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P06776

- PDE2_YEAST

UniProt

P06776 - PDE2_YEAST

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Protein

3',5'-cyclic-nucleotide phosphodiesterase 2

Gene

PDE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Controls the level of cAMP in yeast cells, together with the low-affinity cAMP phosphodiesterase (PDE1).

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei265 – 2651Proton donorBy similarity
Metal bindingi269 – 2691Divalent metal cation 1By similarity
Metal bindingi302 – 3021Divalent metal cation 1By similarity
Metal bindingi303 – 3031Divalent metal cation 1By similarity
Metal bindingi303 – 3031Divalent metal cation 2By similarity
Metal bindingi400 – 4001Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cAMP-mediated signaling Source: SGD
  2. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YOR360C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
3',5'-cyclic-nucleotide phosphodiesterase 2 (EC:3.1.4.17)
Short name:
PDEase 2
Alternative name(s):
High-affinity cAMP phosphodiesterase
Gene namesi
Name:PDE2
Synonyms:SRA5
Ordered Locus Names:YOR360C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR360c.
SGDiS000005887. PDE2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5265263',5'-cyclic-nucleotide phosphodiesterase 2PRO_0000198851Add
BLAST

Proteomic databases

MaxQBiP06776.
PaxDbiP06776.
PeptideAtlasiP06776.

Expressioni

Gene expression databases

GenevestigatoriP06776.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi34745. 237 interactions.
DIPiDIP-4055N.
IntActiP06776. 3 interactions.
MINTiMINT-498200.
STRINGi4932.YOR360C.

Structurei

3D structure databases

ProteinModelPortaliP06776.
SMRiP06776. Positions 176-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG282089.
HOGENOMiHOG000066009.
KOiK01120.
OMAiKFHNFRH.
OrthoDBiEOG71RXW4.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06776-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTLFLIGIH EIEKSQTIVQ NEHYFDRVIE LQDLDSLMVA LYKDRVSPFP
60 70 80 90 100
NVHNFETGVS IVLYDPSKFQ LSVRQLDVLF KRFFPSFNIS AIDHTREENL
110 120 130 140 150
QRLECVEREN SICRNRITRI NHWMYHHHDD TPDGINKNSY GTVNGNSVPT
160 170 180 190 200
QACEANIYTL LLHLNDSKAQ HLRKASVPRL IRNIEFMSFL SDPIEKISQE
210 220 230 240 250
GSHYWNILST WDFCALSLST QELIWCGFTL IKKLSKDAKV LIADNKLLLL
260 270 280 290 300
LFTLESSYHQ VNKFHNFRHA IDVMQATWRL CTYLLKDNPV QTLLLCMAAI
310 320 330 340 350
GHDVGHPGTN NQLLCNCESE VAQNFKNVSI LENFHRELFQ QLLSEHWPQL
360 370 380 390 400
LSISKKKFDF ISEAILATDM ALHSQYEDRL MHENPMKQIT LISLIIKAAD
410 420 430 440 450
ISNVTRTLSI SARWAYLITL EFNDCALLET FHKAHRPEQD CFGDSYKNVD
460 470 480 490 500
SPKEDLESIQ NILVNVTDPD DIIKDHPHIP NGQIFFINTF AEVFFNALSQ
510 520
KFSGLKFLSD NVKINKEYWM KHKKPQ
Length:526
Mass (Da):61,000
Last modified:November 1, 1997 - v2
Checksum:i2058659DA87F286B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821R → G in AAA34846. (PubMed:3025832)Curated
Sequence conflicti349 – 3502QL → LK in AAA34846. (PubMed:3025832)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14563 Genomic DNA. Translation: AAA34846.1.
Z75268 Genomic DNA. Translation: CAA99689.1.
BK006948 Genomic DNA. Translation: DAA11121.1.
PIRiS67272.
RefSeqiNP_015005.1. NM_001183780.1.

Genome annotation databases

EnsemblFungiiYOR360C; YOR360C; YOR360C.
GeneIDi854542.
KEGGisce:YOR360C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14563 Genomic DNA. Translation: AAA34846.1 .
Z75268 Genomic DNA. Translation: CAA99689.1 .
BK006948 Genomic DNA. Translation: DAA11121.1 .
PIRi S67272.
RefSeqi NP_015005.1. NM_001183780.1.

3D structure databases

ProteinModelPortali P06776.
SMRi P06776. Positions 176-521.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34745. 237 interactions.
DIPi DIP-4055N.
IntActi P06776. 3 interactions.
MINTi MINT-498200.
STRINGi 4932.YOR360C.

Proteomic databases

MaxQBi P06776.
PaxDbi P06776.
PeptideAtlasi P06776.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR360C ; YOR360C ; YOR360C .
GeneIDi 854542.
KEGGi sce:YOR360C.

Organism-specific databases

CYGDi YOR360c.
SGDi S000005887. PDE2.

Phylogenomic databases

eggNOGi NOG282089.
HOGENOMi HOG000066009.
KOi K01120.
OMAi KFHNFRH.
OrthoDBi EOG71RXW4.

Enzyme and pathway databases

BioCyci YEAST:YOR360C-MONOMER.

Miscellaneous databases

NextBioi 976949.

Gene expression databases

Genevestigatori P06776.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae."
    Sass P., Field J., Nikawa J., Toda T., Wigler M.
    Proc. Natl. Acad. Sci. U.S.A. 83:9303-9307(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPDE2_YEAST
AccessioniPrimary (citable) accession number: P06776
Secondary accession number(s): D6W355, Q08836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6510 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3