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Protein

Replication protein E1

Gene

E1

Organism
Human papillomavirus type 18
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi484 – 4918ATPUniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • ATP-dependent DNA helicase activity Source: BHF-UCL
  • DNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  • DNA replication Source: UniProtKB-HAMAP
  • viral genome replication Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein E1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase E1UniRule annotation
Gene namesi
Name:E1UniRule annotation
OrganismiHuman papillomavirus type 18
Taxonomic identifieri333761 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeAlphapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009109 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

GO - Cellular componenti

  • host cell cytoplasm Source: BHF-UCL
  • host cell nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi225 – 2262SF → PS: Complete loss of SMARCB1/INI1 binding. 1 Publication
Mutagenesisi305 – 3051L → K: Complete loss of SMARCB1/INI1 binding. 1 Publication
Mutagenesisi395 – 3962NA → HE: Complete loss of SMARCB1/INI1 binding. 1 Publication
Mutagenesisi418 – 4192HY → EH: Complete loss of SMARCB1/INI1 binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Replication protein E1PRO_0000133116Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei110 – 1101Phosphoserine; by hostUniRule annotation
Cross-linki565 – 565Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation

Post-translational modificationi

Phosphorylated.UniRule annotation
Sumoylated.UniRule annotation

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.Interacts with human SMARCB1/INI1 protein, stimulating viral replication.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
IFIT1P099148EBI-7015660,EBI-745117From a different organism.
SMARCB1Q12824-15EBI-7015660,EBI-7015645From a different organism.

Protein-protein interaction databases

IntActiP06789. 2 interactions.
MINTiMINT-94927.

Structurei

Secondary structure

1
657
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi211 – 22212Combined sources
Helixi226 – 2283Combined sources
Beta strandi240 – 25011Combined sources
Helixi252 – 2565Combined sources
Helixi258 – 2625Combined sources
Helixi263 – 2653Combined sources
Beta strandi266 – 27611Combined sources
Beta strandi279 – 29214Combined sources
Helixi294 – 30512Combined sources
Helixi309 – 3113Combined sources
Beta strandi312 – 3154Combined sources
Helixi322 – 33312Combined sources
Beta strandi337 – 3415Combined sources
Helixi345 – 3506Combined sources
Helixi429 – 43810Combined sources
Helixi447 – 4559Combined sources
Helixi460 – 47213Combined sources
Beta strandi478 – 4847Combined sources
Helixi486 – 4883Combined sources
Helixi490 – 50112Combined sources
Beta strandi511 – 5133Combined sources
Helixi515 – 5206Combined sources
Beta strandi524 – 5318Combined sources
Helixi533 – 54210Combined sources
Helixi544 – 5485Combined sources
Beta strandi552 – 5543Combined sources
Beta strandi557 – 5593Combined sources
Beta strandi562 – 5643Combined sources
Beta strandi569 – 5757Combined sources
Beta strandi579 – 5824Combined sources
Helixi584 – 5874Combined sources
Beta strandi591 – 5944Combined sources
Helixi613 – 62311Combined sources
Helixi625 – 6273Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R9WX-ray1.80A210-354[»]
1TUEX-ray2.10A/D/F/H/K/M428-631[»]
ProteinModelPortaliP06789.
SMRiP06789. Positions 210-354, 360-629.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06789.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini458 – 608151SF3 helicaseUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 444298SMARCB1/INI1-bindingAdd
BLAST
Regioni193 – 359167DNA-binding regionUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi86 – 883Nuclear localization signalUniRule annotation
Motifi109 – 11810Nuclear export signalUniRule annotation

Sequence similaritiesi

Belongs to the papillomaviridae E1 protein family.UniRule annotation
Contains 1 SF3 helicase domain.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04000. PPV_E1. 1 hit.
InterProiIPR001177. DNA_helicase_E1_C_Papillomavir.
IPR014000. DNA_helicase_E1_N_Papillomavir.
IPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR016393. Rep_E1_papillomaV.
[Graphical view]
PfamiPF00519. PPV_E1_C. 1 hit.
PF00524. PPV_E1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003383. Rep_E1_papillomaV. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51206. SF3_HELICASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPEGTDGE GTGCNGWFYV QAIVDKKTGD VISDDEDENA TDTGSDMVDF
60 70 80 90 100
IDTQGTFCEQ AELETAQALF HAQEVHNDAQ VLHVLKRKFA GGSTENSPLG
110 120 130 140 150
ERLEVDTELS PRLQEISLNS GQKKAKRRLF TISDSGYGCS EVEATQIQVT
160 170 180 190 200
TNGEHGGNVC SGGSTEAIDN GGTEGNNSSV DGTSDNSNIE NVNPQCTIAQ
210 220 230 240 250
LKDLLKVNNK QGAMLAVFKD TYGLSFTDLV RNFKSDKTTC TDWVTAIFGV
260 270 280 290 300
NPTIAEGFKT LIQPFILYAH IQCLDCKWGV LILALLRYKC GKSRLTVAKG
310 320 330 340 350
LSTLLHVPET CMLIQPPKLR SSVAALYWYR TGISNISEVM GDTPEWIQRL
360 370 380 390 400
TIIQHGIDDS NFDLSEMVQW AFDNELTDES DMAFEYALLA DSNSNAAAFL
410 420 430 440 450
KSNCQAKYLK DCATMCKHYR RAQKRQMNMS QWIRFRCSKI DEGGDWRPIV
460 470 480 490 500
QFLRYQQIEF ITFLGALKSF LKGTPKKNCL VFCGPANTGK SYFGMSFIHF
510 520 530 540 550
IQGAVISFVN STSHFWLEPL TDTKVAMLDD ATTTCWTYFD TYMRNALDGN
560 570 580 590 600
PISIDRKHKP LIQLKCPPIL LTTNIHPAKD NRWPYLESRI TVFEFPNAFP
610 620 630 640 650
FDKNGNPVYE INDKNWKCFF ERTWSRLDLH EEEEDADTEG NPFGTFKLRA

GQNHRPL
Length:657
Mass (Da):73,736
Last modified:January 1, 1988 - v1
Checksum:iB18E68BCE1DB330A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941T → K in AAA99516 (PubMed:2833614).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05015 Genomic DNA. Translation: CAA28666.1.
M20325 mRNA. Translation: AAA99516.1.
X04773 Genomic DNA. Translation: CAA28468.1.
A06324 Unassigned DNA. Translation: CAA00541.1.
M26798 Genomic DNA. Translation: AAA46948.1.
PIRiC26165. W1WL18.
RefSeqiNP_040312.1. NC_001357.1.

Genome annotation databases

GeneIDi1489084.
KEGGivg:1489084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05015 Genomic DNA. Translation: CAA28666.1.
M20325 mRNA. Translation: AAA99516.1.
X04773 Genomic DNA. Translation: CAA28468.1.
A06324 Unassigned DNA. Translation: CAA00541.1.
M26798 Genomic DNA. Translation: AAA46948.1.
PIRiC26165. W1WL18.
RefSeqiNP_040312.1. NC_001357.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R9WX-ray1.80A210-354[»]
1TUEX-ray2.10A/D/F/H/K/M428-631[»]
ProteinModelPortaliP06789.
SMRiP06789. Positions 210-354, 360-629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP06789. 2 interactions.
MINTiMINT-94927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489084.
KEGGivg:1489084.

Miscellaneous databases

EvolutionaryTraceiP06789.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04000. PPV_E1. 1 hit.
InterProiIPR001177. DNA_helicase_E1_C_Papillomavir.
IPR014000. DNA_helicase_E1_N_Papillomavir.
IPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR016393. Rep_E1_papillomaV.
[Graphical view]
PfamiPF00519. PPV_E1_C. 1 hit.
PF00524. PPV_E1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003383. Rep_E1_papillomaV. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51206. SF3_HELICASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVE1_HPV18
AccessioniPrimary (citable) accession number: P06789
Secondary accession number(s): Q84181
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.