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Protein

Receptor-type tyrosine-protein phosphatase C

Gene

Ptprc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity.By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei808SubstrateBy similarity1
Active sitei840Phosphocysteine intermediateBy similarity1
Binding sitei884SubstrateBy similarity1
Active sitei1155Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

  • heparan sulfate proteoglycan binding Source: MGI
  • heparin binding Source: MGI
  • protein kinase binding Source: UniProtKB
  • protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  • activation of MAPK activity Source: MGI
  • B cell differentiation Source: MGI
  • B cell proliferation Source: UniProtKB
  • B cell receptor signaling pathway Source: UniProtKB
  • bone marrow development Source: MGI
  • cell cycle phase transition Source: MGI
  • defense response to virus Source: UniProtKB
  • dephosphorylation Source: UniProtKB
  • hematopoietic progenitor cell differentiation Source: MGI
  • heterotypic cell-cell adhesion Source: MGI
  • immunoglobulin biosynthetic process Source: MGI
  • leukocyte cell-cell adhesion Source: MGI
  • negative regulation of cell adhesion involved in substrate-bound cell migration Source: MGI
  • negative regulation of cytokine-mediated signaling pathway Source: UniProtKB
  • negative regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • negative regulation of protein autophosphorylation Source: MGI
  • negative regulation of protein kinase activity Source: UniProtKB
  • negative regulation of T cell mediated cytotoxicity Source: UniProtKB
  • negative thymic T cell selection Source: MGI
  • positive regulation of alpha-beta T cell proliferation Source: MGI
  • positive regulation of antigen receptor-mediated signaling pathway Source: UniProtKB
  • positive regulation of B cell proliferation Source: MGI
  • positive regulation of extrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of gamma-delta T cell differentiation Source: MGI
  • positive regulation of hematopoietic stem cell migration Source: MGI
  • positive regulation of humoral immune response mediated by circulating immunoglobulin Source: MGI
  • positive regulation of isotype switching to IgG isotypes Source: MGI
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of stem cell proliferation Source: MGI
  • positive regulation of T cell differentiation Source: MGI
  • positive regulation of T cell mediated cytotoxicity Source: MGI
  • positive regulation of T cell mediated immunity Source: MGI
  • positive regulation of T cell proliferation Source: UniProtKB
  • positive thymic T cell selection Source: MGI
  • protein dephosphorylation Source: UniProtKB
  • regulation of B cell differentiation Source: MGI
  • regulation of B cell receptor signaling pathway Source: MGI
  • regulation of cell cycle Source: UniProtKB
  • regulation of extrinsic apoptotic signaling pathway Source: MGI
  • regulation of humoral immune response mediated by circulating immunoglobulin Source: MGI
  • release of sequestered calcium ion into cytosol Source: UniProtKB
  • stem cell development Source: MGI
  • T cell differentiation Source: UniProtKB
  • T cell proliferation Source: MGI
  • T cell receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase C (EC:3.1.3.48)
Alternative name(s):
Leukocyte common antigen
Short name:
L-CA
Lymphocyte antigen 5
Short name:
Ly-5
T200
CD_antigen: CD45
Gene namesi
Name:Ptprc
Synonyms:Ly-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97810. Ptprc.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 564ExtracellularSequence analysisAdd BLAST541
Transmembranei565 – 586HelicalSequence analysisAdd BLAST22
Topological domaini587 – 1291CytoplasmicSequence analysisAdd BLAST705

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000002547124 – 1291Receptor-type tyrosine-protein phosphatase CAdd BLAST1268

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...)Sequence analysis1
Glycosylationi150N-linked (GlcNAc...)Sequence analysis1
Glycosylationi161N-linked (GlcNAc...)Sequence analysis1
Glycosylationi207N-linked (GlcNAc...)Sequence analysis1
Glycosylationi211N-linked (GlcNAc...)Sequence analysis1
Glycosylationi218N-linked (GlcNAc...)Sequence analysis1
Glycosylationi253N-linked (GlcNAc...)Sequence analysis1
Glycosylationi258N-linked (GlcNAc...)Sequence analysis1
Glycosylationi290N-linked (GlcNAc...)Sequence analysis1
Glycosylationi311N-linked (GlcNAc...)Sequence analysis1
Glycosylationi322N-linked (GlcNAc...)Sequence analysis1
Glycosylationi347N-linked (GlcNAc...)Sequence analysis1
Glycosylationi416N-linked (GlcNAc...)Sequence analysis1
Glycosylationi427N-linked (GlcNAc...)Sequence analysis1
Glycosylationi457N-linked (GlcNAc...)Sequence analysis1
Glycosylationi489N-linked (GlcNAc...)Sequence analysis1
Modified residuei670PhosphotyrosineBy similarity1
Modified residuei962PhosphoserineCombined sources1
Modified residuei981PhosphoserineBy similarity1
Modified residuei984PhosphoserineBy similarity1
Modified residuei988PhosphoserineBy similarity1
Modified residuei991PhosphoserineBy similarity1
Modified residuei992PhosphoserineCombined sources1
Modified residuei996PhosphoserineBy similarity1
Modified residuei1227PhosphoserineBy similarity1
Modified residuei1265PhosphothreonineCombined sources1
Modified residuei1284PhosphoserineCombined sources1

Post-translational modificationi

Heavily N- and O-glycosylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP06800.
PaxDbiP06800.
PeptideAtlasiP06800.
PRIDEiP06800.

