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Protein

Thymidylate synthase

Gene

CDC21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thymidylate synthase required for de novo biosynthesis of pyrimidine deoxyribonucleotides. Required for both nuclear and mitochondrial DNA synthesis.5 Publications

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.1 Publication

Enzyme regulationi

Inhibited by 5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP).1 Publication

Kineticsi

  1. KM=5 µM for dUMP1 Publication
  2. KM=70 µM for 5,10-methylene-tetrahydropteroylglutamate1 Publication

pH dependencei

Optimum pH is 6.8-7.2.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771By similarity

GO - Molecular functioni

  1. thymidylate synthase activity Source: SGD

GO - Biological processi

  1. dTMP biosynthetic process Source: SGD
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YOR074C-MONOMER.
ReactomeiREACT_303865. E2F mediated regulation of DNA replication.
REACT_320581. Pyrimidine biosynthesis.
REACT_345909. G1/S-Specific Transcription.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Alternative name(s):
Cell division cycle protein 21
Constitutive RNR3 transcription protein 9
Gene namesi
Name:CDC21
Synonyms:CRT9, TMP1
Ordered Locus Names:YOR074C
ORF Names:YOR29-25
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOR074c.
SGDiS000005600. CDC21.

Subcellular locationi

Nucleus 1 Publication
Note: Localizes to the nuclear periphery.

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Thymidylate synthasePRO_0000140912Add
BLAST

Proteomic databases

MaxQBiP06785.
PaxDbiP06785.
PeptideAtlasiP06785.

Expressioni

Inductioni

Transcribed in a periodic manner during the cell cycle with maximal mRNA levels occurring just prior to the onset of DNA replication. The promoter contains 8-base-pair motifs (ACGCGTNA) called the MluI cell-cycle boxes (MCBs), which mediate transcription regulation by SWI6.5 Publications

Gene expression databases

GenevestigatoriP06785.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi34473. 16 interactions.
DIPiDIP-2786N.
IntActiP06785. 2 interactions.
MINTiMINT-562757.
STRINGi4932.YOR074C.

Structurei

3D structure databases

ProteinModelPortaliP06785.
SMRiP06785. Positions 7-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0207.
GeneTreeiENSGT00390000014786.
HOGENOMiHOG000257899.
InParanoidiP06785.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG7X6M9G.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMDGKNKEE EQYLDLCKRI IDEGEFRPDR TGTGTLSLFA PPQLRFSLRD
60 70 80 90 100
DTFPLLTTKK VFTRGIILEL LWFLAGDTDA NLLSEQGVKI WDGNGSREYL
110 120 130 140 150
DKMGFKDRKV GDLGPVYGFQ WRHFGAKYKT CDDDYTGQGI DQLKQVIHKL
160 170 180 190 200
KTNPYDRRII MSAWNPADFD KMALPPCHIF SQFYVSFPKE GEGSGKPRLS
210 220 230 240 250
CLLYQRSCDM GLGVPFNIAS YALLTRMIAK VVDMEPGEFI HTLGDAHVYK
260 270 280 290 300
DHIDALKEQI TRNPRPFPKL KIKRDVKDID DFKLTDFEIE DYNPHPRIQM

KMSV
Length:304
Mass (Da):35,047
Last modified:December 31, 1987 - v1
Checksum:i0C514BEDB8574510
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 7845Missing in CAA99267 (PubMed:9169874).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02706 Genomic DNA. Translation: AAA60940.1.
Z70678 Genomic DNA. Translation: CAA94559.1.
Z74982 Genomic DNA. Translation: CAA99267.1.
M29100 Genomic DNA. Translation: AAA35159.1.
BK006948 Genomic DNA. Translation: DAA10853.1.
PIRiS66957. YXBYT.
RefSeqiNP_014717.2. NM_001183493.1.

Genome annotation databases

EnsemblFungiiYOR074C; YOR074C; YOR074C.
GeneIDi854241.
KEGGisce:YOR074C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02706 Genomic DNA. Translation: AAA60940.1.
Z70678 Genomic DNA. Translation: CAA94559.1.
Z74982 Genomic DNA. Translation: CAA99267.1.
M29100 Genomic DNA. Translation: AAA35159.1.
BK006948 Genomic DNA. Translation: DAA10853.1.
PIRiS66957. YXBYT.
RefSeqiNP_014717.2. NM_001183493.1.

3D structure databases

ProteinModelPortaliP06785.
SMRiP06785. Positions 7-304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34473. 16 interactions.
DIPiDIP-2786N.
IntActiP06785. 2 interactions.
MINTiMINT-562757.
STRINGi4932.YOR074C.

Proteomic databases

MaxQBiP06785.
PaxDbiP06785.
PeptideAtlasiP06785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR074C; YOR074C; YOR074C.
GeneIDi854241.
KEGGisce:YOR074C.

Organism-specific databases

CYGDiYOR074c.
SGDiS000005600. CDC21.

Phylogenomic databases

eggNOGiCOG0207.
GeneTreeiENSGT00390000014786.
HOGENOMiHOG000257899.
InParanoidiP06785.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG7X6M9G.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciYEAST:YOR074C-MONOMER.
ReactomeiREACT_303865. E2F mediated regulation of DNA replication.
REACT_320581. Pyrimidine biosynthesis.
REACT_345909. G1/S-Specific Transcription.

Miscellaneous databases

NextBioi976141.
PROiP06785.

Gene expression databases

GenevestigatoriP06785.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the cell cycle-regulated thymidylate synthase gene of Saccharomyces cerevisiae."
    Taylor G.R., Lagosky P.A., Storms R.K., Haynes R.H.
    J. Biol. Chem. 262:5298-5307(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
    Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
    Yeast 13:379-390(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Transcriptional regulation of the cell cycle-dependent thymidylate synthase gene of Saccharomyces cerevisiae."
    McIntosh E.M., Ord R.W., Storms R.K.
    Mol. Cell. Biol. 8:4616-4624(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
  6. "Thymidylate synthetase from Saccharomyces cerevisiae. Purification and enzymic properties."
    Bisson L.F., Thorner J.
    J. Biol. Chem. 256:12456-12462(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-18, SUBUNIT, CATALYTIC ACTIVITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  7. "Genetic control of the cell division cycle in yeast: V. Genetic analysis of cdc mutants."
    Hartwell L.H., Mortimer R.K., Culotti J., Culotti M.
    Genetics 74:267-286(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae."
    Hartwell L.H.
    J. Bacteriol. 115:966-974(1972) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Thymidine 5'-monophosphate-requiring mutants of Saccharomyces cerevisiae are deficient in thymidylate synthetase."
    Bisson L., Thorner J.
    J. Bacteriol. 132:44-50(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Isolation of the thymidylate synthetase gene (TMP1) by complementation in Saccharomyces cerevisiae."
    Taylor G.R., Barclay B.J., Storms R.K., Friesen J.D., Haynes R.H.
    Mol. Cell. Biol. 2:437-442(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  11. "The expression in meiosis of genes which are transcribed periodically in the mitotic cell cycle of budding yeast."
    Johnston L.H., Johnson A.L., Barker D.G.
    Exp. Cell Res. 165:541-549(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  12. "Transcription of genes encoding enzymes involved in DNA synthesis during the cell cycle of Saccharomyces cerevisiae."
    McIntosh E.M., Gadsden M.H., Haynes R.H.
    Mol. Gen. Genet. 204:363-366(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  13. "SWI6 is a regulatory subunit of two different cell cycle START-dependent transcription factors in Saccharomyces cerevisiae."
    Moll T., Dirick L., Auer H., Bonkovsky J., Nasmyth K.
    J. Cell Sci. Suppl. 16:87-96(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  14. "A central role for SWI6 in modulating cell cycle Start-specific transcription in yeast."
    Dirick L., Moll T., Auer H., Nasmyth K.
    Nature 357:508-513(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  15. "Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6."
    Verma R., Smiley J., Andrews B., Campbell J.L.
    Proc. Natl. Acad. Sci. U.S.A. 89:9479-9483(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  16. "Thymidylate synthase is localized to the nuclear periphery in the yeast Saccharomyces cerevisiae."
    Poon P.P., Storms R.K.
    J. Biol. Chem. 269:8341-8347(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTYSY_YEAST
AccessioniPrimary (citable) accession number: P06785
Secondary accession number(s): D6W2D7, Q12694
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 31, 1987
Last sequence update: December 31, 1987
Last modified: March 31, 2015
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.