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Protein

Deoxycytidylate deaminase

Gene

DCD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Supplies the nucleotide substrate for thymidylate synthetase.

Miscellaneous

Present with 1510 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

dCMP + H2O = dUMP + NH3.

Cofactori

Zn2+By similarity

Enzyme regulationi

Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi233Zinc; catalyticBy similarity1
Active sitei235Proton donorBy similarity1
Metal bindingi260Zinc; catalyticBy similarity1
Metal bindingi263Zinc; catalyticBy similarity1

GO - Molecular functioni

  • dCMP deaminase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • dTMP biosynthetic process Source: SGD
  • dUMP biosynthetic process Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processNucleotide biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YHR144C-MONOMER.
YEAST:YHR144C-MONOMER.
ReactomeiR-SCE-499943. Interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxycytidylate deaminase (EC:3.5.4.12)
Alternative name(s):
dCMP deaminase
Gene namesi
Name:DCD1
Ordered Locus Names:YHR144C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR144C.
SGDiS000001187. DCD1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001716971 – 312Deoxycytidylate deaminaseAdd BLAST312

Proteomic databases

MaxQBiP06773.
PRIDEiP06773.

Interactioni

Protein-protein interaction databases

BioGridi36578. 125 interactors.
DIPiDIP-4028N.
IntActiP06773. 1 interactor.
MINTiMINT-498279.
STRINGi4932.YHR144C.

Structurei

3D structure databases

ProteinModelPortaliP06773.
SMRiP06773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 291CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST130

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000000501.
HOGENOMiHOG000217578.
InParanoidiP06773.
KOiK01493.
OMAiRCNDGDS.
OrthoDBiEOG092C46M0.

Family and domain databases

CDDicd01286. deoxycytidylate_deaminase. 1 hit.
InterProiView protein in InterPro
IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_dom.
IPR016193. Cytidine_deaminase-like.
IPR015517. dCMP_deaminase-rel.
IPR035105. Deoxycytidylate_deaminase_dom.
PANTHERiPTHR11086. PTHR11086. 1 hit.
PfamiView protein in Pfam
PF00383. dCMP_cyt_deam_1. 1 hit.
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiView protein in PROSITE
PS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P06773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIGVSGTKF CGCEDVINML VDHFHFELLN HLDNPEEILD YATKNYTKNS
60 70 80 90 100
VIFLEKLSLL EKLEKRPFFV HLSIDAPVTT RVALYRKTTQ AESLSLEQII
110 120 130 140 150
QAIDQHDFQP EGIKLREKSH LRFKIVNEDR RGRRQSLINN ITTQLKILDD
160 170 180 190 200
KEKQMAPLMR PSWDSYFMKL ATLAASRSNC MKRRVGCVIV RECRVIATGY
210 220 230 240 250
NGTPRHLTNC FNGGCPRCND GDSRNLHTCL CLHAEENALL EAGRDRVGQN
260 270 280 290 300
ATLYCDTCPC LTCSVKIVQT GISEVVYSQS YRMDEESFKV LKNAGITVRQ
310
FSFTEEPRIV MI
Length:312
Mass (Da):35,646
Last modified:February 1, 1995 - v2
Checksum:i780B47CDA561D24B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78V → A in AAA34561 (PubMed:3023902).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13010 Genomic DNA. Translation: AAA34561.1.
U10397 Genomic DNA. Translation: AAB68985.1.
BK006934 Genomic DNA. Translation: DAA06838.1.
PIRiS46762.
RefSeqiNP_012014.1. NM_001179275.1.

Genome annotation databases

EnsemblFungiiYHR144C; YHR144C; YHR144C.
GeneIDi856548.
KEGGisce:YHR144C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDCTD_YEAST
AccessioniPrimary (citable) accession number: P06773
Secondary accession number(s): D3DL94
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: February 1, 1995
Last modified: July 5, 2017
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names