Reviewed, UniProtKB/Swiss-Prot P06776 (PDE2_YEAST)

Names and origin

Protein names

Recommended name:
    3',5'-cyclic-nucleotide phosphodiesterase 2
      Short name=PDEase 2
    EC=3.1.4.17
Alternative name(s):
    High-affinity cAMP phosphodiesterase

Gene names

Name:	PDE2
Synonyms:	SRA5
Ordered Locus Names:	YOR360C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Controls the level of cAMP in yeast cells, together with the low-affinity cAMP phosphodiesterase (PDE1).
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Subunit structure	Monomer.
Miscellaneous	Present with 6510 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords
Ligand	cAMP
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cAMP-mediated signaling Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	3',5'-cyclic-AMP phosphodiesterase activity Ref.1 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MET31	Q03081	1	EBI-4780,EBI-11511
NOC2	P39744	1	EBI-4780,EBI-29259

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	3',5'-cyclic-nucleotide phosphodiesterase 2		PRO_0000198851
Experimental info
	Sequence conflict	82	1	R → G in AAA34846. Ref.1
	Sequence conflict	349 – 350	2	QL → LK in AAA34846. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P06776-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 2058659DA87F286B	FASTA	526	61,000
	10 20 30 40 50 60 MSTLFLIGIH EIEKSQTIVQ NEHYFDRVIE LQDLDSLMVA LYKDRVSPFP NVHNFETGVS 70 80 90 100 110 120 IVLYDPSKFQ LSVRQLDVLF KRFFPSFNIS AIDHTREENL QRLECVEREN SICRNRITRI 130 140 150 160 170 180 NHWMYHHHDD TPDGINKNSY GTVNGNSVPT QACEANIYTL LLHLNDSKAQ HLRKASVPRL 190 200 210 220 230 240 IRNIEFMSFL SDPIEKISQE GSHYWNILST WDFCALSLST QELIWCGFTL IKKLSKDAKV 250 260 270 280 290 300 LIADNKLLLL LFTLESSYHQ VNKFHNFRHA IDVMQATWRL CTYLLKDNPV QTLLLCMAAI 310 320 330 340 350 360 GHDVGHPGTN NQLLCNCESE VAQNFKNVSI LENFHRELFQ QLLSEHWPQL LSISKKKFDF 370 380 390 400 410 420 ISEAILATDM ALHSQYEDRL MHENPMKQIT LISLIIKAAD ISNVTRTLSI SARWAYLITL 430 440 450 460 470 480 EFNDCALLET FHKAHRPEQD CFGDSYKNVD SPKEDLESIQ NILVNVTDPD DIIKDHPHIP 490 500 510 520 NGQIFFINTF AEVFFNALSQ KFSGLKFLSD NVKINKEYWM KHKKPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae." Sass P., Field J., Nikawa J., Toda T., Wigler M. Proc. Natl. Acad. Sci. U.S.A. 83:9303-9307(1986) [PubMed: 3025832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M14563 Genomic DNA. Translation: AAA34846.1. Z75268 Genomic DNA. Translation: CAA99689.1.
PIR	S67272.
RefSeq	NP_015005.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4055N.
IntAct	P06776. 21 interactions.
Proteomic databases
PeptideAtlas	P06776.
Genome annotation databases
Ensembl	YOR360C. Saccharomyces cerevisiae. [Contig view]
GeneID	854542.
GenomeReviews	Gene locus YOR360C in contig Y13140_GR.
KEGG	sce:YOR360C.
NMPDR	fig|4932.3.peg.6124.
Organism-specific databases
CYGD	YOR360c.
SGD	S000005887. PDE2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P06776.
OMA	P06776. ATDMALH.
Enzyme and pathway databases
BRENDA	3.1.4.17. 250.
Gene expression databases
GermOnline	YOR360C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976949.

Entry information

Entry name

PDE2_YEAST

Accession

Primary (citable) accession number: P06776
Secondary accession number(s): Q08836

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)