Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidylate synthase

Gene

CDC21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thymidylate synthase required for de novo biosynthesis of pyrimidine deoxyribonucleotides. Required for both nuclear and mitochondrial DNA synthesis.5 Publications

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.1 Publication

Enzyme regulationi

Inhibited by 5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP).1 Publication

Kineticsi

  1. KM=5 µM for dUMP1 Publication
  2. KM=70 µM for 5,10-methylene-tetrahydropteroylglutamate1 Publication

    pH dependencei

    Optimum pH is 6.8-7.2.1 Publication

    Pathwayi: dTTP biosynthesis

    This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
    View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei30dUMPBy similarity1
    Active sitei177NucleophileBy similarity1
    Binding sitei2095,10-methylenetetrahydrofolateBy similarity1
    Binding sitei217dUMPBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi157 – 158dUMP; shared with dimeric partnerBy similarity2
    Nucleotide bindingi206 – 209dUMPBy similarity4
    Nucleotide bindingi247 – 249dUMPBy similarity3

    GO - Molecular functioni

    • thymidylate synthase activity Source: SGD

    GO - Biological processi

    • dTMP biosynthetic process Source: SGD
    • dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywordsi

    Molecular functionMethyltransferase, Transferase
    Biological processNucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YOR074C-MONOMER
    ReactomeiR-SCE-499943 Interconversion of nucleotide di- and triphosphates
    UniPathwayiUPA00575

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthase (EC:2.1.1.45)
    Short name:
    TS
    Short name:
    TSase
    Alternative name(s):
    Cell division cycle protein 21
    Constitutive RNR3 transcription protein 9
    Gene namesi
    Name:CDC21
    Synonyms:CRT9, TMP1
    Ordered Locus Names:YOR074C
    ORF Names:YOR29-25
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOR074C
    SGDiS000005600 CDC21

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001409121 – 304Thymidylate synthaseAdd BLAST304

    Proteomic databases

    MaxQBiP06785
    PaxDbiP06785
    PRIDEiP06785

    Expressioni

    Inductioni

    Transcribed in a periodic manner during the cell cycle with maximal mRNA levels occurring just prior to the onset of DNA replication. The promoter contains 8-base-pair motifs (ACGCGTNA) called the MluI cell-cycle boxes (MCBs), which mediate transcription regulation by SWI6.5 Publications

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi34473, 386 interactors
    DIPiDIP-2786N
    IntActiP06785, 2 interactors
    MINTiP06785
    STRINGi4932.YOR074C

    Structurei

    3D structure databases

    ProteinModelPortaliP06785
    SMRiP06785
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000014786
    HOGENOMiHOG000257899
    InParanoidiP06785
    KOiK00560
    OMAiKQYLDLC
    OrthoDBiEOG092C3QQ6

    Family and domain databases

    CDDicd00351 TS_Pyrimidine_HMase, 1 hit
    Gene3Di3.30.572.10, 1 hit
    HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
    InterProiView protein in InterPro
    IPR023451 Thymidate_synth/dCMP_Mease
    IPR036926 Thymidate_synth/dCMP_Mease_sf
    IPR000398 Thymidylate_synthase
    IPR020940 Thymidylate_synthase_AS
    PfamiView protein in Pfam
    PF00303 Thymidylat_synt, 1 hit
    PRINTSiPR00108 THYMDSNTHASE
    SUPFAMiSSF55831 SSF55831, 1 hit
    TIGRFAMsiTIGR03284 thym_sym, 1 hit
    PROSITEiView protein in PROSITE
    PS00091 THYMIDYLATE_SYNTHASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P06785-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTMDGKNKEE EQYLDLCKRI IDEGEFRPDR TGTGTLSLFA PPQLRFSLRD
    60 70 80 90 100
    DTFPLLTTKK VFTRGIILEL LWFLAGDTDA NLLSEQGVKI WDGNGSREYL
    110 120 130 140 150
    DKMGFKDRKV GDLGPVYGFQ WRHFGAKYKT CDDDYTGQGI DQLKQVIHKL
    160 170 180 190 200
    KTNPYDRRII MSAWNPADFD KMALPPCHIF SQFYVSFPKE GEGSGKPRLS
    210 220 230 240 250
    CLLYQRSCDM GLGVPFNIAS YALLTRMIAK VVDMEPGEFI HTLGDAHVYK
    260 270 280 290 300
    DHIDALKEQI TRNPRPFPKL KIKRDVKDID DFKLTDFEIE DYNPHPRIQM

    KMSV
    Length:304
    Mass (Da):35,047
    Last modified:January 1, 1988 - v1
    Checksum:i0C514BEDB8574510
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti34 – 78Missing in CAA99267 (PubMed:9169874).CuratedAdd BLAST45

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J02706 Genomic DNA Translation: AAA60940.1
    Z70678 Genomic DNA Translation: CAA94559.1
    Z74982 Genomic DNA Translation: CAA99267.1
    M29100 Genomic DNA Translation: AAA35159.1
    BK006948 Genomic DNA Translation: DAA10853.1
    PIRiS66957 YXBYT
    RefSeqiNP_014717.2, NM_001183493.1

    Genome annotation databases

    EnsemblFungiiYOR074C; YOR074C; YOR074C
    GeneIDi854241
    KEGGisce:YOR074C

    Similar proteinsi

    Entry informationi

    Entry nameiTYSY_YEAST
    AccessioniPrimary (citable) accession number: P06785
    Secondary accession number(s): D6W2D7, Q12694
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: May 23, 2018
    This is version 155 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health