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P06784

- STE7_YEAST

UniProt

P06784 - STE7_YEAST

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Protein
Serine/threonine-protein kinase STE7
Gene
STE7, YDL159W, D1525
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase required for cell-type-specific transcription and signal transduction in yeast. It is thought that it is phosphorylated by the ste11 protein kinase and that it can phosphorylate the FUS3 and or KSS1 kinases.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Phosphorylated at multiple sites in response to pheromone.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATP By similarity
Active sitei331 – 3311Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi197 – 2059ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase kinase activity Source: SGD
  3. protein binding Source: IntAct
  4. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. MAPK cascade involved in cell wall organization or biogenesis Source: SGD
  2. MAPK cascade involved in conjugation with cellular fusion Source: SGD
  3. activation of MAPK activity involved in conjugation with cellular fusion Source: SGD
  4. invasive growth in response to glucose limitation Source: SGD
  5. protein phosphorylation Source: SGD
  6. pseudohyphal growth Source: SGD
  7. regulation of transposition, RNA-mediated Source: SGD
  8. signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Pheromone response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29553-MONOMER.
BRENDAi2.7.12.2. 984.
ReactomeiREACT_191508. Signaling by FGFR.
REACT_207653. Signal transduction by L1.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase STE7 (EC:2.7.12.2)
Gene namesi
Name:STE7
Ordered Locus Names:YDL159W
ORF Names:D1525
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL159w.
SGDiS000002318. STE7.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. mating projection tip Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi353 – 3531S → A: No loss of activity.
Mutagenesisi359 – 3591S → A: Inactivation.
Mutagenesisi363 – 3631T → A: Inactivation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Serine/threonine-protein kinase STE7
PRO_0000086688Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei359 – 3591Phosphoserine1 Publication
Modified residuei363 – 3631Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06784.
PaxDbiP06784.
PeptideAtlasiP06784.

Expressioni

Gene expression databases

GenevestigatoriP06784.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-18389,EBI-4192
FUS3P1689215EBI-18389,EBI-7193
HOG1P324852EBI-18389,EBI-8437
KSS1P1468114EBI-18389,EBI-9945
STE5P329178EBI-18389,EBI-18373

Protein-protein interaction databases

BioGridi31905. 44 interactions.
DIPiDIP-9N.
IntActiP06784. 27 interactions.
MINTiMINT-411435.
STRINGi4932.YDL159W.

Structurei

3D structure databases

ProteinModelPortaliP06784.
SMRiP06784. Positions 190-494.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini191 – 466276Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00690000102311.
HOGENOMiHOG000234206.
KOiK11226.
OMAiEIIILME.
OrthoDBiEOG7380F1.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06784-1 [UniParc]FASTAAdd to Basket

« Hide

MFQRKTLQRR NLKGLNLNLH PDVGNNGQLQ EKTETHQGQS RIEGHVMSNI    50
NAIQNNSNLF LRRGIKKKLT LDAFGDDQAI SKPNTVVIQQ PQNEPVLVLS 100
SLSQSPCVSS SSSLSTPCII DAYSNNFGLS PSSTNSTPST IQGLSNIATP 150
VENEHSISLP PLEESLSPAA ADLKDTLSGT SNGNYIQLQD LVQLGKIGAG 200
NSGTVVKALH VPDSKIVAKK TIPVEQNNST IINQLVRELS IVKNVKPHEN 250
IITFYGAYYN QHINNEIIIL MEYSDCGSLD KILSVYKRFV QRGTVSSKKT 300
WFNELTISKI AYGVLNGLDH LYRQYKIIHR DIKPSNVLIN SKGQIKLCDF 350
GVSKKLINSI ADTFVGTSTY MSPERIQGNV YSIKGDVWSL GLMIIELVTG 400
EFPLGGHNDT PDGILDLLQR IVNEPSPRLP KDRIYSKEMT DFVNRCCIKN 450
ERERSSIHEL LHHDLIMKYV SPSKDDKFRH WCRKIKSKIK EDKRIKREAL 500
DRAKLEKKQS ERSTH 515
Length:515
Mass (Da):57,709
Last modified:January 1, 1988 - v1
Checksum:iC8A75899CFBE8BDE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14097 Genomic DNA. Translation: AAA35118.1.
Z67750 Genomic DNA. Translation: CAA91587.1.
Z74207 Genomic DNA. Translation: CAA98732.1.
X97751 Genomic DNA. Translation: CAA66332.1.
BK006938 Genomic DNA. Translation: DAA11702.1.
PIRiA25048.
RefSeqiNP_010122.1. NM_001180219.1.

Genome annotation databases

EnsemblFungiiYDL159W; YDL159W; YDL159W.
GeneIDi851396.
KEGGisce:YDL159W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14097 Genomic DNA. Translation: AAA35118.1 .
Z67750 Genomic DNA. Translation: CAA91587.1 .
Z74207 Genomic DNA. Translation: CAA98732.1 .
X97751 Genomic DNA. Translation: CAA66332.1 .
BK006938 Genomic DNA. Translation: DAA11702.1 .
PIRi A25048.
RefSeqi NP_010122.1. NM_001180219.1.

3D structure databases

ProteinModelPortali P06784.
SMRi P06784. Positions 190-494.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31905. 44 interactions.
DIPi DIP-9N.
IntActi P06784. 27 interactions.
MINTi MINT-411435.
STRINGi 4932.YDL159W.

Proteomic databases

MaxQBi P06784.
PaxDbi P06784.
PeptideAtlasi P06784.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL159W ; YDL159W ; YDL159W .
GeneIDi 851396.
KEGGi sce:YDL159W.

Organism-specific databases

CYGDi YDL159w.
SGDi S000002318. STE7.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00690000102311.
HOGENOMi HOG000234206.
KOi K11226.
OMAi EIIILME.
OrthoDBi EOG7380F1.

Enzyme and pathway databases

BioCyci YEAST:G3O-29553-MONOMER.
BRENDAi 2.7.12.2. 984.
Reactomei REACT_191508. Signaling by FGFR.
REACT_207653. Signal transduction by L1.

Miscellaneous databases

NextBioi 968558.
PROi P06784.

Gene expression databases

Genevestigatori P06784.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases."
    Teague M.A., Chaleff D.T., Errede B.
    Proc. Natl. Acad. Sci. U.S.A. 83:7371-7375(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Analysis of a 23 kb region on the left arm of yeast chromosome IV."
    Delaveau T.T.D., Blugeon C., Jacq C., Perea J.
    Yeast 12:1587-1592(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-515.
    Strain: ATCC 96604 / S288c / FY1679.
  5. "Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase."
    Cairns B.R., Ramer S.W., Kornberg K.D.
    Genes Dev. 6:1305-1318(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION.
  6. "Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues."
    Zheng C.-F., Guan K.-L.
    EMBO J. 13:1123-1131(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-359 AND THR-363, MUTAGENESIS.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSTE7_YEAST
AccessioniPrimary (citable) accession number: P06784
Secondary accession number(s): D6VRJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 3, 2014
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi