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Protein

Deoxycytidylate deaminase

Gene

DCD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Supplies the nucleotide substrate for thymidylate synthetase.

Catalytic activityi

dCMP + H2O = dUMP + NH3.

Cofactori

Zn2+By similarity

Enzyme regulationi

Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi233 – 2331Zinc; catalyticBy similarity
Active sitei235 – 2351Proton donorBy similarity
Metal bindingi260 – 2601Zinc; catalyticBy similarity
Metal bindingi263 – 2631Zinc; catalyticBy similarity

GO - Molecular functioni

  1. dCMP deaminase activity Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. dTMP biosynthetic process Source: SGD
  2. dUMP biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YHR144C-MONOMER.
YEAST:YHR144C-MONOMER.
ReactomeiREACT_259872. Pyrimidine biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxycytidylate deaminase (EC:3.5.4.12)
Alternative name(s):
dCMP deaminase
Gene namesi
Name:DCD1
Ordered Locus Names:YHR144C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR144c.
SGDiS000001187. DCD1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Deoxycytidylate deaminasePRO_0000171697Add
BLAST

Proteomic databases

MaxQBiP06773.
PaxDbiP06773.
PRIDEiP06773.

Expressioni

Gene expression databases

GenevestigatoriP06773.

Interactioni

Protein-protein interaction databases

BioGridi36578. 19 interactions.
DIPiDIP-4028N.
IntActiP06773. 1 interaction.
MINTiMINT-498279.
STRINGi4932.YHR144C.

Structurei

3D structure databases

ProteinModelPortaliP06773.
SMRiP06773. Positions 160-283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2131.
GeneTreeiENSGT00390000000501.
HOGENOMiHOG000217578.
InParanoidiP06773.
KOiK01493.
OMAiERTTIHI.
OrthoDBiEOG7BCNPR.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR015517. dCMP_deaminase-rel.
[Graphical view]
PANTHERiPTHR11086. PTHR11086. 1 hit.
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P06773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIGVSGTKF CGCEDVINML VDHFHFELLN HLDNPEEILD YATKNYTKNS
60 70 80 90 100
VIFLEKLSLL EKLEKRPFFV HLSIDAPVTT RVALYRKTTQ AESLSLEQII
110 120 130 140 150
QAIDQHDFQP EGIKLREKSH LRFKIVNEDR RGRRQSLINN ITTQLKILDD
160 170 180 190 200
KEKQMAPLMR PSWDSYFMKL ATLAASRSNC MKRRVGCVIV RECRVIATGY
210 220 230 240 250
NGTPRHLTNC FNGGCPRCND GDSRNLHTCL CLHAEENALL EAGRDRVGQN
260 270 280 290 300
ATLYCDTCPC LTCSVKIVQT GISEVVYSQS YRMDEESFKV LKNAGITVRQ
310
FSFTEEPRIV MI
Length:312
Mass (Da):35,646
Last modified:February 1, 1995 - v2
Checksum:i780B47CDA561D24B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781V → A in AAA34561 (PubMed:3023902).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13010 Genomic DNA. Translation: AAA34561.1.
U10397 Genomic DNA. Translation: AAB68985.1.
BK006934 Genomic DNA. Translation: DAA06838.1.
PIRiS46762.
RefSeqiNP_012014.1. NM_001179275.1.

Genome annotation databases

EnsemblFungiiYHR144C; YHR144C; YHR144C.
GeneIDi856548.
KEGGisce:YHR144C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13010 Genomic DNA. Translation: AAA34561.1.
U10397 Genomic DNA. Translation: AAB68985.1.
BK006934 Genomic DNA. Translation: DAA06838.1.
PIRiS46762.
RefSeqiNP_012014.1. NM_001179275.1.

3D structure databases

ProteinModelPortaliP06773.
SMRiP06773. Positions 160-283.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36578. 19 interactions.
DIPiDIP-4028N.
IntActiP06773. 1 interaction.
MINTiMINT-498279.
STRINGi4932.YHR144C.

Proteomic databases

MaxQBiP06773.
PaxDbiP06773.
PRIDEiP06773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR144C; YHR144C; YHR144C.
GeneIDi856548.
KEGGisce:YHR144C.

Organism-specific databases

CYGDiYHR144c.
SGDiS000001187. DCD1.

Phylogenomic databases

eggNOGiCOG2131.
GeneTreeiENSGT00390000000501.
HOGENOMiHOG000217578.
InParanoidiP06773.
KOiK01493.
OMAiERTTIHI.
OrthoDBiEOG7BCNPR.

Enzyme and pathway databases

BioCyciMetaCyc:YHR144C-MONOMER.
YEAST:YHR144C-MONOMER.
ReactomeiREACT_259872. Pyrimidine biosynthesis.

Miscellaneous databases

NextBioi982352.

Gene expression databases

GenevestigatoriP06773.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR015517. dCMP_deaminase-rel.
[Graphical view]
PANTHERiPTHR11086. PTHR11086. 1 hit.
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and expression of the dCMP deaminase gene (DCD1) of Saccharomyces cerevisiae."
    McIntosh E.M., Haynes R.H.
    Mol. Cell. Biol. 6:1711-1721(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDCTD_YEAST
AccessioniPrimary (citable) accession number: P06773
Secondary accession number(s): D3DL94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1510 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.