Reviewed, UniProtKB/Swiss-Prot P06783 (STE3_YEAST)

Names and origin

Protein names

Recommended name:
    Pheromone a factor receptor

Gene names

Name:	STE3
Ordered Locus Names:	YKL178C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for the peptide pheromone a factor.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 4 family.

Ontologies

Keywords
Biological process	Pheromone response
Cellular component	Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytogamy Inferred from direct assay. Source: SGD pheromone-dependent signal transduction involved in conjugation with cellular fusion Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to plasma membrane Ref.1 Inferred from direct assay. Source: SGD
Molecular function	mating-type a-factor pheromone receptor activity Ref.1 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Pheromone a factor receptor		PRO_0000195079
Regions
	Topological domain	1 – 5	5	Extracellular Potential
	Transmembrane	6 – 23	18	Potential
	Topological domain	24 – 29	6	Cytoplasmic Potential
	Transmembrane	30 – 53	24	Potential
	Topological domain	54 – 70	17	Extracellular Potential
	Transmembrane	71 – 98	28	Potential
	Topological domain	99 – 116	18	Cytoplasmic Potential
	Transmembrane	117 – 134	18	Potential
	Topological domain	135 – 155	21	Extracellular Potential
	Transmembrane	156 – 183	28	Potential
	Topological domain	184 – 205	22	Cytoplasmic Potential
	Transmembrane	206 – 228	23	Potential
	Topological domain	229 – 266	38	Extracellular Potential
	Transmembrane	267 – 285	19	Potential
	Topological domain	286 – 470	185	Cytoplasmic Potential
	Region	300 – 470	171	Hydrophilic
Amino acid modifications
	Modified residue	430	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P06783-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 5DBF611076B8CA91	FASTA	470	53,708
	10 20 30 40 50 60 MSYKSAIIGL CLLAVILLAP PLAWHSHTKN IPAIILITWL LTMNLTCIVD AAIWSDDDFL 70 80 90 100 110 120 TRWDGKGWCD IVIKLQVGAN IGISCAVTNI IYNLHTILKA DSVLPDLSSW TKIVKDLVIS 130 140 150 160 170 180 LFTPVMVMGF SYLLQVFRYG IARYNGCQNL LSPTWITTVL YTMWMLIWSF VGAVYATLVL 190 200 210 220 230 240 FVFYKKRKDV RDILHCTNSG LNLTRFARLL IFCFIIILVM FPFSVYTFVQ DLQQVEGHYT 250 260 270 280 290 300 FKNTHSSTIW NTIIKFDPGR PIYNIWLYVL MSYLVFLIFG LGSDALHMYS KFLRSIKLGF 310 320 330 340 350 360 VLDMWKRFID KNKEKRVGIL LNKLSSRKES RNPFSTDSEN YISTCTENYS PCVGTPISQA 370 380 390 400 410 420 HFYVDYRIPD DPRKSQNKSK KYLFADKETD DILDEIDLKE SRHIPYVTQG QSFDDEISLG 430 440 450 460 470 GFSKVTLDYS EKLHNSASSN FEGESLCYSP ASKEENSSSN EHSSENTAGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone a factor: gene sequence and implications for the structure of the presumed receptor." Hagen D.C., McCaffrey G., Sprague G.F. Jr. Proc. Natl. Acad. Sci. U.S.A. 83:1418-1422(1986) [PubMed: 3006051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes from Saccharomyces cerevisiae." Nakayama N., Miyajima A., Arai K. EMBO J. 4:2643-2648(1985) [PubMed: 16453635] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed: 8154185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-430, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M12239 Genomic DNA. Translation: AAA35113.1. X03011 Genomic DNA. Translation: CAA26795.1. X74151 Genomic DNA. Translation: CAA52261.1. Z28177 Genomic DNA. Translation: CAA82019.1. AY692742 Genomic DNA. Translation: AAT92761.1.
PIR	B24670.
RefSeq	NP_012743.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1146N.
IntAct	P06783. 2 interactions.
STRING	P06783.
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	YKL178C; YKL178C; YKL178C; Saccharomyces cerevisiae. [Genome view]
GeneID	853677.
KEGG	sce:YKL178C.
NMPDR	fig|4932.3.peg.3721.
Organism-specific databases
CYGD	YKL178c.
SGD	S000001661. STE3.
Phylogenomic databases
HOGENOM	P06783.
OMA	MSYLVFL
OrthoDB	EOG9M937H
Gene expression databases
ArrayExpress	P06783.
Genevestigator	P06783.
GermOnline	YKL178C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001499. STE3_GPCR. [Graphical view]
Pfam	PF02076. STE3. 1 hit. [Graphical view]
PRINTS	PR00899. GPCRSTE3.
ProtoNet	Search...
Other Resources
NextBio	974631.

Entry information

Entry name

STE3_YEAST

Accession

Primary (citable) accession number: P06783

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	November 24, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)