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Protein

DNA repair protein RAD1

Gene

RAD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Along with RAD10 forms an endonuclease that specifically degrades single-stranded DNA.1 Publication

GO - Molecular functioni

  • damaged DNA binding Source: GO_Central
  • endonuclease activity Source: UniProtKB-KW
  • single-stranded DNA binding Source: SGD

GO - Biological processi

  • DNA amplification Source: SGD
  • double-strand break repair via homologous recombination Source: GO_Central
  • double-strand break repair via single-strand annealing, removal of nonhomologous ends Source: SGD
  • meiotic mismatch repair Source: SGD
  • mitotic recombination Source: SGD
  • nucleotide-excision repair, DNA incision, 5'-to lesion Source: SGD
  • nucleotide-excision repair involved in interstrand cross-link repair Source: GO_Central
  • removal of nonhomologous ends Source: SGD
  • resolution of meiotic recombination intermediates Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33940-MONOMER.
ReactomeiR-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-6782135. Dual incision in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD1
Gene namesi
Name:RAD1
Ordered Locus Names:YPL022W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL022W.
SGDiS000005943. RAD1.

Subcellular locationi

GO - Cellular componenti

  • nucleotide-excision repair factor 1 complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11001100DNA repair protein RAD1PRO_0000198857Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei613 – 6131PhosphoserineCombined sources
Modified residuei1071 – 10711PhosphoserineCombined sources
Modified residuei1072 – 10721PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP06777.
PRIDEiP06777.

PTM databases

iPTMnetiP06777.

Interactioni

Subunit structurei

Component of the nucleotide excision repair factor 1 (NEF1) complex consisting of RAD1, RAD10 and RAD14. Interacts with SAW1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD10P068389EBI-14752,EBI-14637
RAD14P285193EBI-14752,EBI-14641
SAW1P397354EBI-14752,EBI-20627

Protein-protein interaction databases

BioGridi36155. 178 interactions.
DIPiDIP-2424N.
IntActiP06777. 19 interactions.
MINTiMINT-536819.

Structurei

3D structure databases

ProteinModelPortaliP06777.
SMRiP06777. Positions 822-976.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini821 – 90181ERCC4Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 110110Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi516 – 57661Arg/Lys-rich (basic)Add
BLAST
Compositional biasi595 – 68995Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi1041 – 110060Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the XPF family.Curated
Contains 1 ERCC4 domain.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004394.
HOGENOMiHOG000202204.
InParanoidiP06777.
KOiK10848.
OMAiRKYSESP.
OrthoDBiEOG092C0B3U.

Family and domain databases

Gene3Di3.40.50.10130. 1 hit.
InterProiIPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR011335. Restrct_endonuc-II-like.
IPR010994. RuvA_2-like.
IPR006167. XPF.
[Graphical view]
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF52980. SSF52980. 1 hit.
TIGRFAMsiTIGR00596. rad1. 1 hit.

Sequencei

Sequence statusi: Complete.

P06777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQLFYQGDS DDELQEELTR QTTQASQSSK IKNEDEPDDS NHLNEVENED
60 70 80 90 100
SKVLDDDAVL YPLIPNEPDD IETSKPNIND IRPVDIQLTL PLPFQQKVVE
110 120 130 140 150
NSLITEDALI IMGKGLGLLD IVANLLHVLA TPTSINGQLK RALVLVLNAK
160 170 180 190 200
PIDNVRIKEA LEELSWFSNT GKDDDDTAVE SDDELFERPF NVVTADSLSI
210 220 230 240 250
EKRRKLYISG GILSITSRIL IVDLLSGIVH PNRVTGMLVL NADSLRHNSN
260 270 280 290 300
ESFILEIYRS KNTWGFIKAF SEAPETFVME FSPLRTKMKE LRLKNVLLWP
310 320 330 340 350
RFRVEVSSCL NATNKTSHNK VIEVKVSLTN SMSQIQFGLM ECLKKCIAEL
360 370 380 390 400
SRKNPELALD WWNMENVLDI NFIRSIDSVM VPNWHRISYE SKQLVKDIRF
410 420 430 440 450
LRHLLKMLVT SDAVDFFGEI QLSLDANKPS VSRKYSESPW LLVDEAQLVI
460 470 480 490 500
SYAKKRIFYK NEYTLEENPK WEQLIHILHD ISHERMTNHL QGPTLVACSD
510 520 530 540 550
NLTCLELAKV LNASNKKRGV RQVLLNKLKW YRKQREETKK LVKEVQSQDT
560 570 580 590 600
FPENATLNVS STFSKEQVTT KRRRTRGASQ VAAVEKLRNA GTNVDMEVVF
610 620 630 640 650
EDHKLSEEIK KGSGDDLDDG QEENAANDSK IFEIQEQENE ILIDDGDAEF
660 670 680 690 700
DNGELEYVGD LPQHITTHFN KDLWAEHCNE YEYVDRQDEI LISTFKSLND
710 720 730 740 750
NCSLQEMMPS YIIMFEPDIS FIRQIEVYKA IVKDLQPKVY FMYYGESIEE
760 770 780 790 800
QSHLTAIKRE KDAFTKLIRE NANLSHHFET NEDLSHYKNL AERKLKLSKL
810 820 830 840 850
RKSNTRNAGG QQGFHNLTQD VVIVDTREFN ASLPGLLYRY GIRVIPCMLT
860 870 880 890 900
VGDYVITPDI CLERKSISDL IGSLQNNRLA NQCKKMLKYY AYPTLLIEFD
910 920 930 940 950
EGQSFSLEPF SERRNYKNKD ISTVHPISSK LSQDEIQLKL AKLVLRFPTL
960 970 980 990 1000
KIIWSSSPLQ TVNIILELKL GREQPDPSNA VILGTNKVRS DFNSTAKGLK
1010 1020 1030 1040 1050
DGDNESKFKR LLNVPGVSKI DYFNLRKKIK SFNKLQKLSW NEINELINDE
1060 1070 1080 1090 1100
DLTDRIYYFL RTEKEEQEQE STDENLESPG KTTDDNALHD HHNDVPEAPV
Length:1,100
Mass (Da):126,370
Last modified:January 1, 1988 - v1
Checksum:i06FDA601F3F59B10
GO

Sequence cautioni

The sequence AAA34929 differs from that shown. Reason: Frameshift at positions 923, 934 and 948. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2231D → N in AAA34929 (PubMed:6095044).Curated
Sequence conflicti883 – 8831C → Y in AAA34929 (PubMed:6095044).Curated
Sequence conflicti886 – 8861M → I in AAA34929 (PubMed:6095044).Curated
Sequence conflicti912 – 9121E → K in AAA34929 (PubMed:6095044).Curated
Sequence conflicti924 – 9241V → I in AAA34929 (PubMed:6095044).Curated
Sequence conflicti1016 – 10161G → R in AAA34929 (PubMed:6095044).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15435 Genomic DNA. Translation: AAA34934.1.
U36624 Genomic DNA. Translation: AAB68165.1.
K02070 Genomic DNA. Translation: AAA34929.1. Frameshift.
BK006949 Genomic DNA. Translation: DAA11406.1.
PIRiA26129. DDBYD1.
RefSeqiNP_015303.1. NM_001183836.1.

Genome annotation databases

EnsemblFungiiYPL022W; YPL022W; YPL022W.
GeneIDi856085.
KEGGisce:YPL022W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15435 Genomic DNA. Translation: AAA34934.1.
U36624 Genomic DNA. Translation: AAB68165.1.
K02070 Genomic DNA. Translation: AAA34929.1. Frameshift.
BK006949 Genomic DNA. Translation: DAA11406.1.
PIRiA26129. DDBYD1.
RefSeqiNP_015303.1. NM_001183836.1.

3D structure databases

ProteinModelPortaliP06777.
SMRiP06777. Positions 822-976.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36155. 178 interactions.
DIPiDIP-2424N.
IntActiP06777. 19 interactions.
MINTiMINT-536819.

PTM databases

iPTMnetiP06777.

Proteomic databases

MaxQBiP06777.
PRIDEiP06777.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL022W; YPL022W; YPL022W.
GeneIDi856085.
KEGGisce:YPL022W.

Organism-specific databases

EuPathDBiFungiDB:YPL022W.
SGDiS000005943. RAD1.

Phylogenomic databases

GeneTreeiENSGT00390000004394.
HOGENOMiHOG000202204.
InParanoidiP06777.
KOiK10848.
OMAiRKYSESP.
OrthoDBiEOG092C0B3U.

Enzyme and pathway databases

BioCyciYEAST:G3O-33940-MONOMER.
ReactomeiR-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-6782135. Dual incision in TC-NER.

Miscellaneous databases

PROiP06777.

Family and domain databases

Gene3Di3.40.50.10130. 1 hit.
InterProiIPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR011335. Restrct_endonuc-II-like.
IPR010994. RuvA_2-like.
IPR006167. XPF.
[Graphical view]
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF52980. SSF52980. 1 hit.
TIGRFAMsiTIGR00596. rad1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRAD1_YEAST
AccessioniPrimary (citable) accession number: P06777
Secondary accession number(s): D6W3Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: September 7, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.