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Reviewed, UniProtKB/Swiss-Prot P06795 (MDR1_MOUSE)

Last modified November 3, 2009. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Multidrug resistance protein 1
    EC=3.6.3.44
Alternative name(s):
    ATP-binding cassette sub-family B member 1
    P-glycoprotein 1
    CD_antigen=CD243
Gene names
Name: Abcb1
Synonyms: Abcb1b, Mdr1, Mdr1b, Pgy1, Pgy1-1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length1276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Membrane; Multi-pass membrane protein.

Post-translational modification

Several phosphorylated serine residues are present in the linker domain. Ref.4

Miscellaneous

In mouse the MDR gene family includes three or more related but distinct cellular genes.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

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Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   DomainRepeat
Transmembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
Phosphoprotein
Gene Ontology (GO)
   Biological processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

intercellular canaliculus

Inferred from direct assay. Source: MGI

mitochondrion

Inferred from direct assay. Source: MGI

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12761276Multidrug resistance protein 1
PRO_0000093334

Regions

Topological domain1 – 4747Cytoplasmic Potential
Transmembrane48 – 7124 Potential
Transmembrane119 – 13921 Potential
Transmembrane199 – 21921 Potential
Transmembrane296 – 31621 Potential
Transmembrane327 – 34721 Potential
Topological domain348 – 709362Cytoplasmic Potential
Transmembrane710 – 73021 Potential
Transmembrane755 – 77521 Potential
Transmembrane831 – 85121 Potential
Transmembrane855 – 88228 Potential
Transmembrane935 – 95521 Potential
Transmembrane966 – 98621 Potential
Topological domain987 – 1276290Cytoplasmic Potential
Domain50 – 356307ABC transmembrane type-1 1
Domain391 – 627237ABC transporter 1
Domain709 – 998290ABC transmembrane type-1 2
Domain1033 – 1271239ABC transporter 2
Nucleotide binding426 – 4338ATP 1 By similarity
Nucleotide binding1068 – 10758ATP 2 By similarity

Amino acid modifications

Modified residue6411Phosphotyrosine Ref.4
Modified residue6591Phosphoserine Ref.4
Glycosylation731N-linked (GlcNAc...) Potential
Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation1031N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
P06795-1 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 1804D0F011B0FF4E

FASTA1,276140,994
        10         20         30         40         50         60 
MEFEENLKGR ADKNFSKMGK KSKKEKKEKK PAVGVFGMFR YADWLDKLCM ILGTLAAIIH 

        70         80         90        100        110        120 
GTLLPLLMLV FGNMTDSFTK AEASILPSIT NQSGPNSTLI ISNSSLEEEM AIYAYYYTGI 

       130        140        150        160        170        180 
GAGVLIVAYI QVSLWCLAAG RQIHKIRQKF FHAIMNQEIG WFDVHDVGEL NTRLTDDVSK 

       190        200        210        220        230        240 
INDGIGDKIG MFFQSITTFL AGFIIGFISG WKLTLVILAV SPLIGLSSAL WAKVLTSFTN 

       250        260        270        280        290        300 
KELQAYAKAG AVAEEVLAAI RTVIAFGGQQ KELERYNKNL EEAKNVGIKK AITASISIGI 

       310        320        330        340        350        360 
AYLLVYASYA LAFWYGTSLV LSNEYSIGEV LTVFFSILLG TFSIGHLAPN IEAFANARGA 

       370        380        390        400        410        420 
AFEIFKIIDN EPSIDSFSTK GYKPDSIMGN LEFKNVHFNY PSRSEVQILK GLNLKVKSGQ 

       430        440        450        460        470        480 
TVALVGNSGC GKSTTVQLMQ RLYDPLEGVV SIDGQDIRTI NVRYLREIIG VVSQEPVLFA 

       490        500        510        520        530        540 
TTIAENIRYG REDVTMDEIE KAVKEANAYD FIMKLPHQFD TLVGERGAQL SGGQKQRIAI 

       550        560        570        580        590        600 
ARALVRNPKI LLLDEATSAL DTESEAVVQA ALDKAREGRT TIVIAHRLST VRNADVIAGF 

       610        620        630        640        650        660 
DGGVIVEQGN HDELMREKGI YFKLVMTQTR GNEIEPGNNA YGSQSDTDAS ELTSEESKSP 

       670        680        690        700        710        720 
LIRRSIYRSV HRKQDQERRL SMKEAVDEDV PLVSFWRILN LNLSEWPYLL VGVLCAVING 

       730        740        750        760        770        780 
CIQPVFAIVF SRIVGVFSRD DDHETKRQNC NLFSLFFLVM GLISFVTYFF QGFTFGKAGE 

       790        800        810        820        830        840 
ILTKRVRYMV FKSMLRQDIS WFDDHKNSTG SLTTRLASDA SSVKGAMGAR LAVVTQNVAN 

       850        860        870        880        890        900 
LGTGVILSLV YGWQLTLLLV VIIPLIVLGG IIEMKLLSGQ ALKDKKQLEI SGKIATEAIE 

       910        920        930        940        950        960 
NFRTIVSLTR EQKFETMYAQ SLQVPYRNAM KKAHVFGITF SFTQAMMYFS YAACFRFGAY 

       970        980        990       1000       1010       1020 
LVAQQLMTFE NVMLVFSAVV FGAMAAGNTS SFAPDYAKAK VSASHIIRII EKTPEIDSYS 

      1030       1040       1050       1060       1070       1080 
TEGLKPTLLE GNVKFNGVQF NYPTRPNIPV LQGLSLEVKK GQTLALVGSS GCGKSTVVQL 

      1090       1100       1110       1120       1130       1140 
LERFYDPMAG SVFLDGKEIK QLNVQWLRAH LGIVSQEPIL FDCSIAENIA YGDNSRAVSH 

      1150       1160       1170       1180       1190       1200 
EEIVRAAKEA NIHQFIDSLP DKYNTRVGDK GTQLSGGQKQ RIAIARALVR QPHILLLDEA 

      1210       1220       1230       1240       1250       1260 
TSALDTESEK VVQEALDKAR EGRTCIVIAH RLSTIQNADL IVVIENGKVK EHGTHQQLLA 

      1270 
QKGIYFSMVQ AGAKRS

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References

« Hide 'large scale' references
[1]"Mammalian multidrug resistance gene: complete cDNA sequence indicates strong homology to bacterial transport proteins."
Gros P., Croop J., Housman D.
Cell 47:371-380(1986) [PubMed: 3768958] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Mammalian multidrug-resistance gene: correlation of exon organization with structural domains and duplication of an ancestral gene."
Raymond M., Gros P.
Proc. Natl. Acad. Sci. U.S.A. 86:6488-6492(1989) [PubMed: 2570420] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"Cell-specific activity of cis-acting regulatory elements in the promoter of the mouse multidrug resistance gene mdr1."
Raymond M., Gros P.
Mol. Cell. Biol. 10:6036-6040(1990) [PubMed: 2248681] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-641 AND SER-659, MASS SPECTROMETRY.
Tissue: Macrophage.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M14757 mRNA. Translation: AAA79005.1.
M60348 Genomic DNA. Translation: AAA39513.1.
IPIIPI00128152.
PIRDVMS1. A33719.
RefSeqNP_035205.1.
UniGeneMm.146649
Mm.389059

3D structure databases

HSSPHSSP built from PDB template 1MT0 based on UniProtKB P08716.
ModBaseSearch...

Protein-protein interaction databases

STRINGP06795.

Proteomic databases

PRIDEP06795.

Genome annotation databases

EnsemblENSMUST00000009058; ENSMUSP00000009058; ENSMUSG00000028970; Mus musculus. [Genome view]
GeneID18669.
KEGGmmu:18669.
UCSCuc008wkp.1. mouse.

Organism-specific databases

CTD18669.
MGIMGI:97568. Abcb1b.

Phylogenomic databases

HOGENOMP06795.
HOVERGENP06795.
OMACAVINGC.

Enzyme and pathway databases

BRENDA3.6.3.44. 244.

Gene expression databases

ArrayExpressP06795.
BgeeP06795.
GenevestigatorP06795.
GermOnlineENSMUSG00000028970. Mus musculus.

Family and domain databases

InterProIPR001140. ABC_TM_transpt.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
ProDomPD000006. ABC_transporter. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio294678.
SOURCESearch...

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Entry information

Entry nameMDR1_MOUSE
AccessionPrimary (citable) accession number: P06795
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 3, 2009
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents