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Protein

Replication protein E1

Gene

E1

Organism
Human papillomavirus type 18
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi484 – 491ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • ATP-dependent DNA helicase activity Source: BHF-UCL
  • DNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  • DNA replication Source: UniProtKB-HAMAP
  • viral genome replication Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein E1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase E1UniRule annotation
Gene namesi
Name:E1UniRule annotation
OrganismiHuman papillomavirus type 18
Taxonomic identifieri333761 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeAlphapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009109 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

GO - Cellular componenti

  • host cell cytoplasm Source: BHF-UCL
  • host cell nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi225 – 226SF → PS: Complete loss of SMARCB1/INI1 binding. 1 Publication2
Mutagenesisi305L → K: Complete loss of SMARCB1/INI1 binding. 1 Publication1
Mutagenesisi395 – 396NA → HE: Complete loss of SMARCB1/INI1 binding. 1 Publication2
Mutagenesisi418 – 419HY → EH: Complete loss of SMARCB1/INI1 binding. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001331161 – 657Replication protein E1Add BLAST657

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110Phosphoserine; by hostUniRule annotation1
Cross-linki565Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation

Post-translational modificationi

Phosphorylated.UniRule annotation
Sumoylated.UniRule annotation

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.Interacts with human SMARCB1/INI1 protein, stimulating viral replication.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
IFIT1P099148EBI-7015660,EBI-745117From a different organism.
SMARCB1Q12824-15EBI-7015660,EBI-7015645From a different organism.

Protein-protein interaction databases

IntActiP06789. 2 interactors.
MINTiMINT-94927.

Structurei

Secondary structure

1657
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi211 – 222Combined sources12
Helixi226 – 228Combined sources3
Beta strandi240 – 250Combined sources11
Helixi252 – 256Combined sources5
Helixi258 – 262Combined sources5
Helixi263 – 265Combined sources3
Beta strandi266 – 276Combined sources11
Beta strandi279 – 292Combined sources14
Helixi294 – 305Combined sources12
Helixi309 – 311Combined sources3
Beta strandi312 – 315Combined sources4
Helixi322 – 333Combined sources12
Beta strandi337 – 341Combined sources5
Helixi345 – 350Combined sources6
Helixi429 – 438Combined sources10
Helixi447 – 455Combined sources9
Helixi460 – 472Combined sources13
Beta strandi478 – 484Combined sources7
Helixi486 – 488Combined sources3
Helixi490 – 501Combined sources12
Beta strandi511 – 513Combined sources3
Helixi515 – 520Combined sources6
Beta strandi524 – 531Combined sources8
Helixi533 – 542Combined sources10
Helixi544 – 548Combined sources5
Beta strandi552 – 554Combined sources3
Beta strandi557 – 559Combined sources3
Beta strandi562 – 564Combined sources3
Beta strandi569 – 575Combined sources7
Beta strandi579 – 582Combined sources4
Helixi584 – 587Combined sources4
Beta strandi591 – 594Combined sources4
Helixi613 – 623Combined sources11
Helixi625 – 627Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R9WX-ray1.80A210-354[»]
1TUEX-ray2.10A/D/F/H/K/M428-631[»]
ProteinModelPortaliP06789.
SMRiP06789.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06789.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini458 – 608SF3 helicaseUniRule annotationAdd BLAST151

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 444SMARCB1/INI1-bindingAdd BLAST298
Regioni193 – 359DNA-binding regionUniRule annotationAdd BLAST167

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi86 – 88Nuclear localization signalUniRule annotation3
Motifi109 – 118Nuclear export signalUniRule annotation10

Sequence similaritiesi

Belongs to the papillomaviridae E1 protein family.UniRule annotation
Contains 1 SF3 helicase domain.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04000. PPV_E1. 1 hit.
InterProiIPR001177. DNA_helicase_E1_C_Papillomavir.
IPR014000. DNA_helicase_E1_N_Papillomavir.
IPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR016393. Rep_E1_papillomaV.
[Graphical view]
PfamiPF00519. PPV_E1_C. 1 hit.
PF00524. PPV_E1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003383. Rep_E1_papillomaV. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51206. SF3_HELICASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPEGTDGE GTGCNGWFYV QAIVDKKTGD VISDDEDENA TDTGSDMVDF
60 70 80 90 100
IDTQGTFCEQ AELETAQALF HAQEVHNDAQ VLHVLKRKFA GGSTENSPLG
110 120 130 140 150
ERLEVDTELS PRLQEISLNS GQKKAKRRLF TISDSGYGCS EVEATQIQVT
160 170 180 190 200
TNGEHGGNVC SGGSTEAIDN GGTEGNNSSV DGTSDNSNIE NVNPQCTIAQ
210 220 230 240 250
LKDLLKVNNK QGAMLAVFKD TYGLSFTDLV RNFKSDKTTC TDWVTAIFGV
260 270 280 290 300
NPTIAEGFKT LIQPFILYAH IQCLDCKWGV LILALLRYKC GKSRLTVAKG
310 320 330 340 350
LSTLLHVPET CMLIQPPKLR SSVAALYWYR TGISNISEVM GDTPEWIQRL
360 370 380 390 400
TIIQHGIDDS NFDLSEMVQW AFDNELTDES DMAFEYALLA DSNSNAAAFL
410 420 430 440 450
KSNCQAKYLK DCATMCKHYR RAQKRQMNMS QWIRFRCSKI DEGGDWRPIV
460 470 480 490 500
QFLRYQQIEF ITFLGALKSF LKGTPKKNCL VFCGPANTGK SYFGMSFIHF
510 520 530 540 550
IQGAVISFVN STSHFWLEPL TDTKVAMLDD ATTTCWTYFD TYMRNALDGN
560 570 580 590 600
PISIDRKHKP LIQLKCPPIL LTTNIHPAKD NRWPYLESRI TVFEFPNAFP
610 620 630 640 650
FDKNGNPVYE INDKNWKCFF ERTWSRLDLH EEEEDADTEG NPFGTFKLRA

GQNHRPL
Length:657
Mass (Da):73,736
Last modified:January 1, 1988 - v1
Checksum:iB18E68BCE1DB330A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94T → K in AAA99516 (PubMed:2833614).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05015 Genomic DNA. Translation: CAA28666.1.
M20325 mRNA. Translation: AAA99516.1.
X04773 Genomic DNA. Translation: CAA28468.1.
A06324 Unassigned DNA. Translation: CAA00541.1.
M26798 Genomic DNA. Translation: AAA46948.1.
PIRiC26165. W1WL18.
RefSeqiNP_040312.1. NC_001357.1.

Genome annotation databases

GeneIDi1489084.
KEGGivg:1489084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05015 Genomic DNA. Translation: CAA28666.1.
M20325 mRNA. Translation: AAA99516.1.
X04773 Genomic DNA. Translation: CAA28468.1.
A06324 Unassigned DNA. Translation: CAA00541.1.
M26798 Genomic DNA. Translation: AAA46948.1.
PIRiC26165. W1WL18.
RefSeqiNP_040312.1. NC_001357.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R9WX-ray1.80A210-354[»]
1TUEX-ray2.10A/D/F/H/K/M428-631[»]
ProteinModelPortaliP06789.
SMRiP06789.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP06789. 2 interactors.
MINTiMINT-94927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489084.
KEGGivg:1489084.

Miscellaneous databases

EvolutionaryTraceiP06789.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04000. PPV_E1. 1 hit.
InterProiIPR001177. DNA_helicase_E1_C_Papillomavir.
IPR014000. DNA_helicase_E1_N_Papillomavir.
IPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR016393. Rep_E1_papillomaV.
[Graphical view]
PfamiPF00519. PPV_E1_C. 1 hit.
PF00524. PPV_E1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003383. Rep_E1_papillomaV. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51206. SF3_HELICASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVE1_HPV18
AccessioniPrimary (citable) accession number: P06789
Secondary accession number(s): Q84181
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.