DNA repair and recombination protein RAD52 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P06778 (RAD52_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA repair and recombination protein RAD52

Gene names

Name:	RAD52
Ordered Locus Names:	YML032C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
Subunit structure	Part of a complex that includes RAD51, RAD52 and RAD59. Interacts with SAW1. Ref.5 Ref.8
Subcellular location	Nucleus.
Miscellaneous	Present with 1080 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the RAD52 family.
Sequence caution	The sequence AAA50352.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAT93163.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAA86623.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Cellular component	Nucleus
Coding sequence diversity	Alternative initiation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA strand renaturation Inferred from direct assay PubMed 9619627. Source: SGD double-strand break repair via break-induced replication Traceable author statement PubMed 11459961. Source: SGD double-strand break repair via single-strand annealing Inferred from genetic interaction PubMed 11606529. Source: SGD meiotic DNA recombinase assembly Traceable author statement PubMed 11459983. Source: SGD meiotic joint molecule formation Inferred from mutant phenotype PubMed 18313389. Source: SGD postreplication repair Inferred from mutant phenotype PubMed 7038396. Source: SGD telomere maintenance via recombination Inferred from mutant phenotype PubMed 11238918. Source: SGD
Cellular_component	nuclear chromosome Inferred from direct assay PubMed 12766777. Source: SGD
Molecular_function	recombinase activity Inferred from direct assay PubMed 15205482. Source: SGD
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: P06778-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P06778-2) The sequence of this isoform differs from the canonical sequence as follows: 1-4: Missing.
Note: Produced by alternative initiation at Met-5 of isoform 1.

Isoform 3 (identifier: P06778-3) The sequence of this isoform differs from the canonical sequence as follows: 1-6: Missing.
Note: Produced by alternative initiation at Met-7 of isoform 1.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 471

471

DNA repair and recombination protein RAD52

PRO_0000173892

Amino acid modifications

Modified residue

302

Phosphoserine Ref.9

Modified residue

304

Phosphothreonine Ref.9

Modified residue

306

Phosphoserine Ref.9

Modified residue

307

Phosphoserine Ref.9

Natural variations

Alternative sequence

1 – 6

Missing in isoform 3.

VSP_019613

Alternative sequence

1 – 4

Missing in isoform 2.

VSP_019612

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 4F5D07480A05B564 Show »	FASTA	471	52,404
10 20 30 40 50 60 MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG WRVINLANQI 70 80 90 100 110 120 FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG TYREDIGYGT VENERRKPAA 130 140 150 160 170 180 FERAKKSAVT DALKRSLRGF GNALGNCLYD KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS 190 200 210 220 230 240 ESSRTNTLHE NQEQQQYPNK RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS 250 260 270 280 290 300 KNSSNKDTDL KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK 310 320 330 340 350 360 QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV DQTTSKHIPA 370 380 390 400 410 420 SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML EGAGNQVPRE TTPIKTNATA 430 440 450 460 470 FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ RSTRREVGRP KINPLHARKP T « Hide
Isoform 2 [UniParc]. Checksum: 857A5AD7AA014748 Show »	FASTA	467	51,916
10 20 30 40 50 60 MDMDEKKPVF GNHSEDIQTK LDKKLGPEYI SKRVGFGTSR IAYIEGWRVI NLANQIFGYN 70 80 90 100 110 120 GWSTEVKSVV IDFLDERQGK FSIGCTAIVR VTLTSGTYRE DIGYGTVENE RRKPAAFERA 130 140 150 160 170 180 KKSAVTDALK RSLRGFGNAL GNCLYDKDFL AKIDKVKFDP PDFDENNLFR PTDEISESSR 190 200 210 220 230 240 TNTLHENQEQ QQYPNKRRQL TKVTNTNPDS TKNLVKIENT VSRGTPMMAA PAEANSKNSS 250 260 270 280 290 300 NKDTDLKSLD ASKQDQDDLL DDSLMFSDDF QDDDLINMGN TNSNVLTTEK DPVVAKQSPT 310 320 330 340 350 360 ASSNPEAEQI TFVTAKAATS VQNERYIGEE SIFDPKYQAQ SIRHTVDQTT SKHIPASVLK 370 380 390 400 410 420 DKTMTTARDS VYEKFAPKGK QLSMKNNDKE LGPHMLEGAG NQVPRETTPI KTNATAFPPA 430 440 450 460 AAPRFAPPSK VVHPNGNGAV PAVPQQRSTR REVGRPKINP LHARKPT « Hide
Isoform 3 [UniParc]. Checksum: 426DFDCAB969A5A4 Show »	FASTA	465	51,670
10 20 30 40 50 60 MDEKKPVFGN HSEDIQTKLD KKLGPEYISK RVGFGTSRIA YIEGWRVINL ANQIFGYNGW 70 80 90 100 110 120 STEVKSVVID FLDERQGKFS IGCTAIVRVT LTSGTYREDI GYGTVENERR KPAAFERAKK 130 140 150 160 170 180 SAVTDALKRS LRGFGNALGN CLYDKDFLAK IDKVKFDPPD FDENNLFRPT DEISESSRTN 190 200 210 220 230 240 TLHENQEQQQ YPNKRRQLTK VTNTNPDSTK NLVKIENTVS RGTPMMAAPA EANSKNSSNK 250 260 270 280 290 300 DTDLKSLDAS KQDQDDLLDD SLMFSDDFQD DDLINMGNTN SNVLTTEKDP VVAKQSPTAS 310 320 330 340 350 360 SNPEAEQITF VTAKAATSVQ NERYIGEESI FDPKYQAQSI RHTVDQTTSK HIPASVLKDK 370 380 390 400 410 420 TMTTARDSVY EKFAPKGKQL SMKNNDKELG PHMLEGAGNQ VPRETTPIKT NATAFPPAAA 430 440 450 460 PRFAPPSKVV HPNGNGAVPA VPQQRSTRRE VGRPKINPLH ARKPT « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the RAD52 gene in Saccharomyces cerevisiae." Adzuma K., Ogawa T., Ogawa H. Mol. Cell. Biol. 4:2735-2744(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The Rad52-Rad59 complex interacts with Rad51 and replication protein A." Davis A.P., Symington L.S. DNA Repair 2:1127-1134(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RAD51 AND RAD59.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Multiple start codons and phosphorylation result in discrete Rad52 protein species." Antunez de Mayolo A., Lisby M., Erdeniz N., Thybo T., Mortensen U.H., Rothstein R. Nucleic Acids Res. 34:2587-2597(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF INITIATION SITES, PHOSPHORYLATION.
[8]	"Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates." Li F., Dong J., Pan X., Oum J.-H., Boeke J.D., Lee S.E. Mol. Cell 30:325-335(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SAW1.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302; THR-304; SER-306 AND SER-307, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M10249 Genomic DNA. Translation: AAA50352.1. Different initiation. Z46659 Genomic DNA. Translation: CAA86623.1. Different initiation. AY693144 Genomic DNA. Translation: AAT93163.1. Different initiation. BK006946 Genomic DNA. Translation: DAA09866.1.
PIR	A23282.
RefSeq	NP_013680.2. NM_001182390.1.
3D structure databases
ProteinModelPortal	P06778.
SMR	P06778. Positions 19-165.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3N.
IntAct	P06778. 22 interactions.
MINT	MINT-444092.
STRING	4932.YML032C.
Proteomic databases
PaxDb	P06778.
PeptideAtlas	P06778.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML032C; YML032C; YML032C.
GeneID	854976.
KEGG	sce:YML032C.
Organism-specific databases
CYGD	YML032c.
SGD	S000004494. RAD52.
Phylogenomic databases
eggNOG	COG5055.
GeneTree	ENSGT00390000008766.
HOGENOM	HOG000154067.
KO	K10873.
OMA	GYGTVEN.
OrthoDB	EOG4R7ZMC.
Gene expression databases
Genevestigator	P06778.
GermOnline	YML032C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004585. DNA_recomb/repair_Rad52. IPR007232. Rad52_Rad22. [Graphical view]
PANTHER	PTHR12132. PTHR12132. 1 hit.
Pfam	PF04098. Rad52_Rad22. 1 hit. [Graphical view]
TIGRFAMs	TIGR00607. rad52. 1 hit.
ProtoNet	Search...
Other
NextBio	978085.

Entry information

Entry name

RAD52_YEAST

Accession

Primary (citable) accession number: P06778
Secondary accession number(s): D6VZE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	June 27, 2006
Last modified:	May 1, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents