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P06798 (HXA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 148. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein Hox-A4
Alternative name(s):
Homeobox protein Hox-1.4
Homeobox protein MH-3
Gene names
Name:Hoxa4
Synonyms:Hox-1.4, Hoxa-4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.

Subcellular location

Nucleus.

Developmental stage

During development of the prepuberal testis high levels of HOXA4 transcripts were found at days 17, 24 and 30. The first day of HOXA4 expression was day 14. The activation of the HOXA4 gene in male germ cells seems to occur at the pachytene stage of meiotic prophase and its level of expression is stage-specific during embryogenesis.

Involvement in disease

Overexpression results in abnormal gut development (megacolon).

Sequence similarities

Belongs to the Antp homeobox family. Deformed subfamily.

Contains 1 homeobox DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Homeobox protein Hox-A4
PRO_0000200049

Regions

DNA binding180 – 23960Homeobox
Motif159 – 1646Antp-type hexapeptide

Experimental info

Sequence conflict971G → A in BAC36181. Ref.3
Sequence conflict971G → A in CAA47330. Ref.4
Sequence conflict117 – 1204VPLG → APPR in BAC36181. Ref.3
Sequence conflict1811K → E in CAA35228. Ref.5
Sequence conflict1811K → E in AAA37831. Ref.7
Sequence conflict2101Missing in AAA37831. Ref.7
Sequence conflict2691H → P Ref.1
Sequence conflict2691H → P Ref.2
Sequence conflict2691H → P in BAC36181. Ref.3
Sequence conflict272 – 2732HP → PE Ref.7
Sequence conflict279 – 2857TPIPSSI → HPFPPPYNLETWISFSHLCA LLISAPVLICSSLSKPETPK QNPTCWKP in CAA31889. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P06798 [UniParc].

Last modified July 15, 1999. Version 4.
Checksum: 475D48D6C6302A26

FASTA28530,468
        10         20         30         40         50         60 
MTMSSFLINS NYIEPKFPPF EEFAPHGGPG GGDGAVGGGP GYPRPQSAPH LPAPNPHAAR 

        70         80         90        100        110        120 
QPPAYYAPRA REPSYPGGLY PAPAAACPYA CRGASPGRPE QSPAPGAHPS PAPQPPVPLG 

       130        140        150        160        170        180 
HCAPGPTTPA VATGGSAPAC PLLLADQGPA GPKGKEPVVY PWMKKIHVSA VNSSYNGGEP 

       190        200        210        220        230        240 
KRSRTAYTRQ QVLELEKEFH FNRYLTRRRR IEIAHTLCLS ERQVKIWFQN RRMKWKKDHK 

       250        260        270        280 
LPNTKMRSSN TASAPAGPPG KAQTHSPHHH PHPLPGASTP IPSSI 

« Hide

References

« Hide 'large scale' references
[1]"Multiple levels of regulation exist for expression of the Hoxa-4 (Hox-1.4) gene in the mouse testis."
Viviano C.M., Galliot B., Wolgemuth D.J.
Cell. Mol. Biol. Res. 39:483-495(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Protein product of the somatic-type transcript of the Hoxa-4 (Hox-1.4) gene binds to homeobox consensus binding sites in its promoter and intron."
Wu K., Wolgemuth D.J.
J. Cell. Biochem. 52:449-462(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[4]"The mouse Hox-1.4 gene: primary structure, evidence for promoter activity and expression during development."
Galliot B., Dolle P., Vigneron M., Featherstone M.S., Baron A., Duboule D.
Development 107:343-359(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
[5]"Murine embryonic spinal cord and adult testis Hox-1.4 cDNAs are identical 3' to the homeo box."
Rubin M.R., Nguyen-Huu M.C.
DNA Seq. 1:329-334(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-199, NUCLEOTIDE SEQUENCE [MRNA] OF 180-285.
Strain: ICR.
Tissue: Spinal cord and Testis.
[6]"Differential expression of the c-abl proto-oncogene and the homeo box-containing gene Hox 1.4 during mouse spermatogenesis."
Duggal R.N., Zakeri Z.F., Ponzetto C., Wolgemuth D.J.
Ann. N. Y. Acad. Sci. 513:112-127(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 180-285.
[7]"A mouse homeo box gene is expressed in spermatocytes and embryos."
Rubin M.R., Toth L.E., Patel M.D., D'Eustachio P., Nguyen-Huu M.C.
Science 233:663-667(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 180-273.
[8]"Isolation of a mouse cDNA coding for a developmentally regulated, testis-specific transcript containing homeo box homology."
Wolgemuth D.J., Engelmyer E., Duggal R.N., Gizang-Ginsberg E., Mutter G.L., Ponzetto C., Viviano C., Zakeri Z.F.
EMBO J. 5:1229-1235(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 198-271.
[9]"Transgenic mice overexpressing the mouse homoeobox-containing gene Hox-1.4 exhibit abnormal gut development."
Wolgemuth D.J., Behringer R.R., Mostoller M.P., Brinster R.L., Palmiter R.D.
Nature 337:464-467(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: RESULTS OF OVEREXPRESSION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S70444 Genomic DNA. Translation: AAB30705.2.
S67058 Genomic DNA. Translation: AAB28662.2.
AK076098 mRNA. Translation: BAC36181.1.
X66861 Genomic DNA. Translation: CAA47330.1.
X13538 mRNA. Translation: CAA31889.1.
X17346 Genomic DNA. Translation: CAA35228.1.
M27432 mRNA. Translation: AAA16440.1.
M13813 mRNA. Translation: AAA37831.1.
PIRA43556.
RefSeqNP_032291.1. NM_008265.3.
UniGeneMm.439647.

3D structure databases

ProteinModelPortalP06798.
SMRP06798. Positions 153-240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200369. 1 interaction.

PTM databases

PhosphoSiteP06798.

Proteomic databases

PaxDbP06798.
PRIDEP06798.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000101395; ENSMUSP00000098943; ENSMUSG00000000942.
GeneID15401.
KEGGmmu:15401.
UCSCuc009byf.1. mouse.

Organism-specific databases

CTD3201.
MGIMGI:96176. Hoxa4.

Phylogenomic databases

eggNOGNOG257177.
GeneTreeENSGT00750000117269.
HOGENOMHOG000231179.
HOVERGENHBG016849.
InParanoidP06798.
KOK09304.
OrthoDBEOG780RP4.
PhylomeDBP06798.
TreeFamTF352857.

Gene expression databases

BgeeP06798.
CleanExMM_HOXA4.
GenevestigatorP06798.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017995. Homeobox_antennapedia.
IPR001827. Homeobox_Antennapedia_CS.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00025. ANTENNAPEDIA.
PR00024. HOMEOBOX.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00032. ANTENNAPEDIA. 1 hit.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288098.
PROP06798.
SOURCESearch...

Entry information

Entry nameHXA4_MOUSE
AccessionPrimary (citable) accession number: P06798
Secondary accession number(s): Q61684, Q64388, Q8BPE6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: July 15, 1999
Last modified: April 16, 2014
This is version 148 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Vertebrate homeotic Hox proteins

Nomenclature of vertebrate homeotic Hox proteins and list of entries