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Protein

Calmodulin

Gene

CMD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Component of the spindle pole body (SPB) required for the proper execution of spindle pole body (SPB) duplication.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi21 – 321Add BLAST12
Calcium bindingi57 – 682Add BLAST12
Calcium bindingi94 – 1053Add BLAST12

GO - Molecular functioni

  • calcium-dependent protein binding Source: SGD
  • calcium ion binding Source: SGD
  • mitogen-activated protein kinase binding Source: SGD

GO - Biological processi

  • cell budding Source: SGD
  • cytoskeleton organization Source: SGD
  • endocytosis Source: SGD
  • karyogamy involved in conjugation with cellular fusion Source: SGD
  • lysosomal microautophagy Source: SGD
  • NLS-bearing protein import into nucleus Source: SGD
  • phosphatidylinositol biosynthetic process Source: SGD
  • receptor-mediated endocytosis Source: SGD
  • regulation of membrane tubulation Source: SGD
  • spindle pole body organization Source: SGD
  • transcription factor import into nucleus Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29071-MONOMER.
ReactomeiR-SCE-111932. CaMK IV-mediated phosphorylation of CREB.
R-SCE-111933. Calmodulin induced events.
R-SCE-114608. Platelet degranulation.
R-SCE-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-SCE-203615. eNOS activation.
R-SCE-2871809. FCERI mediated Ca+2 mobilization.
R-SCE-4086398. Ca2+ pathway.
R-SCE-442729. CREB phosphorylation through the activation of CaMKII.
R-SCE-442745. Activation of CaMK IV.
R-SCE-445355. Smooth Muscle Contraction.
R-SCE-5218920. VEGFR2 mediated vascular permeability.
R-SCE-5578775. Ion homeostasis.
R-SCE-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-SCE-5626467. RHO GTPases activate IQGAPs.
R-SCE-5627123. RHO GTPases activate PAKs.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin
Short name:
CaM
Gene namesi
Name:CMD1
Ordered Locus Names:YBR109C
ORF Names:YBR0904
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR109C.
SGDiS000000313. CMD1.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • central plaque of spindle pole body Source: SGD
  • incipient cellular bud site Source: SGD
  • mating projection tip Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21D → A: Highly reduced affinity for Ca(2+). 1 Publication1
Mutagenesisi32E → V: Highly reduced affinity for Ca(2+). 1 Publication1
Mutagenesisi57D → A: Highly reduced affinity for Ca(2+). 1 Publication1
Mutagenesisi68E → V: Highly reduced affinity for Ca(2+). 1 Publication1
Mutagenesisi94D → A: Highly reduced affinity for Ca(2+). 1 Publication1
Mutagenesisi105E → V: Highly reduced affinity for Ca(2+). 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1667687.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983281 – 147CalmodulinAdd BLAST147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei82PhosphoserineCombined sources1
Modified residuei102PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06787.
PRIDEiP06787.
TopDownProteomicsiP06787.

PTM databases

iPTMnetiP06787.

Interactioni

Subunit structurei

Component of the SPC110 complex containing at least CMD1, SPC29, SPC42 and SCP110. Interacts with SPC110.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CNA1P232873EBI-3976,EBI-12771
MYO5Q044397EBI-3976,EBI-11687
SPC110P323802EBI-3976,EBI-12369

GO - Molecular functioni

  • calcium-dependent protein binding Source: SGD
  • mitogen-activated protein kinase binding Source: SGD

Protein-protein interaction databases

BioGridi32813. 196 interactors.
DIPiDIP-536N.
IntActiP06787. 34 interactors.
MINTiMINT-655049.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 19Combined sources13
Beta strandi25 – 29Combined sources5
Helixi30 – 40Combined sources11
Helixi46 – 54Combined sources9
Beta strandi58 – 60Combined sources3
Beta strandi63 – 65Combined sources3
Helixi66 – 73Combined sources8
Helixi82 – 93Combined sources12
Beta strandi95 – 98Combined sources4
Beta strandi100 – 102Combined sources3
Helixi103 – 113Combined sources11
Helixi119 – 129Combined sources11
Beta strandi132 – 137Combined sources6
Helixi138 – 145Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F54NMR-A2-78[»]
1F55NMR-A2-78[»]
1LKJNMR-A2-147[»]
2LHHNMR-A2-121[»]
2LHINMR-A2-147[»]
ProteinModelPortaliP06787.
SMRiP06787.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP06787.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 43EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini44 – 79EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 116EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini117 – 147EF-hand 4PROSITE-ProRule annotationAdd BLAST31

Sequence similaritiesi

Belongs to the calmodulin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
InParanoidiP06787.
KOiK02183.
OMAiDEMSEAF.
OrthoDBiEOG092C4XFN.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06787-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNLTEEQI AEFKEAFALF DKDNNGSISS SELATVMRSL GLSPSEAEVN
60 70 80 90 100
DLMNEIDVDG NHQIEFSEFL ALMSRQLKSN DSEQELLEAF KVFDKNGDGL
110 120 130 140
ISAAELKHVL TSIGEKLTDA EVDDMLREVS DGSGEINIQQ FAALLSK
Length:147
Mass (Da):16,135
Last modified:January 1, 1988 - v1
Checksum:i819ED1AD5D9400D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14760 Genomic DNA. Translation: AAA34504.1.
AF081667 Genomic DNA. Translation: AAC68888.1.
X78993 Genomic DNA. Translation: CAA55612.1.
Z35978 Genomic DNA. Translation: CAA85064.1.
AY558184 Genomic DNA. Translation: AAS56510.1.
BK006936 Genomic DNA. Translation: DAA07228.1.
PIRiA25060. MCBY.
RefSeqiNP_009667.1. NM_001178457.1.

Genome annotation databases

EnsemblFungiiYBR109C; YBR109C; YBR109C.
GeneIDi852406.
KEGGisce:YBR109C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14760 Genomic DNA. Translation: AAA34504.1.
AF081667 Genomic DNA. Translation: AAC68888.1.
X78993 Genomic DNA. Translation: CAA55612.1.
Z35978 Genomic DNA. Translation: CAA85064.1.
AY558184 Genomic DNA. Translation: AAS56510.1.
BK006936 Genomic DNA. Translation: DAA07228.1.
PIRiA25060. MCBY.
RefSeqiNP_009667.1. NM_001178457.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F54NMR-A2-78[»]
1F55NMR-A2-78[»]
1LKJNMR-A2-147[»]
2LHHNMR-A2-121[»]
2LHINMR-A2-147[»]
ProteinModelPortaliP06787.
SMRiP06787.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32813. 196 interactors.
DIPiDIP-536N.
IntActiP06787. 34 interactors.
MINTiMINT-655049.

Chemistry databases

ChEMBLiCHEMBL1667687.

PTM databases

iPTMnetiP06787.

Proteomic databases

MaxQBiP06787.
PRIDEiP06787.
TopDownProteomicsiP06787.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR109C; YBR109C; YBR109C.
GeneIDi852406.
KEGGisce:YBR109C.

Organism-specific databases

EuPathDBiFungiDB:YBR109C.
SGDiS000000313. CMD1.

Phylogenomic databases

GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
InParanoidiP06787.
KOiK02183.
OMAiDEMSEAF.
OrthoDBiEOG092C4XFN.

Enzyme and pathway databases

BioCyciYEAST:G3O-29071-MONOMER.
ReactomeiR-SCE-111932. CaMK IV-mediated phosphorylation of CREB.
R-SCE-111933. Calmodulin induced events.
R-SCE-114608. Platelet degranulation.
R-SCE-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-SCE-203615. eNOS activation.
R-SCE-2871809. FCERI mediated Ca+2 mobilization.
R-SCE-4086398. Ca2+ pathway.
R-SCE-442729. CREB phosphorylation through the activation of CaMKII.
R-SCE-442745. Activation of CaMK IV.
R-SCE-445355. Smooth Muscle Contraction.
R-SCE-5218920. VEGFR2 mediated vascular permeability.
R-SCE-5578775. Ion homeostasis.
R-SCE-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-SCE-5626467. RHO GTPases activate IQGAPs.
R-SCE-5627123. RHO GTPases activate PAKs.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-936837. Ion transport by P-type ATPases.

Miscellaneous databases

EvolutionaryTraceiP06787.
PROiP06787.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALM_YEAST
AccessioniPrimary (citable) accession number: P06787
Secondary accession number(s): D6VQA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 184 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein has three functional calcium-binding sites.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.