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Reviewed, UniProtKB/Swiss-Prot P06811 (LAC1_NEUCR)

Last modified June 16, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Laccase
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase
    Urishiol oxidase
    Diphenol oxidase
Gene names
Name: lacc
ORF Names: NCU04528
OrganismNeurospora crassa [Complete proteome]
Taxonomic identifier5141 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

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Protein attributes

Sequence length619 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Lignin degradation and detoxification of lignin-derived products By similarity.

Catalytic activity

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactor

Binds 4 copper ions per monomer By similarity.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the multicopper oxidase family.

Contains 3 plastocyanin-like domains.

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Ontologies

Keywords
   Biological processLignin degradation
   Cellular componentSecreted
   DomainRepeat
Signal
   LigandCopper
Metal-binding
   Molecular functionOxidoreductase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processlignin catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncopper ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

laccase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Propeptide22 – 4928
PRO_0000002921
Chain50 – 606557Laccase
PRO_0000002922
Propeptide607 – 61913
PRO_0000002923

Regions

Domain84 – 207124Plastocyanin-like 1
Domain216 – 373158Plastocyanin-like 2
Domain431 – 566136Plastocyanin-like 3

Sites

Metal binding1441Copper 1; type 2 By similarity
Metal binding1461Copper 2; type 3 By similarity
Metal binding1891Copper 2; type 3 By similarity
Metal binding1911Copper 3; type 3 By similarity
Metal binding4771Copper 4; type 1 By similarity
Metal binding4801Copper 1; type 2 By similarity
Metal binding4821Copper 3; type 3 By similarity
Metal binding5481Copper 3; type 3 By similarity
Metal binding5491Copper 4; type 1 By similarity
Metal binding5501Copper 2; type 3 By similarity
Metal binding5541Copper 4; type 1 By similarity

Amino acid modifications

Glycosylation1391N-linked (GlcNAc...) Potential
Glycosylation2821N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation3401N-linked (GlcNAc...) Potential
Glycosylation4221N-linked (GlcNAc...) Potential
Glycosylation4441N-linked (GlcNAc...) Potential

Natural variations

Natural variant171S → P in strain: Oak Ridge.
Natural variant211G → A in strain: Oak Ridge.
Natural variant981I → L in strain: TS.
Natural variant1141K → N in strain: TS.
Natural variant2921S → L in strain: Oak Ridge.
Natural variant3171F → L in strain: TS.
Natural variant3201V → I in strain: TS.
Natural variant346 – 3472SK → ND in strain: TS.
Natural variant3511D → T in strain: TS.
Natural variant3551H → K in strain: TS.
Natural variant3741Q → K in strain: TS.
Natural variant3791V → P in strain: TS.
Natural variant4391L → M in strain: TS.

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Sequences

Sequence LengthMass (Da)Tools
P06811-1 [UniParc].

Last modified November 28, 2006. Version 3.
Checksum: DD7C67B9135FC08A

FASTA61968,148
        10         20         30         40         50         60 
MKFLGIAALV AGLLAPSLVL GAPAPGTEGV NLLTPVDKRQ DSQAERYGGG GGGGCNSPTN 

        70         80         90        100        110        120 
RQCWSPGFNI NTDYELGTPN TGKTRRYKLT LTETDNWIGP DGVIKDKVMM VNDKIIGPTI 

       130        140        150        160        170        180 
QADWGDYIEI TVINKLKSNG TSIHWHGMHQ RNSNIQDGVN GVTECPIPPR GGSKVYRWRA 

       190        200        210        220        230        240 
TQYGTSWYHS HFSAQYGNGI VGPIVINGPA SANYDVDLGP FPLTDYYYDT ADRLVLLTQH 

       250        260        270        280        290        300 
AGPPPSNNVL FNGFAKHPTT GAGQYATVSL TKGKKHRLRL INTSVENHFQ LSLVNHSMTI 

       310        320        330        340        350        360 
ISADLVPVQP YKVDSLFLGV GQRYDVIIDA NQAVGNYWFN VTFGGSKLCG DSDNHYPAAI 

       370        380        390        400        410        420 
FRYQGAPKAL PTNQGVAPVD HQCLDLNDLK PVLQRSLNTN SIALNTGNTI PITLDGFVWR 

       430        440        450        460        470        480 
VNGTAININW NKPVLEYVLT GNTNYSQSDN IVQVEGVNQW KYWLIENDPD GAFSLPHPIH 

       490        500        510        520        530        540 
LHGHDFLILG RSPDVTAISQ TRYVFDPAVD MARLNGNNPT RRDTAMLPAK GWLLIAFRTD 

       550        560        570        580        590        600 
NPGSWLMHCH IAWHVSGGLS NQFLERAQDL RNSISPADKK AFNDNCDAWR AYFPDNAPFP 

       610 
KDDSGLRSGV KAREVKMKW

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References

« Hide 'large scale' references
[1]"Characterization of two allelic forms of Neurospora crassa laccase. Amino- and carboxyl-terminal processing of a precursor."
Germann U.A., Mueller G., Hunziker P.E., Lerch K.
J. Biol. Chem. 263:885-896(1988) [PubMed: 2961749] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: Oak Ridge and TS.
[2]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed: 12712197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"Isolation and partial nucleotide sequence of the laccase gene from Neurospora crassa: amino acid sequence homology of the protein to human ceruloplasmin."
Germann U.A., Lerch K.
Proc. Natl. Acad. Sci. U.S.A. 83:8854-8858(1986) [PubMed: 2947240] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 379-619.

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Cross-references

Sequence databases

M18333 Genomic DNA. Translation: AAA33591.1.
M18334 Genomic DNA. Translation: AAA33592.1.
AABX02000021 Genomic DNA. Translation: EAA27703.1.
M14554 Genomic DNA. Translation: AAA33590.1.
PIRKSNCLO. A28523.
KSNCLT. B28523.
RefSeqXP_956939.1.

3D structure databases

HSSPHSSP built from PDB template 1HFU based on UniProtKB Q9Y780.
SMRP06811. Positions 55-606.
ModBaseSearch...

Genome annotation databases

GeneID3873077.
KEGGncr:NCU04528.
NMPDRfig|5141.1.peg.1814.

Enzyme and pathway databases

BRENDA1.10.3.2. 266.

Family and domain databases

InterProIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 1 hit.
PfamPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
PROSITEPS00079. MULTICOPPER_OXIDASE1. 1 hit.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLAC1_NEUCR
AccessionPrimary (citable) accession number: P06811
Secondary accession number(s): P10574, Q7RV60
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 28, 2006
Last modified: June 16, 2009
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents