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Protein

Receptor-type tyrosine-protein phosphatase C

Gene

Ptprc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity.By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei810SubstrateBy similarity1
Active sitei842Phosphocysteine intermediateBy similarity1
Binding sitei886SubstrateBy similarity1
Active sitei1157Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

  • heparan sulfate proteoglycan binding Source: MGI
  • heparin binding Source: MGI
  • protein kinase binding Source: UniProtKB
  • protein tyrosine phosphatase activity Source: MGI

GO - Biological processi

  • activation of MAPK activity Source: MGI
  • B cell differentiation Source: MGI
  • B cell proliferation Source: UniProtKB
  • B cell receptor signaling pathway Source: UniProtKB
  • bone marrow development Source: MGI
  • cell cycle phase transition Source: MGI
  • defense response to virus Source: MGI
  • dephosphorylation Source: UniProtKB
  • hematopoietic progenitor cell differentiation Source: MGI
  • heterotypic cell-cell adhesion Source: MGI
  • immunoglobulin biosynthetic process Source: MGI
  • leukocyte cell-cell adhesion Source: MGI
  • negative regulation of cell adhesion involved in substrate-bound cell migration Source: MGI
  • negative regulation of cytokine-mediated signaling pathway Source: UniProtKB
  • negative regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • negative regulation of protein autophosphorylation Source: MGI
  • negative regulation of protein kinase activity Source: UniProtKB
  • negative regulation of T cell mediated cytotoxicity Source: UniProtKB
  • negative thymic T cell selection Source: MGI
  • positive regulation of alpha-beta T cell proliferation Source: MGI
  • positive regulation of antigen receptor-mediated signaling pathway Source: UniProtKB
  • positive regulation of B cell proliferation Source: MGI
  • positive regulation of extrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of gamma-delta T cell differentiation Source: MGI
  • positive regulation of hematopoietic stem cell migration Source: MGI
  • positive regulation of humoral immune response mediated by circulating immunoglobulin Source: MGI
  • positive regulation of isotype switching to IgG isotypes Source: MGI
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of stem cell proliferation Source: MGI
  • positive regulation of T cell differentiation Source: MGI
  • positive regulation of T cell mediated cytotoxicity Source: MGI
  • positive regulation of T cell mediated immunity Source: MGI
  • positive regulation of T cell proliferation Source: UniProtKB
  • positive thymic T cell selection Source: MGI
  • protein dephosphorylation Source: UniProtKB
  • regulation of B cell differentiation Source: MGI
  • regulation of B cell receptor signaling pathway Source: MGI
  • regulation of cell cycle Source: UniProtKB
  • regulation of extrinsic apoptotic signaling pathway Source: MGI
  • regulation of humoral immune response mediated by circulating immunoglobulin Source: MGI
  • release of sequestered calcium ion into cytosol Source: UniProtKB
  • stem cell development Source: MGI
  • T cell differentiation Source: UniProtKB
  • T cell proliferation Source: MGI
  • T cell receptor signaling pathway Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase CCurated (EC:3.1.3.48)
Alternative name(s):
Leukocyte common antigen
Short name:
L-CA
Lymphocyte antigen 5
Short name:
Ly-5
T200
CD_antigen: CD45
Gene namesi
Name:PtprcImported
Synonyms:Ly-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componentsi: Chromosome 1, Unplaced

Organism-specific databases

MGIiMGI:97810. Ptprc.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 566ExtracellularSequence analysisAdd BLAST541
Transmembranei567 – 588HelicalSequence analysisAdd BLAST22
Topological domaini589 – 1293CytoplasmicSequence analysisAdd BLAST705

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Add BLAST25
ChainiPRO_000002547126 – 1293Receptor-type tyrosine-protein phosphatase CAdd BLAST1268

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi152N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi163N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi209N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi213N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi255N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi260N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi292N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi313N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi324N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi349N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi418N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi429N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi459N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi491N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei672PhosphotyrosineBy similarity1
Modified residuei964PhosphoserineCombined sources1
Modified residuei983PhosphoserineBy similarity1
Modified residuei986PhosphoserineBy similarity1
Modified residuei990PhosphoserineBy similarity1
Modified residuei993PhosphoserineBy similarity1
Modified residuei994PhosphoserineCombined sources1
Modified residuei998PhosphoserineBy similarity1
Modified residuei1229PhosphoserineBy similarity1
Modified residuei1267PhosphothreonineCombined sources1
Modified residuei1286PhosphoserineCombined sources1

Post-translational modificationi

Heavily N- and O-glycosylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP06800.
PaxDbiP06800.
PeptideAtlasiP06800.
PRIDEiP06800.

PTM databases

iPTMnetiP06800.
PhosphoSitePlusiP06800.
SwissPalmiP06800.

Expressioni

Developmental stagei

Expression is restricted to the hematopoietic compartment of development.

Gene expression databases

BgeeiENSMUSG00000026395.
CleanExiMM_PTPRC.
ExpressionAtlasiS4R1S4. baseline and differential.

Interactioni

Subunit structurei

Interacts with SKAP1. Interacts with DPP4; the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes (By similarity). Binds GANAB and PRKCSH.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LckP062403EBI-1672,EBI-1401

GO - Molecular functioni

  • heparan sulfate proteoglycan binding Source: MGI
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

IntActiP06800. 13 interactors.
MINTiP06800.
STRINGi10090.ENSMUSP00000027645.

Structurei

3D structure databases

ProteinModelPortaliP06800.
SMRiP06800.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini376 – 472Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST97
Domaini473 – 568Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96
Domaini642 – 901Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST260
Domaini933 – 1216Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST284

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni842 – 848Substrate bindingBy similarity7

Domaini

The first PTPase domain interacts with SKAP1.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118900.
HOVERGENiHBG000066.
InParanoidiP06800.
KOiK06478.
OMAiKHELEMS.
OrthoDBiEOG091G015A.
TreeFamiTF351829.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 2 hits.
3.90.190.10. 2 hits.
InterProiView protein in InterPro
IPR003961. FN3_dom.
IPR036116. FN3_sf.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR024739. PTP_recept_N.
IPR000242. PTPase_domain.
IPR016335. Ptprc.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
PfamiView protein in Pfam
PF12567. CD45. 1 hit.
PF12453. PTP_N. 1 hit.
PF00102. Y_phosphatase. 2 hits.
PIRSFiPIRSF002004. Leukocyte_common_antigen. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiView protein in SMART
SM00060. FN3. 2 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
SUPFAMiSSF49265. SSF49265. 2 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiView protein in PROSITE
PS50853. FN3. 2 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P06800-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMGLWLKLL AFGFALLDTE VFVTGQTPTP SDELSTTENA LLLPQSDPLP
60 70 80 90 100
ARTTESTPPS ISERGNGSSE TTYHPGVLST LLPHLSPQPD SQTPSAGGAD
110 120 130 140 150
TQTFSSQADN PTLTPAPGGG TDPPGVPGER TVPGTIPADT AFPVDTPSLA
160 170 180 190 200
RNSSAASPTH TSNVSTTDIS SGASLTTLTP STLGLASTDP PSTTIATTTK
210 220 230 240 250
QTCAAMFGNI TVNYTYESSN QTFKADLKDV QNAKCGNEDC ENVLNNLEEC
260 270 280 290 300
SQIKNISVSN DSCAPATTID LYVPPGTDKF SLHDCTPKEK ANTSICLEWK
310 320 330 340 350
TKNLDFRKCN SDNISYVLHC EPENNTKCIR RNTFIPERCQ LDNLRAQTNY
360 370 380 390 400
TCVAEILYRG VKLVKNVINV QTDLGIPETP KPSCGDPAAR KTLVSWPEPV
410 420 430 440 450
SKPESASKPH GYVLCYKNNS EKCKSLPNNV TSFEVESLKP YKYYEVSLLA
460 470 480 490 500
YVNGKIQRNG TAEKCNFHTK ADRPDKVNGM KTSRPTDNSI NVTCGPPYET
510 520 530 540 550
NGPKTFYILV VRSGGSFVTK YNKTNCQFYV DNLYYSTDYE FLVSFHNGVY
560 570 580 590 600
EGDSVIRNES TNFNAKALII FLVFLIIVTS IALLVVLYKI YDLRKKRSSN
610 620 630 640 650
LDEQQELVER DDEKQLMDVE PIHSDILLET YKRKIADEGR LFLAEFQSIP
660 670 680 690 700
RVFSKFPIKD ARKPHNQNKN RYVDILPYDY NRVELSEING DAGSTYINAS
710 720 730 740 750
YIDGFKEPRK YIAAQGPRDE TVDDFWRMIW EQKATVIVMV TRCEEGNRNK
760 770 780 790 800
CAEYWPSMEE GTRAFKDIVV TINDHKRCPD YIIQKLNVAH KKEKATGREV
810 820 830 840 850
THIQFTSWPD HGVPEDPHLL LKLRRRVNAF SNFFSGPIVV HCSAGVGRTG
860 870 880 890 900
TYIGIDAMLE GLEAEGKVDV YGYVVKLRRQ RCLMVQVEAQ YILIHQALVE
910 920 930 940 950
YNQFGETEVN LSELHSCLHN MKKRDPPSDP SPLEAEYQRL PSYRSWRTQH
960 970 980 990 1000
IGNQEENKKK NRNSNVVPYD FNRVPLKHEL EMSKESEPES DESSDDDSDS
1010 1020 1030 1040 1050
EETSKYINAS FVMSYWKPEM MIAAQGPLKE TIGDFWQMIF QRKVKVIVML
1060 1070 1080 1090 1100
TELVNGDQEV CAQYWGEGKQ TYGDMEVEMK DTNRASAYTL RTFELRHSKR
1110 1120 1130 1140 1150
KEPRTVYQYQ CTTWKGEELP AEPKDLVSMI QDLKQKLPKA SPEGMKYHKH
1160 1170 1180 1190 1200
ASILVHCRDG SQQTGLFCAL FNLLESAETE DVVDVFQVVK SLRKARPGVV
1210 1220 1230 1240 1250
CSYEQYQFLY DIIASIYPAQ NGQVKKTNSQ DKIEFHNEVD GGKQDANCVR
1260 1270 1280 1290
PDGPLNKAQE DSRGVGTPEP TNSAEEPEHA ANGSASPAPT QSS
Length:1,293
Mass (Da):144,837
Last modified:March 28, 2018 - v4
Checksum:i44C3D35121181B31
GO
Isoform 2 (identifier: P06800-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-75: Missing.

