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P06811

- LAC1_NEUCR

UniProt

P06811 - LAC1_NEUCR

Protein

Laccase

Gene

lacc

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 3 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    Lignin degradation and detoxification of lignin-derived products.By similarity

    Catalytic activityi

    4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

    Cofactori

    Binds 4 copper ions per monomer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi144 – 1441Copper 1; type 2By similarity
    Metal bindingi146 – 1461Copper 2; type 3By similarity
    Metal bindingi189 – 1891Copper 2; type 3By similarity
    Metal bindingi191 – 1911Copper 3; type 3By similarity
    Metal bindingi477 – 4771Copper 4; type 1By similarity
    Metal bindingi480 – 4801Copper 1; type 2By similarity
    Metal bindingi482 – 4821Copper 3; type 3By similarity
    Metal bindingi548 – 5481Copper 3; type 3By similarity
    Metal bindingi549 – 5491Copper 4; type 1By similarity
    Metal bindingi550 – 5501Copper 2; type 3By similarity
    Metal bindingi554 – 5541Copper 4; type 1By similarity

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lignin catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase (EC:1.10.3.2)
    Alternative name(s):
    Benzenediol:oxygen oxidoreductase
    Diphenol oxidase
    Urishiol oxidase
    Gene namesi
    Name:lacc
    ORF Names:NCU04528
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 7, Linkage Group VII

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Propeptidei22 – 4928PRO_0000002921Add
    BLAST
    Chaini50 – 606557LaccasePRO_0000002922Add
    BLAST
    Propeptidei607 – 61913PRO_0000002923Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP06811.

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU04528.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP06811.
    SMRiP06811. Positions 55-606.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini84 – 207124Plastocyanin-like 1Add
    BLAST
    Domaini216 – 373158Plastocyanin-like 2Add
    BLAST
    Domaini431 – 566136Plastocyanin-like 3Add
    BLAST

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Curated
    Contains 3 plastocyanin-like domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG2132.
    HOGENOMiHOG000216827.
    OMAiTAININW.
    OrthoDBiEOG7H79B9.

    Family and domain databases

    Gene3Di2.60.40.420. 3 hits.
    InterProiIPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    [Graphical view]
    PfamiPF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 3 hits.
    PROSITEiPS00079. MULTICOPPER_OXIDASE1. 1 hit.
    PS00080. MULTICOPPER_OXIDASE2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P06811-1 [UniParc]FASTAAdd to Basket

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    MKFLGIAALV AGLLAPSLVL GAPAPGTEGV NLLTPVDKRQ DSQAERYGGG    50
    GGGGCNSPTN RQCWSPGFNI NTDYELGTPN TGKTRRYKLT LTETDNWIGP 100
    DGVIKDKVMM VNDKIIGPTI QADWGDYIEI TVINKLKSNG TSIHWHGMHQ 150
    RNSNIQDGVN GVTECPIPPR GGSKVYRWRA TQYGTSWYHS HFSAQYGNGI 200
    VGPIVINGPA SANYDVDLGP FPLTDYYYDT ADRLVLLTQH AGPPPSNNVL 250
    FNGFAKHPTT GAGQYATVSL TKGKKHRLRL INTSVENHFQ LSLVNHSMTI 300
    ISADLVPVQP YKVDSLFLGV GQRYDVIIDA NQAVGNYWFN VTFGGSKLCG 350
    DSDNHYPAAI FRYQGAPKAL PTNQGVAPVD HQCLDLNDLK PVLQRSLNTN 400
    SIALNTGNTI PITLDGFVWR VNGTAININW NKPVLEYVLT GNTNYSQSDN 450
    IVQVEGVNQW KYWLIENDPD GAFSLPHPIH LHGHDFLILG RSPDVTAISQ 500
    TRYVFDPAVD MARLNGNNPT RRDTAMLPAK GWLLIAFRTD NPGSWLMHCH 550
    IAWHVSGGLS NQFLERAQDL RNSISPADKK AFNDNCDAWR AYFPDNAPFP 600
    KDDSGLRSGV KAREVKMKW 619
    Length:619
    Mass (Da):68,148
    Last modified:November 28, 2006 - v3
    Checksum:iDD7C67B9135FC08A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti17 – 171S → P in strain: Oak Ridge.
    Natural varianti21 – 211G → A in strain: Oak Ridge.
    Natural varianti98 – 981I → L in strain: TS.
    Natural varianti114 – 1141K → N in strain: TS.
    Natural varianti292 – 2921S → L in strain: Oak Ridge.
    Natural varianti317 – 3171F → L in strain: TS.
    Natural varianti320 – 3201V → I in strain: TS.
    Natural varianti346 – 3472SK → ND in strain: TS.
    Natural varianti351 – 3511D → T in strain: TS.
    Natural varianti355 – 3551H → K in strain: TS.
    Natural varianti374 – 3741Q → K in strain: TS.
    Natural varianti379 – 3791V → P in strain: TS.
    Natural varianti439 – 4391L → M in strain: TS.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18333 Genomic DNA. Translation: AAA33591.1.
    M18334 Genomic DNA. Translation: AAA33592.1.
    CM002242 Genomic DNA. Translation: EAA27703.1.
    M14554 Genomic DNA. Translation: AAA33590.1.
    PIRiA28523. KSNCLO.
    B28523. KSNCLT.
    RefSeqiXP_956939.1. XM_951846.2.
    UniGeneiNcr.23952.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000005393; EFNCRP00000005387; EFNCRG00000005386.
    GeneIDi3873077.
    KEGGincr:NCU04528.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18333 Genomic DNA. Translation: AAA33591.1 .
    M18334 Genomic DNA. Translation: AAA33592.1 .
    CM002242 Genomic DNA. Translation: EAA27703.1 .
    M14554 Genomic DNA. Translation: AAA33590.1 .
    PIRi A28523. KSNCLO.
    B28523. KSNCLT.
    RefSeqi XP_956939.1. XM_951846.2.
    UniGenei Ncr.23952.

    3D structure databases

    ProteinModelPortali P06811.
    SMRi P06811. Positions 55-606.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU04528.1.

    Proteomic databases

    PRIDEi P06811.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000005393 ; EFNCRP00000005387 ; EFNCRG00000005386 .
    GeneIDi 3873077.
    KEGGi ncr:NCU04528.

    Phylogenomic databases

    eggNOGi COG2132.
    HOGENOMi HOG000216827.
    OMAi TAININW.
    OrthoDBi EOG7H79B9.

    Family and domain databases

    Gene3Di 2.60.40.420. 3 hits.
    InterProi IPR001117. Cu-oxidase.
    IPR011706. Cu-oxidase_2.
    IPR011707. Cu-oxidase_3.
    IPR002355. Cu_oxidase_Cu_BS.
    IPR008972. Cupredoxin.
    [Graphical view ]
    Pfami PF00394. Cu-oxidase. 1 hit.
    PF07731. Cu-oxidase_2. 1 hit.
    PF07732. Cu-oxidase_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 3 hits.
    PROSITEi PS00079. MULTICOPPER_OXIDASE1. 1 hit.
    PS00080. MULTICOPPER_OXIDASE2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of two allelic forms of Neurospora crassa laccase. Amino- and carboxyl-terminal processing of a precursor."
      Germann U.A., Mueller G., Hunziker P.E., Lerch K.
      J. Biol. Chem. 263:885-896(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: Oak Ridge and TS.
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "Isolation and partial nucleotide sequence of the laccase gene from Neurospora crassa: amino acid sequence homology of the protein to human ceruloplasmin."
      Germann U.A., Lerch K.
      Proc. Natl. Acad. Sci. U.S.A. 83:8854-8858(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 379-619.

    Entry informationi

    Entry nameiLAC1_NEUCR
    AccessioniPrimary (citable) accession number: P06811
    Secondary accession number(s): P10574, Q7RV60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 108 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3