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Protein

NADP-dependent malic enzyme

Gene

Me1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021Proton donorBy similarity
Binding sitei155 – 1551NADPBy similarity
Active sitei173 – 1731Proton acceptorBy similarity
Metal bindingi245 – 2451Divalent metal cationBy similarity
Metal bindingi246 – 2461Divalent metal cationBy similarity
Metal bindingi269 – 2691Divalent metal cationBy similarity
Binding sitei269 – 2691NADPBy similarity
Sitei269 – 2691Important for activityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi301 – 31818NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • malate metabolic process Source: UniProtKB
  • protein tetramerization Source: MGI
  • regulation of NADP metabolic process Source: MGI
  • response to hormone Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme (EC:1.1.1.40)
Short name:
NADP-ME
Alternative name(s):
Malic enzyme 1
Gene namesi
Name:Me1
Synonyms:Mod-1, Mod1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:97043. Me1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572NADP-dependent malic enzymePRO_0000160193Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei336 – 3361PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP06801.
MaxQBiP06801.
PaxDbiP06801.
PeptideAtlasiP06801.
PRIDEiP06801.

2D gel databases

REPRODUCTION-2DPAGEP06801.
SWISS-2DPAGEP06801.

PTM databases

iPTMnetiP06801.
PhosphoSiteiP06801.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032418.
CleanExiMM_ME1.
ExpressionAtlasiP06801. baseline and differential.
GenevisibleiP06801. MM.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi201464. 1 interaction.
IntActiP06801. 4 interactions.
MINTiMINT-1855632.
STRINGi10090.ENSMUSP00000034989.

Structurei

3D structure databases

ProteinModelPortaliP06801.
SMRiP06801. Positions 8-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
GeneTreeiENSGT00390000000754.
HOGENOMiHOG000042486.
HOVERGENiHBG000746.
InParanoidiP06801.
KOiK00029.
OMAiLNDPMYM.
OrthoDBiEOG091G04H9.
TreeFamiTF300537.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLNI HGLLPPCIIS
60 70 80 90 100
QELQVLRIIK NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSDVEKFMPI
110 120 130 140 150
VYTPTVGLAC QQYSLAFRKP RGLFISIHDK GHIASVLNAW PEDVVKAIVV
160 170 180 190 200
TDGERILGLG DLGCNGMGIP VGKLALYTAC GGVNPQQCLP ITLDVGTENE
210 220 230 240 250
ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC LIQFEDFANR
260 270 280 290 300
NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ
310 320 330 340 350
GAGEAALGIA HLVVMAMEKE GLSKENARKK IWLVDSKGLI VKGRASLTEE
360 370 380 390 400
KEVFAHEHEE MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER
410 420 430 440 450
PIIFALSNPT SKAECSAEQC YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG
460 470 480 490 500
QGNNSYVFPG VALGVVACGL RHIDDKVFLT TAEVISQQVS DKHLQEGRLY
510 520 530 540 550
PPLNTIRGVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV SSQMYSTNYD
560 570
QILPDCYPWP AEVQKIQTKV NQ
Length:572
Mass (Da):63,954
Last modified:July 27, 2011 - v2
Checksum:iD6BBF93FBEDAB1BE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti408 – 4081N → S in AAA39727 (PubMed:3805042).Curated
Sequence conflicti408 – 4081N → S in AAA39489 (PubMed:3541755).Curated
Sequence conflicti418 – 4192EQ → DE in AAA39727 (PubMed:3805042).Curated
Sequence conflicti418 – 4192EQ → DE in AAA39489 (PubMed:3541755).Curated
Sequence conflicti482 – 4821A → R in AAA39727 (PubMed:3805042).Curated
Sequence conflicti482 – 4821A → R in AAA39489 (PubMed:3541755).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02652 mRNA. Translation: AAA39727.1.
M26756 mRNA. Translation: AAA39489.1.
AK004980 mRNA. Translation: BAB23716.1.
AK077968 mRNA. Translation: BAC37086.1.
CCDSiCCDS23383.1.
PIRiA26683. DEMSMX.
RefSeqiNP_001185862.1. NM_001198933.1.
NP_032641.2. NM_008615.2.
UniGeneiMm.148155.

Genome annotation databases

EnsembliENSMUST00000034989; ENSMUSP00000034989; ENSMUSG00000032418.
GeneIDi17436.
KEGGimmu:17436.
UCSCiuc009qxs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02652 mRNA. Translation: AAA39727.1.
M26756 mRNA. Translation: AAA39489.1.
AK004980 mRNA. Translation: BAB23716.1.
AK077968 mRNA. Translation: BAC37086.1.
CCDSiCCDS23383.1.
PIRiA26683. DEMSMX.
RefSeqiNP_001185862.1. NM_001198933.1.
NP_032641.2. NM_008615.2.
UniGeneiMm.148155.

3D structure databases

ProteinModelPortaliP06801.
SMRiP06801. Positions 8-570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201464. 1 interaction.
IntActiP06801. 4 interactions.
MINTiMINT-1855632.
STRINGi10090.ENSMUSP00000034989.

PTM databases

iPTMnetiP06801.
PhosphoSiteiP06801.

2D gel databases

REPRODUCTION-2DPAGEP06801.
SWISS-2DPAGEP06801.

Proteomic databases

EPDiP06801.
MaxQBiP06801.
PaxDbiP06801.
PeptideAtlasiP06801.
PRIDEiP06801.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034989; ENSMUSP00000034989; ENSMUSG00000032418.
GeneIDi17436.
KEGGimmu:17436.
UCSCiuc009qxs.2. mouse.

Organism-specific databases

CTDi4199.
MGIiMGI:97043. Me1.

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
GeneTreeiENSGT00390000000754.
HOGENOMiHOG000042486.
HOVERGENiHBG000746.
InParanoidiP06801.
KOiK00029.
OMAiLNDPMYM.
OrthoDBiEOG091G04H9.
TreeFamiTF300537.

Miscellaneous databases

ChiTaRSiMe1. mouse.
PROiP06801.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032418.
CleanExiMM_ME1.
ExpressionAtlasiP06801. baseline and differential.
GenevisibleiP06801. MM.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAOX_MOUSE
AccessioniPrimary (citable) accession number: P06801
Secondary accession number(s): Q9DBF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.