Show »
Length:1,250
Mass (Da):140,272
Checksum:iEDC7195D35923C29
GO
Isoform 3 (identifier: P06800-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-171: Missing.

Show »
Length:1,154
Mass (Da):130,855
Checksum:iBDFF3E12E270B07D
GO

Sequence cautioni

The sequence AAA39458 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAA39459 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAA40161 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence AAA40161 differs from that shown. Reason: Frameshift at position 969.Curated
The sequence AAA60449 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAB46374 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302K → E in AAA39459 (PubMed:1822988).Curated1
Sequence conflicti400 – 405VSKPES → ASKPDP in AAA39459 (PubMed:1822988).Curated6
Sequence conflicti478N → T in AAA39459 (PubMed:1822988).Curated1
Sequence conflicti530V → G in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti558N → S in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti590I → S in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti908E → Q in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti933L → R in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti955E → G in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti961N → K in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti966 – 969VVPY → CSID in AAA40161 (PubMed:2948186).Curated4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05941033 – 171Missing in isoform 3. Add BLAST139
Alternative sequenceiVSP_05941133 – 75Missing in isoform 2. Add BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14342 mRNA. Translation: AAA39458.1. Different initiation.
M92933 mRNA. Translation: AAA39459.1. Different initiation.
AC122903 Genomic DNA. No translation available.
AC159278 Genomic DNA. No translation available.
CH466520 Genomic DNA. Translation: EDL39542.1.
M17320 mRNA. Translation: AAA60449.1. Different initiation.
M23354 Genomic DNA. Translation: AAA39462.1.
M22456 Genomic DNA. Translation: AAB46374.1. Different initiation.
M11934 mRNA. Translation: AAA39461.1.
M23241 Genomic DNA. Translation: AAA39460.1.
M15174 mRNA. Translation: AAA40161.1. Sequence problems.
PIRiA23329.
A28334.
A28335.
I57644.
RefSeqiNP_001104786.2. NM_001111316.2.
NP_035340.3. NM_011210.4.
XP_006529320.1. XM_006529257.1.
XP_006529321.1. XM_006529258.3.
XP_006529322.1. XM_006529259.3.
UniGeneiMm.391573.

Genome annotation databases

EnsembliENSMUST00000182283; ENSMUSP00000138800; ENSMUSG00000026395.
ENSMUST00000183301; ENSMUSP00000138350; ENSMUSG00000026395.
GeneIDi19264.
KEGGimmu:19264.
UCSCiuc007cvh.3. mouse.
uc033fmd.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPTPRC_MOUSE
AccessioniPrimary (citable) accession number: P06800
Secondary accession number(s): E9QLT5
, Q61812, Q61813, Q61814, Q61815, Q78EF1, S4R1S4, S4R2V1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: March 28, 2018
Last modified: March 28, 2018
This is version 189 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-3 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome