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P06778

- RAD52_YEAST

UniProt

P06778 - RAD52_YEAST

Protein

DNA repair and recombination protein RAD52

Gene

RAD52

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.

    GO - Molecular functioni

    1. recombinase activity Source: SGD

    GO - Biological processi

    1. DNA amplification Source: SGD
    2. DNA recombinase assembly Source: SGD
    3. DNA strand renaturation Source: SGD
    4. double-strand break repair via break-induced replication Source: SGD
    5. double-strand break repair via homologous recombination Source: SGD
    6. double-strand break repair via single-strand annealing Source: SGD
    7. meiotic joint molecule formation Source: SGD
    8. postreplication repair Source: SGD
    9. telomere maintenance via recombination Source: SGD

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32633-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair and recombination protein RAD52
    Gene namesi
    Name:RAD52
    Ordered Locus Names:YML032C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML032c.
    SGDiS000004494. RAD52.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear chromosome Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 471471DNA repair and recombination protein RAD52PRO_0000173892Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP06778.
    PaxDbiP06778.
    PeptideAtlasiP06778.

    Expressioni

    Gene expression databases

    GenevestigatoriP06778.

    Interactioni

    Subunit structurei

    Part of a complex that includes RAD51, RAD52 and RAD59. Interacts with SAW1.2 Publications

    Protein-protein interaction databases

    BioGridi35137. 414 interactions.
    DIPiDIP-3N.
    IntActiP06778. 20 interactions.
    MINTiMINT-444092.
    STRINGi4932.YML032C.

    Structurei

    3D structure databases

    ProteinModelPortaliP06778.
    SMRiP06778. Positions 19-165.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RAD52 family.Curated

    Phylogenomic databases

    eggNOGiCOG5055.
    GeneTreeiENSGT00390000008766.
    HOGENOMiHOG000154067.
    KOiK10873.
    OrthoDBiEOG7F7WMJ.

    Family and domain databases

    InterProiIPR004585. DNA_recomb/repair_Rad52.
    IPR007232. Rad52_Rad22.
    [Graphical view]
    PANTHERiPTHR12132. PTHR12132. 1 hit.
    PfamiPF04098. Rad52_Rad22. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00607. rad52. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative initiation. Align

    Isoform 1 (identifier: P06778-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG    50
    WRVINLANQI FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG 100
    TYREDIGYGT VENERRKPAA FERAKKSAVT DALKRSLRGF GNALGNCLYD 150
    KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS ESSRTNTLHE NQEQQQYPNK 200
    RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS KNSSNKDTDL 250
    KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK 300
    QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV 350
    DQTTSKHIPA SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML 400
    EGAGNQVPRE TTPIKTNATA FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ 450
    RSTRREVGRP KINPLHARKP T 471
    Length:471
    Mass (Da):52,404
    Last modified:June 27, 2006 - v2
    Checksum:i4F5D07480A05B564
    GO
    Isoform 2 (identifier: P06778-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-4: Missing.

    Note: Produced by alternative initiation at Met-5 of isoform 1.

    Show »
    Length:467
    Mass (Da):51,916
    Checksum:i857A5AD7AA014748
    GO
    Isoform 3 (identifier: P06778-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-6: Missing.

    Note: Produced by alternative initiation at Met-7 of isoform 1.

    Show »
    Length:465
    Mass (Da):51,670
    Checksum:i426DFDCAB969A5A4
    GO

    Sequence cautioni

    The sequence AAA50352.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence AAT93163.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence CAA86623.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 66Missing in isoform 3. CuratedVSP_019613
    Alternative sequencei1 – 44Missing in isoform 2. CuratedVSP_019612

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10249 Genomic DNA. Translation: AAA50352.1. Different initiation.
    Z46659 Genomic DNA. Translation: CAA86623.1. Different initiation.
    AY693144 Genomic DNA. Translation: AAT93163.1. Different initiation.
    BK006946 Genomic DNA. Translation: DAA09866.1.
    PIRiA23282.
    RefSeqiNP_013680.2. NM_001182390.1. [P06778-1]

    Genome annotation databases

    EnsemblFungiiYML032C; YML032C; YML032C. [P06778-1]
    GeneIDi854976.
    KEGGisce:YML032C.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10249 Genomic DNA. Translation: AAA50352.1 . Different initiation.
    Z46659 Genomic DNA. Translation: CAA86623.1 . Different initiation.
    AY693144 Genomic DNA. Translation: AAT93163.1 . Different initiation.
    BK006946 Genomic DNA. Translation: DAA09866.1 .
    PIRi A23282.
    RefSeqi NP_013680.2. NM_001182390.1. [P06778-1 ]

    3D structure databases

    ProteinModelPortali P06778.
    SMRi P06778. Positions 19-165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35137. 414 interactions.
    DIPi DIP-3N.
    IntActi P06778. 20 interactions.
    MINTi MINT-444092.
    STRINGi 4932.YML032C.

    Proteomic databases

    MaxQBi P06778.
    PaxDbi P06778.
    PeptideAtlasi P06778.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML032C ; YML032C ; YML032C . [P06778-1 ]
    GeneIDi 854976.
    KEGGi sce:YML032C.

    Organism-specific databases

    CYGDi YML032c.
    SGDi S000004494. RAD52.

    Phylogenomic databases

    eggNOGi COG5055.
    GeneTreei ENSGT00390000008766.
    HOGENOMi HOG000154067.
    KOi K10873.
    OrthoDBi EOG7F7WMJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32633-MONOMER.

    Miscellaneous databases

    NextBioi 978085.

    Gene expression databases

    Genevestigatori P06778.

    Family and domain databases

    InterProi IPR004585. DNA_recomb/repair_Rad52.
    IPR007232. Rad52_Rad22.
    [Graphical view ]
    PANTHERi PTHR12132. PTHR12132. 1 hit.
    Pfami PF04098. Rad52_Rad22. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00607. rad52. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the RAD52 gene in Saccharomyces cerevisiae."
      Adzuma K., Ogawa T., Ogawa H.
      Mol. Cell. Biol. 4:2735-2744(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The Rad52-Rad59 complex interacts with Rad51 and replication protein A."
      Davis A.P., Symington L.S.
      DNA Repair 2:1127-1134(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAD51 AND RAD59.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Multiple start codons and phosphorylation result in discrete Rad52 protein species."
      Antunez de Mayolo A., Lisby M., Erdeniz N., Thybo T., Mortensen U.H., Rothstein R.
      Nucleic Acids Res. 34:2587-2597(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF INITIATION SITES, PHOSPHORYLATION.
    8. "Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates."
      Li F., Dong J., Pan X., Oum J.-H., Boeke J.D., Lee S.E.
      Mol. Cell 30:325-335(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SAW1.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRAD52_YEAST
    AccessioniPrimary (citable) accession number: P06778
    Secondary accession number(s): D6VZE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1080 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3