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P06778

- RAD52_YEAST

UniProt

P06778 - RAD52_YEAST

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Protein
DNA repair and recombination protein RAD52
Gene
RAD52, YML032C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.

GO - Molecular functioni

  1. recombinase activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. DNA amplification Source: SGD
  2. DNA recombinase assembly Source: SGD
  3. DNA strand renaturation Source: SGD
  4. double-strand break repair via break-induced replication Source: SGD
  5. double-strand break repair via homologous recombination Source: SGD
  6. double-strand break repair via single-strand annealing Source: SGD
  7. meiotic joint molecule formation Source: SGD
  8. postreplication repair Source: SGD
  9. telomere maintenance via recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-32633-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RAD52
Gene namesi
Name:RAD52
Ordered Locus Names:YML032C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML032c.
SGDiS000004494. RAD52.

Subcellular locationi

GO - Cellular componenti

  1. nuclear chromosome Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471DNA repair and recombination protein RAD52
PRO_0000173892Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06778.
PaxDbiP06778.
PeptideAtlasiP06778.

Expressioni

Gene expression databases

GenevestigatoriP06778.

Interactioni

Subunit structurei

Part of a complex that includes RAD51, RAD52 and RAD59. Interacts with SAW1.2 Publications

Protein-protein interaction databases

BioGridi35137. 414 interactions.
DIPiDIP-3N.
IntActiP06778. 20 interactions.
MINTiMINT-444092.
STRINGi4932.YML032C.

Structurei

3D structure databases

ProteinModelPortaliP06778.
SMRiP06778. Positions 19-165.

Family & Domainsi

Sequence similaritiesi

Belongs to the RAD52 family.

Phylogenomic databases

eggNOGiCOG5055.
GeneTreeiENSGT00390000008766.
HOGENOMiHOG000154067.
KOiK10873.
OrthoDBiEOG7F7WMJ.

Family and domain databases

InterProiIPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad22.
[Graphical view]
PANTHERiPTHR12132. PTHR12132. 1 hit.
PfamiPF04098. Rad52_Rad22. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00607. rad52. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: P06778-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG    50
WRVINLANQI FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG 100
TYREDIGYGT VENERRKPAA FERAKKSAVT DALKRSLRGF GNALGNCLYD 150
KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS ESSRTNTLHE NQEQQQYPNK 200
RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS KNSSNKDTDL 250
KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK 300
QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV 350
DQTTSKHIPA SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML 400
EGAGNQVPRE TTPIKTNATA FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ 450
RSTRREVGRP KINPLHARKP T 471
Length:471
Mass (Da):52,404
Last modified:June 27, 2006 - v2
Checksum:i4F5D07480A05B564
GO
Isoform 2 (identifier: P06778-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.

Note: Produced by alternative initiation at Met-5 of isoform 1.

Show »
Length:467
Mass (Da):51,916
Checksum:i857A5AD7AA014748
GO
Isoform 3 (identifier: P06778-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Note: Produced by alternative initiation at Met-7 of isoform 1.

Show »
Length:465
Mass (Da):51,670
Checksum:i426DFDCAB969A5A4
GO

Sequence cautioni

The sequence AAA50352.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAT93163.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence CAA86623.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66Missing in isoform 3.
VSP_019613
Alternative sequencei1 – 44Missing in isoform 2.
VSP_019612

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10249 Genomic DNA. Translation: AAA50352.1. Different initiation.
Z46659 Genomic DNA. Translation: CAA86623.1. Different initiation.
AY693144 Genomic DNA. Translation: AAT93163.1. Different initiation.
BK006946 Genomic DNA. Translation: DAA09866.1.
PIRiA23282.
RefSeqiNP_013680.2. NM_001182390.1. [P06778-1]

Genome annotation databases

EnsemblFungiiYML032C; YML032C; YML032C. [P06778-1]
GeneIDi854976.
KEGGisce:YML032C.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10249 Genomic DNA. Translation: AAA50352.1 . Different initiation.
Z46659 Genomic DNA. Translation: CAA86623.1 . Different initiation.
AY693144 Genomic DNA. Translation: AAT93163.1 . Different initiation.
BK006946 Genomic DNA. Translation: DAA09866.1 .
PIRi A23282.
RefSeqi NP_013680.2. NM_001182390.1. [P06778-1 ]

3D structure databases

ProteinModelPortali P06778.
SMRi P06778. Positions 19-165.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35137. 414 interactions.
DIPi DIP-3N.
IntActi P06778. 20 interactions.
MINTi MINT-444092.
STRINGi 4932.YML032C.

Proteomic databases

MaxQBi P06778.
PaxDbi P06778.
PeptideAtlasi P06778.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML032C ; YML032C ; YML032C . [P06778-1 ]
GeneIDi 854976.
KEGGi sce:YML032C.

Organism-specific databases

CYGDi YML032c.
SGDi S000004494. RAD52.

Phylogenomic databases

eggNOGi COG5055.
GeneTreei ENSGT00390000008766.
HOGENOMi HOG000154067.
KOi K10873.
OrthoDBi EOG7F7WMJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-32633-MONOMER.

Miscellaneous databases

NextBioi 978085.

Gene expression databases

Genevestigatori P06778.

Family and domain databases

InterProi IPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad22.
[Graphical view ]
PANTHERi PTHR12132. PTHR12132. 1 hit.
Pfami PF04098. Rad52_Rad22. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00607. rad52. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the RAD52 gene in Saccharomyces cerevisiae."
    Adzuma K., Ogawa T., Ogawa H.
    Mol. Cell. Biol. 4:2735-2744(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The Rad52-Rad59 complex interacts with Rad51 and replication protein A."
    Davis A.P., Symington L.S.
    DNA Repair 2:1127-1134(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD51 AND RAD59.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Multiple start codons and phosphorylation result in discrete Rad52 protein species."
    Antunez de Mayolo A., Lisby M., Erdeniz N., Thybo T., Mortensen U.H., Rothstein R.
    Nucleic Acids Res. 34:2587-2597(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INITIATION SITES, PHOSPHORYLATION.
  8. "Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates."
    Li F., Dong J., Pan X., Oum J.-H., Boeke J.D., Lee S.E.
    Mol. Cell 30:325-335(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SAW1.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAD52_YEAST
AccessioniPrimary (citable) accession number: P06778
Secondary accession number(s): D6VZE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: June 27, 2006
Last modified: September 3, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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