P06783 (STE3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pheromone a factor receptor

Gene names

Name:	STE3
Ordered Locus Names:	YKL178C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for the peptide pheromone a factor.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 4 family.

Ontologies

Keywords
Biological process	Pheromone response
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytogamy Inferred from direct assay PubMed 17369365. Source: SGD pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	integral to plasma membrane Inferred from direct assay Ref.1. Source: SGD
Molecular_function	mating-type a-factor pheromone receptor activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Pheromone a factor receptor		PRO_0000195079
Regions
	Topological domain	1 – 5	5	Extracellular Potential
	Transmembrane	6 – 23	18	Helical; Potential
	Topological domain	24 – 29	6	Cytoplasmic Potential
	Transmembrane	30 – 53	24	Helical; Potential
	Topological domain	54 – 70	17	Extracellular Potential
	Transmembrane	71 – 98	28	Helical; Potential
	Topological domain	99 – 116	18	Cytoplasmic Potential
	Transmembrane	117 – 134	18	Helical; Potential
	Topological domain	135 – 155	21	Extracellular Potential
	Transmembrane	156 – 183	28	Helical; Potential
	Topological domain	184 – 205	22	Cytoplasmic Potential
	Transmembrane	206 – 228	23	Helical; Potential
	Topological domain	229 – 266	38	Extracellular Potential
	Transmembrane	267 – 285	19	Helical; Potential
	Topological domain	286 – 470	185	Cytoplasmic Potential
	Region	300 – 470	171	Hydrophilic
Amino acid modifications
	Modified residue	430	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P06783 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 5DBF611076B8CA91	FASTA	470	53,708
	10 20 30 40 50 60 MSYKSAIIGL CLLAVILLAP PLAWHSHTKN IPAIILITWL LTMNLTCIVD AAIWSDDDFL 70 80 90 100 110 120 TRWDGKGWCD IVIKLQVGAN IGISCAVTNI IYNLHTILKA DSVLPDLSSW TKIVKDLVIS 130 140 150 160 170 180 LFTPVMVMGF SYLLQVFRYG IARYNGCQNL LSPTWITTVL YTMWMLIWSF VGAVYATLVL 190 200 210 220 230 240 FVFYKKRKDV RDILHCTNSG LNLTRFARLL IFCFIIILVM FPFSVYTFVQ DLQQVEGHYT 250 260 270 280 290 300 FKNTHSSTIW NTIIKFDPGR PIYNIWLYVL MSYLVFLIFG LGSDALHMYS KFLRSIKLGF 310 320 330 340 350 360 VLDMWKRFID KNKEKRVGIL LNKLSSRKES RNPFSTDSEN YISTCTENYS PCVGTPISQA 370 380 390 400 410 420 HFYVDYRIPD DPRKSQNKSK KYLFADKETD DILDEIDLKE SRHIPYVTQG QSFDDEISLG 430 440 450 460 470 GFSKVTLDYS EKLHNSASSN FEGESLCYSP ASKEENSSSN EHSSENTAGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone a factor: gene sequence and implications for the structure of the presumed receptor." Hagen D.C., McCaffrey G., Sprague G.F. Jr. Proc. Natl. Acad. Sci. U.S.A. 83:1418-1422(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes from Saccharomyces cerevisiae." Nakayama N., Miyajima A., Arai K. EMBO J. 4:2643-2648(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-430, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12239 Genomic DNA. Translation: AAA35113.1. X03011 Genomic DNA. Translation: CAA26795.1. X74151 Genomic DNA. Translation: CAA52261.1. Z28177 Genomic DNA. Translation: CAA82019.1. AY692742 Genomic DNA. Translation: AAT92761.1. BK006944 Genomic DNA. Translation: DAA08989.1.
PIR	B24670.
RefSeq	NP_012743.1. NM_001179744.1.
3D structure databases
ProteinModelPortal	P06783.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1146N.
IntAct	P06783. 1 interaction.
MINT	MINT-4788722.
STRING	4932.YKL178C.
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
DNASU	853677.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL178C; YKL178C; YKL178C.
GeneID	853677.
KEGG	sce:YKL178C.
Organism-specific databases
CYGD	YKL178c.
SGD	S000001661. STE3.
Phylogenomic databases
eggNOG	NOG71865.
HOGENOM	HOG000093298.
KO	K04627.
OMA	DILHCTN.
OrthoDB	EOG4CC79F.
Gene expression databases
Genevestigator	P06783.
GermOnline	YKL178C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001499. GPCR_STE3. [Graphical view]
Pfam	PF02076. STE3. 1 hit. [Graphical view]
PRINTS	PR00899. GPCRSTE3.
ProtoNet	Search...
Other
NextBio	974631.

Entry information

Entry name

STE3_YEAST

Accession

Primary (citable) accession number: P06783
Secondary accession number(s): D6VX23

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program