P06773 (DCTD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxycytidylate deaminase
EC=3.5.4.12

Alternative name(s):
dCMP deaminase

Gene names

Name:	DCD1
Ordered Locus Names:	YHR144C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Supplies the nucleotide substrate for thymidylate synthetase.
Catalytic activity	dCMP + H2O = dUMP + NH3.
Cofactor	Zinc By similarity.
Enzyme regulation	Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.
Miscellaneous	Present with 1510 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	dCMP deaminase activity Inferred from genetic interaction. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Deoxycytidylate deaminase		PRO_0000171697
Sites
	Active site	235	1	Proton donor By similarity
	Metal binding	233	1	Zinc; catalytic By similarity
	Metal binding	260	1	Zinc; catalytic By similarity
	Metal binding	263	1	Zinc; catalytic By similarity
Experimental info
	Sequence conflict	78	1	V → A in AAA34561. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P06773 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 780B47CDA561D24B	FASTA	312	35,646
	10 20 30 40 50 60 MLIGVSGTKF CGCEDVINML VDHFHFELLN HLDNPEEILD YATKNYTKNS VIFLEKLSLL 70 80 90 100 110 120 EKLEKRPFFV HLSIDAPVTT RVALYRKTTQ AESLSLEQII QAIDQHDFQP EGIKLREKSH 130 140 150 160 170 180 LRFKIVNEDR RGRRQSLINN ITTQLKILDD KEKQMAPLMR PSWDSYFMKL ATLAASRSNC 190 200 210 220 230 240 MKRRVGCVIV RECRVIATGY NGTPRHLTNC FNGGCPRCND GDSRNLHTCL CLHAEENALL 250 260 270 280 290 300 EAGRDRVGQN ATLYCDTCPC LTCSVKIVQT GISEVVYSQS YRMDEESFKV LKNAGITVRQ 310 FSFTEEPRIV MI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression of the dCMP deaminase gene (DCD1) of Saccharomyces cerevisiae." McIntosh E.M., Haynes R.H. Mol. Cell. Biol. 6:1711-1721(1986) [PubMed: 3023902] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M13010 Genomic DNA. Translation: AAA34561.1. U10397 Genomic DNA. Translation: AAB68985.1. BK006934 Genomic DNA. Translation: DAA06838.1.
PIR	S46762.
RefSeq	NP_012014.1. NM_001179275.1.
3D structure databases
ProteinModelPortal	P06773.
SMR	P06773. Positions 2-44, 159-306.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4028N.
MINT	MINT-498279.
STRING	P06773.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR144C; YHR144C; YHR144C.
GeneID	856548.
KEGG	sce:YHR144C.
NMPDR	fig|4932.3.peg.3178.
Organism-specific databases
CYGD	YHR144c.
SGD	S000001187. DCD1.
Phylogenomic databases
eggNOG	fuNOG07530.
GeneTree	EFGT00050000006350.
HOGENOM	HBG330619.
OMA	ERTTIHI.
OrthoDB	EOG45F0ZM.
Gene expression databases
ArrayExpress	P06773.
Genevestigator	P06773.
GermOnline	YHR144C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016192. APOBEC/CMP_deaminase_Zn-bd. IPR002125. CMP_dCMP_Zn-bd. IPR015517. Cyt_deaminase. IPR016193. Cytidine_deaminase-like. [Graphical view]
KO	K01493.
PANTHER	PTHR11086. Cyt_deaminase. 1 hit.
Pfam	PF00383. dCMP_cyt_deam_1. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit.
PROSITE	PS00903. CYT_DCMP_DEAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982352.

Entry information

Entry name

DCTD_YEAST

Accession

Primary (citable) accession number: P06773
Secondary accession number(s): D3DL94

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program