Protachykinin-1 precursor - Rattus norvegicus (Rat)

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P06767 (TKN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protachykinin-1

Alternative name(s):
PPT

Cleaved into the following 5 chains:

Substance P
Neurokinin A
Short name=NKA
Alternative name(s):
Neuromedin L
Substance K
Neuropeptide K
Short name=NPK
Neuropeptide gamma
C-terminal-flanking peptide

Gene names

Name:	Tac1
Synonyms:	Nka, Nkna, Tac2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	130 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Subcellular location	Secreted.
Sequence similarities	Belongs to the tachykinin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Neuropeptide Neurotransmitter
PTM	Amidation Cleavage on pair of basic residues
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	associative learning Inferred from direct assay. Source: RGD elevation of cytosolic calcium ion concentration Inferred from direct assay. Source: RGD long-term memory Inferred from direct assay. Source: RGD negative regulation of heart rate Inferred from direct assay. Source: RGD neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of action potential Inferred from direct assay. Source: RGD positive regulation of acute inflammatory response Inferred from direct assay. Source: RGD positive regulation of corticosterone secretion Inferred from direct assay. Source: RGD positive regulation of epithelial cell migration Inferred from direct assay. Source: RGD positive regulation of lymphocyte proliferation Inferred from mutant phenotype. Source: RGD positive regulation of renal sodium excretion Inferred from direct assay. Source: RGD positive regulation of saliva secretion Inferred from direct assay. Source: RGD positive regulation of stress fiber assembly Inferred from direct assay. Source: RGD positive regulation of synaptic transmission, GABAergic Inferred from direct assay. Source: RGD positive regulation of synaptic transmission, cholinergic Inferred from direct assay. Source: RGD regulation of blood pressure Inferred from direct assay. Source: RGD response to hormone stimulus Inferred from mutant phenotype. Source: RGD response to lipopolysaccharide Inferred from expression pattern. Source: RGD response to morphine Inferred from expression pattern. Source: RGD synaptic transmission Inferred from electronic annotation. Source: UniProtKB-KW tachykinin receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from direct assay. Source: RGD plasma membrane Inferred from direct assay. Source: RGD
Molecular function	substance P receptor binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform Beta (identifier: P06767-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Alpha (identifier: P06767-2) The sequence of this isoform differs from the canonical sequence as follows: 97-114: Missing. 115-115: V → M

Isoform Gamma (identifier: P06767-3) The sequence of this isoform differs from the canonical sequence as follows: 74-88: Missing.

Isoform Delta (identifier: P06767-4) The sequence of this isoform differs from the canonical sequence as follows: 74-88: Missing. 97-114: Missing. 115-115: V → M

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

Potential

Propeptide

20 – 56

Potential

PRO_0000033550

Peptide

58 – 68

Substance P

PRO_0000033551

Peptide

72 – 107

Neuropeptide K

PRO_0000033552

Peptide

72 – 73

Neuropeptide gamma, 1st part

PRO_0000033553

Peptide

89 – 107

Neuropeptide gamma, 2nd part

PRO_0000033554

Peptide

98 – 107

Neurokinin A

PRO_0000033555

Peptide

111 – 126

C-terminal-flanking peptide

PRO_0000033556

Amino acid modifications

Modified residue

Methionine amide By similarity

Modified residue

107

Methionine amide By similarity

Natural variations

Alternative sequence

74 – 88

Missing in isoform Gamma and isoform Delta.

VSP_006380

Alternative sequence

97 – 114

Missing in isoform Alpha and isoform Delta.

VSP_006381

Alternative sequence

115

V → M in isoform Alpha and isoform Delta.

VSP_006382

Sequences

Sequence		Length	Mass (Da)
Isoform Beta [UniParc]. Last modified May 30, 2000. Version 2. Checksum: B22EFE860DCCD75A Show »	FASTA	130	15,001
10 20 30 40 50 60 MKILVAVAVF FLVSTQLFAE EIGANDDLNY WSDWSDSDQI KEAMPEPFEH LLQRIARRPK 70 80 90 100 110 120 PQQFFGLMGK RDADSSIEKQ VALLKALYGH GQISHKRHKT DSFVGLMGKR ALNSVAYERS 130 AMQNYERRRK « Hide
Isoform Alpha [UniParc]. Checksum: 5B21D8FED8E0D76E Show »	FASTA	112	13,034
10 20 30 40 50 60 MKILVAVAVF FLVSTQLFAE EIGANDDLNY WSDWSDSDQI KEAMPEPFEH LLQRIARRPK 70 80 90 100 110 PQQFFGLMGK RDADSSIEKQ VALLKALYGH GQISHKMAYE RSAMQNYERR RK « Hide
Isoform Gamma [UniParc]. Checksum: 83E021DCFF1F01F0 Show »	FASTA	115	13,341
10 20 30 40 50 60 MKILVAVAVF FLVSTQLFAE EIGANDDLNY WSDWSDSDQI KEAMPEPFEH LLQRIARRPK 70 80 90 100 110 PQQFFGLMGK RDAGHGQISH KRHKTDSFVG LMGKRALNSV AYERSAMQNY ERRRK « Hide
Isoform Delta [UniParc]. Checksum: C2A5A1F3565232A2 Show »	FASTA	97	11,374
10 20 30 40 50 60 MKILVAVAVF FLVSTQLFAE EIGANDDLNY WSDWSDSDQI KEAMPEPFEH LLQRIARRPK 70 80 90 PQQFFGLMGK RDAGHGQISH KMAYERSAMQ NYERRRK « Hide

References

[1]	"Structure, expression, and some regulatory mechanisms of the rat preprotachykinin gene encoding substance P, neurokinin A, neuropeptide K, and neuropeptide gamma." Carter M.S., Krause J.E. J. Neurosci. 10:2203-2214(1990) [PubMed: 1695945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS ALPHA; BETA AND GAMMA).
[2]	"Three rat preprotachykinin mRNAs encode the neuropeptides substance P and neurokinin A." Krause J.E., Chirgwin J.M., Carter M.S., Xu Z.S., Hershey A.D. Proc. Natl. Acad. Sci. U.S.A. 84:881-885(1987) [PubMed: 2433692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA AND GAMMA).
[3]	"Sequence analysis of cloned cDNA for rat substance P precursor: existence of a third substance P precursor." Kawaguchi Y., Hoshimaru M., Nawa H., Nakanishi S. Biochem. Biophys. Res. Commun. 139:1040-1046(1986) [PubMed: 2429656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA).
[4]	"Identification and cDNA sequence of delta-preprotachykinin, a fourth splicing variant of the rat substance P precursor." Harmar A.J., Hyde V., Chapman K.E. FEBS Lett. 275:22-24(1990) [PubMed: 1702066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM DELTA). Tissue: Spinal ganglion.
[5]	"The sequence of 5' flanking DNA from the rat preprotachykinin gene; analysis of putative transcription factor binding sites." Chapman K.E., Lyons V., Harmar A.J. Biochim. Biophys. Acta 1172:361-363(1993) [PubMed: 8448217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-41.
[6]	"Fourth isoform of preprotachykinin messenger RNA encoding for substance P in the rat intestine." Khan I., Collins S.M. Biochem. Biophys. Res. Commun. 202:796-802(1994) [PubMed: 7519424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-125 (ISOFORM DELTA).
[7]	"Quantitation and characterization of peptides from the C-terminal flanking region of rat and bovine preprotachykinins." McGregor G.P., Kage R., Thim L., Conlon J.M. J. Neurochem. 53:1871-1877(1989) [PubMed: 2809597] [Abstract] Cited for: IDENTIFICATION OF C-TERMINAL-FLANKING PEPTIDE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34162 , M34159, M34160, M34161 Genomic DNA. Translation: AAA41926.1. M34184 mRNA. Translation: AAA41925.1. M34183 mRNA. Translation: AAA41929.1. M15191 mRNA. Translation: AAA41928.1. M14312 mRNA. Translation: AAA41927.1. L07328 Genomic DNA. Translation: AAA41924.1. X56306 mRNA. Translation: CAA39752.1. S72369 mRNA. Translation: AAB31499.1.
IPI	IPI00231091. IPI00232007. IPI00232008. IPI00232009.
PIR	SPRTB. A37163. SPRTA. B26590. S12958.
RefSeq	NP_001118240.1. NM_001124768.1. NP_001118241.1. NM_001124769.1. NP_001118242.1. NM_001124770.1. NP_036798.1. NM_012666.2.
UniGene	Rn.1920.
3D structure databases
ProteinModelPortal	P06767.
SMR	P06767. Positions 72-107.
ModBase	Search...
Protein-protein interaction databases
STRING	P06767.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000009888; ENSRNOP00000009888; ENSRNOG00000007374. ENSRNOT00000034719; ENSRNOP00000033246; ENSRNOG00000007374. ENSRNOT00000052333; ENSRNOP00000048764; ENSRNOG00000007374.
GeneID	24806.
KEGG	rno:24806.
UCSC	NM_012666. rat.
Organism-specific databases
CTD	6863.
RGD	3807. Tac1.
Phylogenomic databases
eggNOG	roNOG17222.
GeneTree	ENSGT00390000002457.
HOVERGEN	HBG000127.
InParanoid	P06767.
OMA	EMGANDD.
OrthoDB	EOG444KMW.
PhylomeDB	P06767.
Gene expression databases
ArrayExpress	P06767.
Genevestigator	P06767.
GermOnline	ENSRNOG00000007374. Rattus norvegicus.
Family and domain databases
InterPro	IPR008216. Protachykinin. IPR013055. Tachy_Neuro_lke_CS. IPR008215. Tachykinin. [Graphical view]
KO	K05239.
PANTHER	PTHR11250. Protachykinin. 1 hit.
PRINTS	PR01829. PROTACHYKNIN.
SMART	SM00203. TK. 2 hits. [Graphical view]
PROSITE	PS00267. TACHYKININ. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	604480.
PMAP-CutDB	P06767.

Entry information

Entry name

TKN1_RAT

Accession

Primary (citable) accession number: P06767
Secondary accession number(s): P08856

Q6LD93

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 5 chains:

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents