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P06785 (TYSY_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Alternative name(s):
Cell division cycle protein 21
Constitutive RNR3 transcription protein 9
Gene names
Name:CDC21
Synonyms:CRT9, TMP1
Ordered Locus Names:YOR074C
ORF Names:YOR29-25
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thymidylate synthase required for de novo biosynthesis of pyrimidine deoxyribonucleotides. Required for both nuclear and mitochondrial DNA synthesis. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. Ref.6

Enzyme regulation

Inhibited by 5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP). Ref.10

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer. Ref.6

Subcellular location

Nucleus. Note: Localizes to the nuclear periphery. Ref.16

Induction

Transcribed in a periodic manner during the cell cycle with maximal mRNA levels occurring just prior to the onset of DNA replication. The promoter contains 8-base-pair motifs (ACGCGTNA) called the MluI cell-cycle boxes (MCBs), which mediate transcription regulation by SWI6. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15

Sequence similarities

Belongs to the thymidylate synthase family.

Biophysicochemical properties

Kinetic parameters:

KM=5 µM for dUMP Ref.6

KM=70 µM for 5,10-methylene-tetrahydropteroylglutamate

pH dependence:

Optimum pH is 6.8-7.2.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentNucleus
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from mutant phenotype Ref.9PubMed 794696. Source: SGD

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentnucleus

Inferred from direct assay Ref.16. Source: SGD

   Molecular_functionthymidylate synthase activity

Inferred from direct assay Ref.10. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140912

Sites

Active site1771 By similarity

Experimental info

Sequence conflict34 – 7845Missing in CAA99267. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P06785 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 0C514BEDB8574510

FASTA30435,047
        10         20         30         40         50         60 
MTMDGKNKEE EQYLDLCKRI IDEGEFRPDR TGTGTLSLFA PPQLRFSLRD DTFPLLTTKK 

        70         80         90        100        110        120 
VFTRGIILEL LWFLAGDTDA NLLSEQGVKI WDGNGSREYL DKMGFKDRKV GDLGPVYGFQ 

       130        140        150        160        170        180 
WRHFGAKYKT CDDDYTGQGI DQLKQVIHKL KTNPYDRRII MSAWNPADFD KMALPPCHIF 

       190        200        210        220        230        240 
SQFYVSFPKE GEGSGKPRLS CLLYQRSCDM GLGVPFNIAS YALLTRMIAK VVDMEPGEFI 

       250        260        270        280        290        300 
HTLGDAHVYK DHIDALKEQI TRNPRPFPKL KIKRDVKDID DFKLTDFEIE DYNPHPRIQM 


KMSV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of the cell cycle-regulated thymidylate synthase gene of Saccharomyces cerevisiae."
Taylor G.R., Lagosky P.A., Storms R.K., Haynes R.H.
J. Biol. Chem. 262:5298-5307(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
Yeast 13:379-390(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Transcriptional regulation of the cell cycle-dependent thymidylate synthase gene of Saccharomyces cerevisiae."
McIntosh E.M., Ord R.W., Storms R.K.
Mol. Cell. Biol. 8:4616-4624(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
[6]"Thymidylate synthetase from Saccharomyces cerevisiae. Purification and enzymic properties."
Bisson L.F., Thorner J.
J. Biol. Chem. 256:12456-12462(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-18, SUBUNIT, CATALYTIC ACTIVITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[7]"Genetic control of the cell division cycle in yeast: V. Genetic analysis of cdc mutants."
Hartwell L.H., Mortimer R.K., Culotti J., Culotti M.
Genetics 74:267-286(1973) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae."
Hartwell L.H.
J. Bacteriol. 115:966-974(1973) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Thymidine 5'-monophosphate-requiring mutants of Saccharomyces cerevisiae are deficient in thymidylate synthetase."
Bisson L., Thorner J.
J. Bacteriol. 132:44-50(1977) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Isolation of the thymidylate synthetase gene (TMP1) by complementation in Saccharomyces cerevisiae."
Taylor G.R., Barclay B.J., Storms R.K., Friesen J.D., Haynes R.H.
Mol. Cell. Biol. 2:437-442(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION.
[11]"The expression in meiosis of genes which are transcribed periodically in the mitotic cell cycle of budding yeast."
Johnston L.H., Johnson A.L., Barker D.G.
Exp. Cell Res. 165:541-549(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[12]"Transcription of genes encoding enzymes involved in DNA synthesis during the cell cycle of Saccharomyces cerevisiae."
McIntosh E.M., Gadsden M.H., Haynes R.H.
Mol. Gen. Genet. 204:363-366(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[13]"SWI6 is a regulatory subunit of two different cell cycle START-dependent transcription factors in Saccharomyces cerevisiae."
Moll T., Dirick L., Auer H., Bonkovsky J., Nasmyth K.
J. Cell Sci. Suppl. 16:87-96(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[14]"A central role for SWI6 in modulating cell cycle Start-specific transcription in yeast."
Dirick L., Moll T., Auer H., Nasmyth K.
Nature 357:508-513(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[15]"Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6."
Verma R., Smiley J., Andrews B., Campbell J.L.
Proc. Natl. Acad. Sci. U.S.A. 89:9479-9483(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[16]"Thymidylate synthase is localized to the nuclear periphery in the yeast Saccharomyces cerevisiae."
Poon P.P., Storms R.K.
J. Biol. Chem. 269:8341-8347(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02706 Genomic DNA. Translation: AAA60940.1.
Z70678 Genomic DNA. Translation: CAA94559.1.
Z74982 Genomic DNA. Translation: CAA99267.1.
M29100 Genomic DNA. Translation: AAA35159.1.
BK006948 Genomic DNA. Translation: DAA10853.1.
PIRYXBYT. S66957.
RefSeqNP_014717.2. NM_001183493.1.

3D structure databases

ProteinModelPortalP06785.
SMRP06785. Positions 7-304.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34473. 14 interactions.
DIPDIP-2786N.
IntActP06785. 2 interactions.
MINTMINT-562757.
STRING4932.YOR074C.

Proteomic databases

MaxQBP06785.
PaxDbP06785.
PeptideAtlasP06785.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR074C; YOR074C; YOR074C.
GeneID854241.
KEGGsce:YOR074C.

Organism-specific databases

CYGDYOR074c.
SGDS000005600. CDC21.

Phylogenomic databases

eggNOGCOG0207.
GeneTreeENSGT00390000014786.
HOGENOMHOG000257899.
KOK00560.
OMADVHLYSN.
OrthoDBEOG7X6M9G.

Enzyme and pathway databases

BioCycYEAST:YOR074C-MONOMER.
UniPathwayUPA00575.

Gene expression databases

GenevestigatorP06785.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976141.
PROP06785.

Entry information

Entry nameTYSY_YEAST
AccessionPrimary (citable) accession number: P06785
Secondary accession number(s): D6W2D7, Q12694
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: May 14, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways