GTP-binding protein RHO2 precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P06781 (RHO2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 130. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTP-binding protein RHO2

Gene names

Name:	RHO2
Ordered Locus Names:	YNL090W
ORF Names:	N2237

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Miscellaneous	Present with 3870 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Palmitate Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Traceable author statement PubMed 9891811. Source: SGD establishment of cell polarity Traceable author statement PubMed 9891811. Source: SGD fungal-type cell wall organization Traceable author statement PubMed 9891811. Source: SGD microtubule-based process Traceable author statement PubMed 9891811. Source: SGD small GTPase mediated signal transduction Traceable author statement PubMed 9891811. Source: SGD
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro membrane Inferred from direct assay PubMed 18417612. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay PubMed 10526184 PubMed 11591390. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 189

189

GTP-binding protein RHO2

PRO_0000198946

Propeptide

190 – 192

Removed in mature form By similarity

PRO_0000281276

Regions

Nucleotide binding

14 – 21

GTP By similarity

Nucleotide binding

61 – 65

GTP By similarity

Nucleotide binding

119 – 122

GTP By similarity

Motif

36 – 44

Effector region By similarity

Amino acid modifications

Modified residue

189

Cysteine methyl ester By similarity

Lipidation

188

S-palmitoyl cysteine Probable

Lipidation

189

S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict

C → S in AAA34978. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P06781 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 468AA876DF13F69C
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FASTA

192

21,479

        10         20         30         40         50         60 
MSEKAVRRKL VIIGDGACGK TSLLYVFTLG KFPEQYHPTV FENYVTDCRV DGIKVSLTLW 

        70         80         90        100        110        120 
DTAGQEEYER LRPFSYSKAD IILIGFAVDN FESLINARTK WADEALRYCP DAPIVLVGLK 

       130        140        150        160        170        180 
KDLRQEAHFK ENATDEMVPI EDAKQVARAI GAKKYMECSA LTGEGVDDVF EVATRTSLLM 

       190 
KKEPGANCCI IL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae." Madaule P., Axel R., Myers A.M. Proc. Natl. Acad. Sci. U.S.A. 84:779-783(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Dissecting the architecture of a quantitative trait locus in yeast." Steinmetz L.M., Sinha H., Richards D.R., Spiegelman J.I., Oefner P.J., McCusker J.H., Davis R.W. Nature 416:326-330(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S96, SK1, YJM 1129, YJM 280, YJM 320, YJM 326, YJM 339, YJM 421 and YJM 627.
[3]	"Quantitative trait loci mapped to single-nucleotide resolution in yeast." Deutschbauer A.M., Davis R.W. Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SK1.
[4]	"The sequence of a 17,933 bp segment of Saccharomyces cerevisiae chromosome XIV contains the RHO2, TOP2, MKT1 and END3 genes and five new open reading frames." Soler-Mira A., Saiz J.E., Ballesta J.P.G., Remacha M.A. Yeast 12:485-491(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein palmitoylation in yeast." Roth A.F., Wan J., Bailey A.O., Sun B., Kuchar J.A., Green W.N., Phinney B.S., Yates J.R. III, Davis N.G. Cell 125:1003-1013(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15190 Genomic DNA. Translation: AAA34978.1. AF458969 Genomic DNA. Translation: AAM00517.1. AF458970 Genomic DNA. Translation: AAM00523.1. AF458971 Genomic DNA. Translation: AAM00529.1. AF458972 Genomic DNA. Translation: AAM00535.1. AF458973 Genomic DNA. Translation: AAM00541.1. AF458974 Genomic DNA. Translation: AAM00547.1. AF458976 Genomic DNA. Translation: AAM00559.1. AF458978 Genomic DNA. Translation: AAM00571.1. AF458981 Genomic DNA. Translation: AAM00589.1. DQ115393 Genomic DNA. Translation: AAZ22518.1. X89016 Genomic DNA. Translation: CAA61421.1. Z71366 Genomic DNA. Translation: CAA95965.1. BK006947 Genomic DNA. Translation: DAA10456.1.
PIR	TVBYH2. S57533.
RefSeq	NP_014309.3. NM_001182928.3.
3D structure databases
ProteinModelPortal	P06781.
SMR	P06781. Positions 5-178.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5750N.
IntAct	P06781. 6 interactions.
MINT	MINT-582528.
Proteomic databases
PaxDb	P06781.
PeptideAtlas	P06781.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL090W; YNL090W; YNL090W.
GeneID	855634.
KEGG	sce:YNL082W. sce:YNL090W.
Organism-specific databases
CYGD	YNL090w.
SGD	S000005034. RHO2.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00680000100556.
HOGENOM	HOG000233974.
KO	K07975. K10858.
OMA	DCRVDGK.
OrthoDB	EOG4H49D9.
Gene expression databases
ArrayExpress	P06781.
Genevestigator	P06781.
GermOnline	YNL090W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979851.

Entry information

Entry name

RHO2_YEAST

Accession

Primary (citable) accession number: P06781
Secondary accession number(s): D6W190, Q45TZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 130 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents