Reviewed, UniProtKB/Swiss-Prot P06801 (MAOX_MOUSE)

Names and origin

Protein names

Recommended name:
    NADP-dependent malic enzyme
      Short name=NADP-ME
    EC=1.1.1.40
Alternative name(s):
    Malic enzyme 1

Gene names

Name:	Me1
Synonyms:	Mod-1, Mod1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to hormone stimulus Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB soluble fraction Inferred from direct assay. Source: MGI
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from sequence or structural similarity. Source: UniProtKB manganese ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	NADP-dependent malic enzyme		PRO_0000160193
Regions
	Nucleotide binding	301 – 318	18	NADP By similarity
Sites
	Active site	102	1	Proton donor By similarity
	Active site	173	1	Proton acceptor By similarity
	Metal binding	245	1	Divalent metal cation By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	269	1	Divalent metal cation By similarity
	Binding site	155	1	NADP By similarity
	Binding site	269	1	NADP By similarity
	Site	269	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06801-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 86B1761D04C2B51E	FASTA	572	63,999
	10 20 30 40 50 60 MEPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLNI HGLLPPCIIS QELQVLRIIK 70 80 90 100 110 120 NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSDVEKFMPI VYTPTVGLAC QQYSLAFRKP 130 140 150 160 170 180 RGLFISIHDK GHIASVLNAW PEDVVKAIVV TDGERILGLG DLGCNGMGIP VGKLALYTAC 190 200 210 220 230 240 GGVNPQQCLP ITLDVGTENE ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC 250 260 270 280 290 300 LIQFEDFANR NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ 310 320 330 340 350 360 GAGEAALGIA HLVVMAMEKE GLSKENARKK IWLVDSKGLI VKGRASLTEE KEVFAHEHEE 370 380 390 400 410 420 MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER PIIFALSSPT SKAECSADEC 430 440 450 460 470 480 YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG QGNNSYVFPG VALGVVACGL RHIDDKVFLT 490 500 510 520 530 540 TREVISQQVS DKHLQEGRLY PPLNTIRGVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV 550 560 570 SSQMYSTNYD QILPDCYPWP AEVQKIQTKV NQ

References

[1]	"Structure and expression of murine malic enzyme mRNA. Differentiation-dependent accumulation of two forms of malic enzyme mRNA in 3T3-L1 cells." Bagchi S., Wise L.S., Brown M.L., Bregman D., Sul H.S., Rubin C.S. J. Biol. Chem. 262:1558-1565(1987) [PubMed: 3805042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Regulation and structure of murine malic enzyme mRNA." Bagchi S., Wise L.S., Brown M.L., Sul H.S., Bregman D.B., Rubin C.S. Ann. N. Y. Acad. Sci. 478:77-92(1986) [PubMed: 3541755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 428-445, MASS SPECTROMETRY. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02652 mRNA. Translation: AAA39727.1. M26756 mRNA. Translation: AAA39489.1.
IPI	IPI00128857.
PIR	DEMSMX. A26683.
UniGene	Mm.148155
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
2-D gel databases
SWISS-2DPAGE	P06801.
REPRODUCTION-2DPAGE	P06801.
Genome annotation databases
Ensembl	ENSMUSG00000032418. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:97043. Me1.
Phylogenomic databases
HOGENOM	P06801.
HOVERGEN	P06801.
Enzyme and pathway databases
BRENDA	1.1.1.40. 244.
Gene expression databases
ArrayExpress	P06801.
Bgee	P06801.
CleanEx	MM_ME1.
GermOnline	ENSMUSG00000032418. Mus musculus.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

MAOX_MOUSE

Accession

Primary (citable) accession number: P06801

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)