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Protein

DNA repair and recombination protein RAD52

Gene

RAD52

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA/DNA annealing activity Source: SGD
  • recombinase activity Source: SGD

GO - Biological processi

  • DNA amplification Source: SGD
  • DNA recombinase assembly Source: SGD
  • DNA strand renaturation Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • double-strand break repair via homologous recombination Source: SGD
  • double-strand break repair via single-strand annealing Source: SGD
  • meiotic joint molecule formation Source: SGD
  • postreplication repair Source: SGD
  • telomere maintenance via recombination Source: SGD

Keywordsi

Biological processDNA damage, DNA recombination, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-32633-MONOMER.
ReactomeiR-SCE-3108214. SUMOylation of DNA damage response and repair proteins.
R-SCE-5685938. HDR through Single Strand Annealing (SSA).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RAD52
Gene namesi
Name:RAD52
Ordered Locus Names:YML032C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML032C.
SGDiS000004494. RAD52.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromosome Source: SGD
  • nucleus Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738921 – 471DNA repair and recombination protein RAD52Add BLAST471

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP06778.

PTM databases

iPTMnetiP06778.

Interactioni

Subunit structurei

Part of a complex that includes RAD51, RAD52 and RAD59. Interacts with SAW1.2 Publications

Protein-protein interaction databases

BioGridi35137. 493 interactors.
DIPiDIP-3N.
IntActiP06778. 22 interactors.
MINTiMINT-444092.
STRINGi4932.YML032C.

Structurei

3D structure databases

ProteinModelPortaliP06778.
SMRiP06778.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RAD52 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000008766.
HOGENOMiHOG000154067.
InParanoidiP06778.
KOiK10873.
OMAiCLRCFGN.
OrthoDBiEOG092C4C2A.

Family and domain databases

InterProiView protein in InterPro
IPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad59_Rad22.
PANTHERiPTHR12132. PTHR12132. 1 hit.
PfamiView protein in Pfam
PF04098. Rad52_Rad22. 1 hit.
TIGRFAMsiTIGR00607. rad52. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P06778-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG
60 70 80 90 100
WRVINLANQI FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG
110 120 130 140 150
TYREDIGYGT VENERRKPAA FERAKKSAVT DALKRSLRGF GNALGNCLYD
160 170 180 190 200
KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS ESSRTNTLHE NQEQQQYPNK
210 220 230 240 250
RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS KNSSNKDTDL
260 270 280 290 300
KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK
310 320 330 340 350
QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV
360 370 380 390 400
DQTTSKHIPA SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML
410 420 430 440 450
EGAGNQVPRE TTPIKTNATA FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ
460 470
RSTRREVGRP KINPLHARKP T
Length:471
Mass (Da):52,404
Last modified:June 27, 2006 - v2
Checksum:i4F5D07480A05B564
GO
Isoform 2 (identifier: P06778-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.

Note: Produced by alternative initiation at Met-5 of isoform 1.
Show »
Length:467
Mass (Da):51,916
Checksum:i857A5AD7AA014748
GO
Isoform 3 (identifier: P06778-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Note: Produced by alternative initiation at Met-7 of isoform 1.
Show »
Length:465
Mass (Da):51,670
Checksum:i426DFDCAB969A5A4
GO

Sequence cautioni

The sequence AAA50352 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAT93163 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA86623 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0196131 – 6Missing in isoform 3. Curated6
Alternative sequenceiVSP_0196121 – 4Missing in isoform 2. Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10249 Genomic DNA. Translation: AAA50352.1. Different initiation.
Z46659 Genomic DNA. Translation: CAA86623.1. Different initiation.
AY693144 Genomic DNA. Translation: AAT93163.1. Different initiation.
BK006946 Genomic DNA. Translation: DAA09866.1.
PIRiA23282.
RefSeqiNP_013680.2. NM_001182390.1. [P06778-1]

Genome annotation databases

EnsemblFungiiYML032C; YML032C; YML032C. [P06778-1]
GeneIDi854976.
KEGGisce:YML032C.

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRAD52_YEAST
AccessioniPrimary (citable) accession number: P06778
Secondary accession number(s): D6VZE2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: June 27, 2006
Last modified: July 5, 2017
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names