P06761 (GRP78_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
78 kDa glucose-regulated protein
Short name=GRP-78

Alternative name(s):
Heat shock 70 kDa protein 5
Immunoglobulin heavy chain-binding protein
Short name=BiP
Steroidogenesis-activator polypeptide

Gene names

Name:	Hspa5
Synonyms:	Grp78

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	654 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Subunit structure	May form a complex with ERLEC1, OS9, SEL1L and SYVN1 By similarity. Interacts with DNAJC1 (via J domain). Interacts with TRIM21 By similarity. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A. Interacts with MX1 By similarity. Ref.7
Subcellular location	Endoplasmic reticulum lumen. Melanosome By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ER overload response Inferred from electronic annotation. Source: Compara activation of signaling protein activity involved in unfolded protein response Inferred from electronic annotation. Source: Compara cellular response to glucose starvation Inferred from electronic annotation. Source: Compara cerebellar Purkinje cell layer development Inferred from electronic annotation. Source: Compara cerebellum structural organization Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of protein ubiquitination Inferred from electronic annotation. Source: Compara proteolysis involved in cellular protein catabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara endoplasmic reticulum Inferred from direct assay PubMed 19474315. Source: MGI endoplasmic reticulum chaperone complex Inferred from electronic annotation. Source: Compara endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum-Golgi intermediate compartment Inferred from electronic annotation. Source: Compara integral to endoplasmic reticulum membrane Inferred from electronic annotation. Source: Compara melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell midbody Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara signalosome Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type endopeptidase inhibitor activity involved in apoptotic process Inferred from electronic annotation. Source: Compara ribosome binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Sigmar1	Q9R0C9	3	EBI-916036,EBI-1557826
Tg	P06882	6	EBI-916036,EBI-1549657

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Chain	19 – 654	636	78 kDa glucose-regulated protein		PRO_0000013569
Regions
	Motif	651 – 654	4	Prevents secretion from ER
Amino acid modifications
	Modified residue	166	1	Phosphothreonine By similarity
	Modified residue	518	1	Phosphothreonine By similarity
	Modified residue	571	1	Phosphoserine By similarity
	Modified residue	649	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	29	1	T → M in AAA41277. Ref.3
	Sequence conflict	649	1	S → D AA sequence Ref.5
	Sequence conflict	651	1	K → KK AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P06761 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 9D686C6484150108	FASTA	654	72,347
	10 20 30 40 50 60 MKFTVVAAAL LLLCAVRAEE EDKKEDVGTV VGIDLGTTYS CVGVFKNGRV EIIANDQGNR 70 80 90 100 110 120 ITPSYVAFTP EGERLIGDAA KNQLTSNPEN TVFDAKRLIG RTWNDPSVQQ DIKFLPFKVV 130 140 150 160 170 180 EKKTKPYIQV DIGGGQTKTF APEEISAMVL TKMKETAEAY LGKKVTHAVV TVPAYFNDAQ 190 200 210 220 230 240 RQATKDAGTI AGLNVMRIIN EPTAAAIAYG LDKREGEKNI LVFDLGGGTF DVSLLTIDNG 250 260 270 280 290 300 VFEVVATNGD THLGGEDFDQ RVMEHFIKLY KKKTGKDVRK DNRAVQKLRR EVEKAKRALS 310 320 330 340 350 360 SQHQARIEIE SFFEGEDFSE TLTRAKFEEL NMDLFRSTMK PVQKVLEDSD LKKSDIDEIV 370 380 390 400 410 420 LVGGSTRIPK IQQLVKEFFN GKEPSRGINP DEAVAYGAAV QAGVLSGDQD TGDLVLLDVC 430 440 450 460 470 480 PLTLGIETVG GVMTKLIPRN TVVPTKKSQI FSTASDNQPT VTIKVYEGER PLTKDNHLLG 490 500 510 520 530 540 TFDLTGIPPA PRGVPQIEVT FEIDVNGILR VTAEDKGTGN KNKITITNDQ NRLTPEEIER 550 560 570 580 590 600 MVNDAEKFAE EDKKLKERID TRNELESYAY SLKNQIGDKE KLGGKLSPED KETMEKAVEE 610 620 630 640 650 KIEWLESHQD ADIEDFKAKK KELEEIVQPI ISKLYGSGGP PPTGEEDTSE KDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein." Munro S., Pelham H.R.B. Cell 46:291-300(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[3]	"Rat gene encoding the 78-kDa glucose-regulated protein GRP78: its regulatory sequences and the effect of protein glycosylation on its expression." Chang S.C., Wooden S.K., Nakaki T., Kim Y.K., Lin A.Y., Kung L., Attenello J.W., Lee A.S. Proc. Natl. Acad. Sci. U.S.A. 84:680-684(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
[4]	Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 50-60; 165-181; 307-324; 475-492 AND 533-540, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
[5]	"Steroidogenesis-activator polypeptide isolated from a rat Leydig cell tumor." Pedersen R.C., Brownie A.C. Science 236:188-190(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 626-654.
[6]	"A subset of chaperones and folding enzymes form multiprotein complexes in endoplasmic reticulum to bind nascent proteins." Meunier L., Usherwood Y.-K., Chung K.T., Hendershot L.M. Mol. Biol. Cell 13:4456-4469(2002) [PubMed] [Europe PMC] [Abstract] Cited for: COMPONENT OF A CHAPERONE COMPLEX.
[7]	"Cloning and characterization of a novel GRP78-binding protein in the rat brain." Oh-hashi K., Naruse Y., Amaya F., Shimosato G., Tanaka M. J. Biol. Chem. 278:10531-10537(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TMEM132A. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14050 mRNA. Translation: AAA40817.1. BC062017 mRNA. Translation: AAH62017.1. M14866 Genomic DNA. Translation: AAA41277.1.
IPI	IPI00206624.
PIR	HHRTGB. A23948.
RefSeq	NP_037215.1. NM_013083.2.
UniGene	Rn.11088.
3D structure databases
ProteinModelPortal	P06761.
SMR	P06761. Positions 28-570.
ModBase	Search...
Protein-protein interaction databases
IntAct	P06761. 6 interactions.
MINT	MINT-4575479.
STRING	10116.ENSRNOP00000025064.
PTM databases
PhosphoSite	P06761.
Proteomic databases
PaxDb	P06761.
PRIDE	P06761.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025067; ENSRNOP00000025064; ENSRNOG00000018294.
GeneID	25617.
KEGG	rno:25617.
UCSC	RGD:2843. rat.
Organism-specific databases
CTD	3309.
RGD	2843. Hspa5.
Phylogenomic databases
eggNOG	COG0443.
GeneTree	ENSGT00550000074467.
HOGENOM	HOG000228135.
HOVERGEN	HBG051845.
InParanoid	P06761.
KO	K09490.
OMA	DKRAVQK.
OrthoDB	EOG444KJV.
Gene expression databases
Genevestigator	P06761.
GermOnline	ENSRNOG00000018294. Rattus norvegicus.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607375.

Entry information

Entry name

GRP78_RAT

Accession

Primary (citable) accession number: P06761

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	April 1, 1988
Last modified:	April 3, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program