Multidrug resistance protein 1B - Mus musculus (Mouse)

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P06795 (MDR1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Multidrug resistance protein 1B
EC=3.6.3.44

Alternative name(s):
ATP-binding cassette sub-family B member 1B
P-glycoprotein 1
CD_antigen=CD243

Gene names

Name:	Abcb1b
Synonyms:	Abcb1, Mdr1, Mdr1b, Pgy1, Pgy1-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1276 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Catalytic activity	ATP + H₂O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subunit structure	Interacts with PSMB5 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Post-translational modification	Several phosphorylated serine residues are present in the linker domain.
Miscellaneous	In mouse the MDR gene family includes three or more related but distinct cellular genes.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi membrane Inferred from Biological aspect of Ancestor. Source: RefGenome apical plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome integral to membrane Inferred from Biological aspect of Ancestor. Source: RefGenome intercellular canaliculus Inferred from direct assay PubMed 12068294. Source: MGI mitochondrion Inferred from direct assay PubMed 18614015. Source: MGI
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of substances Inferred from Biological aspect of Ancestor. Source: RefGenome xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1276

1276

Multidrug resistance protein 1B

PRO_0000093334

Regions

Topological domain

1 – 43

Cytoplasmic By similarity

Transmembrane

44 – 66

Helical; Potential

Topological domain

67 – 115

Extracellular By similarity

Transmembrane

116 – 136

Helical; Potential

Topological domain

137 – 185

Cytoplasmic By similarity

Transmembrane

186 – 207

Helical; Potential

Topological domain

208 – 214

Extracellular By similarity

Transmembrane

215 – 235

Helical; Potential

Topological domain

236 – 293

Cytoplasmic By similarity

Transmembrane

294 – 315

Helical; Potential

Topological domain

316 – 329

Extracellular By similarity

Transmembrane

330 – 351

Helical; Potential

Topological domain

352 – 709

358

Cytoplasmic By similarity

Transmembrane

710 – 730

Helical; Potential

Topological domain

731 – 754

Extracellular By similarity

Transmembrane

755 – 775

Helical; Potential

Topological domain

776 – 830

Cytoplasmic By similarity

Transmembrane

831 – 851

Helical; Potential

Topological domain

852

Extracellular By similarity

Transmembrane

853 – 872

Helical; Potential

Topological domain

873 – 932

Cytoplasmic By similarity

Transmembrane

933 – 955

Helical; Potential

Topological domain

956 – 971

Extracellular By similarity

Transmembrane

972 – 993

Helical; Potential

Topological domain

994 – 1276

283

Cytoplasmic By similarity

Domain

50 – 356

307

ABC transmembrane type-1 1

Domain

391 – 627

237

ABC transporter 1

Domain

709 – 998

290

ABC transmembrane type-1 2

Domain

1033 – 1271

239

ABC transporter 2

Nucleotide binding

426 – 433

ATP 1 By similarity

Nucleotide binding

1068 – 1075

ATP 2 By similarity

Amino acid modifications

Modified residue

641

Phosphotyrosine Ref.4

Modified residue

659

Phosphoserine Ref.4

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

103

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P06795 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 1804D0F011B0FF4E
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FASTA

1,276

140,994

        10         20         30         40         50         60 
MEFEENLKGR ADKNFSKMGK KSKKEKKEKK PAVGVFGMFR YADWLDKLCM ILGTLAAIIH 

        70         80         90        100        110        120 
GTLLPLLMLV FGNMTDSFTK AEASILPSIT NQSGPNSTLI ISNSSLEEEM AIYAYYYTGI 

       130        140        150        160        170        180 
GAGVLIVAYI QVSLWCLAAG RQIHKIRQKF FHAIMNQEIG WFDVHDVGEL NTRLTDDVSK 

       190        200        210        220        230        240 
INDGIGDKIG MFFQSITTFL AGFIIGFISG WKLTLVILAV SPLIGLSSAL WAKVLTSFTN 

       250        260        270        280        290        300 
KELQAYAKAG AVAEEVLAAI RTVIAFGGQQ KELERYNKNL EEAKNVGIKK AITASISIGI 

       310        320        330        340        350        360 
AYLLVYASYA LAFWYGTSLV LSNEYSIGEV LTVFFSILLG TFSIGHLAPN IEAFANARGA 

       370        380        390        400        410        420 
AFEIFKIIDN EPSIDSFSTK GYKPDSIMGN LEFKNVHFNY PSRSEVQILK GLNLKVKSGQ 

       430        440        450        460        470        480 
TVALVGNSGC GKSTTVQLMQ RLYDPLEGVV SIDGQDIRTI NVRYLREIIG VVSQEPVLFA 

