P06798 (HXA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein Hox-A4

Alternative name(s):
Homeobox protein Hox-1.4
Homeobox protein MH-3

Gene names

Name:	Hoxa4
Synonyms:	Hox-1.4, Hoxa-4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
Subcellular location	Nucleus.
Developmental stage	During development of the prepuberal testis high levels of HOXA4 transcripts were found at days 17, 24 and 30. The first day of HOXA4 expression was day 14. The activation of the HOXA4 gene in male germ cells seems to occur at the pachytene stage of meiotic prophase and its level of expression is stage-specific during embryogenesis.
Involvement in disease	Overexpression results in abnormal gut development (megacolon).
Sequence similarities	Belongs to the Antp homeobox family. Deformed subfamily. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterior/posterior pattern specification Inferred from mutant phenotype PubMed 7918106. Source: MGI embryonic skeletal system morphogenesis Inferred from mutant phenotype PubMed 7918106. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Traceable author statement PubMed 11900456. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Homeobox protein Hox-A4		PRO_0000200049
Regions
	DNA binding	180 – 239	60	Homeobox
	Motif	159 – 164	6	Antp-type hexapeptide
Experimental info
	Sequence conflict	97	1	G → A in BAC36181. Ref.3
	Sequence conflict	97	1	G → A in CAA47330. Ref.4
	Sequence conflict	117 – 120	4	VPLG → APPR in BAC36181. Ref.3
	Sequence conflict	181	1	K → E in CAA35228. Ref.5
	Sequence conflict	181	1	K → E in AAA37831. Ref.7
	Sequence conflict	210	1	Missing in AAA37831. Ref.7
	Sequence conflict	269	1	H → P Ref.1
	Sequence conflict	269	1	H → P Ref.2
	Sequence conflict	269	1	H → P in BAC36181. Ref.3
	Sequence conflict	272 – 273	2	HP → PE Ref.7
	Sequence conflict	279 – 285	7	TPIPSSI → HPFPPPYNLETWISFSHLCA LLISAPVLICSSLSKPETPK QNPTCWKP in CAA31889. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P06798 [UniParc]. Last modified July 15, 1999. Version 4. Checksum: 475D48D6C6302A26	FASTA	285	30,468
	10 20 30 40 50 60 MTMSSFLINS NYIEPKFPPF EEFAPHGGPG GGDGAVGGGP GYPRPQSAPH LPAPNPHAAR 70 80 90 100 110 120 QPPAYYAPRA REPSYPGGLY PAPAAACPYA CRGASPGRPE QSPAPGAHPS PAPQPPVPLG 130 140 150 160 170 180 HCAPGPTTPA VATGGSAPAC PLLLADQGPA GPKGKEPVVY PWMKKIHVSA VNSSYNGGEP 190 200 210 220 230 240 KRSRTAYTRQ QVLELEKEFH FNRYLTRRRR IEIAHTLCLS ERQVKIWFQN RRMKWKKDHK 250 260 270 280 LPNTKMRSSN TASAPAGPPG KAQTHSPHHH PHPLPGASTP IPSSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple levels of regulation exist for expression of the Hoxa-4 (Hox-1.4) gene in the mouse testis." Viviano C.M., Galliot B., Wolgemuth D.J. Cell. Mol. Biol. Res. 39:483-495(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Protein product of the somatic-type transcript of the Hoxa-4 (Hox-1.4) gene binds to homeobox consensus binding sites in its promoter and intron." Wu K., Wolgemuth D.J. J. Cell. Biochem. 52:449-462(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[4]	"The mouse Hox-1.4 gene: primary structure, evidence for promoter activity and expression during development." Galliot B., Dolle P., Vigneron M., Featherstone M.S., Baron A., Duboule D. Development 107:343-359(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
[5]	"Murine embryonic spinal cord and adult testis Hox-1.4 cDNAs are identical 3' to the homeo box." Rubin M.R., Nguyen-Huu M.C. DNA Seq. 1:329-334(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-199, NUCLEOTIDE SEQUENCE [MRNA] OF 180-285. Strain: ICR. Tissue: Spinal cord and Testis.
[6]	"Differential expression of the c-abl proto-oncogene and the homeo box-containing gene Hox 1.4 during mouse spermatogenesis." Duggal R.N., Zakeri Z.F., Ponzetto C., Wolgemuth D.J. Ann. N. Y. Acad. Sci. 513:112-127(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 180-285.
[7]	"A mouse homeo box gene is expressed in spermatocytes and embryos." Rubin M.R., Toth L.E., Patel M.D., D'Eustachio P., Nguyen-Huu M.C. Science 233:663-667(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 180-273.
[8]	"Isolation of a mouse cDNA coding for a developmentally regulated, testis-specific transcript containing homeo box homology." Wolgemuth D.J., Engelmyer E., Duggal R.N., Gizang-Ginsberg E., Mutter G.L., Ponzetto C., Viviano C., Zakeri Z.F. EMBO J. 5:1229-1235(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 198-271.
[9]	"Transgenic mice overexpressing the mouse homoeobox-containing gene Hox-1.4 exhibit abnormal gut development." Wolgemuth D.J., Behringer R.R., Mostoller M.P., Brinster R.L., Palmiter R.D. Nature 337:464-467(1989) [PubMed] [Europe PMC] [Abstract] Cited for: RESULTS OF OVEREXPRESSION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S70444 Genomic DNA. Translation: AAB30705.2. S67058 Genomic DNA. Translation: AAB28662.2. AK076098 mRNA. Translation: BAC36181.1. X66861 Genomic DNA. Translation: CAA47330.1. X13538 mRNA. Translation: CAA31889.1. X17346 Genomic DNA. Translation: CAA35228.1. M27432 mRNA. Translation: AAA16440.1. M13813 mRNA. Translation: AAA37831.1.
IPI	IPI00128156.
PIR	A43556.
RefSeq	NP_032291.1. NM_008265.3.
UniGene	Mm.439647.
3D structure databases
ProteinModelPortal	P06798.
SMR	P06798. Positions 153-240.
ModBase	Search...
PTM databases
PhosphoSite	P06798.
Proteomic databases
PaxDb	P06798.
PRIDE	P06798.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000101395; ENSMUSP00000098943; ENSMUSG00000000942.
GeneID	15401.
KEGG	mmu:15401.
UCSC	uc009byf.1. mouse.
Organism-specific databases
CTD	3201.
MGI	MGI:96176. Hoxa4.
Phylogenomic databases
eggNOG	NOG257177.
GeneTree	ENSGT00700000104090.
HOGENOM	HOG000231179.
HOVERGEN	HBG016849.
InParanoid	P06798.
KO	K09304.
OrthoDB	EOG4GTKF7.
Gene expression databases
Bgee	P06798.
CleanEx	MM_HOXA4.
Genevestigator	P06798.
GermOnline	ENSMUSG00000000942. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017995. Homeobox_antennapedia. IPR001827. Homeobox_Antennapedia_CS. IPR017970. Homeobox_CS. IPR020479. Homeobox_metazoa. IPR001356. Homeodomain. IPR009057. Homeodomain-like. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00025. ANTENNAPEDIA. PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00032. ANTENNAPEDIA. 1 hit. PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	288098.
SOURCE	Search...

Entry information

Entry name

HXA4_MOUSE

Accession

Primary (citable) accession number: P06798
Secondary accession number(s): Q61684, Q64388, Q8BPE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	July 15, 1999
Last modified:	April 3, 2013
This is version 141 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program