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P06778 (RAD52_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair and recombination protein RAD52
Gene names
Name:RAD52
Ordered Locus Names:YML032C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.

Subunit structure

Part of a complex that includes RAD51, RAD52 and RAD59. Interacts with SAW1. Ref.5 Ref.8

Subcellular location

Nucleus.

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RAD52 family.

Sequence caution

The sequence AAA50352.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAT93163.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence CAA86623.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 3 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: P06778-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P06778-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.
Note: Produced by alternative initiation at Met-5 of isoform 1.
Isoform 3 (identifier: P06778-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.
Note: Produced by alternative initiation at Met-7 of isoform 1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 471471DNA repair and recombination protein RAD52
PRO_0000173892

Natural variations

Alternative sequence1 – 66Missing in isoform 3.
VSP_019613
Alternative sequence1 – 44Missing in isoform 2.
VSP_019612

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 27, 2006. Version 2.
Checksum: 4F5D07480A05B564

FASTA47152,404
        10         20         30         40         50         60 
MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG WRVINLANQI 

        70         80         90        100        110        120 
FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG TYREDIGYGT VENERRKPAA 

       130        140        150        160        170        180 
FERAKKSAVT DALKRSLRGF GNALGNCLYD KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS 

       190        200        210        220        230        240 
ESSRTNTLHE NQEQQQYPNK RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS 

       250        260        270        280        290        300 
KNSSNKDTDL KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK 

       310        320        330        340        350        360 
QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV DQTTSKHIPA 

       370        380        390        400        410        420 
SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML EGAGNQVPRE TTPIKTNATA 

       430        440        450        460        470 
FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ RSTRREVGRP KINPLHARKP T 

« Hide

Isoform 2 [UniParc].

Checksum: 857A5AD7AA014748
Show »

FASTA46751,916
Isoform 3 [UniParc].

Checksum: 426DFDCAB969A5A4
Show »

FASTA46551,670

References

« Hide 'large scale' references
[1]"Primary structure of the RAD52 gene in Saccharomyces cerevisiae."
Adzuma K., Ogawa T., Ogawa H.
Mol. Cell. Biol. 4:2735-2744(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The Rad52-Rad59 complex interacts with Rad51 and replication protein A."
Davis A.P., Symington L.S.
DNA Repair 2:1127-1134(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RAD51 AND RAD59.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Multiple start codons and phosphorylation result in discrete Rad52 protein species."
Antunez de Mayolo A., Lisby M., Erdeniz N., Thybo T., Mortensen U.H., Rothstein R.
Nucleic Acids Res. 34:2587-2597(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF INITIATION SITES, PHOSPHORYLATION.
[8]"Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates."
Li F., Dong J., Pan X., Oum J.-H., Boeke J.D., Lee S.E.
Mol. Cell 30:325-335(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SAW1.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M10249 Genomic DNA. Translation: AAA50352.1. Different initiation.
Z46659 Genomic DNA. Translation: CAA86623.1. Different initiation.
AY693144 Genomic DNA. Translation: AAT93163.1. Different initiation.
BK006946 Genomic DNA. Translation: DAA09866.1.
PIRA23282.
RefSeqNP_013680.2. NM_001182390.1.

3D structure databases

ProteinModelPortalP06778.
SMRP06778. Positions 19-165.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35137. 409 interactions.
DIPDIP-3N.
IntActP06778. 20 interactions.
MINTMINT-444092.
STRING4932.YML032C.

Proteomic databases

PaxDbP06778.
PeptideAtlasP06778.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML032C; YML032C; YML032C. [P06778-1]
GeneID854976.
KEGGsce:YML032C.

Organism-specific databases

CYGDYML032c.
SGDS000004494. RAD52.

Phylogenomic databases

eggNOGCOG5055.
GeneTreeENSGT00390000008766.
HOGENOMHOG000154067.
KOK10873.
OrthoDBEOG7F7WMJ.

Enzyme and pathway databases

BioCycYEAST:G3O-32633-MONOMER.

Gene expression databases

GenevestigatorP06778.

Family and domain databases

InterProIPR004585. DNA_recomb/repair_Rad52.
IPR007232. Rad52_Rad22.
[Graphical view]
PANTHERPTHR12132. PTHR12132. 1 hit.
PfamPF04098. Rad52_Rad22. 1 hit.
[Graphical view]
TIGRFAMsTIGR00607. rad52. 1 hit.
ProtoNetSearch...

Other

NextBio978085.

Entry information

Entry nameRAD52_YEAST
AccessionPrimary (citable) accession number: P06778
Secondary accession number(s): D6VZE2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: June 27, 2006
Last modified: April 16, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families