.

Enzyme and pathway databases

BRENDAi3.4.22.53. 1749.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN2_RABIT
AccessioniPrimary (citable) accession number: P06814
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.