       490        500        510        520        530        540 
TTIAENIRYG REDVTMDEIE KAVKEANAYD FIMKLPHQFD TLVGERGAQL SGGQKQRIAI 

       550        560        570        580        590        600 
ARALVRNPKI LLLDEATSAL DTESEAVVQA ALDKAREGRT TIVIAHRLST VRNADVIAGF 

       610        620        630        640        650        660 
DGGVIVEQGN HDELMREKGI YFKLVMTQTR GNEIEPGNNA YGSQSDTDAS ELTSEESKSP 

       670        680        690        700        710        720 
LIRRSIYRSV HRKQDQERRL SMKEAVDEDV PLVSFWRILN LNLSEWPYLL VGVLCAVING 

       730        740        750        760        770        780 
CIQPVFAIVF SRIVGVFSRD DDHETKRQNC NLFSLFFLVM GLISFVTYFF QGFTFGKAGE 

       790        800        810        820        830        840 
ILTKRVRYMV FKSMLRQDIS WFDDHKNSTG SLTTRLASDA SSVKGAMGAR LAVVTQNVAN 

       850        860        870        880        890        900 
LGTGVILSLV YGWQLTLLLV VIIPLIVLGG IIEMKLLSGQ ALKDKKQLEI SGKIATEAIE 

       910        920        930        940        950        960 
NFRTIVSLTR EQKFETMYAQ SLQVPYRNAM KKAHVFGITF SFTQAMMYFS YAACFRFGAY 

       970        980        990       1000       1010       1020 
LVAQQLMTFE NVMLVFSAVV FGAMAAGNTS SFAPDYAKAK VSASHIIRII EKTPEIDSYS 

      1030       1040       1050       1060       1070       1080 
TEGLKPTLLE GNVKFNGVQF NYPTRPNIPV LQGLSLEVKK GQTLALVGSS GCGKSTVVQL 

      1090       1100       1110       1120       1130       1140 
LERFYDPMAG SVFLDGKEIK QLNVQWLRAH LGIVSQEPIL FDCSIAENIA YGDNSRAVSH 

      1150       1160       1170       1180       1190       1200 
EEIVRAAKEA NIHQFIDSLP DKYNTRVGDK GTQLSGGQKQ RIAIARALVR QPHILLLDEA 

      1210       1220       1230       1240       1250       1260 
TSALDTESEK VVQEALDKAR EGRTCIVIAH RLSTIQNADL IVVIENGKVK EHGTHQQLLA 

      1270 
QKGIYFSMVQ AGAKRS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammalian multidrug resistance gene: complete cDNA sequence indicates strong homology to bacterial transport proteins." Gros P., Croop J., Housman D. Cell 47:371-380(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mammalian multidrug-resistance gene: correlation of exon organization with structural domains and duplication of an ancestral gene." Raymond M., Gros P. Proc. Natl. Acad. Sci. U.S.A. 86:6488-6492(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cell-specific activity of cis-acting regulatory elements in the promoter of the mouse multidrug resistance gene mdr1." Raymond M., Gros P. Mol. Cell. Biol. 10:6036-6040(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
[4]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-641 AND SER-659, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14757 mRNA. Translation: AAA79005.1. M60348 Genomic DNA. Translation: AAA39513.1.
IPI	IPI00128152.
PIR	DVMS1. A33719.
RefSeq	NP_035205.1. NM_011075.2.
UniGene	Mm.146649. Mm.389059.
3D structure databases
ProteinModelPortal	P06795.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000009058.
PTM databases
PhosphoSite	P06795.
Proteomic databases
PaxDb	P06795.
PRIDE	P06795.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000009058; ENSMUSP00000009058; ENSMUSG00000028970.
GeneID	18669.
KEGG	mmu:18669.
Organism-specific databases
CTD	18669.
MGI	MGI:97568. Abcb1b.
Phylogenomic databases
eggNOG	COG1132.
GeneTree	ENSGT00530000062896.
HOVERGEN	HBG080809.
InParanoid	P06795.
KO	K05658.
OMA	ACIPPSI.
OrthoDB	EOG42FSGR.
Enzyme and pathway databases
SABIO-RK	P06795.
Gene expression databases
ArrayExpress	P06795.
Bgee	P06795.
Genevestigator	P06795.
GermOnline	ENSMUSG00000028970. Mus musculus.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 2 hits.
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P06795.
ChEMBL	CHEMBL3467.
NextBio	294678.
SOURCE	Search...

Entry information

Entry name

MDR1B_MOUSE

Accession

Primary (citable) accession number: P06795

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents