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P06769 (NIFD_RHICP) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrogenase molybdenum-iron protein alpha chain

EC=1.18.6.1
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene names
Name:nifD
OrganismRhizobium sp. cowpea (strain IRc78)
Taxonomic identifier400 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activity

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactor

Binds 1 8Fe-7S cluster per heterodimer By similarity.

Binds 1 7Fe-Mo-9S-C-homocitryl cluster per subunit By similarity.

Subunit structure

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Sequence similarities

Belongs to the NifD/NifK/NifE/NifN family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Nitrogenase molybdenum-iron protein alpha chain
PRO_0000153078

Sites

Metal binding671Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding931Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding1591Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding2831Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl) By similarity
Metal binding4511Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogen By similarity

Secondary structure

..................................................................................... 500
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P06769 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 5F7FA8DDE2174CDF

FASTA50056,136
        10         20         30         40         50         60 
MSLASTQSIA EIRARNKELI QEVLKVYPEK TAKRRAKHLN VHQAGKSDCG VKSNIKSIPG 

        70         80         90        100        110        120 
VMTIRGCAYA GSKGVVWGPI KDMVHISHGP VGCGQYSWGS RRNYYVGTTG IDSFVTLQFT 

       130        140        150        160        170        180 
FDFREKDIVF GGDKKLVKIL DEIQELFPLN NGITIQSECP IGLIGDDIEA VSRAKSKEYG 

       190        200        210        220        230        240 
GKTIVPVRCE GFRGVSQSLG HHIANDAVRD WIFDQVEADG KPKVEPTPYD VAIIGDYNIG 

       250        260        270        280        290        300 
GDAWSSRILL EEMGLRVIAQ WSGDGSLAEL EANVEGKLNI LHCYRSMNYI SRHMEEKFGI 

       310        320        330        340        350        360 
PWCEYNFFGP SKIAESLRRI AGYFDDKIKE GAERVIEKYQ PLVNAVIAKY RPRLEGKTVM 

       370        380        390        400        410        420 
LYVGGLRSRH VIGAYEDLGM EVIGTGYEFG HNDDYQRTAQ HYVKDGTLIH DDVNGYEFER 

       430        440        450        460        470        480 
FVEKLQPDLV GSGIKEKYVF QKMGGPFRQM HSWDYSGPYH GYDGFAIFAR DMDMAINSPV 

       490        500 
WKKTKAPWKE ASRAKLLAAE 

« Hide

References

[1]"Structural genes of dinitrogenase and dinitrogenase reductase are transcribed from two separate promoters in the broad host range cowpea Rhizobium strain IRc78."
Yun A.C., Szalay A.A.
Proc. Natl. Acad. Sci. U.S.A. 81:7358-7362(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M10203 Genomic DNA. Translation: AAA26308.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFXmodel-A1-500[»]
ProteinModelPortalP06769.
SMRP06769. Positions 13-489.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000510. Nase/OxRdtase_comp1.
IPR010143. Nase_comp1_asu.
IPR000318. Nase_comp1_CS.
IPR005972. Nase_Mo-Fe_asu.
[Graphical view]
PfamPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsTIGR01862. N2-ase-Ialpha. 1 hit.
TIGR01282. nifD. 1 hit.
PROSITEPS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIFD_RHICP
AccessionPrimary (citable) accession number: P06769
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: January 22, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references