Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P06814

- CAN2_RABIT

UniProt

P06814 - CAN2_RABIT

Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' By similarity.By similarity

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 7 calcium ions.By similarity

    Enzyme regulationi

    Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei8 – 81PROSITE-ProRule annotation
    Metal bindingi14 – 141Calcium 4By similarity
    Metal bindingi21 – 211Calcium 4By similarity
    Metal bindingi45 – 451Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi264 – 2641Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi267 – 2671Calcium 5By similarity
    Metal bindingi269 – 2691Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi274 – 2741Calcium 5By similarity
    Metal bindingi307 – 3071Calcium 6By similarity
    Metal bindingi309 – 3091Calcium 6By similarity
    Metal bindingi311 – 3111Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi313 – 3131Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi318 – 3181Calcium 6By similarity
    Metal bindingi337 – 3371Calcium 7By similarity
    Metal bindingi339 – 3391Calcium 7By similarity
    Metal bindingi341 – 3411Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi343 – 3431Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi348 – 3481Calcium 7By similarity
    Metal bindingi380 – 3801Calcium 1By similarity
    Metal bindingi383 – 3831Calcium 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi307 – 318121Add
    BLAST
    Calcium bindingi337 – 348122Add
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to amino acid stimulus Source: UniProtKB
    2. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.4.22.53. 1749.

    Protein family/group databases

    MEROPSiC02.972.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-2 catalytic subunit (EC:3.4.22.53)
    Alternative name(s):
    Calcium-activated neutral proteinase 2
    Short name:
    CANP 2
    Calpain M-type
    Calpain-2 large subunit
    Millimolar-calpain
    Short name:
    M-calpain
    Gene namesi
    Name:CAPN2
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    Cytoplasm. Cell membrane
    Note: Translocates to the plasma membrane upon Ca2+ binding.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. dendrite Source: UniProtKB
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 422›422Calpain-2 catalytic subunitPRO_0000207704Add
    BLAST

    Proteomic databases

    PRIDEiP06814.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Interactioni

    Subunit structurei

    Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000017627.

    Structurei

    3D structure databases

    ProteinModelPortaliP06814.
    SMRiP06814. Positions 1-422.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini‹1 – 66›66Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini294 – 32734EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini324 – 35936EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini389 – 42234EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni67 – 236170Domain IIIAdd
    BLAST
    Regioni237 – 25115LinkerAdd
    BLAST
    Regioni252 – 422171Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG327523.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
    PfamiPF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P06814-1 [UniParc]FASTAAdd to Basket

    « Hide

    QKLIRIRNPW GEVEWTGRWN DNCPNWNTVD PEVRERLAER HEDGEFWMSF    50
    SDFLRHYSRL EICNLTPDTL TSDTYKKWKL TKMDGNWRRG STAGGCRNYP 100
    NTFWMNPQYV IKLEEEDEDQ EDGESGCTFL VGLIQKHRRR QRKMGEDMHT 150
    IGFGIYEVPE ELRGQTNIHL GKNFFLTTRA RERSDTFINL REVLNRFKLP 200
    PGEYILVPST FEPNKNGDFC VRVFSEKKAD YQAVDDEIEA DLEEADVSED 250
    DIDDGFRRLF AQLAGEDAEI SAFELQNILR RVLAKRQDIK TDGLSIETCK 300
    IMVDMLDSDG TGKLGLKEFY VLWTKIQKYQ KIYREIDVDR SGTMNSYEMR 350
    KALEEAGFKL PCQLHEVIVA RFADDQLIID FDNFVRCLVR LETLFKIFKQ 400
    LDPDNTGMIQ LDLISWLCFS VL 422
    Length:422
    Mass (Da):49,494
    Last modified:January 1, 1988 - v1
    Checksum:iAE4FA3C48A333C41
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13797 mRNA. Translation: AAA31455.1.
    PIRiB24815.
    UniGeneiOcu.1954.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13797 mRNA. Translation: AAA31455.1 .
    PIRi B24815.
    UniGenei Ocu.1954.

    3D structure databases

    ProteinModelPortali P06814.
    SMRi P06814. Positions 1-422.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000017627.

    Protein family/group databases

    MEROPSi C02.972.

    Proteomic databases

    PRIDEi P06814.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG327523.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.

    Enzyme and pathway databases

    BRENDAi 3.4.22.53. 1749.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
    Pfami PF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and sequence analyses of cDNA clones for the large subunits of two isozymes of rabbit calcium-dependent protease."
      Emori Y., Kawasaki H., Sugihara H., Imajoh S., Kawashima S., Suzuki K.
      J. Biol. Chem. 261:9465-9471(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "E-F hand structure-domain of calcium-activated neutral protease (CANP) can bind Ca2+ ions."
      Minami Y., Emori Y., Kawasaki H., Suzuki K.
      J. Biochem. 101:889-895(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: CALCIUM-BINDING DATA.

    Entry informationi

    Entry nameiCAN2_RABIT
    AccessioniPrimary (citable) accession number: P06814
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3