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Protein

Cathepsin L1

Gene

Ctsl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important for the overall degradation of proteins in lysosomes.

Catalytic activityi

Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381By similarity
Active sitei276 – 2761By similarity
Active sitei300 – 3001By similarity

GO - Molecular functioni

  • aminopeptidase activity Source: BHF-UCL
  • cysteine-type carboxypeptidase activity Source: MGI
  • cysteine-type endopeptidase activity Source: BHF-UCL
  • histone binding Source: BHF-UCL
  • peptide binding Source: BHF-UCL

GO - Biological processi

  • autophagic cell death Source: BHF-UCL
  • cell communication Source: BHF-UCL
  • cellular response to starvation Source: BHF-UCL
  • decidualization Source: BHF-UCL
  • hair follicle morphogenesis Source: MGI
  • male gonad development Source: BHF-UCL
  • multicellular organism aging Source: BHF-UCL
  • negative regulation of keratinocyte proliferation Source: MGI
  • nerve development Source: BHF-UCL
  • protein autoprocessing Source: MGI
  • protein processing Source: MGI
  • proteolysis Source: BHF-UCL
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
  • regulation of actin cytoskeleton reorganization Source: MGI
  • response to glucocorticoid Source: BHF-UCL
  • response to glucose Source: BHF-UCL
  • response to gonadotropin Source: BHF-UCL
  • response to organic cyclic compound Source: BHF-UCL
  • Sertoli cell differentiation Source: BHF-UCL
  • spermatogenesis Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14812.
BRENDAi3.4.22.15. 3474.
ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-2022090. Assembly of collagen fibrils and other multimeric structures.
R-MMU-2132295. MHC class II antigen presentation.

Protein family/group databases

MEROPSiC01.032.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin L1 (EC:3.4.22.15)
Alternative name(s):
Cathepsin L
Major excreted protein
Short name:
MEP
p39 cysteine proteinase
Cleaved into the following 2 chains:
Gene namesi
Name:Ctsl
Synonyms:Ctsl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:88564. Ctsl.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: Ensembl
  • cytoplasm Source: BHF-UCL
  • cytoplasmic vesicle Source: MGI
  • external side of plasma membrane Source: BHF-UCL
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • lysosome Source: BHF-UCL
  • microvillus Source: BHF-UCL
  • neuron projection Source: BHF-UCL
  • nucleolus Source: BHF-UCL
  • nucleus Source: BHF-UCL
  • perikaryon Source: BHF-UCL
  • secretory granule Source: Ensembl
  • vacuole Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5291.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Propeptidei18 – 11396Activation peptidePRO_0000026248Add
BLAST
Chaini114 – 288175Cathepsin L1 heavy chainPRO_0000026249Add
BLAST
Propeptidei289 – 2902PRO_0000026250
Chaini291 – 33444Cathepsin L1 light chainPRO_0000026251Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi135 ↔ 178By similarity
Disulfide bondi169 ↔ 211By similarity
Glycosylationi221 – 2211N-linked (GlcNAc...) (high mannose)2 Publications
Disulfide bondi269 ↔ 322Interchain (between heavy and light chains)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP06797.
PaxDbiP06797.
PeptideAtlasiP06797.
PRIDEiP06797.

PTM databases

iPTMnetiP06797.
PhosphoSiteiP06797.
SwissPalmiP06797.
UniCarbKBiP06797.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021477.
CleanExiMM_CTSL.
ExpressionAtlasiP06797. baseline and differential.
GenevisibleiP06797. MM.

Interactioni

Subunit structurei

Dimer of a heavy and a light chain linked by disulfide bonds.

GO - Molecular functioni

  • histone binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi198975. 2 interactions.
IntActiP06797. 5 interactions.
MINTiMINT-1863740.
STRINGi10090.ENSMUSP00000021933.