PTM databases

iPTMnetiP06800.
PhosphoSitePlusiP06800.
SwissPalmiP06800.

Expressioni

Developmental stagei

Expression is restricted to the hematopoietic compartment of development.

Gene expression databases

BgeeiENSMUSG00000026395.
CleanExiMM_PTPRC.

Interactioni

Subunit structurei

Interacts with SKAP1. Interacts with DPP4; the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes (By similarity). Binds GANAB and PRKCSH.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LckP062403EBI-1672,EBI-1401

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

IntActiP06800. 8 interactors.
MINTiMINT-188397.
STRINGi10090.ENSMUSP00000027645.

Structurei

3D structure databases

ProteinModelPortaliP06800.
SMRiP06800.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini374 – 470Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST97
Domaini471 – 566Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96
Domaini640 – 899Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST260
Domaini931 – 1214Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST284

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni840 – 846Substrate bindingBy similarity7

Domaini

The first PTPase domain interacts with SKAP1.By similarity

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
HOVERGENiHBG000066.
InParanoidiP06800.
TreeFamiTF351829.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 2 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR024739. PTP_recept_N.
IPR000242. PTPase_domain.
IPR016335. Ptprc.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF12567. CD45. 1 hit.
PF12453. PTP_N. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFiPIRSF002004. Leukocyte_common_antigen. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 2 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P06800-1) [UniParc]FASTAAdd to basket
Also known as: B220

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLWLKLLAF GFALLDTEVF VTGQTPTPSD ELSTTENALL LPQSDPLPAR
60 70 80 90 100
TTESTPPSIS ERGNGSSETT YHPGVLSTLL PHLSPQPDSQ TPSAGGADTQ
110 120 130 140 150
TFSSQADNPT LTPAPGGGTD PPGVPGERTV PGTIPADTAF PVDTPSLARN
160 170 180 190 200
SSAASPTHTS NVSTTDISSG ASLTTLTPST LGLASTDPPS TTIATTTKQT
210 220 230 240 250
CAAMFGNITV NYTYESSNQT FKADLKDVQN AKCGNEDCEN VLNNLEECSQ
260 270 280 290 300
IKNISVSNDS CAPATTIDLY VPPGTDKFSL HDCTPKEKAN TSICLEWKTK
310 320 330 340 350
NLDFRKCNSD NISYVLHCEP ENNTKCIRRN TFIPERCQLD NLRAQTNYTC
360 370 380 390 400
VAEILYRGVK LVKNVINVQT DLGIPETPKP SCGDPAARKT LVSWPEPVSK
410 420 430 440 450
PESASKPHGY VLCYKNNSEK CKSLPNNVTS FEVESLKPYK YYEVSLLAYV
460 470 480 490 500
NGKIQRNGTA EKCNFHTKAD RPDKVNGMKT SRPTDNSINV TCGPPYETNG
510 520 530 540 550
PKTFYILVVR SGGSFVTKYN KTNCQFYVDN LYYSTDYEFL VSFHNGVYEG
560 570 580 590 600
DSVIRNESTN FNAKALIIFL VFLIIVTSIA LLVVLYKIYD LRKKRSSNLD
610 620 630 640 650
EQQELVERDD EKQLMDVEPI HSDILLETYK RKIADEGRLF LAEFQSIPRV
660 670 680 690 700
FSKFPIKDAR KPHNQNKNRY VDILPYDYNR VELSEINGDA GSTYINASYI
710 720 730 740 750
DGFKEPRKYI AAQGPRDETV DDFWRMIWEQ KATVIVMVTR CEEGNRNKCA
760 770 780 790 800
EYWPSMEEGT RAFKDIVVTI NDHKRCPDYI IQKLNVAHKK EKATGREVTH
810 820 830 840 850
IQFTSWPDHG VPEDPHLLLK LRRRVNAFSN FFSGPIVVHC SAGVGRTGTY
860 870 880 890 900
IGIDAMLEGL EAEGKVDVYG YVVKLRRQRC LMVQVEAQYI LIHQALVEYN
910 920 930 940 950
QFGETEVNLS ELHSCLHNMK KRDPPSDPSP LEAEYQRLPS YRSWRTQHIG
960 970 980 990 1000
NQEENKKKNR NSNVVPYDFN RVPLKHELEM SKESEPESDE SSDDDSDSEE
1010 1020 1030 1040 1050
TSKYINASFV MSYWKPEMMI AAQGPLKETI GDFWQMIFQR KVKVIVMLTE
1060 1070 1080 1090 1100
LVNGDQEVCA QYWGEGKQTY GDMEVEMKDT NRASAYTLRT FELRHSKRKE
1110 1120 1130 1140 1150
PRTVYQYQCT TWKGEELPAE PKDLVSMIQD LKQKLPKASP EGMKYHKHAS
1160 1170 1180 1190 1200
ILVHCRDGSQ QTGLFCALFN LLESAETEDV VDVFQVVKSL RKARPGVVCS
1210 1220 1230 1240 1250
YEQYQFLYDI IASIYPAQNG QVKKTNSQDK IEFHNEVDGG KQDANCVRPD
1260 1270 1280 1290
GPLNKAQEDS RGVGTPEPTN SAEEPEHAAN GSASPAPTQS S
Length:1,291
Mass (Da):144,604
Last modified:July 27, 2011 - v3
Checksum:iED752FD195BA9643
GO
Isoform 2 (identifier: P06800-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     31-73: Missing.

Show »
Length:1,248
Mass (Da):140,040
Checksum:iD08281C0463EA174
GO
Isoform 3 (identifier: P06800-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     31-169: Missing.

Show »
Length:1,152
Mass (Da):130,622
Checksum:iF1AA6650E654CAAB
GO

Sequence cautioni

The sequence AAA39462 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti300K → E in AAA39459 (PubMed:1822988).Curated1
Sequence conflicti398V → A in AAA39459 (PubMed:1822988).Curated1
Sequence conflicti402 – 403ES → DP in AAA39459 (PubMed:1822988).Curated2
Sequence conflicti476N → T in AAA39459 (PubMed:1822988).Curated1
Sequence conflicti518 – 520KYN → NTT in AAA39458 (PubMed:2944116).Curated3
Sequence conflicti528V → G in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti556N → S in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti588I → S in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti906E → Q in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti931L → R in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti953E → G in AAA39458 (PubMed:2944116).Curated1
Sequence conflicti959N → K in AAA39458 (PubMed:2944116).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01244231 – 169Missing in isoform 3. 2 PublicationsAdd BLAST139
Alternative sequenceiVSP_01244131 – 73Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14342 mRNA. Translation: AAA39458.1.
M92933 mRNA. Translation: AAA39459.1.
AC122903 Genomic DNA. No translation available.
AC159278 Genomic DNA. No translation available.
M17320 mRNA. Translation: AAA60449.1.
M23354 Genomic DNA. Translation: AAA39462.1. Different initiation.
M22456 Genomic DNA. Translation: AAB46374.1.
M11934 mRNA. Translation: AAA39461.1.
M23241 Genomic DNA. Translation: AAA39460.1.
M15174 mRNA. Translation: AAA40161.1.
PIRiA23329.
A28334.
A28335.
I57644.
UniGeneiMm.391573.

Genome annotation databases

UCSCiuc007cvf.3. mouse. [P06800-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14342 mRNA. Translation: AAA39458.1.
M92933 mRNA. Translation: AAA39459.1.
AC122903 Genomic DNA. No translation available.
AC159278 Genomic DNA. No translation available.
M17320 mRNA. Translation: AAA60449.1.
M23354 Genomic DNA. Translation: AAA39462.1. Different initiation.
M22456 Genomic DNA. Translation: AAB46374.1.
M11934 mRNA. Translation: AAA39461.1.
M23241 Genomic DNA. Translation: AAA39460.1.
M15174 mRNA. Translation: AAA40161.1.
PIRiA23329.
A28334.
A28335.
I57644.
UniGeneiMm.391573.

3D structure databases

ProteinModelPortaliP06800.
SMRiP06800.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP06800. 8 interactors.
MINTiMINT-188397.
STRINGi10090.ENSMUSP00000027645.

PTM databases

iPTMnetiP06800.
PhosphoSitePlusiP06800.
SwissPalmiP06800.

Proteomic databases

MaxQBiP06800.
PaxDbiP06800.
PeptideAtlasiP06800.
PRIDEiP06800.

Protocols and materials databases

DNASUi19264.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007cvf.3. mouse. [P06800-1]

Organism-specific databases

MGIiMGI:97810. Ptprc.

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
HOVERGENiHBG000066.
InParanoidiP06800.
TreeFamiTF351829.

Miscellaneous databases

PROiP06800.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026395.
CleanExiMM_PTPRC.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 2 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR024739. PTP_recept_N.
IPR000242. PTPase_domain.
IPR016335. Ptprc.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF12567. CD45. 1 hit.
PF12453. PTP_N. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFiPIRSF002004. Leukocyte_common_antigen. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 2 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRC_MOUSE
AccessioniPrimary (citable) accession number: P06800
Secondary accession number(s): E9QLT5
, Q61812, Q61813, Q61814, Q61815, Q78EF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 178 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.