Chemistry

BindingDBiP06797.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MVVmodel-A22-334[»]
ProteinModelPortaliP06797.
SMRiP06797. Positions 21-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiP06797.
KOiK01365.
OMAiNGEYSNG.
OrthoDBiEOG091G0AKT.
PhylomeDBiP06797.
TreeFamiTF313739.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P06797-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLLLLLAVL CLGTALATPK FDQTFSAEWH QWKSTHRRLY GTNEEEWRRA
60 70 80 90 100
IWEKNMRMIQ LHNGEYSNGQ HGFSMEMNAF GDMTNEEFRQ VVNGYRHQKH
110 120 130 140 150
KKGRLFQEPL MLKIPKSVDW REKGCVTPVK NQGQCGSCWA FSASGCLEGQ
160 170 180 190 200
MFLKTGKLIS LSEQNLVDCS HAQGNQGCNG GLMDFAFQYI KENGGLDSEE
210 220 230 240 250
SYPYEAKDGS CKYRAEFAVA NDTGFVDIPQ QEKALMKAVA TVGPISVAMD
260 270 280 290 300
ASHPSLQFYS SGIYYEPNCS SKNLDHGVLL VGYGYEGTDS NKNKYWLVKN
310 320 330
SWGSEWGMEG YIKIAKDRDN HCGLATAASY PVVN
Length:334
Mass (Da):37,547
Last modified:November 1, 1990 - v2
Checksum:iFE6747043307AD98
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581M → I in AAA39984 (PubMed:2835398).Curated
Sequence conflicti58 – 581M → I in AAD32136 (PubMed:2835398).Curated
Sequence conflicti58 – 581M → I in AAD32137 (PubMed:2835398).Curated
Sequence conflicti58 – 581M → I in AAD32138 (PubMed:2835398).Curated
Sequence conflicti177 – 1771G → R (PubMed:3533924).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06086 mRNA. Translation: CAA29470.1.
J02583 mRNA. Translation: AAA37445.1.
M20495 Genomic DNA. Translation: AAA39984.1.
AF121837 mRNA. Translation: AAD32136.1.
AF121838 mRNA. Translation: AAD32137.1.
AF121839 mRNA. Translation: AAD32138.1.
BC068163 mRNA. Translation: AAH68163.1.
X04392 mRNA. Translation: CAA27980.1.
CCDSiCCDS26600.1.
PIRiS01177. KHMSL.
RefSeqiNP_034114.1. NM_009984.4.
XP_006517143.1. XM_006517080.1.
XP_006517144.1. XM_006517081.1.
UniGeneiMm.471755.
Mm.930.

Genome annotation databases

EnsembliENSMUST00000021933; ENSMUSP00000021933; ENSMUSG00000021477.
GeneIDi13039.
KEGGimmu:13039.
UCSCiuc007qyw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06086 mRNA. Translation: CAA29470.1.
J02583 mRNA. Translation: AAA37445.1.
M20495 Genomic DNA. Translation: AAA39984.1.
AF121837 mRNA. Translation: AAD32136.1.
AF121838 mRNA. Translation: AAD32137.1.
AF121839 mRNA. Translation: AAD32138.1.
BC068163 mRNA. Translation: AAH68163.1.
X04392 mRNA. Translation: CAA27980.1.
CCDSiCCDS26600.1.
PIRiS01177. KHMSL.
RefSeqiNP_034114.1. NM_009984.4.
XP_006517143.1. XM_006517080.1.
XP_006517144.1. XM_006517081.1.
UniGeneiMm.471755.
Mm.930.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MVVmodel-A22-334[»]
ProteinModelPortaliP06797.
SMRiP06797. Positions 21-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198975. 2 interactions.
IntActiP06797. 5 interactions.
MINTiMINT-1863740.
STRINGi10090.ENSMUSP00000021933.

Chemistry

BindingDBiP06797.
ChEMBLiCHEMBL5291.

Protein family/group databases

MEROPSiC01.032.

PTM databases

iPTMnetiP06797.
PhosphoSiteiP06797.
SwissPalmiP06797.
UniCarbKBiP06797.

Proteomic databases

EPDiP06797.
PaxDbiP06797.
PeptideAtlasiP06797.
PRIDEiP06797.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021933; ENSMUSP00000021933; ENSMUSG00000021477.
GeneIDi13039.
KEGGimmu:13039.
UCSCiuc007qyw.1. mouse.

Organism-specific databases

CTDi1514.
MGIiMGI:88564. Ctsl.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiP06797.
KOiK01365.
OMAiNGEYSNG.
OrthoDBiEOG091G0AKT.
PhylomeDBiP06797.
TreeFamiTF313739.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14812.
BRENDAi3.4.22.15. 3474.
ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-2022090. Assembly of collagen fibrils and other multimeric structures.
R-MMU-2132295. MHC class II antigen presentation.

Miscellaneous databases

PROiP06797.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021477.
CleanExiMM_CTSL.
ExpressionAtlasiP06797. baseline and differential.
GenevisibleiP06797. MM.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCATL1_MOUSE
AccessioniPrimary (citable) accession number: P06797
Secondary accession number(s): Q91UZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1990
Last modified: September 7, 2016
This is version 168 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.